FastQCFastQC Report
Fri 27 May 2016
SRR2138978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7434620
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89200.054.637411
GTATCAA163900.037.5266461
TCAACGC210550.029.0652454
ATCAACG212050.028.7346173
CAACGCA215050.028.415955
AACGCAG220250.027.7842526
TATCAAC223100.027.3708272
ACGCAGA257450.023.7867557
CGCAGAG263550.023.2697268
AGAGTAC277200.019.92423210-11
GCAGAGT308600.019.915729
GTACATG216800.019.0560021
GAGTACT178950.018.6291212-13
TACATGG221600.018.6232972
ACATGGG224050.018.104113
CAGAGTA306000.017.87574210-11
AGTACTT198900.017.2604112-13
GTGGTAT43700.016.9866311
TGGTATC40850.016.5492692
GTACTTT202300.016.28238714-15
Produced by FastQC (version 0.11.5)