##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7065148
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.10305077827103	30.0	18.0	33.0	18.0	33.0
2	30.52373566696692	32.0	29.0	33.0	27.0	33.0
3	31.718709218830234	33.0	31.0	33.0	29.0	33.0
4	32.27420826853167	33.0	33.0	33.0	31.0	34.0
5	32.747837554146074	33.0	33.0	34.0	32.0	34.0
6	36.23895890079019	38.0	37.0	38.0	33.0	38.0
7	36.65902879883054	38.0	37.0	38.0	34.0	38.0
8	36.5018731383971	38.0	37.0	38.0	34.0	38.0
9	36.861907209870196	38.0	38.0	38.0	35.0	38.0
10-11	37.02438016868153	38.0	38.0	38.0	35.5	38.0
12-13	37.09443652135808	38.0	38.0	38.0	36.0	38.0
14-15	37.078660701799876	38.0	38.0	38.0	36.0	38.0
16-17	37.04896641938711	38.0	38.0	38.0	36.0	38.0
18-19	37.08773220320367	38.0	38.0	38.0	36.0	38.0
20-21	37.06761059319362	38.0	38.0	38.0	36.0	38.0
22-23	37.10736591161536	38.0	38.0	38.0	36.0	38.0
24-25	37.116420783074396	38.0	38.0	38.0	36.0	38.0
26-27	37.05333461050334	38.0	38.0	38.0	36.0	38.0
28-29	37.040969520398946	38.0	38.0	38.0	36.0	38.0
30-31	36.91190678471625	38.0	38.0	38.0	35.5	38.0
32-33	37.01316567034691	38.0	38.0	38.0	36.0	38.0
34-35	37.02574964707472	38.0	38.0	38.0	36.0	38.0
36-37	36.947646694952525	38.0	38.0	38.0	36.0	38.0
38-39	36.98742625785468	38.0	38.0	38.0	36.0	38.0
40-41	36.903308896842844	38.0	38.0	38.0	35.5	38.0
42-43	36.88636985066344	38.0	38.0	38.0	35.0	38.0
44-45	36.87840769437243	38.0	38.0	38.0	35.0	38.0
46-47	36.836435309158546	38.0	38.0	38.0	35.0	38.0
48-49	36.797854472767824	38.0	38.0	38.0	35.0	38.0
50-51	36.80849970063389	38.0	38.0	38.0	35.0	38.0
52-53	36.71367265928208	38.0	38.0	38.0	35.0	38.0
54-55	36.700652561587034	38.0	38.0	38.0	35.0	38.0
56-57	36.70197632874444	38.0	38.0	38.0	34.5	38.0
58-59	36.71767639827861	38.0	38.0	38.0	35.0	38.0
60-61	36.71414227462566	38.0	38.0	38.0	35.0	38.0
62-63	36.71975664959574	38.0	38.0	38.0	34.5	38.0
64-65	36.67258643595814	38.0	38.0	38.0	34.0	38.0
66-67	36.678856593915825	38.0	38.0	38.0	34.0	38.0
68-69	36.68915427429883	38.0	38.0	38.0	34.0	38.0
70-71	36.598427061793856	38.0	38.0	38.0	34.0	38.0
72-73	36.552780739082635	38.0	38.0	38.0	34.0	38.0
74-75	36.60244473901533	38.0	38.0	38.0	34.0	38.0
76-77	36.578306983953354	38.0	38.0	38.0	34.0	38.0
78-79	36.593294923863894	38.0	38.0	38.0	34.0	38.0
80-81	36.61361908247092	38.0	38.0	38.0	34.0	38.0
82-83	36.47846799869528	38.0	38.0	38.0	34.0	38.0
84-85	36.49176278403023	38.0	38.0	38.0	34.0	38.0
86-87	36.491107382938715	38.0	38.0	38.0	34.0	38.0
88-89	36.436566367900014	38.0	38.0	38.0	34.0	38.0
90-91	36.4305096013021	38.0	38.0	38.0	34.0	38.0
92-93	36.474316578763606	38.0	38.0	38.0	34.0	38.0
94-95	36.374272414315165	38.0	38.0	38.0	34.0	38.0
96-97	36.33190102479219	38.0	37.5	38.0	34.0	38.0
98-99	36.40472163923812	38.0	37.0	38.0	34.0	38.0
100	34.77280675345686	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	22.0
20	99.0
21	326.0
22	866.0
23	2428.0
24	5737.0
25	11270.0
26	19556.0
27	30660.0
28	45162.0
29	63565.0
30	85435.0
31	112973.0
32	148467.0
33	199147.0
34	290536.0
35	499657.0
36	1322621.0
37	4226614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25075766282603	18.434206898425906	14.368913432528235	21.946122006219827
