##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9698447
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.7174708486833	25.0	18.0	32.0	18.0	33.0
2	26.81551922694427	28.0	18.0	32.0	18.0	33.0
3	29.025075870394506	31.0	27.0	33.0	18.0	33.0
4	29.708932058916236	31.0	29.0	33.0	25.0	33.0
5	31.11985465301816	32.0	32.0	33.0	28.0	33.0
6	36.085708361348985	37.0	36.0	38.0	33.0	38.0
7	36.201392449739636	38.0	37.0	38.0	33.0	38.0
8	36.86237095485494	38.0	38.0	38.0	35.0	38.0
9	37.01272708919274	38.0	38.0	38.0	36.0	38.0
10-11	37.084405884777226	38.0	38.0	38.0	36.0	38.0
12-13	37.12997287091428	38.0	38.0	38.0	36.0	38.0
14-15	37.151787136641566	38.0	38.0	38.0	36.0	38.0
16-17	37.127161802296804	38.0	38.0	38.0	36.0	38.0
18-19	37.16385252195532	38.0	38.0	38.0	36.0	38.0
20-21	37.13025372482201	38.0	38.0	38.0	36.0	38.0
22-23	37.17156619680358	38.0	38.0	38.0	36.0	38.0
24-25	37.17915977092325	38.0	38.0	38.0	36.0	38.0
26-27	37.112354783143005	38.0	38.0	38.0	36.0	38.0
28-29	37.0995441851273	38.0	38.0	38.0	36.0	38.0
30-31	36.968268972431986	38.0	38.0	38.0	36.0	38.0
32-33	37.06498879992895	38.0	38.0	38.0	36.0	38.0
34-35	37.08566861321512	38.0	38.0	38.0	36.0	38.0
36-37	37.01196977778367	38.0	38.0	38.0	36.0	38.0
38-39	37.05329421287341	38.0	38.0	38.0	36.0	38.0
40-41	36.96838725089103	38.0	38.0	38.0	36.0	38.0
42-43	36.94992889896386	38.0	38.0	38.0	36.0	38.0
44-45	36.95035438598276	38.0	38.0	38.0	36.0	38.0
46-47	36.912068436961846	38.0	38.0	38.0	35.5	38.0
48-49	36.87871212808065	38.0	38.0	38.0	35.0	38.0
50-51	36.896579375189006	38.0	38.0	38.0	35.0	38.0
52-53	36.805146327108076	38.0	38.0	38.0	35.0	38.0
54-55	36.794027455160354	38.0	38.0	38.0	35.0	38.0
56-57	36.801913112685966	38.0	38.0	38.0	35.0	38.0
58-59	36.822318758155006	38.0	38.0	38.0	35.0	38.0
60-61	36.81720972251547	38.0	38.0	38.0	35.0	38.0
62-63	36.819199726479056	38.0	38.0	38.0	35.0	38.0
64-65	36.77254424757216	38.0	38.0	38.0	35.0	38.0
66-67	36.7739090766644	38.0	38.0	38.0	35.0	38.0
68-69	36.788758025993005	38.0	38.0	38.0	35.0	38.0
70-71	36.70538826195818	38.0	38.0	38.0	34.5	38.0
72-73	36.65529731125245	38.0	38.0	38.0	34.0	38.0
74-75	36.70175394286228	38.0	38.0	38.0	34.5	38.0
76-77	36.673868283135754	38.0	38.0	38.0	34.0	38.0
78-79	36.687897236760854	38.0	38.0	38.0	34.0	38.0
80-81	36.7102852320311	38.0	38.0	38.0	34.0	38.0
82-83	36.5675426489119	38.0	38.0	38.0	34.0	38.0
84-85	36.59950787390038	38.0	38.0	38.0	34.0	38.0
86-87	36.59794440989194	38.0	38.0	38.0	34.0	38.0
88-89	36.54038511086335	38.0	38.0	38.0	34.0	38.0
90-91	36.544513415766275	38.0	38.0	38.0	34.0	38.0
92-93	36.589355737137794	38.0	38.0	38.0	34.0	38.0
94-95	36.466114876752464	38.0	38.0	38.0	34.0	38.0
96-97	36.39377820883734	38.0	38.0	38.0	34.0	38.0
98-99	36.473136622809896	38.0	38.0	38.0	34.0	38.0
100	34.85239334843072	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	47.0
19	51.0
20	99.0
21	327.0
22	1060.0
23	2956.0
24	7187.0
25	13954.0
26	24083.0
27	38465.0
28	57301.0
29	81216.0
30	110028.0
31	146892.0
32	196629.0
33	271258.0
34	413219.0
35	769672.0
36	2233014.0
37	5330983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.22913575750839	23.7154464008516	14.853398693625897	24.20201914801411
2	13.605714399429106	21.57997048393418	40.37971233951168	24.43460277712504
