FastQCFastQC Report
Fri 27 May 2016
SRR2138987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698447
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124950.039.6035651
ATCTCGT83650.033.40172294
GTATCAA198050.030.0723691
TATCTCG95000.028.56144194
TCAACGC222450.026.5950454
ATCAACG223250.026.4403723
CAACGCA227100.026.0894095
GCCGTCT45500.025.65205490-91
AACGCAG234350.025.4693766
TATCAAC245150.024.3126662
ATGCCGT48850.023.97656288-89
TATGCCG45800.023.60843886-87
TGCCGTC47100.023.1757588-89
CCGTCTT48050.023.11263590-91
ACGCAGA275450.021.6370037
CGCAGAG277400.021.4371268
TCGTATG54900.021.08009184-85
TCTCGTA52750.020.81920682-83
CGTATGC52350.020.61320584-85
CTCGTAT53700.019.68097982-83
Produced by FastQC (version 0.11.5)