##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12786969
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.489951371587747	30.0	18.0	32.0	18.0	33.0
2	26.831289338388167	29.0	18.0	32.0	18.0	33.0
3	29.3912069388766	31.0	27.0	33.0	25.0	33.0
4	29.3937404556154	31.0	29.0	33.0	25.0	33.0
5	32.065390946048275	33.0	32.0	33.0	32.0	33.0
6	36.0480938054984	38.0	36.0	38.0	33.0	38.0
7	36.57921849970857	38.0	37.0	38.0	34.0	38.0
8	36.911772836862276	38.0	38.0	38.0	35.0	38.0
9	37.00283499553334	38.0	38.0	38.0	35.0	38.0
10-11	37.10611686006277	38.0	38.0	38.0	36.0	38.0
12-13	37.15999956674642	38.0	38.0	38.0	36.0	38.0
14-15	37.167979252941024	38.0	38.0	38.0	36.0	38.0
16-17	37.14476198386029	38.0	38.0	38.0	36.0	38.0
18-19	37.17839157191982	38.0	38.0	38.0	36.0	38.0
20-21	37.14528423829822	38.0	38.0	38.0	36.0	38.0
22-23	37.181496751711364	38.0	38.0	38.0	36.0	38.0
24-25	37.189499215035795	38.0	38.0	38.0	36.0	38.0
26-27	37.1260237925908	38.0	38.0	38.0	36.0	38.0
28-29	37.114352138881685	38.0	38.0	38.0	36.0	38.0
30-31	36.984391426436986	38.0	38.0	38.0	36.0	38.0
32-33	37.078673967005585	38.0	38.0	38.0	36.0	38.0
34-35	37.09052098030712	38.0	38.0	38.0	36.0	38.0
36-37	37.01192132090521	38.0	38.0	38.0	36.0	38.0
38-39	37.048226234218326	38.0	38.0	38.0	36.0	38.0
40-41	36.9674732495241	38.0	38.0	38.0	36.0	38.0
42-43	36.95948665322403	38.0	38.0	38.0	36.0	38.0
44-45	36.96082873282052	38.0	38.0	38.0	36.0	38.0
46-47	36.92285438206957	38.0	38.0	38.0	36.0	38.0
48-49	36.890411074135585	38.0	38.0	38.0	35.5	38.0
50-51	36.90955393081434	38.0	38.0	38.0	36.0	38.0
52-53	36.82046207750277	38.0	38.0	38.0	35.0	38.0
54-55	36.80771955971778	38.0	38.0	38.0	35.0	38.0
56-57	36.81376654653828	38.0	38.0	38.0	35.0	38.0
58-59	36.83462257341766	38.0	38.0	38.0	35.0	38.0
60-61	36.830371976218736	38.0	38.0	38.0	35.0	38.0
62-63	36.83554743466337	38.0	38.0	38.0	35.0	38.0
64-65	36.790835195790095	38.0	38.0	38.0	35.0	38.0
66-67	36.79535960300343	38.0	38.0	38.0	35.0	38.0
68-69	36.80872113768222	38.0	38.0	38.0	35.0	38.0
70-71	36.72495080764162	38.0	38.0	38.0	35.0	38.0
72-73	36.680090361694326	38.0	38.0	38.0	34.5	38.0
74-75	36.73411838531388	38.0	38.0	38.0	35.0	38.0
76-77	36.7145509162893	38.0	38.0	38.0	34.5	38.0
78-79	36.72970180947665	38.0	38.0	38.0	35.0	38.0
80-81	36.75483276889522	38.0	38.0	38.0	35.0	38.0
82-83	36.630059017781846	38.0	38.0	38.0	34.0	38.0
84-85	36.64638453290293	38.0	38.0	38.0	34.0	38.0
86-87	36.64654553313501	38.0	38.0	38.0	34.0	38.0
88-89	36.593306572265774	38.0	38.0	38.0	34.0	38.0
90-91	36.59254083570518	38.0	38.0	38.0	34.0	38.0
92-93	36.63892968303868	38.0	38.0	38.0	34.0	38.0
94-95	36.53667569321462	38.0	38.0	38.0	34.0	38.0
96-97	36.494550832371274	38.0	38.0	38.0	34.0	38.0
98-99	36.56451452048971	38.0	38.0	38.0	34.0	38.0
100	35.00242837549264	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	40.0
19	96.0
20	180.0
21	412.0
22	1284.0
23	3639.0
24	8472.0
25	17024.0
26	30556.0
27	49183.0
28	74184.0
29	105405.0
30	143355.0
31	190276.0
32	252717.0
33	347257.0
34	524650.0
35	956884.0
36	2718397.0
37	7362956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.19170125461319	22.66595782002756	14.946802483059122	24.195538442300126
