FastQCFastQC Report
Fri 27 May 2016
SRR2138988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6751419
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84200.038.1954651
GTATCAA137600.030.801981
TCAACGC158100.026.6415374
ATCAACG158150.026.6331143
CAACGCA160050.026.3443585
AACGCAG165850.025.420996
TATCAAC170800.024.8403972
ACGCAGA191950.022.0329937
CGCAGAG197650.021.3531918
AGAGTAC196550.018.14688310-11
GAGTACT118250.018.12374512-13
GCAGAGT240450.017.479359
CGAGACC62400.017.4219786-87
AGACCGA65750.016.86303388-89
GTACATG171350.016.5156461
TACATGG175200.016.4532322
ACCGAGG67500.016.3440790-91
GAGACCG64950.016.32853188-89
GACCGAG64850.016.2577290-91
CAGAGTA229200.016.25090810-11
Produced by FastQC (version 0.11.5)