##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6270711
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.848860998378015	30.0	18.0	32.0	18.0	33.0
2	27.011441924209233	29.0	18.0	32.0	18.0	33.0
3	29.489465548643526	31.0	28.0	33.0	25.0	33.0
4	29.514562543226756	31.0	29.0	33.0	25.0	33.0
5	32.05262305980933	33.0	32.0	33.0	32.0	33.0
6	36.051320655664085	38.0	36.0	38.0	33.0	38.0
7	36.57161125747942	38.0	37.0	38.0	34.0	38.0
8	36.88773665378615	38.0	38.0	38.0	35.0	38.0
9	36.979040494770054	38.0	38.0	38.0	35.0	38.0
10-11	37.084431255084155	38.0	38.0	38.0	36.0	38.0
12-13	37.14185608298644	38.0	38.0	38.0	36.0	38.0
14-15	37.15068283325448	38.0	38.0	38.0	36.0	38.0
16-17	37.12511611522203	38.0	38.0	38.0	36.0	38.0
18-19	37.15839543554152	38.0	38.0	38.0	36.0	38.0
20-21	37.12509818498921	38.0	38.0	38.0	36.0	38.0
22-23	37.15972394095106	38.0	38.0	38.0	36.0	38.0
24-25	37.168841192579194	38.0	38.0	38.0	36.0	38.0
26-27	37.107305788751646	38.0	38.0	38.0	36.0	38.0
28-29	37.097838581309375	38.0	38.0	38.0	36.0	38.0
30-31	36.96567139509271	38.0	38.0	38.0	36.0	38.0
32-33	37.06170756690739	38.0	38.0	38.0	36.0	38.0
34-35	37.07646325307756	38.0	38.0	38.0	36.0	38.0
36-37	36.99945735906252	38.0	38.0	38.0	36.0	38.0
38-39	37.04021736485886	38.0	38.0	38.0	36.0	38.0
40-41	36.95442259006674	38.0	38.0	38.0	36.0	38.0
42-43	36.941909274771426	38.0	38.0	38.0	36.0	38.0
44-45	36.942701510804255	38.0	38.0	38.0	36.0	38.0
46-47	36.9038859356239	38.0	38.0	38.0	35.5	38.0
48-49	36.86990178799937	38.0	38.0	38.0	35.0	38.0
50-51	36.887112204189386	38.0	38.0	38.0	35.0	38.0
52-53	36.79938020494431	38.0	38.0	38.0	35.0	38.0
54-55	36.787911178040844	38.0	38.0	38.0	35.0	38.0
56-57	36.795231093063194	38.0	38.0	38.0	35.0	38.0
58-59	36.81719532222695	38.0	38.0	38.0	35.0	38.0
60-61	36.81310750759786	38.0	38.0	38.0	35.0	38.0
62-63	36.82011817707151	38.0	38.0	38.0	35.0	38.0
64-65	36.7747345964375	38.0	38.0	38.0	35.0	38.0
66-67	36.7813458253122	38.0	38.0	38.0	35.0	38.0
68-69	36.79502018101948	38.0	38.0	38.0	35.0	38.0
70-71	36.71176433960488	38.0	38.0	38.0	34.5	38.0
72-73	36.662024421221375	38.0	38.0	38.0	34.0	38.0
74-75	36.71359392183278	38.0	38.0	38.0	34.5	38.0
76-77	36.69031868540217	38.0	38.0	38.0	34.0	38.0
78-79	36.707663864921926	38.0	38.0	38.0	34.5	38.0
80-81	36.72802146190678	38.0	38.0	38.0	35.0	38.0
82-83	36.596635436150464	38.0	38.0	38.0	34.0	38.0
84-85	36.618954688535325	38.0	38.0	38.0	34.0	38.0
86-87	36.619315012116374	38.0	38.0	38.0	34.0	38.0
88-89	36.560622516057876	38.0	38.0	38.0	34.0	38.0
90-91	36.55934598403896	38.0	38.0	38.0	34.0	38.0
92-93	36.604070194763764	38.0	38.0	38.0	34.0	38.0
94-95	36.4995860914387	38.0	38.0	38.0	34.0	38.0
96-97	36.45865312595147	38.0	38.0	38.0	34.0	38.0
98-99	36.52868055395936	38.0	38.0	38.0	34.0	38.0
100	34.91258057471373	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	13.0
19	39.0
20	71.0
21	181.0
22	633.0
23	1851.0
24	4342.0
25	8718.0
26	15169.0
27	24421.0
28	37077.0
29	53112.0
30	72108.0
31	95955.0
32	127541.0
33	175056.0
34	260407.0
35	467179.0
36	1295868.0
37	3630970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.69200797166382	22.32195041359744	14.889252590336246	24.096789024402497
2	13.572336534086805	23.10930929522984	40.287122146117085	23.031232024566272
3	17.231267714299065	27.113863164799017	31.279148409167632	24.37572071173428
4	12.471632004728013	18.80467781085749	39.99788859668385	28.725801587730643
5	13.053345770467887	38.398260162038916	35.69752119020124	12.850872877291955
6	28.763138464384415	39.243610372669124	19.362735639185992	12.630515523760465
7	25.234012538610056	33.2110186548224	24.64594525246021	16.909023554107343