2	14.169271471737039	21.13338602390212	42.755947929187045	21.9413945751738
3	16.85974589633508	27.69094150610858	31.069002376171028	24.38031022138531
4	11.827338931894985	19.454652613080434	39.86150042433648	28.856508030688104
5	13.223005035403524	37.63789680784433	36.18583222162853	12.95326593512362
6	28.325567748271908	38.951263151354105	19.90118535396362	12.821983746410373
7	25.17944422395681	33.3037326323525	24.77306915580537	16.74375398788532
8	23.66624166967203	36.987632813919824	22.155162213162413	17.190963303245734
9	25.411484656797	16.962036747142452	22.169188812463663	35.45728978359689
10-11	23.452771265371936	27.232670851339563	29.812708806666187	19.501849076622317
12-13	24.35125916682849	25.024670396147396	29.78617716146923	20.83789327555488
14-15	21.732531293045806	26.1602233951787	27.419602533450117	24.68764277832538
16-17	21.192889377547363	29.140005276605667	28.123204213131842	21.543901132715124
18-19	21.231437756151745	28.461498612626375	29.606216317053796	20.700847314168083
20-21	22.18201973682069	27.57720412921657	29.377469632639112	20.863306501323624
22-23	21.97938991854526	27.506285171072793	29.132570311005473	21.381754599376475
24-25	21.679084538493278	27.65601964210766	29.23779959133205	21.427096228067008
26-27	21.53923318997977	27.830901357851307	29.233325176688567	21.396540275480355
28-29	21.550547117865477	27.840011361695044	29.247522045320334	21.36191947511914
30-31	21.804824769407894	27.61516996461082	29.211469242072003	21.368536023909286
32-33	21.68569633052176	27.70847802712292	29.11570129712538	21.490124345229944
34-35	21.7419732772438	27.750714628635357	29.10787322625116	21.39943886786968
36-37	21.76751999072349	27.730648140616626	29.036660308059353	21.46517156060053
38-39	21.59172673508905	27.82280948110908	29.15964365865725	21.425820125144625
40-41	21.782028409183937	27.708124661229487	29.049339923517937	21.460507006068642
42-43	21.709710930116795	27.761501114581904	28.90338847327528	21.625399482026015
44-45	21.71660573575786	27.802005145427195	28.813325970218845	21.668063148596097
46-47	21.81035684342159	27.784988719704945	28.654716663039085	21.749937773834375
48-49	21.77192762754076	27.94279156289612	28.60062479863223	21.68465601093089
50-51	21.791073028905735	28.045439353562124	28.486441840999234	21.677045776532907
52-53	21.95196220803808	27.938442648702438	28.388197565057897	21.721397578201586
54-55	21.937954239207162	27.859279687126747	28.414128328620958	21.78863774504513
56-57	21.86839860032098	27.95629573669196	28.37412473079482	21.801180932192242
58-59	21.79071838026814	27.93196570739836	28.50487046186948	21.772445450464023
60-61	21.862750314833537	27.93856751368554	28.479442509983972	21.71923966149695
62-63	21.82669616971198	27.971300422318524	28.53514775018848	21.66685565778102
64-65	21.89914674712285	27.928578271307913	28.399964580849996	21.772310400719245
66-67	21.846725063984714	27.98715240357544	28.284942367142374	21.881180165297476
68-69	21.820671498980854	27.990789020853562	28.32473946745171	21.86380001271387
70-71	21.873514759273327	27.836375287835363	28.31191272452263	21.978197228368682