2	13.38079415067011	23.237625742269337	40.50567417501364	22.875905932046912
3	17.013335998546644	27.32317564858412	31.478038306028584	24.185450046840653
4	12.268051951952023	19.085320375766923	40.29849450639944	28.348133165881613
5	12.819792509189776	38.480548303545916	36.037986644345196	12.661672542919113
6	28.37406243403928	39.58033988826279	19.536939447798684	12.508658229899241
7	24.82417842727233	33.371121803767565	24.916757051651565	16.887942717308533
8	23.350310773413153	37.42285603413913	22.27970522177695	16.947127970670767
9	24.922123837331885	16.934577454170526	22.286573329971578	35.85672537852601
10-11	23.06586494422564	27.460115841369444	30.204413571347516	19.269605643057396
12-13	23.961444655101612	25.077913303770426	30.154753640209808	20.805888400918153
14-15	21.3515611088132	26.481111356412924	27.530589931046208	24.63673760372767
16-17	20.996238436176704	29.35535387627826	28.333508902696174	21.314898784848857
18-19	21.006236896327817	28.759301754778633	29.771292946749146	20.4631684021444
20-21	21.805584486136894	27.939968254066322	29.482707389385542	20.771739870411242
22-23	21.532222504988514	27.918458352618185	29.305024239084513	21.244294903308788
24-25	21.369362165377748	28.029229832427298	29.376019131366597	21.225388870828354
26-27	21.340295552091785	28.19800281077779	29.27130771563783	21.190393921492596
28-29	21.277570714404234	28.189604545072992	29.36142860350312	21.171396137019656
30-31	21.624853392048124	27.993894519596857	29.275157920628992	21.10609416772603
32-33	21.428787383911416	28.100666630636812	29.238481607934087	21.232064377517684
34-35	21.499185846459532	28.069659187866502	29.25210599910925	21.179048966564714
36-37	21.43388835999528	28.08458451718997	29.2151476351647	21.26637948765005
38-39	21.335133016313105	28.13241416057306	29.342761226985825	21.18969159612801
40-41	21.566259814498927	27.98794069977477	29.23396118680418	21.21183829892212
42-43	21.529418970161128	28.043059567346923	29.04805104736247	21.37947041512948
44-45	21.542304195545473	28.132186448396528	28.952773250871573	21.37273610518643
46-47	21.581375405213574	28.09123982703688	28.7714366454866	21.55594812226295
48-49	21.533041883652135	28.165013384706423	28.80255855743743	21.49938617420401
50-51	21.504581896909926	28.219944619411695	28.753346405200254	21.522127078478125
52-53	21.670748084623085	28.19331592429297	28.666632824915077	21.469303166168867
54-55	21.64219827186266	28.11670600481943	28.68304023549394	21.558055487823967
56-57	21.653758210678156	28.207377342198814	28.599765667140108	21.539098779982922
58-59	21.534015948547495	28.30397027054107	28.730695878409197	21.431317902502244
60-61	21.663934091018934	28.337230816513433	28.617267151714337	21.3815679407533
62-63	21.57115927672785	28.45736125909611	28.567178610561548	21.404300853614487
64-65	21.572563107727806	28.361688170111734	28.55739131186459	21.508357410295872
66-67	21.594919981437442	28.40540990190446	28.435494422218994	21.564175694439104
68-69	21.564867957943136	28.35243016417503	28.495277708387068	21.587424169494764
70-71	21.59412233334775	28.176859265705023	28.525693378058172	21.703325022889057