3	16.299011584019585	26.598536858530032	32.54840697691084	24.554044580539543
4	12.302516062623223	19.024355136446072	40.2518155741842	28.42131322674651
5	13.016532291742926	37.46113369883863	36.22629019002907	13.296043819389377
6	28.17757898302254	39.60158561501354	19.57282842765	12.648006974313924
7	24.79710411367923	33.357144705745156	24.981040779003074	16.86471040157254
8	23.36574092738765	37.420826241562175	22.193852273461925	17.01958055758824
9	25.066008004375963	16.840328526892716	22.244504144736606	35.84915932399472
10-11	23.216505694159075	27.369660317780774	30.033777572842336	19.380056415217815
12-13	24.08733583840794	24.980344791284626	30.07092785061361	20.86139151969382
14-15	21.540309494911916	26.351342642796315	27.40210365638952	24.706244205902244
16-17	21.125160554055718	29.29692764212662	28.17256721617389	21.405344587643775
18-19	21.132739086989908	28.777488808259715	29.522432818367722	20.567339286382655
20-21	22.07403645756134	27.868909508275326	29.151283541768436	20.9057704923949
22-23	21.79644755529875	27.794513817849726	28.98295340268136	21.426085224170162
24-25	21.56485710795752	27.90737448552796	29.085431220161162	21.44233718635336
26-27	21.510239449913698	28.096628130703248	29.021333459293018	21.37179896009003
28-29	21.423896544419506	28.12782410215827	29.077668898878105	21.370610454544117
30-31	21.698648740562955	27.945936204981976	29.006282812972035	21.349132241483037
32-33	21.57790832248331	28.055448777564223	28.913070659474656	21.453572240477815
34-35	21.643083606036058	28.064824544357673	28.92601745694178	21.366074392664494
36-37	21.609192575701428	28.02488587179073	28.990840119443632	21.375081433064214
38-39	21.467965914491096	28.098222727588944	29.009183652072185	21.424627705847776
40-41	21.75689549445136	27.935683656870715	28.97417681575781	21.333244032920113
42-43	21.57089808528197	27.99679603713539	28.81577470424343	21.616531173339204
44-45	21.632156699453784	28.015772130776867	28.731886686974118	21.620184482795235
46-47	21.673394214888127	27.992451334656366	28.704017847581802	21.630136602873705
48-49	21.574888362459365	28.169432323819187	28.6912545957772	21.56442471794425
50-51	21.56707782290559	28.230571091054635	28.513886747217356	21.68846433882242
52-53	21.65783678834426	28.094073396845705	28.384770571456574	21.863319243353466
54-55	21.7316182960911	27.860460255679442	28.66113303067633	21.74678841755313
56-57	21.60874150503001	27.67749573601058	28.731783354648126	21.981979404311282
58-59	21.478871439551916	27.55350777767883	28.80982775546727	22.15779302730198
60-61	21.665535075653665	27.743219659307204	28.998169142153802	21.593076122885325
62-63	21.330386807434724	27.92118973737458	28.9783160333497	21.77010742184099
64-65	21.21298841041512	28.164904528861847	28.696533596640293	21.92557346408274
66-67	21.250208574026612	28.580033542840482	28.336821748293	21.832936134839905
68-69	21.293945792026754	28.564382020979412	28.186948929484483	21.954723257509354
70-71	21.383293466579474	28.15185951130686	28.151730692068476	22.313116330045194
72-73	21.513295102004143	27.8300262827219	28.147650561256444	22.50902805401752
74-75	21.534671197930773	27.869133481024704	28.249966397597277	22.346228923447246
76-77	21.827642261889725	27.76194847701539	27.774650495398767	22.635758765696117
