##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22328808
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.516932296609834	30.0	18.0	33.0	18.0	33.0
2	30.670855381084383	32.0	29.0	33.0	27.0	33.0
3	31.807325317141874	33.0	31.0	33.0	29.0	33.0
4	32.347397138261925	33.0	33.0	33.0	31.0	34.0
5	32.77845024239539	33.0	33.0	34.0	32.0	34.0
6	36.25532885588877	38.0	37.0	38.0	33.0	38.0
7	36.68244592366955	38.0	37.0	38.0	34.0	38.0
8	36.54303194330839	38.0	37.0	38.0	34.0	38.0
9	36.895217380166464	38.0	38.0	38.0	35.0	38.0
10-11	37.05226716535876	38.0	38.0	38.0	35.5	38.0
12-13	37.11163999439648	38.0	38.0	38.0	36.0	38.0
14-15	37.10159848210437	38.0	38.0	38.0	36.0	38.0
16-17	37.07486172123474	38.0	38.0	38.0	36.0	38.0
18-19	37.111723989027986	38.0	38.0	38.0	36.0	38.0
20-21	37.0908481840813	38.0	38.0	38.0	36.0	38.0
22-23	37.13226625553742	38.0	38.0	38.0	36.0	38.0
24-25	37.142192268587024	38.0	38.0	38.0	36.0	38.0
26-27	37.07549564987005	38.0	38.0	38.0	36.0	38.0
28-29	37.0646630896571	38.0	38.0	38.0	36.0	38.0
30-31	36.93527724711264	38.0	38.0	38.0	36.0	38.0
32-33	37.03382765842645	38.0	38.0	38.0	36.0	38.0
34-35	37.047554649279405	38.0	38.0	38.0	36.0	38.0
36-37	36.97032025371752	38.0	38.0	38.0	36.0	38.0
38-39	37.00888960362773	38.0	38.0	38.0	36.0	38.0
40-41	36.92707715324667	38.0	38.0	38.0	35.5	38.0
42-43	36.91186956435425	38.0	38.0	38.0	35.5	38.0
44-45	36.91058577254738	38.0	38.0	38.0	35.5	38.0
46-47	36.86961835419126	38.0	38.0	38.0	35.0	38.0
48-49	36.832377867254166	38.0	38.0	38.0	35.0	38.0
50-51	36.845082248500205	38.0	38.0	38.0	35.0	38.0
52-53	36.75644330532464	38.0	38.0	38.0	35.0	38.0
54-55	36.742091473391284	38.0	38.0	38.0	35.0	38.0
56-57	36.74366878991731	38.0	38.0	38.0	35.0	38.0
58-59	36.763749695799106	38.0	38.0	38.0	35.0	38.0
60-61	36.7610951997394	38.0	38.0	38.0	35.0	38.0
62-63	36.7682524808597	38.0	38.0	38.0	35.0	38.0
64-65	36.7229496533468	38.0	38.0	38.0	35.0	38.0
66-67	36.72911371953881	38.0	38.0	38.0	35.0	38.0
68-69	36.74270982786727	38.0	38.0	38.0	35.0	38.0
70-71	36.65424801059524	38.0	38.0	38.0	34.0	38.0
72-73	36.61295173358789	38.0	38.0	38.0	34.0	38.0
74-75	36.66380703198564	38.0	38.0	38.0	34.0	38.0
76-77	36.641331676235104	38.0	38.0	38.0	34.0	38.0
78-79	36.658981742639156	38.0	38.0	38.0	34.0	38.0
80-81	36.68128440513535	38.0	38.0	38.0	34.0	38.0
82-83	36.54886702362198	38.0	38.0	38.0	34.0	38.0
84-85	36.56646294561969	38.0	38.0	38.0	34.0	38.0
86-87	36.56480826330137	38.0	38.0	38.0	34.0	38.0
88-89	36.511359584159266	38.0	38.0	38.0	34.0	38.0
90-91	36.509311474099675	38.0	38.0	38.0	34.0	38.0
92-93	36.557362530739326	38.0	38.0	38.0	34.0	38.0
94-95	36.45523082725513	38.0	38.0	38.0	34.0	38.0
96-97	36.41316859393212	38.0	38.0	38.0	34.0	38.0
98-99	36.48349335671374	38.0	38.0	38.0	34.0	38.0
100	34.88278116234614	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	16.0
19	79.0
20	217.0
21	765.0
22	2548.0
23	7538.0
24	17267.0
25	33937.0
26	59196.0
27	93510.0
28	138833.0
29	194528.0
30	262653.0
31	344049.0
32	451761.0
33	605260.0
34	878557.0
35	1518562.0
36	4066427.0
37	1.3653099E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36108277701165	19.08868131249998	14.43871970236835	22.111516208120022
