FastQCFastQC Report
Fri 27 May 2016
SRR2138986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22328808
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA253950.050.653211
GTATCAA467250.038.3443261
TCAACGC543000.031.976374
ATCAACG543900.031.8994333
CAACGCA557150.031.1408085
TATCAAC578300.030.3333342
AACGCAG576350.030.1932986
ACGCAGA649200.026.7514787
CGCAGAG666950.026.0525828
GCAGAGT802200.021.7035889
GTACATG638300.021.6334691
TACATGG644200.020.650952
ACATGGG632600.020.2859463
GAGTACT453600.019.71496212-13
AGAGTAC723700.019.20650310-11
CAGAGTA793200.018.86632310-11
CATGGGG432100.018.1862854
GTGGTAT127100.016.930631
GTACTTT526150.016.8476314-15
AGTACTT511250.016.29476712-13
Produced by FastQC (version 0.11.5)