##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16916004
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.39952449762958	30.0	18.0	33.0	18.0	33.0
2	30.643929441019285	32.0	29.0	33.0	27.0	33.0
3	31.7677617006948	33.0	31.0	33.0	29.0	33.0
4	32.3069549404221	33.0	33.0	33.0	31.0	34.0
5	32.75217480440416	33.0	33.0	34.0	32.0	34.0
6	36.242163929495405	38.0	37.0	38.0	33.0	38.0
7	36.66150605071978	38.0	37.0	38.0	34.0	38.0
8	36.51793006196972	38.0	37.0	38.0	34.0	38.0
9	36.867525510161855	38.0	38.0	38.0	35.0	38.0
10-11	37.03089857983008	38.0	38.0	38.0	35.5	38.0
12-13	37.09634512382475	38.0	38.0	38.0	36.0	38.0
14-15	37.08331426854711	38.0	38.0	38.0	36.0	38.0
16-17	37.05297433129006	38.0	38.0	38.0	36.0	38.0
18-19	37.09099220477839	38.0	38.0	38.0	36.0	38.0
20-21	37.06861045127109	38.0	38.0	38.0	36.0	38.0
22-23	37.10845663250609	38.0	38.0	38.0	36.0	38.0
24-25	37.118669324750584	38.0	38.0	38.0	36.0	38.0
26-27	37.05294727187938	38.0	38.0	38.0	36.0	38.0
28-29	37.042124634862816	38.0	38.0	38.0	36.0	38.0
30-31	36.91005829120736	38.0	38.0	38.0	35.5	38.0
32-33	37.009503360107956	38.0	38.0	38.0	36.0	38.0
34-35	37.02162091952544	38.0	38.0	38.0	36.0	38.0
36-37	36.94409875401399	38.0	38.0	38.0	36.0	38.0
38-39	36.97918183213084	38.0	38.0	38.0	36.0	38.0
40-41	36.90027105292914	38.0	38.0	38.0	35.5	38.0
42-43	36.88728522878688	38.0	38.0	38.0	35.0	38.0
44-45	36.885383884599676	38.0	38.0	38.0	35.0	38.0
46-47	36.84346366273297	38.0	38.0	38.0	35.0	38.0
48-49	36.808137473558816	38.0	38.0	38.0	35.0	38.0
50-51	36.82078866176112	38.0	38.0	38.0	35.0	38.0
52-53	36.72691472727627	38.0	38.0	38.0	35.0	38.0
54-55	36.71156158877835	38.0	38.0	38.0	35.0	38.0
56-57	36.711162808955834	38.0	38.0	38.0	35.0	38.0
58-59	36.72903197878296	38.0	38.0	38.0	35.0	38.0
60-61	36.723937329934635	38.0	38.0	38.0	35.0	38.0
62-63	36.727212362255486	38.0	38.0	38.0	35.0	38.0
64-65	36.68112086928876	38.0	38.0	38.0	34.5	38.0
66-67	36.68597980094697	38.0	38.0	38.0	34.0	38.0
68-69	36.695002480531926	38.0	38.0	38.0	34.5	38.0
70-71	36.604649886473794	38.0	38.0	38.0	34.0	38.0
72-73	36.55751810542867	38.0	38.0	38.0	34.0	38.0
74-75	36.60735601034774	38.0	38.0	38.0	34.0	38.0
76-77	36.582313098411376	38.0	38.0	38.0	34.0	38.0
78-79	36.59641419602917	38.0	38.0	38.0	34.0	38.0
80-81	36.61382643388705	38.0	38.0	38.0	34.0	38.0
82-83	36.479134304956304	38.0	38.0	38.0	34.0	38.0
84-85	36.49709547917745	38.0	38.0	38.0	34.0	38.0
86-87	36.4928903389128	38.0	38.0	38.0	34.0	38.0
88-89	36.435879409592985	38.0	38.0	38.0	34.0	38.0
90-91	36.43062942145622	38.0	38.0	38.0	34.0	38.0
92-93	36.4776116081846	38.0	38.0	38.0	34.0	38.0
94-95	36.37361441771444	38.0	38.0	38.0	34.0	38.0
96-97	36.3310268967207	38.0	37.0	38.0	34.0	38.0
98-99	36.40179540291495	38.0	37.0	38.0	34.0	38.0
100	34.757538925408966	38.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	21.0
19	57.0
20	231.0
21	628.0
22	2050.0
23	5775.0
24	13465.0
25	26704.0
26	46495.0
27	73169.0
28	109155.0
29	152689.0
30	205292.0
31	270235.0
32	353099.0
33	477515.0
34	694301.0
35	1188170.0
36	3109864.0
37	1.0187087E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14776090145167	19.070449498593167	14.602248852625005	22.17954074733016