2	14.041510858976439	21.184749315771807	42.91391192937841	21.859827895873348
3	16.68365369078367	27.841754920370132	31.242012560634674	24.23257882821152
4	11.746466716897741	19.50772293800905	40.15503201066532	28.590778334427885
5	13.0057797772825	37.85325066118194	36.317654144562326	12.82331541697323
6	28.096070161566182	39.27540057448661	19.947282447614977	12.681246816332232
7	24.977938813392996	33.4849312153161	24.890061305556483	16.64706866573442
8	23.48299112070828	37.10655311291136	22.315530681261624	17.09492508511874
9	25.251518128509144	17.052186574402	22.341085113007374	35.35521018408148
10-11	23.22896278207059	27.35011425598715	30.045576100614056	19.3753468613282
12-13	24.17305258749146	25.137311405069184	29.977207023321622	20.712428984117736
14-15	21.573590045648654	26.380985048552525	27.558725929301737	24.486698976497088
16-17	21.049910053416195	29.28358065508916	28.30067328269382	21.36583600880083
18-19	21.065934195860343	28.633709421479193	29.761850699777614	20.53850568288285
20-21	21.978254223759823	27.77196036682338	29.54134851293182	20.708436896484976
22-23	21.749680723777864	27.71817432448138	29.29618151684589	21.23596343489486
24-25	21.463377327621604	27.842014263497006	29.40442156985807	21.29018683902332
26-27	21.383901347328283	27.966132556935108	29.388254925300878	21.26171117043573
28-29	21.380767651131517	27.980667033361346	29.417740851043185	21.220824464463956
30-31	21.6153227638953	27.858300269004843	29.316650421958222	21.20972654514163
32-33	21.459055555751295	27.937361611399798	29.27676050406882	21.326822328780086
34-35	21.603671024087586	27.96021670956647	29.25002529027617	21.186086976069777
36-37	21.533361056552643	27.92983760954111	29.222826227956677	21.313975105949574
38-39	21.397714155154585	28.005973869334362	29.34381671528343	21.252495260227626
40-41	21.629236330207743	27.871918575350513	29.211224850030494	21.28762024441125
42-43	21.554238532434457	27.93274650694837	29.052442019092112	21.460572941525058
44-45	21.584500358417934	28.01610850186449	28.94766206949651	21.451729070221067
46-47	21.64672477422736	28.009254543346845	28.75316716930606	21.590853513119733
48-49	21.58709136596387	28.069267090423526	28.789060538347265	21.554581005265334
50-51	21.593081639821516	28.206034263976044	28.666804837937566	21.534079258264878
52-53	21.7589890380039	28.096568702358237	28.583009370181518	21.56143288945634
54-55	21.732806156073806	28.036560703115082	28.596450606297047	21.634182534514064
56-57	21.70310002744184	28.084598108073962	28.581971706893448	21.630330157590745
58-59	21.641076016379603	28.091343421818525	28.67013173270702	21.597448829094848
60-61	21.72007452834873	28.125784266164505	28.62583816861498	21.528303036871787
62-63	21.67408853655131	28.148113320935565	28.63096110962222	21.546837032890906
64-65	21.683893784875778	28.108454439870705	28.553073851930883	21.65457792332263
66-67	21.697193657012598	28.17641541936461	28.44007795913828	21.686312964484518
68-69	21.634841916253766	28.177077928293464	28.50428435400754	21.68379580144523
70-71	21.683594642009794	28.011988686897382	28.519712591301055	21.78470407979177
72-73	21.75241974634479	27.97970588418971	28.45100205015918	21.81687231930632
74-75	21.67696033032141	28.09775450264541	28.38217234357325	21.843112823459933
