##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7434620
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.920546981553866	30.0	18.0	32.0	18.0	33.0
2	27.082389954025896	29.0	18.0	32.0	18.0	33.0
3	29.511075616507636	31.0	28.0	33.0	25.0	33.0
4	29.496654570105804	31.0	29.0	33.0	25.0	33.0
5	32.060274903088526	33.0	32.0	33.0	32.0	33.0
6	36.031409809781806	38.0	36.0	38.0	33.0	38.0
7	36.5474675235587	38.0	37.0	38.0	34.0	38.0
8	36.884124272659534	38.0	38.0	38.0	35.0	38.0
9	36.983185556222104	38.0	38.0	38.0	35.0	38.0
10-11	37.07630363085134	38.0	38.0	38.0	36.0	38.0
12-13	37.13204877989729	38.0	38.0	38.0	36.0	38.0
14-15	37.14082004191202	38.0	38.0	38.0	36.0	38.0
16-17	37.119855957668314	38.0	38.0	38.0	36.0	38.0
18-19	37.15500550128991	38.0	38.0	38.0	36.0	38.0
20-21	37.122802219299416	38.0	38.0	38.0	36.0	38.0
22-23	37.15924729296515	38.0	38.0	38.0	36.0	38.0
24-25	37.16723548222929	38.0	38.0	38.0	36.0	38.0
26-27	37.10250294720844	38.0	38.0	38.0	36.0	38.0
28-29	37.09074067662365	38.0	38.0	38.0	36.0	38.0
30-31	36.9580350153058	38.0	38.0	38.0	36.0	38.0
32-33	37.05139369101191	38.0	38.0	38.0	36.0	38.0
34-35	37.06572439982217	38.0	38.0	38.0	36.0	38.0
36-37	36.98507524889824	38.0	38.0	38.0	36.0	38.0
38-39	37.025213991660905	38.0	38.0	38.0	36.0	38.0
40-41	36.94129804849607	38.0	38.0	38.0	35.5	38.0
42-43	36.927653735826695	38.0	38.0	38.0	35.5	38.0
44-45	36.924558319141966	38.0	38.0	38.0	36.0	38.0
46-47	36.8799040722111	38.0	38.0	38.0	35.0	38.0
48-49	36.8418450912486	38.0	38.0	38.0	35.0	38.0
50-51	36.85878315757111	38.0	38.0	38.0	35.0	38.0
52-53	36.77326064960051	38.0	38.0	38.0	35.0	38.0
54-55	36.7611417900422	38.0	38.0	38.0	35.0	38.0
56-57	36.77005235818157	38.0	38.0	38.0	35.0	38.0
58-59	36.79377370215019	38.0	38.0	38.0	35.0	38.0
60-61	36.794363901539135	38.0	38.0	38.0	35.0	38.0
62-63	36.80470120146264	38.0	38.0	38.0	35.0	38.0
64-65	36.762316742620456	38.0	38.0	38.0	35.0	38.0
66-67	36.76995034670221	38.0	38.0	38.0	35.0	38.0
68-69	36.78658259021361	38.0	38.0	38.0	35.0	38.0
70-71	36.70482361413723	38.0	38.0	38.0	34.5	38.0
72-73	36.66258976606038	38.0	38.0	38.0	34.0	38.0
74-75	36.71795532058013	38.0	38.0	38.0	34.5	38.0
76-77	36.6971317351985	38.0	38.0	38.0	34.5	38.0
78-79	36.71539865399032	38.0	38.0	38.0	34.5	38.0
80-81	36.74176064652352	38.0	38.0	38.0	35.0	38.0
82-83	36.614301566516595	38.0	38.0	38.0	34.0	38.0
84-85	36.633232294359004	38.0	38.0	38.0	34.0	38.0
86-87	36.63474857368391	38.0	38.0	38.0	34.0	38.0
88-89	36.584638066327116	38.0	38.0	38.0	34.0	38.0
90-91	36.58515696581807	38.0	38.0	38.0	34.0	38.0
92-93	36.63451410910726	38.0	38.0	38.0	34.0	38.0
94-95	36.5335763415428	38.0	38.0	38.0	34.0	38.0
96-97	36.493786708186306	38.0	38.0	38.0	34.0	38.0
98-99	36.56571599402899	38.0	38.0	38.0	34.0	38.0
100	35.021437736314034	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	15.0
19	47.0
20	90.0
21	226.0
22	750.0
23	2158.0
24	5217.0
25	10506.0
26	18258.0
27	29706.0
28	45496.0
29	65114.0
30	88501.0
31	116122.0
32	152407.0
33	206651.0
34	307301.0
35	552683.0
36	1547890.0
37	4285477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.01342637552423	22.17872601424148	15.008917738902595	23.79892987133169