72-73	21.66367770116409	28.11849962244985	28.518413891888063	21.699408784497997
74-75	21.595086622207884	28.22555675088233	28.432104205379282	21.7472524215305
76-77	21.692902977470236	28.15268700661044	28.38203874817627	21.772371267743058
78-79	21.697632754624273	28.14914063036799	28.35219974647299	21.801026868534745
80-81	21.66756225984052	28.223304292449825	28.32375995665832	21.78537349105133
82-83	21.725056889018347	28.184443819486475	28.393360882295138	21.697138409200036
84-85	21.817029337021054	28.189661082951794	28.3096674103952	21.68364216963195
86-87	21.72021577382834	28.186661953733545	28.358804725985802	21.734317546452314
88-89	21.73509865164957	28.194142287753998	28.332692579579934	21.7380664810165
90-91	21.782389885542027	28.28989145931562	28.297231834052994	21.63048682108936
92-93	21.882664162846243	28.180430648654852	28.297419861393756	21.639485327105145
94-95	21.75834486682832	28.322254074856684	28.26036668663064	21.659034371684356
96-97	21.795464529420858	28.37816038321917	28.374581367778344	21.451793719581627
98-99	22.82735530900658	30.25316706748709	28.63860569289045	18.280871930615877
100	21.628747598274384	31.26199783866273	22.694170776717634	24.415083786345253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1339.0
1	1486.0
2	2472.5
3	3662.0
4	4734.0
5	6489.0
6	8053.5
7	10156.5
8	12291.0
9	13285.0
10	13436.0
11	13392.0
12	14790.0
13	18186.0
14	24478.0
15	31922.5
16	37193.0
17	39415.5
18	38981.5
19	37228.0
20	36073.5
21	36846.0
22	41209.0
23	49975.5
24	63749.0
25	84268.5
26	111253.0
27	141691.0
28	173782.0
29	208306.0
30	242644.5
31	277070.0
32	311266.5
33	342802.0
34	374398.5
35	401254.0
36	427248.0
37	453694.5
38	473109.5
39	487403.5
40	500584.0
41	511307.0
42	517112.5
43	524946.5
44	529986.0
45	529531.5
46	522729.0
47	509909.0
48	496393.5
49	479481.0
50	460747.0
51	436524.5
52	408380.0
53	378478.0
54	346200.5
55	314352.5
56	283106.0
57	250308.0
58	217405.0
59	184533.5
60	149166.0
61	116677.0
62	88985.0
63	65751.0
64	47723.0
65	34460.0
66	24932.0
67	18390.5
68	13358.5
69	9251.0
70	6573.0
71	4724.0
72	3218.5
73	2055.5
74	1249.0
75	743.0
76	435.0
77	242.0
78	130.5
79	74.0
80	41.5
81	25.0
82	16.5
83	12.0
84	11.5
85	10.5
86	8.5
87	5.5
88	3.5
89	4.0
90	3.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.003714719258332448
6	1.5640923192978728E-5
7	0.0
8	0.0
9	7.820461596489364E-6
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.910617365252836E-6
26-27	0.0
28-29	3.129223229006335E-5
30-31	0.0
32-33	2.741401232776804E-5
34-35	0.0
36-37	6.167041265322888E-4
38-39	3.544852726139449E-5
40-41	2.1730272557277344E-4
42-43	9.51399010441961E-5
44-45	6.846203579107663E-4
46-47	0.0
48-49	8.843006725187005E-5
50-51	1.211404794667493E-5
52-53	1.0136297110924217E-4
54-55	0.002121133237213292
56-57	0.0012514391038485474
58-59	1.4795006553776932E-4
60-61	3.676467702706281E-4
62-63	9.966397460429042E-5
64-65	7.725503909564332E-4
66-67	0.0013909537244403847
68-69	3.638767838741995E-4
70-71	6.69035649458047E-4
72-73	2.146264214654237E-4
74-75	1.4690716348710595E-4
76-77	0.0
78-79	0.0015380705995438637
80-81	0.0
82-83	8.674404621117799E-4
