FastQCFastQC Report
Fri 27 May 2016
SRR2138981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22306901
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277250.052.357861
GTATCAA534100.040.2296521
ATCAACG635500.033.2604683
TCAACGC638700.033.156194
CAACGCA651950.032.5027055
AACGCAG673950.031.5257326
TATCAAC681300.031.4727752
ACGCAGA765650.027.697957
CGCAGAG766650.027.656058
CTTATAC213950.023.7883971
GCAGAGT913800.023.231639
GTACATG705350.020.956631
TACATGG704250.020.7694152
GAGTACT520550.020.59078212-13
AGAGTAC841200.020.40711610-11
ACATGGG699650.020.2670863
CAGAGTA902700.019.63451610-11
TATACAC325150.019.5320473
AGTACTT578300.017.9376512-13
TATGCCG151000.017.77896942-43
Produced by FastQC (version 0.11.5)