FastQCFastQC Report
Fri 27 May 2016
SRR2138977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12786969
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124900.046.3982541
GTATCAA222100.035.8522761
TCAACGC266100.029.7245584
ATCAACG267550.029.5303963
CAACGCA274100.028.8731445
AACGCAG286550.027.7253236
TATCAAC297100.026.7868292
ACGCAGA324250.024.3926057
CGCAGAG333050.023.7480958
AGAGTAC365600.019.21387710-11
GCAGAGT411650.019.213669
GTACATG300850.017.4833491
TACATGG299350.017.304952
GAGTACT244550.017.22931512-13
CAGAGTA404250.016.88990810-11
ACATGGG303550.016.74493
AGTACTT281350.016.0291712-13
TATGCCG48250.015.13179842-43
GTGGTAT75950.015.0856741
CATGGGG223300.014.6006284
Produced by FastQC (version 0.11.5)