FastQCFastQC Report
Fri 27 May 2016
SRR2138980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7269669
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.050.470171
GTATCAA157500.037.606271
TCAACGC176400.032.6191254
ATCAACG177100.032.6157383
CAACGCA179900.032.009235
AACGCAG186500.030.995256
TATCAAC192800.030.351872
ACGCAGA208850.027.5931437
CGCAGAG214200.026.8624388
GCAGAGT259100.022.310369
GTACATG185500.020.6633131
GAGTACT154350.020.49740612-13
TACATGG183250.020.1890542
AGAGTAC233650.019.78287310-11
CAGAGTA255850.019.49146510-11
ACATGGG185400.019.4272753
CGAGACC44450.018.77042286-87
AGACCGA46700.018.14744888-89
GTACTTT180250.017.56466314-15
ACCGAGG48850.017.44165290-91
Produced by FastQC (version 0.11.5)