78-79	21.977115571337638	27.856107724968926	27.440025103574484	22.72675160011896
80-81	21.87442799769682	28.0631558680688	27.528255275844955	22.534160858389424
82-83	21.827320404416074	27.944102036613632	27.607053411452465	22.62152414751783
84-85	21.936424571468283	28.16846265596788	27.58344066333195	22.311672109231885
86-87	22.201546643629815	28.088424416860253	27.6503835990623	22.059645340447638
88-89	22.03829502905337	28.19882823296228	27.884572259759977	21.878304478224376
90-91	22.035378794897458	27.905962330863264	27.803893277360697	22.254765596878574
92-93	22.043670104754025	27.585943446280492	27.941537247090935	22.42884920187455
94-95	21.525651380382268	27.92187125777794	28.352281747727144	22.20019561411265
96-97	21.631172847361775	27.883705886933217	28.534011655328516	21.951109610376488
98-99	22.747807794103977	29.736655874310348	28.86972352867253	18.645812802913145
100	21.70121355894144	30.782562924903335	22.734249169364077	24.78197434679115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	854.0
1	994.5
2	1706.0
3	2484.0
4	3096.5
5	4127.0
6	5023.5
7	5992.0
8	6999.5
9	7717.5
10	8066.0
11	8415.5
12	9642.0
13	11896.0
14	15851.0
15	20397.0
16	23432.5
17	24817.0
18	25047.5
19	24277.5
20	23418.0
21	23759.0
22	26838.0
23	33591.0
24	44179.0
25	58416.0
26	76788.5
27	98551.5
28	121206.0
29	146193.0
30	171740.0
31	196729.5
32	223568.0
33	248122.5
34	270176.0
35	291962.0
36	315392.0
37	336974.0
38	352632.0
39	365419.5
40	379049.5
41	390895.5
42	399490.0
43	406789.5
44	410179.5
45	413445.5
46	413154.5
47	407838.0
48	400118.5
49	386231.0
50	369656.0
51	349882.0
52	326893.0
53	301955.0
54	275279.5
55	248334.5
56	221516.0
57	195001.5
58	168592.0
59	142081.5
60	115082.0
61	89732.5
62	68324.5
63	51036.0
64	36615.5
65	25788.5
66	18208.5
67	13311.0
68	9918.5
69	7031.5
70	4906.5
71	3396.5
72	2312.0
73	1555.0
74	981.0
75	623.5
76	407.0
77	241.0
78	140.5
79	75.5
80	46.5
81	33.5
82	24.0
83	16.5
84	13.0
85	11.0
86	8.5
87	5.5
88	3.5
89	3.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.004712094627108855
6	2.0621858324327596E-5
7	0.0
8	0.0
9	2.0621858324327596E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.156128073683958E-6
26-27	0.0
28-29	4.126304744007407E-5
30-31	0.0
32-33	4.6500071377609564E-5
34-35	0.0
36-37	5.447431816052368E-4
38-39	5.203288040965695E-5
40-41	2.505695105662292E-4
42-43	9.436980812049053E-5
44-45	7.95682364021836E-4
46-47	0.0
48-49	8.533036156127469E-5
50-51	1.6090468767917407E-5
52-53	1.343231480746684E-4
54-55	0.0022577275563604054
56-57	0.0011778168997041046
58-59	1.5065959578138065E-4
60-61	4.055219326807638E-4
62-63	1.1427379249740313E-4
64-65	8.160725767847616E-4
66-67	0.0013844986675165612
68-69	3.390272496764513E-4
70-71	7.449056991390851E-4
72-73	1.7496198104371594E-4
74-75	1.8194161266222653E-4
76-77	1.145874328181043E-5
78-79	0.0015420925421863383
80-81	0.0
82-83	9.042843760622773E-4
84-85	1.1838941617561697E-4
86-87	1.1937016718687191E-5
88-89	9.6321731922533E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.702320539207215E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	97.0
20-21	416.0
22-23	592.0
24-25	813.0
26-27	1957.0
28-29	3302.0
30-31	10708.0
32-33	14199.0
34-35	21027.0
36-37	34935.0
38-39	22066.0
40-41	40597.0
42-43	45663.0
