FastQCFastQC Report
Fri 27 May 2016
SRR2138982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13356483
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136750.047.052311
CTTATAC195250.039.4965131
GTATCAA253800.037.3241581
TATACAC253250.032.6370433
TCAACGC292850.031.707084
ATCAACG294250.031.5858553
CAACGCA298950.031.0746885
TTATACA256200.030.8149362
AACGCAG312450.029.801036
TATCAAC322650.029.12992
ACGCAGA350050.026.587557
CGCAGAG361250.025.7994468
ACATCTC305800.025.6593468
ACACATC306550.025.5254696
TACACAT326450.025.38565
CACATCT334000.023.2710197
CATCTCC347300.022.467719
ATACACA399750.021.930354
GCAGAGT435200.021.375499
GTACATG332650.020.6009351
Produced by FastQC (version 0.11.5)