##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6751419
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.585576010021004	25.0	18.0	32.0	18.0	33.0
2	26.705704830347518	28.0	18.0	32.0	18.0	33.0
3	28.92037807163205	31.0	27.0	33.0	18.0	33.0
4	29.631369494324083	31.0	29.0	33.0	25.0	33.0
5	31.057807699388825	32.0	32.0	33.0	27.0	33.0
6	36.0498006419095	37.0	36.0	38.0	33.0	38.0
7	36.16822907895362	38.0	37.0	38.0	33.0	38.0
8	36.84419408719856	38.0	38.0	38.0	35.0	38.0
9	37.00193796296749	38.0	38.0	38.0	35.0	38.0
10-11	37.0747724293219	38.0	38.0	38.0	36.0	38.0
12-13	37.12282432774502	38.0	38.0	38.0	36.0	38.0
14-15	37.14364498781663	38.0	38.0	38.0	36.0	38.0
16-17	37.11999136774062	38.0	38.0	38.0	36.0	38.0
18-19	37.15679281644347	38.0	38.0	38.0	36.0	38.0
20-21	37.12366884992379	38.0	38.0	38.0	36.0	38.0
22-23	37.16548009397745	38.0	38.0	38.0	36.0	38.0
24-25	37.17206924445891	38.0	38.0	38.0	36.0	38.0
26-27	37.10430276993951	38.0	38.0	38.0	36.0	38.0
28-29	37.09034508278905	38.0	38.0	38.0	36.0	38.0
30-31	36.95911772959026	38.0	38.0	38.0	36.0	38.0
32-33	37.05709099391821	38.0	38.0	38.0	36.0	38.0
34-35	37.07588689237097	38.0	38.0	38.0	36.0	38.0
36-37	37.002014389057216	38.0	38.0	38.0	36.0	38.0
38-39	37.04090384503904	38.0	38.0	38.0	36.0	38.0
40-41	36.96175439839014	38.0	38.0	38.0	36.0	38.0
42-43	36.944780358524724	38.0	38.0	38.0	36.0	38.0
44-45	36.94602817809406	38.0	38.0	38.0	36.0	38.0
46-47	36.90834700107655	38.0	38.0	38.0	35.5	38.0
48-49	36.87561027035365	38.0	38.0	38.0	35.0	38.0
50-51	36.8929203228364	38.0	38.0	38.0	35.0	38.0
52-53	36.80385358472664	38.0	38.0	38.0	35.0	38.0
54-55	36.787744199591785	38.0	38.0	38.0	35.0	38.0
56-57	36.78912809102155	38.0	38.0	38.0	35.0	38.0
58-59	36.81088939602799	38.0	38.0	38.0	35.0	38.0
60-61	36.8064872682384	38.0	38.0	38.0	35.0	38.0
62-63	36.811061366045735	38.0	38.0	38.0	35.0	38.0
64-65	36.762479240347794	38.0	38.0	38.0	35.0	38.0
66-67	36.76427618104677	38.0	38.0	38.0	35.0	38.0
68-69	36.777766778493394	38.0	38.0	38.0	35.0	38.0
70-71	36.69335986320058	38.0	38.0	38.0	34.5	38.0
72-73	36.64316983329289	38.0	38.0	38.0	34.0	38.0
74-75	36.69442183548361	38.0	38.0	38.0	34.0	38.0
76-77	36.66975993884299	38.0	38.0	38.0	34.0	38.0
78-79	36.686928116733	38.0	38.0	38.0	34.0	38.0
80-81	36.706685452753646	38.0	38.0	38.0	34.0	38.0
82-83	36.57263527618238	38.0	38.0	38.0	34.0	38.0
84-85	36.59168069243226	38.0	38.0	38.0	34.0	38.0
86-87	36.586203605826796	38.0	38.0	38.0	34.0	38.0
88-89	36.532032385115	38.0	38.0	38.0	34.0	38.0
90-91	36.53062131996414	38.0	38.0	38.0	34.0	38.0
92-93	36.57768569980732	38.0	38.0	38.0	34.0	38.0
94-95	36.46812389023718	38.0	38.0	38.0	34.0	38.0
96-97	36.423919958999576	38.0	38.0	38.0	34.0	38.0
98-99	36.49078433450961	38.0	38.0	38.0	34.0	38.0
100	34.884459293040344	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	31.0
19	35.0
20	66.0
21	223.0
22	716.0
23	2139.0
24	4914.0
25	9412.0
26	16978.0
27	26641.0
28	40453.0
29	56391.0
30	77025.0
31	103469.0
32	138234.0
33	191119.0
34	292881.0