76-77	21.80532126046582	28.042937218872655	28.268065176918988	21.883676343742536
78-79	21.78644129208259	28.010011177460303	28.27503564656981	21.9285118838873
80-81	21.75974107460314	28.071858072072448	28.25726789785205	21.91113295547236
82-83	21.85267994965791	28.045884443140444	28.27144372632302	21.82999188087862
84-85	21.92660554363583	28.047731593965686	28.20545165706754	21.82021120533094
86-87	21.824584866928475	28.065784733895466	28.305234496985758	21.8043959021903
88-89	21.891549218600545	28.08789979057897	28.230888641635982	21.789662349184503
90-91	21.945802904606644	28.115623846761356	28.2032720349361	21.735301213695905
92-93	21.93362863692724	28.110219486419208	28.244460779108227	21.71169109754533
94-95	21.855717274851948	28.140061764720905	28.233687550750947	21.770533409676204
96-97	21.97044347261422	28.19949329594347	28.303753868073745	21.526309363368572
98-99	22.951970493253484	30.13303524285001	28.587727370609578	18.32726689328693
100	21.821335284831918	31.014686760478067	22.56853645821508	24.59544149647494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2391.0
1	2622.5
2	4628.5
3	6989.5
4	9021.0
5	13047.5
6	16482.0
7	19497.0
8	23219.5
9	25183.0
10	24597.5
11	24234.0
12	26971.0
13	32428.5
14	42412.5
15	54867.5
16	65243.5
17	72940.5
18	75900.0
19	73269.0
20	69512.0
21	68942.5
22	74664.0
23	89242.5
24	113114.5
25	148046.5
26	192361.0
27	243017.0
28	299766.0
29	361398.5
30	423108.5
31	482187.5
32	541778.5
33	598963.5
34	655380.0
35	709107.0
36	760319.5
37	805504.5
38	839200.0
39	867975.5
40	893391.5
41	915525.0
42	926114.0
43	931010.0
44	933824.5
45	935008.0
46	927100.5
47	908611.5
48	889820.5
49	863103.5
50	829571.0
51	786633.5
52	739676.0
53	690639.5
54	633542.5
55	576413.0
56	519655.5
57	459826.0
58	400932.0
59	341696.5
60	280167.5
61	221968.0
62	171545.5
63	128818.0
64	93379.0
65	67075.0
66	48578.5
67	35815.5
68	26852.0
69	19551.5
70	13970.5
71	9936.0
72	6789.5
73	4534.0
74	2963.5
75	1849.5
76	1122.5
77	638.0
78	384.0
79	233.5
80	157.5
81	110.0
82	78.0
83	50.0
84	28.0
85	17.5
86	14.0
87	11.5
88	9.5
89	7.0
90	5.5
91	3.5
92	2.5
93	1.5
94	1.0
95	2.5
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0027408538780932684
6	8.957038817298264E-6
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.1197418323231396E-5
26-27	0.0
28-29	5.1523010097905145E-5
30-31	0.0
32-33	3.815022742697048E-5
34-35	0.0
36-37	5.86047599642574E-4
38-39	4.75244330465264E-5
40-41	1.9776908740500295E-4
42-43	6.166713960033481E-5
44-45	6.477390576315491E-4
46-47	0.0
48-49	9.545427644703911E-5
50-51	4.685889591538033E-6
52-53	9.895232109632386E-5
54-55	0.0022056974183058005
56-57	0.0013323883148734388
58-59	1.4633534202179835E-4
60-61	3.863960014196189E-4
62-63	1.0453555206720147E-4
64-65	7.711419772995876E-4
66-67	0.0014306758964337666
68-69	3.4318582535109213E-4
70-71	6.921305508682101E-4
72-73	2.073238673132306E-4
74-75	1.5034787823340633E-4
76-77	1.7983720262923018E-5
78-79	0.0016662386618678303
80-81	0.0
82-83	0.0010339026980983284
84-85	1.8621302195111027E-4
86-87	2.6836320877809615E-6
88-89	5.6879151467067704E-5
90-91	0.0
92-93	0.0
94-95	5.581434964045373E-6
96-97	2.7774778290666453E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	544.0
