##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7269669
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.50304353609497	32.0	28.0	33.0	18.0	34.0
2	31.57363602111733	33.0	31.0	33.0	27.0	34.0
3	32.332709095833664	33.0	33.0	34.0	29.0	34.0
4	32.59023622671128	33.0	33.0	34.0	31.0	34.0
5	32.93937303060153	33.0	33.0	34.0	32.0	34.0
6	36.477682133808294	38.0	37.0	38.0	34.0	38.0
7	36.600997239351614	38.0	37.0	38.0	34.0	38.0
8	36.657441762479145	38.0	38.0	38.0	34.0	38.0
9	36.906706481409266	38.0	38.0	38.0	35.0	38.0
10-11	37.036349935602296	38.0	38.0	38.0	36.0	38.0
12-13	37.09855139209227	38.0	38.0	38.0	36.0	38.0
14-15	37.09936965493202	38.0	38.0	38.0	36.0	38.0
16-17	37.06123882669211	38.0	38.0	38.0	36.0	38.0
18-19	37.103206899791445	38.0	38.0	38.0	36.0	38.0
20-21	37.07567950169323	38.0	38.0	38.0	36.0	38.0
22-23	37.11640712432699	38.0	38.0	38.0	36.0	38.0
24-25	37.12446421410203	38.0	38.0	38.0	36.0	38.0
26-27	37.05951540053783	38.0	38.0	38.0	36.0	38.0
28-29	37.04681590372631	38.0	38.0	38.0	36.0	38.0
30-31	36.91719805030783	38.0	38.0	38.0	36.0	38.0
32-33	37.01591582885224	38.0	38.0	38.0	36.0	38.0
34-35	37.03091762846424	38.0	38.0	38.0	36.0	38.0
36-37	36.95511481131359	38.0	38.0	38.0	36.0	38.0
38-39	36.99125399399141	38.0	38.0	38.0	36.0	38.0
40-41	36.90656493774723	38.0	38.0	38.0	35.5	38.0
42-43	36.88797521628014	38.0	38.0	38.0	35.0	38.0
44-45	36.88596192279819	38.0	38.0	38.0	35.5	38.0
46-47	36.84317724973093	38.0	38.0	38.0	35.0	38.0
48-49	36.80749556663088	38.0	38.0	38.0	35.0	38.0
50-51	36.82360466617589	38.0	38.0	38.0	35.0	38.0
52-53	36.7345719337069	38.0	38.0	38.0	35.0	38.0
54-55	36.72034021690324	38.0	38.0	38.0	35.0	38.0
56-57	36.723653748171415	38.0	38.0	38.0	35.0	38.0
58-59	36.741967888478605	38.0	38.0	38.0	35.0	38.0
60-61	36.73728552021919	38.0	38.0	38.0	35.0	38.0
62-63	36.74057728280713	38.0	38.0	38.0	35.0	38.0
64-65	36.694310262063624	38.0	38.0	38.0	34.5	38.0
66-67	36.696997675486145	38.0	38.0	38.0	34.5	38.0
68-69	36.71029348852491	38.0	38.0	38.0	34.5	38.0
70-71	36.62603492143423	38.0	38.0	38.0	34.0	38.0
72-73	36.578453733224706	38.0	38.0	38.0	34.0	38.0
74-75	36.62572002344703	38.0	38.0	38.0	34.0	38.0
76-77	36.60149784849796	38.0	38.0	38.0	34.0	38.0
78-79	36.61805768231484	38.0	38.0	38.0	34.0	38.0
80-81	36.63771018388937	38.0	38.0	38.0	34.0	38.0
82-83	36.50673720092314	38.0	38.0	38.0	34.0	38.0
84-85	36.523806074806615	38.0	38.0	38.0	34.0	38.0
86-87	36.519275986899586	38.0	38.0	38.0	34.0	38.0
88-89	36.46773335030733	38.0	38.0	38.0	34.0	38.0
90-91	36.46396393971395	38.0	38.0	38.0	34.0	38.0
92-93	36.509737504871296	38.0	38.0	38.0	34.0	38.0
94-95	36.408830131039636	38.0	38.0	38.0	34.0	38.0
96-97	36.36790024599133	38.0	37.5	38.0	34.0	38.0
98-99	36.435836030912284	38.0	37.5	38.0	34.0	38.0
100	34.80444622829148	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	15.0
20	55.0
21	213.0
22	841.0
23	2524.0
24	6123.0
25	12234.0
26	20547.0
27	31991.0
28	47311.0
29	65774.0
30	88042.0
31	114280.0
32	148973.0
33	198504.0
34	284521.0