35	547559.0
36	1591578.0
37	3651547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.06142367997009	23.790361700258863	14.800755219013958	24.347459400757085
2	13.722626310113473	21.358028586286824	40.15823932716959	24.76110577643011
3	16.279570265154632	26.38940050972988	32.59489597668283	24.73613324843266
4	12.504867495262847	18.91661589956126	39.734935722401474	28.843580882774422
5	13.181796838536105	37.22657286287461	36.11201590019305	13.479614398396233
6	28.600728232094614	39.19066495502649	19.308711250183112	12.899895562695782
7	24.990687142954688	33.196310286770824	24.83691206248642	16.976090507788065
8	23.53454881114622	37.285169236274626	21.938439904263088	17.241842048316062
9	25.03125639217474	16.804719126453268	22.136561217723266	36.02746326364873
10-11	23.45498035301912	27.166429753508115	29.77934712687807	19.599242766594696
12-13	24.257085806702264	24.806866526873833	29.825885491627762	21.110162174796145
14-15	21.699016162380087	26.133513562111904	27.12391276559787	25.04355750991014
16-17	21.323872803628394	29.137185827157225	27.90995492947483	21.628986439739556
18-19	21.3962279633363	28.581213815940025	29.17497047657685	20.84758774414682
20-21	22.24751955579258	27.653495225076856	28.86316562662632	21.235819592504242
22-23	21.998982363183767	27.60999772624088	28.731501636439987	21.659518274135362
24-25	21.847663700230726	27.741805266997755	28.765444169157213	21.64508686361431
26-27	21.767481806251578	27.82221994398094	28.821620522897856	21.58867772686962
28-29	21.676272996100415	27.834978597853784	28.83259942902912	21.65614897701668
30-31	21.963040166373887	27.699452104533435	28.784590400157395	21.552917328935283
32-33	21.915134452037776	27.740501318203798	28.737354906297842	21.607009323460584
34-35	21.886068702942566	27.75221833028865	28.77689954495446	21.584813421814328
36-37	21.90206319518699	27.750276256924305	28.71227319813135	21.63538734975736
38-39	21.75238719050455	27.832676647984876	28.811883668181178	21.6030524933294
40-41	21.883792490633542	27.740231704544012	28.742478513282098	21.633497291540344
42-43	21.84040046026484	27.80268169179218	28.56634950771369	21.79056834022929
44-45	21.815474905159892	27.858655471480535	28.478504803186418	21.84736482017316
46-47	22.012476084405446	27.76831557464916	28.32049972017335	21.898708620772048
48-49	21.915217605814302	27.87740860468395	28.348754143354764	21.85861964614698
50-51	21.827581458443817	27.970803120062065	28.32627665222639	21.87533876926773
52-53	22.017546354281002	27.927974352073782	28.22038259411946	21.834096699525755
54-55	21.985946126298256	27.86487632843484	28.185934381506655	21.963243163760247
56-57	21.98618938513178	27.94562522641668	28.130517499454566	21.937667888996977
58-59	21.844540935637323	27.946322703018346	28.258231703311843	21.950904658032492
60-61	22.117235681700667	27.984845764803744	28.25732382106739	21.64059473242819
62-63	21.90216727211631	28.100410237741873	28.22907889344001	21.768343596701804
64-65	21.899945344138956	28.047768870244294	28.101295742655928	21.950990042960818