22-23	1238.0
24-25	1602.0
26-27	3558.0
28-29	8096.0
30-31	25460.0
32-33	35412.0
34-35	51282.0
36-37	84243.0
38-39	97017.0
40-41	101025.0
42-43	117091.0
44-45	142137.0
46-47	148562.0
48-49	138297.0
50-51	122536.0
52-53	115339.0
54-55	126518.0
56-57	131763.0
58-59	137956.0
60-61	137085.0
62-63	141189.0
64-65	150654.0
66-67	162825.0
68-69	166279.0
70-71	166456.0
72-73	154458.0
74-75	157316.0
76-77	165454.0
78-79	169342.0
80-81	169520.0
82-83	162602.0
84-85	162519.0
86-87	169424.0
88-89	175835.0
90-91	179198.0
92-93	182065.0
94-95	220184.0
96-97	708461.0
98-99	1579239.0
100-101	1.5458889E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.45265418298659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.68344227061398	73.12185197295791
2	7.317827355299834	12.067485765946733
3	2.1232336384253743	5.251987468163153
4	0.8444437306948271	2.7850650761588662
5	0.3893259840881838	1.605048036523698
6	0.2165480834497928	1.0712978543204572
7	0.12663739501091162	0.7309112536228277
8	0.07802610863647255	0.5146767802117772
9	0.05120195416003891	0.37995633178657384
>10	0.16470474012932626	2.0822680642969735
>50	0.0033748519113421115	0.18198790957156571
>100	0.0011600907381434412	0.15408657602919343
>500	5.277971695643462E-5	0.027825463434991284
>1k	2.1017124780297808E-5	0.02555144697530195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7914077634596527E-5	0.0	8.957038817298264E-6	0.0
3	0.0	2.2392597043245658E-5	0.0	1.3435558225947395E-5	0.0
4	0.0	4.030667467784218E-5	0.0	2.2392597043245658E-5	0.0
5	0.0	4.926371349514045E-5	0.0	2.687111645189479E-5	0.0
6	4.030667467784218E-5	5.374223290378958E-5	0.0	2.687111645189479E-5	0.0
7	4.030667467784218E-5	5.374223290378958E-5	0.0	5.822075231243871E-5	4.478519408649132E-6
8	4.4785194086491317E-5	6.269927172108784E-5	0.0	7.165631053838611E-5	8.957038817298264E-6
9	4.4785194086491317E-5	1.1644150462487743E-4	0.0	8.957038817298263E-5	8.957038817298264E-6
10-11	4.4785194086491317E-5	1.1644150462487743E-4	0.0	9.404890758163176E-5	8.957038817298264E-6
12-13	4.4785194086491317E-5	1.231592837378511E-4	0.0	9.404890758163176E-5	8.957038817298264E-6
14-15	4.4785194086491317E-5	1.70183737528667E-4	0.0	1.052452061032546E-4	1.3435558225947395E-5
16-17	7.165631053838611E-5	1.836192957546144E-4	0.0	1.1644150462487743E-4	2.2392597043245658E-5
18-19	7.83740896513598E-5	2.0153337338921093E-4	0.0	1.2539854344217568E-4	3.3588895564868484E-5
20-21	9.852742699028089E-5	2.2392597043245657E-4	0.0	1.567481793027196E-4	3.5828155269193055E-5
22-23	1.1420224492055285E-4	2.463185674757022E-4	0.0	1.6794447782434243E-4	4.926371349514045E-5
24-25	1.3435558225947394E-4	2.6871116451894787E-4	0.0	1.8585855545893894E-4	5.374223290378958E-5
26-27	1.5003040018974592E-4	2.8662524215354444E-4	0.0	2.2616523013678115E-4	5.598149260811415E-5
28-29	1.567481793027196E-4	2.8886450185786896E-4	0.0	3.806741497351762E-4	5.822075231243871E-5
30-31	1.567481793027196E-4	3.3141043624003577E-4	0.0	0.0010121453863547038	6.046001201676328E-5
32-33	1.70183737528667E-4	3.3141043624003577E-4	0.0	0.002727418319867321	6.269927172108784E-5
34-35	1.8809781516326352E-4	3.784348900308516E-4	0.0	0.005338395135109765	7.613482994703524E-5