35	470738.0
36	1151510.0
37	4625469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.09893283999588	19.640206452315777	15.218533333498401	26.042327374189938
2	13.1514240882219	21.03069617062345	43.35996590766374	22.457913833490906
3	16.172469475570345	27.637090492015524	31.45661790103511	24.73382213137902
4	11.485364739439992	19.66650751224024	40.136435372779694	28.71169237554007
5	13.003757382636474	37.65209943789203	36.454892837422285	12.889250342049202
6	28.06658735081336	39.539915778833944	19.88805817706418	12.505438693288513
7	24.95064080634208	33.467259651024	24.84484506791162	16.737254474722302
8	23.50215670066959	37.0652639067886	22.287672244774832	17.144907147766975
9	25.0568423560529	17.06794822926552	22.291887647673544	35.58332176700804
10-11	22.979883953450976	27.412912472356034	30.154921771541453	19.452281802651537
12-13	24.012902100494536	25.11007034845741	30.13482457041717	20.742202980630893
14-15	21.45903066563278	26.448680125601314	27.627523343910156	24.464765864855746
16-17	20.922658239322864	29.424530883042955	28.35091253810868	21.3018983395255
18-19	20.95294847674633	28.687832967360688	29.939203559336747	20.42001499655624
20-21	21.802388882675793	27.876946584752844	29.670458764461436	20.650205768109924
22-23	21.582191524067326	27.797962609436404	29.485920159959335	21.133925706536935
24-25	21.348029260243027	27.902033421115895	29.56031563202586	21.18962168661522
26-27	21.230600130112677	28.112318459483493	29.47255798128191	21.18452342912192
28-29	21.216523521051872	28.037163460109486	29.527030522931003	21.219282495907635
30-31	21.49538750447986	27.895065443000068	29.48364374103124	21.12590331148883
32-33	21.361200488523817	28.005197401877652	29.436597531220333	21.1970045783782
34-35	21.411503163529247	28.009014069124227	29.463033642586627	21.116449124759896
36-37	21.38579190683902	28.006607079198904	29.386875591582616	21.22072542237946
38-39	21.306001681254894	28.093514542633326	29.444609961047608	21.155873815064176
40-41	21.488199953702853	27.893851121005262	29.370049909991263	21.247899015300618
42-43	21.383172552339193	28.01578451719649	29.253758837489606	21.34728409297471
44-45	21.469065207597517	28.066993093301747	29.130750547629606	21.33319115147113
46-47	21.528646235487358	28.040705591666548	28.911323721413428	21.519324451432663
48-49	21.472464791632234	28.182481358616833	28.922136300536494	21.42291754921444
50-51	21.452569502292462	28.280367657913736	28.812192200846038	21.454870638947764
52-53	21.563745140253083	28.27474773661902	28.70252201552589	21.458985107602008
54-55	21.551506202429614	28.176942227167995	28.739633489553242	21.531918080849152
56-57	21.567025405481058	28.25539823675673	28.69207620655872	21.48550015120349
58-59	21.45661755647356	28.275521077937327	28.761886361574824	21.505975004014292
60-61	21.642331199532837	28.23425350923897	28.784902778164657	21.33851251306353
62-63	21.49537286144175	28.30675641583796	28.74078957084416	21.457081151876135