2	13.701440019799263	22.99599710543377	40.320379521750944	22.982183353016026
3	17.1250985255467	27.31880042288644	31.444095326997214	24.11200572456965
4	12.435981933172105	18.83094226739228	39.80091786802822	28.93215793140739
5	12.936226146478258	38.51634551698794	35.59176533950865	12.955662997025152
6	28.692001567262558	39.12569023375643	19.282010281898778	12.900297917082234
7	25.163317022255338	33.43914013090111	24.513572986917957	16.88396985992559
8	23.96246210297231	36.91517522079138	21.888341300564118	17.234021375672192
9	25.392612587141315	16.61892828670844	21.984206588125094	36.00425253802515
10-11	23.498322711853465	27.205411978016365	29.776303294586675	19.5199620155435
12-13	24.394515119804375	24.990214698262992	29.772342096838845	20.84292808509379
14-15	21.751394153299024	26.23744051478085	27.292854241373465	24.718311090546656
16-17	21.169703360763563	29.122322324476567	28.083762182868792	21.62421213189107
18-19	21.255269267292746	28.365168630003957	29.722823762344277	20.656738340359023
20-21	22.16059842323749	27.48616844020223	29.500752400536012	20.852480736024265
22-23	21.9297975408653	27.397517782918257	29.32857570799763	21.344108968218816
24-25	21.714262192338843	27.55532947069912	29.35666572098447	21.373742615977562
26-27	21.510666430876306	27.722637078877593	29.378790827288515	21.38790566295759
28-29	21.54087899044862	27.589435774148193	29.483538801312807	21.386146434090385
30-31	21.78551015479681	27.56397951406342	29.340749250273195	21.309761080866572
32-33	21.657987473561953	27.644414493634994	29.233129193897344	21.464468838905706
34-35	21.76034860562404	27.624892859761058	29.272598286605948	21.342160248008955
36-37	21.68199383832227	27.589733257688977	29.25130798328392	21.476964920704834
38-39	21.58364006439964	27.726169309587174	29.301455251316693	21.388735374696495
40-41	21.79791628995973	27.561649640362518	29.210673001934516	21.42976106774324
42-43	21.70859807061591	27.661888862360428	28.994900763487845	21.634612303535814
44-45	21.751097702506055	27.687995075194742	28.931341357684246	21.629565864614957
46-47	21.870426500218024	27.685841881083796	28.6936813357893	21.75005028290888
48-49	21.75817145797442	27.836003034752338	28.644472677200696	21.761352830072543
50-51	21.794187253171447	27.916271919334374	28.530007027973337	21.75953379952084
52-53	21.931201519759533	27.87401753911126	28.438847729665223	21.75593321146398
54-55	21.925316685317025	27.738595016034918	28.45689595314445	21.879192345503608
56-57	21.832855717041326	27.871463704551818	28.471918069351474	21.823762509055378
58-59	21.800480264620827	27.905783431662723	28.52174487469722	21.771991429019234
60-61	21.893684256849884	27.94791401766225	28.490385095954952	21.668016629532914
62-63	21.838061799419116	28.011006746674582	28.480711034520123	21.67022041938618
64-65	21.87766621244769	27.907081719020688	28.385118561503496	21.830133507028126
66-67	21.872452129694082	27.98801354504996	28.279646143670806	21.859888181585145
68-69	21.817598268912413	27.938293882151953	28.34944320600777	21.894664642927864
70-71	21.841217790933843	27.750459093498552	28.429830189131778	21.978492926435827