36-37	1.9257633457191265E-4	4.366556423432903E-4	0.0	0.009796761206419976	7.613482994703524E-5
38-39	1.9481559427623723E-4	4.5904823938653597E-4	0.0	0.02364434321796309	7.83740896513598E-5
40-41	2.104904122065092E-4	4.859193558384308E-4	0.0	0.0393415537452783	8.285260906000893E-5
42-43	2.104904122065092E-4	5.217475111076238E-4	0.0	0.04880242599604959	8.50918687643335E-5
44-45	2.104904122065092E-4	5.598149260811415E-4	0.0	0.06040179126445083	8.50918687643335E-5
46-47	2.2392597043245657E-4	5.77729003715738E-4	0.0	0.07380152133512904	8.957038817298263E-5
48-49	2.2392597043245657E-4	5.822075231243871E-4	0.0	0.08706689582354776	9.18096478773072E-5
50-51	2.4407930777137768E-4	6.269927172108784E-4	0.0	0.10021358954763729	9.404890758163176E-5
52-53	2.5079708688435136E-4	6.44906794845475E-4	0.0	0.13783539183999433	9.628816728595634E-5
54-55	2.552756062930005E-4	6.874527292276417E-4	0.0	0.1851330353147378	1.0076668669460547E-4
56-57	2.575148659973251E-4	7.098453262708873E-4	0.0	0.2475367247548548	1.052452061032546E-4
58-59	2.642326451102987E-4	7.299986636098085E-4	0.0	0.2936296465086716	1.0748446580757916E-4
60-61	2.9110376156219354E-4	7.479127412444049E-4	0.0	0.3176501853569613	1.0748446580757916E-4
62-63	3.04539319788141E-4	7.523912606530541E-4	0.0	0.33587999860986756	1.1196298521622828E-4
64-65	3.112570989011146E-4	8.285260906000894E-4	0.0	0.34129452857492437	1.1196298521622828E-4
66-67	3.246926571270621E-4	8.486794279390105E-4	0.0	0.34667099112500765	1.1196298521622828E-4
68-69	3.336496959443603E-4	8.576364667563087E-4	0.0	0.351848159561406	1.1420224492055285E-4
70-71	3.3812821535300944E-4	8.710720249822561E-4	0.0	0.35648790566876654	1.2092002403352655E-4
72-73	3.448459944659831E-4	8.800290637995543E-4	0.0	0.3603595856975437	1.3211632255514936E-4
74-75	3.470852541703077E-4	9.113786996600983E-4	0.0	0.3630287832650986	1.3435558225947394E-4
76-77	3.515637735789568E-4	9.53924634042265E-4	0.0	0.36573156972821835	1.3435558225947394E-4
78-79	3.538030332832814E-4	9.763172310855107E-4	0.0	0.3673595115332623	1.3435558225947394E-4
80-81	3.627600721005797E-4	9.807957504941599E-4	0.0	0.3678319953308748	1.4107336137244764E-4
82-83	3.6499933180490426E-4	9.830350101984846E-4	0.0	0.36824401911647053	1.4779114048542135E-4
84-85	3.7171711091787794E-4	9.987098281287565E-4	0.0	0.36846346656749435	1.4779114048542135E-4
86-87	3.7171711091787794E-4	0.0010390165028065985	0.0	0.36856423325418897	1.5003040018974592E-4
88	3.7619563032652704E-4	0.0010793231774844406	0.0	0.3685776688124149	1.567481793027196E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25395	0.0	50.65321	1
GTATCAA	46725	0.0	38.344326	1
TCAACGC	54300	0.0	31.97637	4
ATCAACG	54390	0.0	31.899433	3
CAACGCA	55715	0.0	31.140808	5
TATCAAC	57830	0.0	30.333334	2
AACGCAG	57635	0.0	30.193298	6
ACGCAGA	64920	0.0	26.751478	7
CGCAGAG	66695	0.0	26.052582	8
GCAGAGT	80220	0.0	21.703588	9
GTACATG	63830	0.0	21.633469	1
TACATGG	64420	0.0	20.65095	2
ACATGGG	63260	0.0	20.285946	3
GAGTACT	45360	0.0	19.714962	12-13
AGAGTAC	72370	0.0	19.206503	10-11
CAGAGTA	79320	0.0	18.866323	10-11
CATGGGG	43210	0.0	18.186285	4
GTGGTAT	12710	0.0	16.93063	1
GTACTTT	52615	0.0	16.84763	14-15
AGTACTT	51125	0.0	16.294767	12-13
>>END_MODULE