64-65	21.508509584620743	28.23251637198933	28.675101101809947	21.583872941579983
66-67	21.537183647629664	28.237882125474627	28.69005319707116	21.534881029824547
68-69	21.47402550785093	28.304599365509603	28.671493212803227	21.54988191383624
70-71	21.50192642256898	28.11928671126355	28.60052848524165	21.778258380925816
72-73	21.555468857438402	28.148890563059425	28.55444342798376	21.741197151518413
74-75	21.499978641117835	28.265374484378537	28.579086826563472	21.655560047940163
76-77	21.620222894077436	28.039030648219505	28.509212390513284	21.83153406718978
78-79	21.526671081721783	28.107394831153865	28.407411774416953	21.958522312707395
80-81	21.697506239448824	28.212380543467486	28.37789069896643	21.712222518117258
82-83	21.711837282934944	28.09857333360656	28.44251364552527	21.747075737933226
84-85	21.717024957189178	28.120489793044612	28.347474580063736	21.815010669702474
86-87	21.58711244615273	28.206411060785097	28.41279732948439	21.79367916357779
88-89	21.781976314125455	28.199659570013218	28.33675194495557	21.681612170905755
90-91	21.80088427632702	28.27357874879476	28.337892798436883	21.587644176441334
92-93	21.75509962220018	28.08918227311689	28.345213631088917	21.810504473594012
94-95	21.730257215200062	28.29170021950943	28.364632009298397	21.613410555992107
96-97	21.91286073234545	28.273558424642886	28.431785882024858	21.381794960986802
98-99	22.749083263390123	30.26328013377841	28.750842700028688	18.236793902802784
100	21.619110641545756	31.172607879924954	22.783127230069898	24.425154248459396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	960.0
1	983.5
2	1621.5
3	2393.5
4	3013.0
5	4292.5
6	5390.0
7	6460.5
8	7688.0
9	8276.0
10	8193.0
11	8073.5
12	8780.0
13	10475.5
14	13906.5
15	18155.5
16	21426.0
17	23486.0
18	23962.5
19	22814.5
20	21343.5
21	20966.0
22	22758.0
23	27517.5
24	35280.5
25	46327.0
26	60780.0
27	77452.5
28	95611.5
29	116489.5
30	137656.5
31	157917.5
32	177611.0
33	195609.0
34	212870.0
35	227777.0
36	242085.5
37	256672.0
38	267860.0
39	275241.0
40	281943.0
41	287985.5
42	291666.0
43	293987.5
44	294272.0
45	293763.0
46	293303.5
47	290512.0
48	283477.5
49	272759.0
50	261482.0
51	247572.0
52	230135.5
53	211586.5
54	193585.5
55	175420.0
56	157120.5
57	139545.0
58	122200.0
59	103761.0
60	83550.0
61	65516.5
62	50090.5
63	36909.5
64	26744.5
65	19347.5
66	13964.0
67	10068.5
68	7360.0
69	5339.0
70	3832.5
71	2650.5
72	1795.0
73	1160.5
74	680.0
75	402.5
76	231.5
77	142.5
78	89.0
79	50.0
80	31.0
81	19.0
82	13.5
83	12.0
84	10.0
85	7.0
86	5.0
87	5.5
88	5.0
89	3.5
90	3.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0033976787663922525
6	0.0
7	0.0
8	0.0
9	2.7511568958641717E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.4401170905773886E-5
30-31	0.0
32-33	2.066396629955064E-5
34-35	0.0
36-37	6.974980606446249E-4
38-39	3.4610801878978903E-5
40-41	1.8740939276441488E-4
42-43	3.480935681080572E-5
44-45	6.359010739181192E-4
46-47	0.0
48-49	9.166266700144693E-5
50-51	0.0
52-53	9.238716276109412E-5
54-55	0.0020828886984171186
