FastQCFastQC Report
Fri 27 May 2016
SRR2138985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7065148
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80400.053.1033941
GTATCAA152800.038.3985981
ATCAACG174250.032.6946873
TCAACGC175200.032.542324
CAACGCA177950.032.137555
AACGCAG182950.031.3301566
TATCAAC187700.030.67752
ACGCAGA205850.027.8235917
CGCAGAG217600.026.421488
GTACATG213650.023.0077931
GCAGAGT258300.022.2075779
TACATGG212350.022.1001722
ACATGGG211250.021.6159553
GAGTACT142500.020.37215612-13
CAGAGTA251150.019.80666510-11
CATGGGG150900.019.7882024
AGAGTAC232100.019.45743610-11
GTACTTT165150.017.53850714-15
AGTACTT162100.016.36038412-13
GTGGTAT41300.015.7498241
Produced by FastQC (version 0.11.5)