FastQCFastQC Report
Fri 27 May 2016
SRR2138976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6270711
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63050.049.411681
GTATCAA112550.036.8007241
TCAACGC137550.029.427754
ATCAACG138300.029.2033393
CAACGCA140800.028.6529775
TATCAAC147750.027.699582
AACGCAG145750.027.647786
ACGCAGA168850.023.83887
CGCAGAG175150.022.9813398
AGAGTAC187650.019.39619610-11
CGAGACC31050.019.38991486-87
GCAGAGT212050.018.9188149
GAGTACT120600.018.3606812-13
GTACATG156550.018.3541741
AGACCGA33950.018.04267988-89
GAGACCG33300.017.4595388-89
TACATGG157800.017.44182
CAGAGTA207400.017.11691910-11
GACCGAG34650.017.08741290-91
TCTCCGA34400.017.05325974-75
Produced by FastQC (version 0.11.5)