72-73	21.94191720300524	27.79044907357955	28.239021809452176	22.028611913963033
74-75	21.841954884623576	27.913852607555956	28.21821570804226	22.025976799778206
76-77	21.97799391489951	27.779186955265217	28.208927425212554	22.033891704622718
78-79	21.958322710815697	27.79871431258472	28.10867176944875	22.134291207150827
80-81	21.942019960790084	27.905675705780403	28.078930771345057	22.07337356208446
82-83	22.021293077964334	27.885402397122512	28.088692398803627	22.004612126109528
84-85	22.051244756609563	27.918268036359834	28.031678284847946	21.998808922182658
86-87	22.005205415673885	27.85606960306138	28.119265826629718	22.01945915463502
88-89	22.03720223863964	27.88317977080908	28.07209450820622	22.00752348234506
90-91	22.088152477411292	27.906841835891193	28.077581258519064	21.927424428178448
92-93	22.125859315106307	27.882944140735198	28.045414812850506	21.945781731307985
94-95	22.010324332424513	27.90249221894472	28.07139377681634	22.01578967181443
96-97	22.163961307438615	27.981880917300234	28.108273149866527	21.745884625394623
98-99	23.132525039647724	29.881213859165346	28.353091322811895	18.633169778375034
100	22.01919723272921	30.795040432151175	22.38598439085388	24.799777944265724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1497.0
1	1483.0
2	2071.5
3	2996.0
4	3752.5
5	5000.0
6	6253.5
7	8196.0
8	10193.5
9	10863.5
10	10689.0
11	10507.0
12	11417.5
13	13648.5
14	18264.0
15	23687.5
16	26993.5
17	27884.5
18	27121.0
19	25251.5
20	23234.5
21	22411.0
22	23994.0
23	28725.0
24	35959.0
25	46598.0
26	59962.5
27	74950.5
28	91599.5
29	110605.0
30	130219.0
31	148340.0
32	167552.0
33	186766.0
34	204161.5
35	219497.0
36	236037.0
37	251447.5
38	263003.0
39	273423.0
40	282823.0
41	289195.5
42	290378.5
43	292108.5
44	295188.5
45	296935.5
46	293717.5
47	285439.0
48	278847.5
49	273755.5
50	268150.0
51	259885.0
52	248012.5
53	232476.0
54	215390.0
55	199026.5
56	182495.5
57	163897.0
58	144067.5
59	124034.5
60	102950.0
61	82656.5
62	64222.0
63	48351.0
64	35053.5
65	24997.5
66	18168.0
67	13564.0
68	10134.0
69	7281.0
70	5241.0
71	3663.5
72	2473.5
73	1621.0
74	966.0
75	545.5
76	314.0
77	173.5
78	102.0
79	55.5
80	36.5
81	29.0
82	24.0
83	12.5
84	4.5
85	3.5
86	3.0
87	4.0
88	4.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.003873768935063258
6	1.3450586580080757E-5
7	0.0
8	0.0
9	1.3450586580080757E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.017813662575953E-5
26-27	0.0
28-29	1.3455588115836198E-5
30-31	0.0
32-33	2.0208708808336227E-5
34-35	0.0
36-37	4.864565431141367E-4
38-39	4.74203948750476E-5
40-41	2.0385452207428498E-4
42-43	6.817703339365638E-5
44-45	7.119885731310848E-4
46-47	0.0
48-49	5.532817979002679E-5
50-51	2.0846268153190885E-5
52-53	1.5350518983603065E-4
54-55	0.0020879183167118454
56-57	0.0011049673985351369
58-59	1.697445118770235E-4
60-61	5.118765672220836E-4
62-63	1.2149974424303837E-4
64-65	8.122111124999362E-4
66-67	0.0013236454062452194
68-69	3.641376553948341E-4
70-71	6.96786012174832E-4
72-73	2.5115991558662977E-4
74-75	2.1566474157377403E-4
76-77	1.4977969279436111E-5
78-79	0.0017126512263488146
80-81	0.0
82-83	9.501232784953847E-4
84-85	1.466842451007269E-4