66-67	21.86854864379534	28.08530219615163	28.194076310751065	21.852072849301965
68-69	21.835839777224734	28.128855830483	28.099207943033143	21.93609644925912
70-71	21.8977716496078	27.938338216053165	27.97758730423483	22.186302830104207
72-73	21.976499377411717	27.9108665646059	27.979847595937144	22.132786462045235
74-75	21.92564187605016	27.996093288256912	28.060603444052063	22.017661391640864
76-77	22.03458141572406	27.755419521255437	27.9777384399107	22.232260623109802
78-79	21.950782249302573	27.782742696669082	27.8524346126096	22.414040441418745
80-81	22.164415828864833	27.963235894746465	27.792019980669746	22.080328295718953
82-83	22.169819745555465	27.76692976671338	27.89297564091996	22.1702748468112
84-85	22.075598973808436	27.803457762205152	27.850851873668553	22.270091390317855
86-87	21.988470384576456	27.968095056941326	27.892272173612593	22.15116238486963
88-89	22.196935890275174	27.93279821662573	27.812290917327715	22.05797497577138
90-91	22.227931885405372	27.99223201655891	27.79239380514864	21.987442292887074
92-93	22.160660699277287	27.72957791714053	27.8372020520813	22.272559331500887
94-95	22.170022764071096	27.92736562022638	27.88363449867015	22.01897711703237
96-97	22.37004859866221	27.916734653250973	27.980137786348248	21.73307896173857
98-99	23.2010920590284	29.895314834813043	28.287215343970445	18.61637776218811
100	21.964706493556754	30.857556192117592	22.301155634229556	24.876581680096095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	615.0
1	715.5
2	1220.5
3	1779.0
4	2216.0
5	2868.5
6	3406.5
7	3974.0
8	4627.5
9	5182.5
10	5550.5
11	5901.5
12	6766.5
13	8309.5
14	11144.5
15	14587.5
16	16971.0
17	18147.0
18	18293.0
19	17570.5
20	16910.5
21	17205.5
22	19163.5
23	23551.5
24	30712.0
25	40758.0
26	53347.0
27	67906.5
28	84315.0
29	101809.0
30	118343.0
31	135041.5
32	152969.5
33	170548.0
34	185930.0
35	200067.0
36	215635.0
37	229302.5
38	240031.5
39	249742.5
40	258471.0
41	264915.5
42	269077.5
43	273320.0
44	276703.0
45	280634.5
46	286070.0
47	288423.0
48	285724.0
49	275742.0
50	262158.0
51	249047.0
52	233934.0
53	217351.5
54	201002.5
55	184172.0
56	166493.5
57	148388.0
58	129920.0
59	111014.0
60	91136.0
61	72785.0
62	56809.5
63	42869.0
64	31437.0
65	22504.5
66	16386.0
67	12191.5
68	8745.0
69	6280.5
70	4565.0
71	3307.5
72	2305.5
73	1472.5
74	972.5
75	639.5
76	380.0
77	194.5
78	113.5
79	69.0
80	43.0
81	30.5
82	21.0
83	17.0
84	13.5
85	10.5
86	9.0
87	7.5
88	5.5
89	3.5
90	1.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.004532380526227153
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.406830771915461E-6
26-27	0.0
28-29	5.18660016654914E-5
30-31	0.0
32-33	5.1950353570395654E-5
34-35	0.0
36-37	5.885698103769626E-4
38-39	2.9908802826142597E-5
40-41	1.8017077937750245E-4
42-43	6.0311152776065825E-5
44-45	6.364304615621004E-4
46-47	0.0
48-49	9.200418465699881E-5
50-51	7.710253009639434E-6
52-53	8.523884777535132E-5
54-55	0.002250774784437691
56-57	0.0013704237342355263