44-45	55518.0
46-47	57549.0
48-49	54100.0
50-51	27802.0
52-53	4625.0
54-55	4488.0
56-57	4458.0
58-59	31119.0
60-61	59523.0
62-63	58453.0
64-65	63228.0
66-67	67710.0
68-69	68560.0
70-71	69737.0
72-73	64823.0
74-75	66423.0
76-77	69050.0
78-79	71496.0
80-81	71160.0
82-83	68427.0
84-85	68865.0
86-87	71288.0
88-89	74070.0
90-91	76325.0
92-93	76634.0
94-95	94570.0
96-97	330448.0
98-99	760054.0
100-101	6911572.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.56816834034562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.07020652320686	75.32504219363908
2	7.619139703632966	12.886733781308873
3	1.8857320583864596	4.784187184752377
4	0.6709329966122839	2.2695829841040065
5	0.3074825959379871	1.3001619967505083
6	0.15711562481352878	0.7972188364877453
7	0.09027449583251283	0.5344044132282643
8	0.05319033239332639	0.3598567187134212
9	0.03400458857033767	0.2588135193504457
>10	0.11059311688424406	1.37126180888043
>50	0.0011038234558822298	0.06295842520188417
>100	2.0493718831171464E-4	0.03683509871044886
>500	1.920308524456332E-5	0.012943038872561416
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0310929162163798E-5	0.0	0.0	0.0
3	0.0	1.0310929162163798E-5	0.0	0.0	0.0
4	0.0	3.0932787486491395E-5	0.0	0.0	0.0
5	0.0	5.155464581081899E-5	0.0	0.0	0.0
6	3.0932787486491395E-5	5.155464581081899E-5	0.0	0.0	0.0
7	3.0932787486491395E-5	5.155464581081899E-5	0.0	0.0	0.0
8	3.0932787486491395E-5	5.155464581081899E-5	0.0	0.0	1.0310929162163798E-5
9	3.0932787486491395E-5	1.2373114994596558E-4	0.0	0.0	1.0310929162163798E-5
10-11	3.6088252067573294E-5	1.2373114994596558E-4	0.0	5.155464581081899E-6	1.0310929162163798E-5
12-13	4.124371664865519E-5	1.2373114994596558E-4	0.0	2.5777322905409497E-5	1.0310929162163798E-5
14-15	4.124371664865519E-5	1.5466393743245697E-4	0.0	3.0932787486491395E-5	1.5466393743245698E-5
16-17	6.186557497298279E-5	1.5466393743245697E-4	0.0	3.0932787486491395E-5	2.0621858324327596E-5
18-19	6.186557497298279E-5	1.7528579575678456E-4	0.0	6.186557497298279E-5	2.0621858324327596E-5
20-21	6.186557497298279E-5	2.2684044156760354E-4	0.0	1.1857568536488368E-4	3.0932787486491395E-5
22-23	7.217650413514659E-5	3.2994973318924153E-4	0.0	1.7013033117570266E-4	4.124371664865519E-5
24-25	8.248743329731038E-5	3.608825206757329E-4	0.0	2.2168497698652165E-4	6.186557497298279E-5
26-27	8.248743329731038E-5	3.711934498378967E-4	0.0	2.4746229989193116E-4	6.186557497298279E-5
28-29	8.248743329731038E-5	3.711934498378967E-4	0.0	4.7430274145953473E-4	6.186557497298279E-5
30-31	8.248743329731038E-5	4.330590248108795E-4	0.0	0.0012527778932029015	6.186557497298279E-5
32-33	8.248743329731038E-5	4.382144893919614E-4	0.0	0.0032324762923383508	6.186557497298279E-5
34-35	9.279836245947418E-5	4.8461367062169853E-4	0.0	0.0062896667889199166	6.186557497298279E-5
36-37	9.279836245947418E-5	5.310128518514356E-4	5.155464581081899E-6	0.014512632795745545	6.702103955406469E-5
38-39	9.279836245947418E-5	5.722565685000908E-4	1.0310929162163798E-5	0.12444775952273596	7.733196871622849E-5
40-41	9.279836245947418E-5	5.877229622433365E-4	1.0310929162163798E-5	0.24205421754637624	8.248743329731038E-5
42-43	9.279836245947418E-5	6.031893559865822E-4	1.0310929162163798E-5	0.26427942535542026	8.248743329731038E-5