72-73	21.904827195029444	27.84155192754253	28.27695431627104	21.97666656115699
74-75	21.85186615704333	27.940990515319715	28.18960257489173	22.01754075274523
76-77	22.009322749257322	27.876753264508686	28.091591735518367	22.022332250715625
78-79	21.943848639157927	27.903049554145493	28.0806390191844	22.07246278751218
80-81	21.90136990260919	27.91004328644584	28.135729949861826	22.052856861083143
82-83	21.991387461387912	27.883081335177007	28.144337614428494	21.981193589006583
84-85	22.059731985454192	27.93643160185467	28.050396853724134	21.953439558967
86-87	21.982028219287347	27.951590642604273	28.094346502552998	21.972034635555385
88-89	22.05584408469492	27.952144319590534	28.021394144300054	21.970617451414494
90-91	22.065840880822993	28.005401751865765	28.019933191385316	21.908824175925922
92-93	22.12056420387691	27.925831408332275	28.06459418508327	21.889010202707546
94-95	21.960608751586474	28.04860543238214	28.041646712800528	21.949139103230856
96-97	22.024006740440754	28.125222165882263	28.133405414559203	21.71736567911778
98-99	23.05651695424296	30.021625241446404	28.419626665112922	18.50223113919771
100	21.911229101982023	30.906309774692176	22.441904194251087	24.740556929074714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	824.0
1	864.0
2	1500.0
3	2276.5
4	2977.0
5	4352.0
6	5501.0
7	6564.0
8	7749.5
9	8328.0
10	8076.0
11	7800.0
12	8605.0
13	10462.5
14	13720.0
15	17643.0
16	21079.0
17	23508.5
18	24080.5
19	23035.0
20	21585.5
21	21070.0
22	22755.5
23	27199.0
24	34772.0
25	45501.0
26	58821.0
27	73952.5
28	90855.5
29	109857.0
30	128112.0
31	145611.5
32	163828.5
33	180726.5
34	196697.5
35	212170.5
36	227167.5
37	241778.0
38	253982.5
39	262821.0
40	272001.0
41	280091.5
42	284244.0
43	287457.0
44	289671.5
45	292255.5
46	293305.5
47	292377.0
48	289293.5
49	281364.0
50	272068.5
51	261075.5
52	244275.0
53	224626.5
54	206464.5
55	189321.0
56	171324.5
57	152796.0
58	134475.0
59	114728.5
60	93983.5
61	75291.5
62	59113.5
63	45291.5
64	34418.5
65	25045.0
66	17910.0
67	13001.5
68	8903.5
69	5981.0
70	4244.0
71	3010.5
72	2009.5
73	1210.5
74	767.5
75	493.0
76	298.5
77	179.5
78	121.0
79	86.0
80	52.0
81	35.5
82	28.0
83	21.0
84	16.0
85	12.5
86	11.0
87	7.0
88	4.0
89	4.5
90	4.5
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0024486394340217646
6	1.4153985167755862E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.123945531841625E-5
30-31	0.0
32-33	2.1276321114152996E-5
34-35	0.0
36-37	4.843363488019442E-4
38-39	4.290131589061165E-5
40-41	2.1543388456162003E-4
42-43	5.771294561968842E-5
44-45	6.673473125669841E-4
46-47	0.0
48-49	6.61426551557869E-5
50-51	0.0
52-53	9.664465374041946E-5
54-55	0.0021525867066409994
56-57	0.001082446816907278
58-59	1.5126574638603442E-4
60-61	3.9584789057607215E-4
62-63	9.960332592361208E-5
64-65	7.481776667423843E-4
66-67	0.0012197034784311755
68-69	3.6012061848680763E-4
70-71	6.076059960769169E-4
72-73	1.2724769128946608E-4
74-75	1.282123401492616E-4
76-77	0.0
78-79	0.0014574320730806921
80-81	0.0
82-83	8.94336613395672E-4
84-85	1.5028362276706713E-4
86-87	0.0
88-89	4.246890426829476E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.106429652592884E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	50.0
