FastQCFastQC Report
Fri 27 May 2016
SRR2138984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16916004
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174550.048.483431
GTATCAA326100.037.596051
TCAACGC365650.032.3183444
ATCAACG369700.031.9524383
CAACGCA373750.031.5710035
AACGCAG389900.030.3976296
TATCAAC400800.029.8669452
ACGCAGA440900.026.8615687
CGCAGAG443150.026.7251858
GTACATG416400.021.7031671
GCAGAGT545800.021.6346449
TACATGG419400.020.8683432
ACATGGG416000.020.1745783
GAGTACT319300.020.04937412-13
AGAGTAC486400.019.44930610-11
CGAGACC144550.018.78237786-87
CAGAGTA539300.018.5944210-11
AGACCGA148100.018.56886388-89
CATGGGG305350.018.2086284
ACCGAGG156000.017.75570390-91
Produced by FastQC (version 0.11.5)