FastQCFastQC Report
Fri 27 May 2016
SRR2138983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12142203
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158550.056.3599551
GTATCAA304700.039.6537251
ATCAACG357850.032.796633
TCAACGC362150.032.4919244
CAACGCA368700.031.8552725
TATCAAC381200.031.0866182
AACGCAG382850.030.7230686
ATCTCGT134200.029.63998494
ACGCAGA423400.027.7495987
CGCAGAG436150.026.9182958
TATCTCG162500.024.55589594
GCAGAGT512050.022.9710529
GTACATG439100.022.0171831
TACATGG430850.021.7165812
ACATGGG434250.021.0116713
GAGTACT277800.020.59469612-13
CAGAGTA509100.020.16879510-11
AGAGTAC475550.019.65646410-11
CATGGGG271800.019.2358194
GTACTTT328250.017.3562414-15
Produced by FastQC (version 0.11.5)