2	13.878372220767979	21.190211352515643	43.115176610268	21.816239816448377
3	16.647406798910666	27.81912915130547	31.278994731852748	24.254469317931115
4	11.712452893721235	19.68006746747045	40.26897250674568	28.33850713206263
5	13.075948418971153	37.701390296613205	36.4768460165082	12.745815267907451
6	27.888727614909975	39.36649810241817	20.135962378346704	12.608811904325155
7	24.8581461673809	33.40948607011443	25.03334711909503	16.699020643409636
8	23.228370009843932	37.3057608640906	22.50296228352748	16.96290684253799
9	25.10977769927224	17.23813732841397	22.49295992126746	35.15912505104634
10-11	23.14450564093033	27.397504753486697	30.135914486660088	19.322075118922886
12-13	24.09004809882996	25.056889913244284	30.099652967686698	20.753409020239058
14-15	21.471625331845512	26.39269002301016	27.60551487218849	24.530169772955833
16-17	21.009790491891582	29.339718765732144	28.325593325705057	21.324897416671217
18-19	21.00569673547015	28.70922707277676	29.775380166616184	20.509696025136908
20-21	21.868407522010827	27.82731670304533	29.551220462380705	20.753055312563134
22-23	21.622268486830055	27.805487802787283	29.32712584106018	21.245117869322478
24-25	21.403593180996054	27.87740081411051	29.459087209018577	21.25991879587486
26-27	21.37923392855675	28.078008803859845	29.315890104358182	21.226867163225222
28-29	21.283788239069175	28.064567654894358	29.392016460073044	21.259627645963423
30-31	21.590634072733955	27.93947298613687	29.302581258400927	21.167311682728243
32-33	21.476324135230445	27.946459991301197	29.31289208685333	21.264323786615023
34-35	21.46498963299365	27.9717167318242	29.365211671489554	21.198081963692598
36-37	21.490630416858995	28.0162533415235	29.219921175597968	21.27319506601954
38-39	21.323884875218248	28.085268799594786	29.37253993013141	21.218306395055563
40-41	21.600539923425995	27.96458032843445	29.203406297469414	21.231473450670144
42-43	21.48817410344284	27.98954570418989	29.131648558260526	21.390631634106743
44-45	21.555661505938517	28.092490452222663	28.997471438891715	21.354376602947102
46-47	21.57740156762599	28.050013763724223	28.78817882373093	21.58440584491885
48-49	21.540930819993402	28.151189995551366	28.80126018284989	21.50661900160534
50-51	21.509851318385696	28.248407987345338	28.736001151297113	21.505739542971856
52-53	21.636786308526336	28.208298854886653	28.656671257744044	21.498243578842967
54-55	21.632962546037533	28.1115029242984	28.67816365314554	21.577370876518533
56-57	21.631364862932287	28.154745577408345	28.642620521825567	21.571269037833797
58-59	21.52152617052069	28.262497415618533	28.669507664967696	21.54646874889308
60-61	21.752217656487225	28.218679596171768	28.681366573118368	21.34773617422264
62-63	21.503831067770353	28.297289944272087	28.675623092017215	21.523255895940345
64-65	21.54303699882851	28.26396948778292	28.595171733520967	21.59782177986761
66-67	21.55872873085902	28.28561282202934	28.64794120478421	21.50771724232743
68-69	21.50404484565423	28.380207834492392	28.607818815001814	21.507928504851556
70-71	21.597522705453677	28.158186709871387	28.468447019081882	21.77584356559305
72-73	21.59887134087742	28.128174650478567	28.455585617850936	21.817368390793078
74-75	21.549155776726025	28.20721638402242	28.555793669372363	21.68783416987919