58-59	1.258647597316469E-4
60-61	3.399146071266971E-4
62-63	1.3523953188347538E-4
64-65	7.686672031323189E-4
66-67	0.0013467550783150175
68-69	3.4946675031112697E-4
70-71	7.045288672101534E-4
72-73	2.3123002647418642E-4
74-75	1.5805171152534709E-4
76-77	8.380853470917894E-6
78-79	0.0015038155517845644
80-81	0.0
82-83	8.9302090424549E-4
84-85	1.7309725867602408E-4
86-87	8.725302268465161E-6
88-89	3.520001661440784E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.581047553740505E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	312.0
22-23	391.0
24-25	598.0
26-27	1443.0
28-29	2192.0
30-31	7061.0
32-33	9395.0
34-35	13546.0
36-37	23070.0
38-39	26327.0
40-41	27330.0
42-43	31235.0
44-45	38070.0
46-47	39521.0
48-49	37259.0
50-51	33350.0
52-53	31626.0
54-55	34725.0
56-57	37071.0
58-59	21544.0
60-61	40334.0
62-63	39984.0
64-65	43412.0
66-67	47227.0
68-69	47824.0
70-71	50896.0
72-73	44023.0
74-75	44077.0
76-77	47036.0
78-79	48496.0
80-81	47365.0
82-83	46044.0
84-85	46285.0
86-87	48085.0
88-89	50310.0
90-91	51649.0
92-93	52050.0
94-95	63371.0
96-97	234136.0
98-99	488303.0
100-101	4754359.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.31568393384367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.20503032693881	77.86108886942833
2	6.953509635867744	12.00393879920993
3	1.6365749063231132	4.237862470447369
4	0.596070201798646	2.0580082856333735
5	0.2450728052937547	1.057681340125807
6	0.13449369324156393	0.6965349070160495
7	0.07190657551130288	0.4344665671219077
8	0.04386191270385879	0.3028776794944094
9	0.03033277842920297	0.23563750641573553
>10	0.08204613005909696	1.0394531047544664
>50	9.94268788811719E-4	0.05391788152012538
>100	1.0676504411401673E-4	0.018532588832490114
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4811701066101808E-5	0.0	0.0	0.0
3	0.0	1.4811701066101808E-5	0.0	0.0	0.0
4	0.0	1.4811701066101807E-4	0.0	0.0	0.0
5	0.0	1.4811701066101807E-4	0.0	0.0	0.0
6	0.0	1.4811701066101807E-4	0.0	1.4811701066101808E-5	0.0
7	0.0	1.4811701066101807E-4	0.0	1.4811701066101808E-5	0.0
8	0.0	1.6292871172711988E-4	0.0	1.4811701066101808E-5	1.4811701066101808E-5
9	0.0	2.3698721705762892E-4	0.0	1.4811701066101808E-5	2.9623402132203615E-5
10-11	0.0	2.3698721705762892E-4	0.0	2.221755159915271E-5	2.9623402132203615E-5
12-13	0.0	2.4439306759067983E-4	0.0	2.9623402132203615E-5	2.9623402132203615E-5
14-15	0.0	3.1104572238813794E-4	0.0	2.9623402132203615E-5	2.9623402132203615E-5
16-17	0.0	3.2585742345423975E-4	0.0	5.924680426440723E-5	2.9623402132203615E-5
18-19	0.0	3.3326327398729063E-4	0.0	8.887020639661084E-5	2.9623402132203615E-5
20-21	0.0	3.4066912452034156E-4	0.0	1.1108775799576356E-4	2.9623402132203615E-5
22-23	0.0	3.702925266525452E-4	0.0	1.3330530959491626E-4	2.9623402132203615E-5
24-25	0.0	4.221334803839015E-4	0.0	1.5552286119406897E-4	7.405850533050903E-5
26-27	0.0	4.5916273304915604E-4	0.0	2.2217551599152711E-4	7.405850533050903E-5
28-29	0.0	4.6656858358220697E-4	0.0	2.9623402132203613E-4	7.405850533050903E-5