56-57	0.0013179097779021196
58-59	1.1500192987613573E-4
60-61	3.319544235236183E-4
62-63	1.45071619682563E-4
64-65	6.635081249486419E-4
66-67	0.0015179447468112162
68-69	2.7299672787597625E-4
70-71	7.277312384574771E-4
72-73	2.167448231059548E-4
74-75	1.3537301357024215E-4
76-77	7.569535646835745E-6
78-79	0.0015853094253428632
80-81	0.0
82-83	9.356623128211409E-4
84-85	2.024976843221542E-4
86-87	0.0
88-89	5.534929596486237E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.8808953098283555E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	192.0
22-23	396.0
24-25	533.0
26-27	892.0
28-29	1985.0
30-31	5085.0
32-33	6784.0
34-35	9985.0
36-37	16075.0
38-39	19087.0
40-41	20854.0
42-43	25083.0
44-45	31261.0
46-47	32627.0
48-49	29871.0
50-51	27123.0
52-53	25524.0
54-55	28112.0
56-57	30638.0
58-59	19582.0
60-61	36113.0
62-63	35135.0
64-65	38189.0
66-67	41097.0
68-69	42918.0
70-71	44259.0
72-73	41567.0
74-75	42375.0
76-77	44586.0
78-79	46514.0
80-81	47537.0
82-83	46342.0
84-85	46236.0
86-87	49084.0
88-89	51115.0
90-91	52737.0
92-93	54662.0
94-95	68402.0
96-97	250736.0
98-99	538991.0
100-101	5319340.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.3729574279774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69221526332137	76.5728967529652
2	7.2125098235552745	12.315065882305065
3	1.7685500481745398	4.52959043916157
4	0.6329732795934472	2.161552034071144
5	0.285145022250162	1.2171836922681372
6	0.1432771251269288	0.7339195142318569
7	0.0862255932133019	0.5152933729020923
8	0.046812806050714334	0.31972381584414383
9	0.03501882297170923	0.2690694434467449
>10	0.09568967782357081	1.2068844555118994
>50	0.0011677255516898877	0.06517743906800978
>100	3.151320750282549E-4	0.042689563832360264
>500	9.968029225968009E-5	0.050953594391666183
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.375578447932086E-5	0.0	0.0	0.0
3	0.0	1.375578447932086E-5	0.0	0.0	0.0
4	0.0	1.1004627583456688E-4	0.0	1.375578447932086E-5	0.0
5	0.0	1.1004627583456688E-4	0.0	1.375578447932086E-5	0.0
6	0.0	1.1004627583456688E-4	0.0	2.751156895864172E-5	0.0
7	0.0	1.1004627583456688E-4	0.0	2.751156895864172E-5	0.0
8	0.0	1.1004627583456688E-4	0.0	2.751156895864172E-5	0.0
9	0.0	1.788251982311712E-4	0.0	4.126735343796258E-5	1.375578447932086E-5
10-11	0.0	1.788251982311712E-4	0.0	9.629049135524603E-5	1.375578447932086E-5
12-13	0.0	1.788251982311712E-4	0.0	1.375578447932086E-4	2.063367671898129E-5
14-15	0.0	2.063367671898129E-4	0.0	1.6506941375185033E-4	2.751156895864172E-5
16-17	0.0	2.063367671898129E-4	0.0	1.788251982311712E-4	2.751156895864172E-5
18-19	0.0	2.1321465942947333E-4	0.0	1.9258098271049206E-4	3.438946119830215E-5
20-21	0.0	2.2009255166913376E-4	0.0	1.994588749501525E-4	4.126735343796258E-5
22-23	0.0	2.2009255166913376E-4	0.0	2.2697044390879417E-4	4.126735343796258E-5
24-25	0.0	2.4760412062777546E-4	0.0	2.4760412062777546E-4	4.126735343796258E-5
26-27	0.0	2.6135990510709633E-4	0.0	3.163830430243798E-4	4.126735343796258E-5
28-29	0.0	2.6135990510709633E-4	0.0	3.98917749900305E-4	4.126735343796258E-5