76-77	21.69112884840463	28.041004229995494	28.41679190189647	21.851075019703405
78-79	21.590602274544253	28.024373393890695	28.314135920784278	22.070888410780775
80-81	21.7568639909225	28.237238083997475	28.31001997607497	21.69587794900506
82-83	21.810475741219463	28.084548306738032	28.322983786414817	21.78199216562769
84-85	21.77700232780208	28.07880228591091	28.22806637945836	21.916129006828648
86-87	21.658643149671654	28.203507050622274	28.31952302058885	21.81832677911722
88-89	21.886754194651495	28.214308208098558	28.234447894600972	21.664489702648975
90-91	21.892397923399088	28.284398063369455	28.203070454388733	21.62013355884272
92-93	21.773666252188477	28.090173471194074	28.23989138985496	21.896268886762492
94-95	21.858083086784877	28.284535351346157	28.231396430709815	21.625985131159148
96-97	22.02084336701222	28.207304818130524	28.391229840912292	21.380621973944965
98-99	22.815048054133673	30.236142880321186	28.71984487788428	18.228964187660857
100	21.688241479643438	31.110619438153993	22.705424468764136	24.495714613438434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1432.0
1	1600.0
2	3034.0
3	4805.5
4	6546.0
5	9744.5
6	12319.0
7	14125.5
8	16079.5
9	17218.5
10	17182.0
11	17080.0
12	18741.0
13	22759.5
14	30272.5
15	39361.5
16	47161.0
17	52955.5
18	55137.0
19	53395.0
20	50804.5
21	50306.5
22	54494.0
23	66242.5
24	86107.5
25	113115.0
26	148151.0
27	189627.5
28	233991.5
29	281829.0
30	329898.0
31	375520.0
32	419775.0
33	462038.5
34	504986.0
35	544163.5
36	579585.0
37	611086.5
38	631611.0
39	646490.0
40	664112.0
41	678506.5
42	687069.5
43	693913.5
44	696098.5
45	697290.5
46	698585.0
47	689614.0
48	670306.0
49	646188.5
50	616618.5
51	582831.0
52	545653.5
53	505499.5
54	464407.5
55	423926.0
56	383734.5
57	340959.5
58	297553.0
59	254286.0
60	207724.0
61	163151.0
62	125406.5
63	94404.0
64	69003.0
65	49596.0
66	35632.0
67	26092.5
68	19241.5
69	13559.5
70	9535.0
71	6819.0
72	4716.5
73	3150.5
74	2106.0
75	1402.0
76	864.5
77	483.5
78	280.5
79	161.5
80	96.5
81	61.5
82	43.5
83	36.5
84	33.5
85	29.0
86	25.5
87	17.5
88	10.0
89	8.0
90	6.5
91	4.5
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.002394182455856596
6	5.911561619399002E-6
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.912160838916704E-6
26-27	0.0
28-29	2.6611832099564446E-5
30-31	0.0
32-33	2.9613941698855294E-5
34-35	0.0
36-37	4.934526854676103E-4
38-39	4.1762441866680924E-5
40-41	1.766997217937753E-4
42-43	6.616714681458273E-5
44-45	6.499260036572999E-4
46-47	0.0
48-49	7.956002571380032E-5
50-51	6.155352037047587E-6
52-53	9.89810886731981E-5
54-55	0.001955400759385636
56-57	0.0012128425143063057
58-59	1.3816844164964577E-4
60-61	3.439805409261263E-4
62-63	1.0798617472061435E-4
64-65	6.553542925226632E-4
66-67	0.0013232418807922618
68-69	3.309372825182381E-4
70-71	6.017577409020198E-4
72-73	1.4834793313253178E-4
74-75	1.328367144206906E-4
76-77	1.0038671640136912E-5
78-79	0.0015651836236996325
80-81	0.0
82-83	0.0010323260380578484
84-85	1.8324760745181583E-4
86-87	3.4861614207781145E-6
88-89	4.92326649588793E-5
90-91	0.0
92-93	0.0
94-95	1.0852797645029734E-5
96-97	2.9377974634689475E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	106.0