30-31	0.0	5.702504910449196E-4	0.0	7.405850533050904E-4	7.405850533050903E-5
32-33	0.0	5.776563415779705E-4	0.0	0.0017551865763330641	7.405850533050903E-5
34-35	0.0	6.591206974415304E-4	0.0	0.0036436784622610446	8.887020639661084E-5
36-37	0.0	7.331792027720394E-4	0.0	0.0071910808675924275	9.627605692966175E-5
38-39	0.0	7.628026049042432E-4	0.0	0.018788642802350142	1.1108775799576356E-4
40-41	0.0	8.590786618339048E-4	0.0	0.03199327430277991	1.4811701066101807E-4
42-43	0.0	9.035137650322102E-4	0.0	0.03928063122730199	1.5552286119406897E-4
44-45	0.0	0.0010071956724949228	0.0	0.04761221307698427	1.7774041279322169E-4
46-47	0.0	0.001081254177825432	0.0	0.057269442172082644	1.925521138593235E-4
48-49	0.0	0.0010886600283584829	0.0	0.06639345002880136	1.925521138593235E-4
50-51	0.0	0.0012441828895525518	0.0	0.07590256211323872	1.925521138593235E-4
52-53	0.0	0.0012812121422178064	0.0	0.10790324226655167	2.073638149254253E-4
54-55	0.0	0.001318241394883061	0.0	0.14849470903820366	2.2217551599152711E-4
56-57	0.0	0.0014071116012796716	0.0	0.1960698928625227	2.2217551599152711E-4
58-59	0.0	0.0014071116012796716	0.0	0.3592429976572332	2.2217551599152711E-4
60-61	0.0	0.001458952555011028	0.0	0.49739913935129787	2.2217551599152711E-4
62-63	0.0	0.0014885759571432317	0.0	0.5037533591086556	2.2217551599152711E-4
64-65	0.0	0.0015996637151389952	0.0	0.5093151528589768	2.2217551599152711E-4
66-67	0.0	0.001614475416205097	0.0	0.5139956503958649	2.2217551599152711E-4
68-69	0.0	0.0016366929678042499	0.0	0.5184539724167616	2.2217551599152711E-4
70-71	0.0	0.0016440988183373006	0.0	0.5227863949785962	2.2958136652457802E-4
72-73	0.0	0.0016440988183373006	0.0	0.5263337973839277	2.666106191898325E-4
74-75	0.0	0.0016663163699364534	0.0	0.5290295269779584	2.666106191898325E-4
76-77	0.0	0.001769998277399166	0.0	0.5313327464937371	2.666106191898325E-4
78-79	0.0	0.0018218392311305223	0.0	0.5332582676323303	2.666106191898325E-4
80-81	0.0	0.0018366509321966241	0.0	0.5337396479169787	2.740164697228834E-4
82-83	0.0	0.001844056782729675	0.0	0.5340803170414989	3.184515729211889E-4
84-85	0.0	0.0018662743343288279	0.0	0.5342728691553583	3.4066912452034156E-4
86-87	0.0	0.0018958977364610314	0.0	0.5344209861660193	3.4066912452034156E-4
88	0.0	0.0019403328396593368	0.0	0.5344357978670854	3.4066912452034156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8420	0.0	38.195465	1
GTATCAA	13760	0.0	30.80198	1
TCAACGC	15810	0.0	26.641537	4
ATCAACG	15815	0.0	26.633114	3
CAACGCA	16005	0.0	26.344358	5
AACGCAG	16585	0.0	25.42099	6
TATCAAC	17080	0.0	24.840397	2
ACGCAGA	19195	0.0	22.032993	7
CGCAGAG	19765	0.0	21.353191	8
AGAGTAC	19655	0.0	18.146883	10-11
GAGTACT	11825	0.0	18.123745	12-13
GCAGAGT	24045	0.0	17.47935	9
CGAGACC	6240	0.0	17.42197	86-87
AGACCGA	6575	0.0	16.863033	88-89
GTACATG	17135	0.0	16.515646	1
TACATGG	17520	0.0	16.453232	2
ACCGAGG	6750	0.0	16.34407	90-91
GAGACCG	6495	0.0	16.328531	88-89
GACCGAG	6485	0.0	16.25772	90-91
CAGAGTA	22920	0.0	16.250908	10-11
>>END_MODULE