86-87	7.780424591774737E-6
88-89	1.0983402901422782E-4
90-91	0.0
92-93	0.0
94-95	1.6121942503832792E-5
96-97	2.2083626111777662E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	40.0
20-21	234.0
22-23	447.0
24-25	548.0
26-27	1029.0
28-29	2306.0
30-31	5710.0
32-33	8013.0
34-35	11704.0
36-37	18481.0
38-39	22172.0
40-41	23539.0
42-43	28369.0
44-45	35914.0
46-47	37773.0
48-49	34799.0
50-51	30620.0
52-53	28915.0
54-55	31603.0
56-57	34012.0
58-59	36154.0
60-61	36870.0
62-63	38821.0
64-65	42515.0
66-67	46355.0
68-69	48089.0
70-71	49658.0
72-73	45201.0
74-75	46136.0
76-77	48792.0
78-79	50686.0
80-81	51264.0
82-83	48921.0
84-85	49681.0
86-87	52531.0
88-89	54709.0
90-91	56266.0
92-93	57175.0
94-95	70468.0
96-97	240361.0
98-99	543320.0
100-101	5364419.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.81014645114804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.78344062825889	76.1454674857059
2	7.072726771067289	11.996779865263443
3	1.7465640394820972	4.443790559243561
4	0.6328636929044383	2.1469304991535916
5	0.29013261765575493	1.2303094896819755
6	0.14935267037918476	0.759997310863722
7	0.08893007036841605	0.5279520604299386
8	0.056253448541121465	0.3816690567323705
9	0.040622363587665286	0.31006697445555087
>10	0.1356400777860923	1.7789397851024191
>50	0.002803954753280263	0.16001410325661142
>100	6.163363607283104E-4	0.08922716859298474
>500	5.332885504553888E-5	0.028855641517980805
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6901173160161515E-5	0.0	0.0	0.0
3	0.0	2.6901173160161515E-5	0.0	0.0	0.0
4	0.0	1.3450586580080758E-4	0.0	2.6901173160161515E-5	0.0
5	0.0	1.3450586580080758E-4	0.0	2.6901173160161515E-5	0.0
6	0.0	1.4795645238088834E-4	0.0	2.6901173160161515E-5	1.3450586580080757E-5
7	0.0	1.4795645238088834E-4	0.0	2.6901173160161515E-5	1.3450586580080757E-5
8	0.0	2.0175879870121136E-4	0.0	2.6901173160161515E-5	1.3450586580080757E-5
9	0.0	3.766164242422612E-4	0.0	2.6901173160161515E-5	1.3450586580080757E-5
10-11	0.0	3.766164242422612E-4	0.0	3.362646645020189E-5	1.3450586580080757E-5
12-13	0.0	3.766164242422612E-4	0.0	4.035175974024227E-5	1.3450586580080757E-5
14-15	0.0	4.3041877056258424E-4	0.0	4.035175974024227E-5	2.6901173160161515E-5
16-17	0.0	4.43869357142665E-4	0.0	4.035175974024227E-5	4.035175974024227E-5
18-19	0.0	4.774958235928669E-4	0.0	5.380234632032303E-5	4.707705303028265E-5
20-21	0.0	5.245728766231496E-4	0.0	5.380234632032303E-5	5.380234632032303E-5
22-23	0.0	5.85100516233513E-4	0.0	6.052763961036341E-5	6.052763961036341E-5
24-25	0.0	6.254522759737552E-4	0.0	7.397822619044416E-5	8.070351948048454E-5
26-27	0.0	6.456281558438763E-4	0.0	1.0087939935060568E-4	9.41541060605653E-5
28-29	0.0	6.456281558438763E-4	0.0	2.48835851731494E-4	9.41541060605653E-5
30-31	1.3450586580080757E-5	7.599581417745628E-4	0.0	6.725293290040378E-4	9.41541060605653E-5
32-33	1.3450586580080757E-5	7.801340216446839E-4	0.0	0.0014728392305188428	9.41541060605653E-5
34-35	1.3450586580080757E-5	9.079145941554511E-4	0.0	0.0027304690757563935	1.0760469264064606E-4
36-37	1.3450586580080757E-5	0.0010356951666662183	0.0	0.005265904646101617	1.0760469264064606E-4