20-21	441.0
22-23	909.0
24-25	1268.0
26-27	2466.0
28-29	5230.0
30-31	16584.0
32-33	22775.0
34-35	33544.0
36-37	55383.0
38-39	65573.0
40-41	68479.0
42-43	79607.0
44-45	97882.0
46-47	101656.0
48-49	95860.0
50-51	84089.0
52-53	79462.0
54-55	86783.0
56-57	93137.0
58-59	55009.0
60-61	102137.0
62-63	101005.0
64-65	108132.0
66-67	117429.0
68-69	120324.0
70-71	125692.0
72-73	111342.0
74-75	112355.0
76-77	118213.0
78-79	122354.0
80-81	122535.0
82-83	117583.0
84-85	118072.0
86-87	122533.0
88-89	128627.0
90-91	129970.0
92-93	133000.0
94-95	163719.0
96-97	583665.0
98-99	1215181.0
100-101	1.1895893E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.95034512271474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.41365413863116	72.4552947022116
2	7.619927082309235	12.48911308410325
3	2.0357961688977193	5.005025959220057
4	0.84112387689558	2.757215680101944
5	0.3934044216761189	1.6119814064579974
6	0.22105122088758722	1.0869134304921133
7	0.13503342694169523	0.7746225158682367
8	0.08242146825626867	0.5403574215297676
9	0.0595912345017523	0.4395170010336504
>10	0.19325493061158625	2.4563432676341717
>50	0.003622482398168144	0.19625347697784182
>100	0.0010502651491471793	0.13559256832323968
>500	4.158632687162563E-5	0.022875707506840457
>1k	2.7696517176044232E-5	0.02889377853926632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9115616193990026E-6	0.0	0.0	0.0	0.0
2	5.9115616193990026E-6	2.364624647759601E-5	0.0	0.0	0.0
3	5.9115616193990026E-6	2.364624647759601E-5	0.0	0.0	0.0
4	5.9115616193990026E-6	7.685030105218703E-5	0.0	1.1823123238798005E-5	0.0
5	1.1823123238798005E-5	8.276186267158604E-5	0.0	2.364624647759601E-5	0.0
6	9.458498591038404E-5	8.276186267158604E-5	0.0	2.9557808096995012E-5	0.0
7	9.458498591038404E-5	8.276186267158604E-5	0.0	2.9557808096995012E-5	0.0
8	1.0049654752978304E-4	1.0640810914918204E-4	0.0	3.546936971639401E-5	0.0
9	1.0049654752978304E-4	1.5370060210437406E-4	0.0	4.138093133579302E-5	0.0
10-11	1.1231967076858104E-4	1.5370060210437406E-4	0.0	7.389452024248754E-5	0.0
12-13	1.3005435562677805E-4	1.5961216372377307E-4	0.0	9.162920510068454E-5	0.0
14-15	1.4483325967527556E-4	2.0986043748866457E-4	0.0	1.1231967076858104E-4	2.6602027287295508E-5
16-17	1.7439106777227057E-4	2.128162182983641E-4	0.0	1.4483325967527556E-4	4.138093133579302E-5
18-19	1.7734684858197007E-4	2.275951223468616E-4	0.0	1.7143528696257106E-4	5.320405457459102E-5
20-21	1.7734684858197007E-4	2.423740263953591E-4	0.0	1.7734684858197007E-4	5.320405457459102E-5
22-23	1.8325841020136907E-4	2.807991769214526E-4	0.0	1.9803731424986658E-4	6.502717781338903E-5
24-25	2.0690465667896508E-4	3.251358890669451E-4	0.0	2.128162182983641E-4	7.980608186188653E-5
26-27	2.1577199910806358E-4	3.4878213554454115E-4	0.0	2.601087112535561E-4	9.162920510068454E-5
28-29	2.216835607274626E-4	3.5469369716394013E-4	0.0	3.9311884769003365E-4	9.754076672008355E-5
30-31	2.394182455856596E-4	3.872072860706346E-4	0.0	8.098839418576632E-4	1.0049654752978304E-4
32-33	2.482855880147581E-4	3.9607462849973316E-4	0.0	0.0019271690879240748	1.0640810914918204E-4
34-35	2.541971496341571E-4	4.699691487422207E-4	0.0	0.0035853621221654947	1.1231967076858104E-4