84-85	1.2099204333917067E-4
86-87	9.030913448899524E-6
88-89	5.007071578227596E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.559819060611822E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	386.0
22-23	643.0
24-25	836.0
26-27	1636.0
28-29	3405.0
30-31	9833.0
32-33	13545.0
34-35	20269.0
36-37	32707.0
38-39	38414.0
40-41	40990.0
42-43	48094.0
44-45	59754.0
46-47	62515.0
48-49	57986.0
50-51	51865.0
52-53	49551.0
54-55	54829.0
56-57	58098.0
58-59	61135.0
60-61	63969.0
62-63	65781.0
64-65	72739.0
66-67	79872.0
68-69	82138.0
70-71	88506.0
72-73	76554.0
74-75	77478.0
76-77	81717.0
78-79	85092.0
80-81	85784.0
82-83	82414.0
84-85	83735.0
86-87	87640.0
88-89	91670.0
90-91	93374.0
92-93	95679.0
94-95	119115.0
96-97	409716.0
98-99	929095.0
100-101	9268336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.97628726421522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49103786194188	73.42657777941034
2	7.635854582105527	12.671897266251243
3	2.143453253320357	5.3356737865477974
4	0.7956766906734738	2.640891906190491
5	0.36793440194088695	1.526491531491713
6	0.19079048085297037	0.9498651447920293
7	0.11653212798814253	0.6768582329218086
8	0.06325636465078793	0.4199022627642982
9	0.04972403223587308	0.3713324024465012
>10	0.14308026935910703	1.7810143096535735
>50	0.0021668789007245988	0.1176036991941812
>100	4.604752414154527E-4	0.058601721966330275
>500	1.629086703992922E-5	0.009610982765979829
>1k	1.6289921833431935E-5	0.013678973603652094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5640923192978728E-5	0.0	0.0	7.820461596489364E-6
3	0.0	1.5640923192978728E-5	0.0	0.0	7.820461596489364E-6
4	0.0	4.6922769578936183E-5	0.0	1.5640923192978728E-5	7.820461596489364E-6
5	0.0	5.4743231175425544E-5	0.0	1.5640923192978728E-5	7.820461596489364E-6
6	0.0	5.4743231175425544E-5	0.0	1.5640923192978728E-5	7.820461596489364E-6
7	0.0	5.4743231175425544E-5	0.0	2.3461384789468092E-5	7.820461596489364E-6
8	0.0	6.256369277191491E-5	0.0	2.3461384789468092E-5	7.820461596489364E-6
9	0.0	1.0948646235085109E-4	0.0	3.9102307982446816E-5	7.820461596489364E-6
10-11	0.0	1.1730692394734045E-4	0.0	5.8653461973670224E-5	7.820461596489364E-6
12-13	0.0	1.2512738554382982E-4	0.0	7.820461596489363E-5	7.820461596489364E-6
14-15	0.0	1.7596038592101066E-4	0.0	7.820461596489363E-5	7.820461596489364E-6
16-17	0.0	1.7987061671925536E-4	0.0	1.0166600075436173E-4	7.820461596489364E-6
18-19	0.0	1.8378084751750006E-4	0.0	1.2121715474558512E-4	7.820461596489364E-6
20-21	0.0	1.9551153991223408E-4	0.0	1.3685807793856387E-4	7.820461596489364E-6
22-23	0.0	2.0724223230696813E-4	0.0	1.5640923192978726E-4	7.820461596489364E-6
24-25	0.0	2.58075232684149E-4	0.0	1.7987061671925536E-4	7.820461596489364E-6
26-27	0.0	2.815366174736171E-4	0.0	2.111524631052128E-4	7.820461596489364E-6
28-29	0.0	2.815366174736171E-4	0.0	3.32369617850798E-4	7.820461596489364E-6
30-31	0.0	3.55831002640266E-4	0.0	6.99931312885798E-4	7.820461596489364E-6
32-33	0.0	3.753821566314894E-4	0.0	0.0015758230116926066	7.820461596489364E-6
34-35	0.0	4.3403561860515965E-4	0.0	0.002791904789946703	7.820461596489364E-6