30-31	0.0	3.3013882750370066E-4	0.0	7.015450084453638E-4	4.126735343796258E-5
32-33	0.0	3.3013882750370066E-4	0.0	0.001808885659030693	5.502313791728344E-5
34-35	0.0	3.851619654209841E-4	0.0	0.002730523219145191	7.565681463626473E-5
36-37	0.0	4.4706299557792796E-4	0.0	0.004663210938489772	8.253470687592516E-5
38-39	0.0	4.539408878175884E-4	0.0	0.012572787014099265	8.253470687592516E-5
40-41	0.0	4.6769667229690925E-4	0.0	0.022153690903946244	8.253470687592516E-5
42-43	0.0	4.8145245677623006E-4	0.0	0.026975093363948207	8.253470687592516E-5
44-45	0.0	5.43353486933174E-4	0.0	0.033399044715791054	8.253470687592516E-5
46-47	0.0	5.91498732610797E-4	0.0	0.04051766318383959	8.253470687592516E-5
48-49	0.0	5.91498732610797E-4	0.0	0.047849496311317616	8.253470687592516E-5
50-51	0.0	6.87789223966043E-4	0.0	0.05566965978781152	8.253470687592516E-5
52-53	0.0	6.87789223966043E-4	0.0	0.08188818500539709	8.94125991155856E-5
54-55	0.0	6.87789223966043E-4	0.0	0.11338893146304185	9.629049135524603E-5
56-57	0.0	6.87789223966043E-4	0.0	0.152537894091189	9.629049135524603E-5
58-59	0.0	7.015450084453639E-4	0.0	0.27799064854259525	9.629049135524603E-5
60-61	0.0	7.153007929246848E-4	0.0	0.3831467429947636	9.629049135524603E-5
62-63	0.0	7.428123618833265E-4	0.0	0.3885183768339384	1.1004627583456688E-4
64-65	0.0	7.978354998006099E-4	0.0	0.3929133499750814	1.1004627583456688E-4
66-67	0.0	7.978354998006099E-4	0.0	0.3970194516421587	1.169241680742273E-4
68-69	0.0	8.184691765195911E-4	0.0	0.40080917026621155	1.2380206031388773E-4
70-71	0.0	8.253470687592516E-4	0.0	0.4042206048170831	1.2380206031388773E-4
72-73	0.0	8.253470687592516E-4	0.0	0.4069648838207076	1.5131362927252946E-4
74-75	0.0	8.872480989161955E-4	0.0	0.4089250831090109	1.5131362927252946E-4
76-77	0.0	9.42271236833479E-4	0.0	0.41140112431528864	1.581915215121899E-4
78-79	0.0	9.76660698031781E-4	0.0	0.4129555279614519	1.6506941375185033E-4
80-81	0.0	9.76660698031781E-4	0.0	0.41360204983198	1.7194730599151076E-4
82-83	0.0	9.76660698031781E-4	0.0	0.4140628686120372	1.788251982311712E-4
84-85	0.0	9.76660698031781E-4	0.0	0.4143448621938633	1.788251982311712E-4
86-87	0.0	9.835385902714415E-4	0.0	0.41448242003865654	1.788251982311712E-4
88	0.0	0.0010454396204283853	0.0	0.4145030537153755	1.788251982311712E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8370	0.0	50.47017	1
GTATCAA	15750	0.0	37.60627	1
TCAACGC	17640	0.0	32.619125	4
ATCAACG	17710	0.0	32.615738	3
CAACGCA	17990	0.0	32.00923	5
AACGCAG	18650	0.0	30.99525	6
TATCAAC	19280	0.0	30.35187	2
ACGCAGA	20885	0.0	27.593143	7
CGCAGAG	21420	0.0	26.862438	8
GCAGAGT	25910	0.0	22.31036	9
GTACATG	18550	0.0	20.663313	1
GAGTACT	15435	0.0	20.497406	12-13
TACATGG	18325	0.0	20.189054	2
AGAGTAC	23365	0.0	19.782873	10-11
CAGAGTA	25585	0.0	19.491465	10-11
ACATGGG	18540	0.0	19.427275	3
CGAGACC	4445	0.0	18.770422	86-87
AGACCGA	4670	0.0	18.147448	88-89
GTACTTT	18025	0.0	17.564663	14-15
ACCGAGG	4885	0.0	17.441652	90-91
>>END_MODULE