38-39	1.3450586580080757E-5	0.0010356951666662183	0.0	0.013733048898262453	1.0760469264064606E-4
40-41	1.3450586580080757E-5	0.0012105527922072683	0.0	0.02324933890366959	1.0760469264064606E-4
42-43	1.3450586580080757E-5	0.0012307286720773893	0.0	0.02930210286470593	1.0760469264064606E-4
44-45	1.3450586580080757E-5	0.0013316080714279948	0.0	0.0368411566428412	1.0760469264064606E-4
46-47	1.3450586580080757E-5	0.0014055862976184391	0.0	0.04504601445669046	1.1432998593068644E-4
48-49	1.3450586580080757E-5	0.0014123115909084796	0.0	0.053129816991318995	1.2105527922072682E-4
50-51	1.3450586580080757E-5	0.0015468174567092872	0.0	0.061596961243479825	1.277805725107672E-4
52-53	1.3450586580080757E-5	0.0015468174567092872	0.0	0.08793320976727795	1.4123115909084796E-4
54-55	1.3450586580080757E-5	0.0015468174567092872	0.0	0.12088714688847581	1.4795645238088834E-4
56-57	1.3450586580080757E-5	0.0015468174567092872	0.0	0.16367346279971268	1.4795645238088834E-4
58-59	1.3450586580080757E-5	0.0015468174567092872	0.0	0.1968896863592221	1.4795645238088834E-4
60-61	1.3450586580080757E-5	0.001634246269479812	0.0	0.21539769349341326	1.4795645238088834E-4
62-63	1.3450586580080757E-5	0.0016745980292200541	0.0	0.22981672230725983	1.4795645238088834E-4
64-65	1.3450586580080757E-5	0.0018763568279212656	0.0	0.23439664703777732	1.4795645238088834E-4
66-67	1.3450586580080757E-5	0.0019032580010814272	0.0	0.23841837242522146	1.4795645238088834E-4
68-69	2.6901173160161515E-5	0.0019637856406917904	0.0	0.24244682310595567	1.4795645238088834E-4
70-71	2.6901173160161515E-5	0.0020108626937220733	0.0	0.24665685670552093	1.4795645238088834E-4
72-73	2.6901173160161515E-5	0.0020175879870121135	0.0	0.2499522504176407	2.0175879870121136E-4
74-75	2.6901173160161515E-5	0.0020848409199125174	0.0	0.2522859271892847	2.0175879870121136E-4
76-77	2.6901173160161515E-5	0.0022126214924232846	0.0	0.2545523510280283	2.0175879870121136E-4
78-79	2.6901173160161515E-5	0.0022664238387436076	0.0	0.2560520914317074	2.0175879870121136E-4
80-81	2.6901173160161515E-5	0.002279874425323688	0.0	0.25663046665465084	2.1520938528129212E-4
82-83	2.6901173160161515E-5	0.0022933250119037693	0.0	0.2571348636514038	2.2865997186137288E-4
84-85	2.6901173160161515E-5	0.0023067755984838498	0.0	0.25735679832997516	2.2865997186137288E-4
86-87	2.6901173160161515E-5	0.002380753824674294	0.0	0.25744422714274573	2.2865997186137288E-4
88	2.6901173160161515E-5	0.0025152596904751017	0.0	0.25745767772932576	2.2865997186137288E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8920	0.0	54.63741	1
GTATCAA	16390	0.0	37.526646	1
TCAACGC	21055	0.0	29.065245	4
ATCAACG	21205	0.0	28.734617	3
CAACGCA	21505	0.0	28.41595	5
AACGCAG	22025	0.0	27.784252	6
TATCAAC	22310	0.0	27.370827	2
ACGCAGA	25745	0.0	23.786755	7
CGCAGAG	26355	0.0	23.269726	8
AGAGTAC	27720	0.0	19.924232	10-11
GCAGAGT	30860	0.0	19.91572	9
GTACATG	21680	0.0	19.056002	1
GAGTACT	17895	0.0	18.62912	12-13
TACATGG	22160	0.0	18.623297	2
ACATGGG	22405	0.0	18.10411	3
CAGAGTA	30600	0.0	17.875742	10-11
AGTACTT	19890	0.0	17.26041	12-13
GTGGTAT	4370	0.0	16.986631	1
TGGTATC	4085	0.0	16.549269	2
GTACTTT	20230	0.0	16.282387	14-15
>>END_MODULE