36-37	0.0	4.926890805788299E-4	0.0	0.005360926424393459	7.820461596489364E-6
38-39	0.0	5.357016193595214E-4	0.0	0.013834396564189683	1.9551153991223408E-5
40-41	0.0	5.630732349472342E-4	0.0	0.023801574868915376	3.5192077184202136E-5
42-43	3.910230798244682E-6	5.90444850534947E-4	0.0	0.02950660160355437	4.692276957893618E-5
44-45	7.820461596489364E-6	6.725596972980852E-4	0.0	0.036642772810350915	5.4743231175425544E-5
46-47	7.820461596489364E-6	7.194824668770214E-4	0.0	0.04468611756234023	6.256369277191491E-5
48-49	7.820461596489364E-6	7.429438516664895E-4	0.0	0.052706000929540066	6.256369277191491E-5
50-51	7.820461596489364E-6	7.976870828419151E-4	0.0	0.06113254829975735	6.256369277191491E-5
52-53	7.820461596489364E-6	8.015973136401598E-4	0.0	0.0867523804898565	7.429438516664896E-5
54-55	7.820461596489364E-6	8.211484676313831E-4	0.0	0.12011837989127838	8.602507756138299E-5
56-57	7.820461596489364E-6	8.446098524208512E-4	0.0	0.16584070861515343	8.602507756138299E-5
58-59	7.820461596489364E-6	8.446098524208512E-4	0.0	0.2010523369533468	9.384553915787235E-5
60-61	7.820461596489364E-6	8.758916988068087E-4	0.0	0.21690832284022898	1.0166600075436173E-4
62-63	7.820461596489364E-6	8.837121604032981E-4	0.0	0.22873677100491915	1.0166600075436173E-4
64-65	7.820461596489364E-6	9.384553915787236E-4	0.0	0.23295982026702342	1.0166600075436173E-4
66-67	7.820461596489364E-6	9.619167763681917E-4	0.0	0.23713594675954872	1.055762315526064E-4
68-69	7.820461596489364E-6	0.001004929315148883	0.0	0.2411165617121618	1.1730692394734045E-4
70-71	1.1730692394734046E-5	0.0010166600075436172	0.0	0.24463967966138028	1.1730692394734045E-4
72-73	1.5640923192978728E-5	0.0010362111615348406	0.0	0.24788126099312513	1.5640923192978726E-4
74-75	1.5640923192978728E-5	0.001059672546324309	0.0	0.2503212450112298	1.6422969352627664E-4
76-77	1.5640923192978728E-5	0.001106595315903245	0.0	0.2524757821810626	1.6422969352627664E-4
78-79	1.5640923192978728E-5	0.0011105055467014895	0.0	0.25391474711481665	1.6422969352627664E-4
80-81	1.5640923192978728E-5	0.0011144157774997344	0.0	0.254430897580185	1.6422969352627664E-4
82-83	1.5640923192978728E-5	0.0011222362390962237	0.0	0.25485320250639537	1.7987061671925536E-4
84-85	1.5640923192978728E-5	0.001130056700692713	0.0	0.2550799958926936	1.7987061671925536E-4
86-87	1.5640923192978728E-5	0.001153518085482181	0.0	0.2551855721242462	1.7987061671925536E-4
88	1.5640923192978728E-5	0.00122781247064883	0.0	0.2551894823550444	1.7987061671925536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12490	0.0	46.398254	1
GTATCAA	22210	0.0	35.852276	1
TCAACGC	26610	0.0	29.724558	4
ATCAACG	26755	0.0	29.530396	3
CAACGCA	27410	0.0	28.873144	5
AACGCAG	28655	0.0	27.725323	6
TATCAAC	29710	0.0	26.786829	2
ACGCAGA	32425	0.0	24.392605	7
CGCAGAG	33305	0.0	23.748095	8
AGAGTAC	36560	0.0	19.213877	10-11
GCAGAGT	41165	0.0	19.21366	9
GTACATG	30085	0.0	17.483349	1
TACATGG	29935	0.0	17.30495	2
GAGTACT	24455	0.0	17.229315	12-13
CAGAGTA	40425	0.0	16.889908	10-11
ACATGGG	30355	0.0	16.7449	3
AGTACTT	28135	0.0	16.02917	12-13
TATGCCG	4825	0.0	15.131798	42-43
GTGGTAT	7595	0.0	15.085674	1
CATGGGG	22330	0.0	14.600628	4
>>END_MODULE