20-21	168.0
22-23	403.0
24-25	558.0
26-27	1141.0
28-29	2498.0
30-31	7825.0
32-33	10799.0
34-35	16029.0
36-37	25798.0
38-39	29505.0
40-41	31045.0
42-43	35886.0
44-45	43840.0
46-47	45478.0
48-49	42263.0
50-51	37580.0
52-53	35319.0
54-55	38061.0
56-57	39526.0
58-59	42245.0
60-61	42510.0
62-63	43133.0
64-65	45350.0
66-67	48906.0
68-69	50127.0
70-71	50286.0
72-73	47456.0
74-75	47502.0
76-77	50063.0
78-79	51835.0
80-81	51390.0
82-83	49694.0
84-85	49294.0
86-87	51705.0
88-89	54032.0
90-91	54588.0
92-93	56288.0
94-95	68455.0
96-97	225243.0
98-99	508689.0
100-101	4932585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.24939367669037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.24847233843757	79.61373885452932
2	6.243194083857867	10.894297968449985
3	1.4169007169577565	3.7087118536389636
4	0.5091991169812006	1.7770925686926353
5	0.22491118026010917	0.9811682054401669
6	0.10954655199648135	0.5734722144639018
7	0.0662801137857321	0.4048029818439021
8	0.04879697590782679	0.3406005248971164
9	0.029088153111008104	0.22841313498991725
>10	0.10197508721394118	1.3485594221128443
>50	0.0012431202815603578	0.06670634467125455
>100	3.678375450698345E-4	0.0480910471388368
>500	2.4723663861355967E-5	0.014344879131107902
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4153985167755864E-5	0.0	0.0	0.0
3	0.0	1.4153985167755864E-5	0.0	0.0	0.0
4	0.0	4.246195550326759E-5	0.0	1.4153985167755864E-5	0.0
5	0.0	4.246195550326759E-5	0.0	1.4153985167755864E-5	0.0
6	5.6615940671023455E-5	4.246195550326759E-5	0.0	1.4153985167755864E-5	0.0
7	5.6615940671023455E-5	4.246195550326759E-5	0.0	1.4153985167755864E-5	0.0
8	5.6615940671023455E-5	7.076992583877931E-5	0.0	1.4153985167755864E-5	0.0
9	5.6615940671023455E-5	1.4153985167755862E-4	0.0	1.4153985167755864E-5	1.4153985167755864E-5
10-11	7.076992583877931E-5	1.4153985167755862E-4	0.0	1.4153985167755864E-5	1.4153985167755864E-5
12-13	7.076992583877931E-5	1.4153985167755862E-4	0.0	1.4153985167755864E-5	1.4153985167755864E-5
14-15	7.076992583877931E-5	1.9107879976470413E-4	0.0	1.4153985167755864E-5	2.1230977751633796E-5
16-17	9.200090359041312E-5	1.9815579234858208E-4	0.0	2.8307970335511728E-5	2.8307970335511728E-5
18-19	9.907789617429104E-5	2.1938677010021587E-4	0.0	4.246195550326759E-5	4.953894808714552E-5
20-21	9.907789617429104E-5	2.4061774785184966E-4	0.0	5.6615940671023455E-5	5.6615940671023455E-5
22-23	1.344628590936807E-4	2.972336885228731E-4	0.0	6.369293325490138E-5	5.6615940671023455E-5
24-25	1.556938368453145E-4	3.396956440261407E-4	0.0	1.0615488875816898E-4	5.6615940671023455E-5
26-27	1.840018071808262E-4	3.680036143616524E-4	0.0	1.344628590936807E-4	5.6615940671023455E-5
28-29	1.840018071808262E-4	3.680036143616524E-4	0.0	3.0431068110675104E-4	6.369293325490138E-5
30-31	1.840018071808262E-4	4.1046556986492E-4	0.0	8.492391100653518E-4	7.076992583877931E-5
32-33	1.9815579234858208E-4	4.1046556986492E-4	0.0	0.002484024396941154	7.076992583877931E-5
34-35	2.4061774785184966E-4	4.883124882875772E-4	0.0	0.0048123549570369935	7.076992583877931E-5
36-37	2.547717330196055E-4	5.803133918779904E-4	0.0	0.009129320433202533	7.784691842265725E-5