36-37	2.601087112535561E-4	5.438636689847082E-4	0.0	0.0066327721369656804	1.1527545157828054E-4
38-39	2.601087112535561E-4	5.911561619399003E-4	0.0	0.01860664019705836	1.2414279400737904E-4
40-41	2.660202728729551E-4	6.975642710890823E-4	0.0	0.03224461285301186	1.2414279400737904E-4
42-43	2.896665193505511E-4	7.300778599957768E-4	0.0	0.039305973207383965	1.2709857481707856E-4
44-45	2.985338617796496E-4	8.217070650964613E-4	0.0	0.048504363087168816	1.3005435562677805E-4
46-47	3.074012042087481E-4	9.044689277680474E-4	0.0	0.05825843975917717	1.4187747886557604E-4
48-49	3.074012042087481E-4	9.192478318165449E-4	0.0	0.06779970021288716	1.5370060210437406E-4
50-51	3.133127658281471E-4	0.0010049654752978304	0.0	0.07798827666392133	1.5370060210437406E-4
52-53	3.2809166987664463E-4	0.0010167885985366285	0.0	0.11266549712331589	1.6847950615287157E-4
54-55	3.4878213554454115E-4	0.001019744379346328	0.0	0.1558908356843614	1.862141910110686E-4
56-57	3.6356103959303863E-4	0.0010345232833948255	0.0	0.20696672807596878	1.9508153344016706E-4
58-59	3.7833994364153616E-4	0.0010433906258239239	0.0	0.3740451941250428	1.9508153344016706E-4
60-61	4.0198619011913214E-4	0.00106703687230152	0.0	0.5148319898718396	2.0099309505956607E-4
62-63	4.0198619011913214E-4	0.0010759042147306184	0.0	0.5210598200378765	2.128162182983641E-4
64-65	4.0198619011913214E-4	0.0011823123238798004	0.0	0.5260757800719367	2.128162182983641E-4
66-67	4.078977517385312E-4	0.001197091227928298	0.0	0.530615859395635	2.1577199910806358E-4
68-69	4.1380931335793016E-4	0.0012207374744058939	0.0	0.5355431460054041	2.1872777991776307E-4
70-71	4.1380931335793016E-4	0.0012532510633125884	0.0	0.5397728683440841	2.305509031565611E-4
72-73	4.1972087497732914E-4	0.0012768973097901843	0.0	0.5431897509600967	2.482855880147581E-4
74-75	4.3154399821612716E-4	0.0013123666795065783	0.0	0.5456371374705279	2.541971496341571E-4
76-77	4.3745555983552614E-4	0.00136261495327147	0.0	0.5482057109941567	2.541971496341571E-4
78-79	4.433671214549252E-4	0.0013833054189393665	0.0	0.5500767202466965	2.541971496341571E-4
80-81	4.433671214549252E-4	0.0013833054189393665	0.0	0.5506796995318752	2.571529304438566E-4
82-83	4.4632290226462464E-4	0.001386261199749066	0.0	0.5511880938311435	2.601087112535561E-4
84-85	4.551902446937232E-4	0.0013892169805587655	0.0	0.5514304678575389	2.601087112535561E-4
86-87	4.551902446937232E-4	0.0014128632270363616	0.0	0.5515457433091173	2.630644920632556E-4
88	4.551902446937232E-4	0.0014660672816109526	0.0	0.5515605222131658	2.660202728729551E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17455	0.0	48.48343	1
GTATCAA	32610	0.0	37.59605	1
TCAACGC	36565	0.0	32.318344	4
ATCAACG	36970	0.0	31.952438	3
CAACGCA	37375	0.0	31.571003	5
AACGCAG	38990	0.0	30.397629	6
TATCAAC	40080	0.0	29.866945	2
ACGCAGA	44090	0.0	26.861568	7
CGCAGAG	44315	0.0	26.725185	8
GTACATG	41640	0.0	21.703167	1
GCAGAGT	54580	0.0	21.634644	9
TACATGG	41940	0.0	20.868343	2
ACATGGG	41600	0.0	20.174578	3
GAGTACT	31930	0.0	20.049374	12-13
AGAGTAC	48640	0.0	19.449306	10-11
CGAGACC	14455	0.0	18.782377	86-87
CAGAGTA	53930	0.0	18.59442	10-11
AGACCGA	14810	0.0	18.568863	88-89
CATGGGG	30535	0.0	18.208628	4
ACCGAGG	15600	0.0	17.755703	90-91
>>END_MODULE