FastQCFastQC Report
Fri 27 May 2016
SRR2138979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6140273
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.051.305621
GTATCAA120700.037.2598461
TCAACGC142850.030.4918214
ATCAACG143650.030.3835743
CAACGCA147650.029.5005515
AACGCAG152500.028.705926
TATCAAC156250.028.3579622
ACGCAGA171250.025.408017
CGCAGAG176600.024.663338
ATCTCGT77300.023.89708594
GTACATG162100.022.424161
ACATGGG162300.021.5518883
TACATGG165850.021.2772142
GCAGAGT213550.020.3544839
TATCTCG80600.020.1499794
GAGTACT119450.019.41625612-13
CATGGGG114450.019.3188634
AGAGTAC191350.019.2149810-11
CAGAGTA209800.018.42095210-11
AGTACTT135750.016.77542512-13
Produced by FastQC (version 0.11.5)