38-39	2.547717330196055E-4	6.22775347381258E-4	0.0	0.022865763038509596	9.907789617429104E-5
40-41	2.547717330196055E-4	6.935452732200373E-4	0.0	0.03890222823357699	1.1323188134204691E-4
42-43	2.760027107712393E-4	7.006222658039153E-4	0.0	0.04790416280026972	1.1323188134204691E-4
44-45	2.8307970335511725E-4	7.713921916426945E-4	0.0	0.058661191527764174	1.2738586650980275E-4
46-47	2.8307970335511725E-4	8.350851248975959E-4	0.0	0.07092561967562463	1.4861684426143655E-4
48-49	2.8307970335511725E-4	8.421621174814738E-4	0.0	0.08325374075673998	1.556938368453145E-4
50-51	3.11387673690629E-4	9.553939988235208E-4	0.0	0.09547570694909718	1.556938368453145E-4
52-53	3.2554165885838483E-4	9.624709914073987E-4	0.0	0.13279976583646938	1.6277082942919244E-4
54-55	3.2554165885838483E-4	9.695479839912766E-4	0.0	0.17909037432761493	1.6984782201307037E-4
56-57	3.3969564402614073E-4	9.766249765751544E-4	0.0	0.24223130216097383	1.9107879976470416E-4
58-59	3.3969564402614073E-4	9.978559543267883E-4	0.0	0.2893994577325203	2.1230977751633795E-4
60-61	3.538496291938966E-4	0.001019086932078422	0.0	0.30985904329251135	2.1230977751633795E-4
62-63	3.538496291938966E-4	0.0010686258801655677	0.0	0.325647813747143	2.2646376268409382E-4
64-65	3.538496291938966E-4	0.0012030887392592484	0.0	0.3311254060070645	2.2646376268409382E-4
66-67	3.7508060694553037E-4	0.0012172427244270044	0.0	0.33616422472678564	2.3354075526797174E-4
68-69	3.821575995294083E-4	0.001238473702178638	0.0	0.34109688855774856	2.4061774785184966E-4
70-71	3.821575995294083E-4	0.0012526276873463939	0.0	0.3453147761377398	2.4061774785184966E-4
72-73	3.821575995294083E-4	0.0012738586650980278	0.0	0.3486551166373302	2.476947404357276E-4
74-75	3.821575995294083E-4	0.0013517055835206848	0.0	0.35141514374504257	2.547717330196055E-4
76-77	3.821575995294083E-4	0.0014578604722788539	0.0	0.3540973239343323	2.547717330196055E-4
78-79	3.9631158469716416E-4	0.0015003224277821215	0.0	0.3559514959913083	2.547717330196055E-4
80-81	3.9631158469716416E-4	0.0015003224277821215	0.0	0.35656719434610573	2.6184872560348346E-4
82-83	3.9631158469716416E-4	0.0015073994203659994	0.0	0.3569988908937223	2.689257181873614E-4
84-85	4.246195550326759E-4	0.0015286303981176333	0.0	0.35722535465640637	2.689257181873614E-4
86-87	4.246195550326759E-4	0.0015357073907015112	0.0	0.35735981751550006	2.689257181873614E-4
88	4.3877354020043175E-4	0.001656016264627436	0.0	0.3573739715006678	2.689257181873614E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8040	0.0	53.103394	1
GTATCAA	15280	0.0	38.398598	1
ATCAACG	17425	0.0	32.694687	3
TCAACGC	17520	0.0	32.54232	4
CAACGCA	17795	0.0	32.13755	5
AACGCAG	18295	0.0	31.330156	6
TATCAAC	18770	0.0	30.6775	2
ACGCAGA	20585	0.0	27.823591	7
CGCAGAG	21760	0.0	26.42148	8
GTACATG	21365	0.0	23.007793	1
GCAGAGT	25830	0.0	22.207577	9
TACATGG	21235	0.0	22.100172	2
ACATGGG	21125	0.0	21.615955	3
GAGTACT	14250	0.0	20.372156	12-13
CAGAGTA	25115	0.0	19.806665	10-11
CATGGGG	15090	0.0	19.788202	4
AGAGTAC	23210	0.0	19.457436	10-11
GTACTTT	16515	0.0	17.538507	14-15
AGTACTT	16210	0.0	16.360384	12-13
GTGGTAT	4130	0.0	15.749824	1
>>END_MODULE