44-45	9.279836245947418E-5	6.547440017974012E-4	1.0310929162163798E-5	0.2955215407167766	9.279836245947418E-5
46-47	9.279836245947418E-5	6.908322538649745E-4	1.0310929162163798E-5	0.3312746875865796	9.279836245947418E-5
48-49	9.279836245947418E-5	6.908322538649745E-4	1.0310929162163798E-5	0.3641046860389091	1.1857568536488368E-4
50-51	9.279836245947418E-5	7.836306163244486E-4	1.0310929162163798E-5	0.39943508481306333	1.2373114994596558E-4
52-53	9.795382704055608E-5	7.939415454866124E-4	1.0310929162163798E-5	0.7677620963438786	1.3919754368921128E-4
54-55	1.0310929162163798E-4	8.042524746487762E-4	1.0310929162163798E-5	1.2695898632018094	1.5466393743245697E-4
56-57	1.0310929162163798E-4	8.248743329731038E-4	1.0310929162163798E-5	1.824462205134492	1.5981940201353887E-4
58-59	1.0310929162163798E-4	8.351852621352676E-4	1.0310929162163798E-5	2.4066997530635574	1.7013033117570266E-4
60-61	1.0310929162163798E-4	8.506516558785133E-4	1.0310929162163798E-5	2.5638383134949336	1.7528579575678456E-4
62-63	1.0310929162163798E-4	8.970508371082504E-4	1.0310929162163798E-5	2.5711745395938133	1.7528579575678456E-4
64-65	1.0310929162163798E-4	0.0010104710578920522	1.0310929162163798E-5	2.5767939959871926	1.7528579575678456E-4
66-67	1.0310929162163798E-4	0.001025937451635298	1.0310929162163798E-5	2.581346271212288	1.7528579575678456E-4
68-69	1.0310929162163798E-4	0.0010671811682839531	1.0310929162163798E-5	2.585553130310451	1.7528579575678456E-4
70-71	1.0310929162163798E-4	0.0010929584911893626	1.0310929162163798E-5	2.5892495984150865	1.8559672491894836E-4
72-73	1.1342022078380177E-4	0.0011084248849326083	1.0310929162163798E-5	2.592306788911668	2.2684044156760354E-4
74-75	1.1342022078380177E-4	0.0011393576724190997	1.0310929162163798E-5	2.594925764918858	2.2684044156760354E-4
76-77	1.1342022078380177E-4	0.0011909123182299187	1.0310929162163798E-5	2.5975705182489524	2.2684044156760354E-4
78-79	1.1342022078380177E-4	0.0012166896411353282	1.0310929162163798E-5	2.599117157623277	2.3715137072976737E-4
80-81	1.1342022078380177E-4	0.0012166896411353282	1.0310929162163798E-5	2.5996894141917775	2.4746229989193116E-4
82-83	1.1342022078380177E-4	0.0012166896411353282	1.0310929162163798E-5	2.6001946497207236	2.5777322905409496E-4
84-85	1.1342022078380177E-4	0.0012166896411353282	1.0310929162163798E-5	2.6003905573748045	2.5777322905409496E-4
86-87	1.1342022078380177E-4	0.001227000570297492	1.0310929162163798E-5	2.6005039775955883	2.5777322905409496E-4
88	1.1342022078380177E-4	0.0012682442869461472	1.0310929162163798E-5	2.6005194439893313	2.5777322905409496E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12495	0.0	39.603565	1
ATCTCGT	8365	0.0	33.401722	94
GTATCAA	19805	0.0	30.072369	1
TATCTCG	9500	0.0	28.561441	94
TCAACGC	22245	0.0	26.595045	4
ATCAACG	22325	0.0	26.440372	3
CAACGCA	22710	0.0	26.089409	5
GCCGTCT	4550	0.0	25.652054	90-91
AACGCAG	23435	0.0	25.469376	6
TATCAAC	24515	0.0	24.312666	2
ATGCCGT	4885	0.0	23.976562	88-89
TATGCCG	4580	0.0	23.608438	86-87
TGCCGTC	4710	0.0	23.17575	88-89
CCGTCTT	4805	0.0	23.112635	90-91
ACGCAGA	27545	0.0	21.637003	7
CGCAGAG	27740	0.0	21.437126	8
TCGTATG	5490	0.0	21.080091	84-85
TCTCGTA	5275	0.0	20.819206	82-83
CGTATGC	5235	0.0	20.613205	84-85
CTCGTAT	5370	0.0	19.680979	82-83
>>END_MODULE