8	23.73187027754907	37.14899315245113	21.996835765513673	17.122300804486127
9	25.240220006984853	16.798480554833503	22.083448285760305	35.877851152421336
10-11	23.398518923930638	27.247340851779008	29.920825565075475	19.433314659214883
12-13	24.23375116474033	24.93482796448441	29.868120217946576	20.963300652828682
14-15	21.62135202850203	26.265905094334595	27.29294971495258	24.819793162210793
16-17	21.210258294474105	29.131289896791607	28.104516377807876	21.553935430926412
18-19	21.307224970182805	28.540192651200158	29.494980712713435	20.657601665903595
20-21	22.13574101107666	27.69904517258305	29.25581063052035	20.909403185819933
22-23	21.842073871265914	27.610304225707903	29.07664426113743	21.47097764188875
24-25	21.634599206049852	27.74369486490551	29.154598274655203	21.467107654389427
26-27	21.572201437531636	27.908216741599745	29.071676492619947	21.44790532824867
28-29	21.56425258290067	27.84408200996272	29.188893218038807	21.4027721890978
30-31	21.796390044006476	27.76535230193276	29.02743988133881	21.410817772721952
32-33	21.644117667017813	27.884798316419424	28.978510955688254	21.492573060874516
34-35	21.749373072702472	27.836562852263302	29.002520174621516	21.41154390041271
36-37	21.732731016386246	27.780400918918215	28.966583475619068	21.52028458907647
38-39	21.62649078619868	27.92663914826305	29.055666039839284	21.391204025698986
40-41	21.848208094260013	27.797777623661503	28.9129678157803	21.441046466298186
42-43	21.714771465480418	27.89071509679586	28.7829807900388	21.611532647684925
44-45	21.801685620017945	27.906000472978405	28.67559258487546	21.616721322128196
46-47	21.88056894925094	27.87731411088779	28.495072955567835	21.74704398429343
48-49	21.764016193857135	27.958488274684694	28.585147051576886	21.69234847988128
50-51	21.775771601027685	28.08793680870213	28.421345618895266	21.714945971374924
52-53	21.96331188587305	27.995570003296667	28.306448835260216	21.734669275570067
54-55	21.91737419825496	27.90892586095728	28.38840763001209	21.785292310775667
56-57	21.846076182143978	28.002990915457595	28.395210043610362	21.75572285878807
58-59	21.814016977024302	28.030086448725065	28.353532087662504	21.802364486588125
60-61	21.938555942753435	27.97946607575711	28.448067191781114	21.63391078970834
62-63	21.848433642491134	28.027374941234314	28.4372512655957	21.68694015067885
64-65	21.862083396979298	27.93609671124036	28.346460191606916	21.855359700173423
66-67	21.878167995427305	28.006056937640366	28.33358216701672	21.782192899915607
68-69	21.795851875195375	28.085039692927282	28.307482737325245	21.811625694552102
70-71	21.84687263484352	27.89083672995787	28.259353879717064	22.00293675548154
72-73	21.892254365687045	27.916031173968985	28.195785040600423	21.995929419743547
74-75	21.81650911732395	28.010047044261306	28.221112163277905	21.95233167513684
76-77	21.948149971143398	27.858458797450915	28.161860410301028	22.03153082110466
78-79	21.89187732639605	27.85989527795015	28.05871264642059	22.18951474923321
80-81	21.975016919010823	28.039123950736055	28.016723268182343	21.969135862070782
82-83	22.048847916576282	27.91935606006051	28.04075263223978	21.991043391123426
84-85	22.002677182819603	27.890069192128824	28.006194287057994	22.101059337993576
86-87	21.928008092201818	27.972670463311143	28.080386215141573	22.01893522934546
88-89	22.067858771692254	28.005332194817207	28.021421491133257	21.905387542357282
90-91	22.07682862241228	28.050914951877832	28.0083489063507	21.86390751935919
92-93	22.046942917963516	27.902447129292284	28.00435398554637	22.046255967197826
94-95	22.009031232589297	28.044017859229655	28.047750002563536	21.899200905617512
96-97	22.250059684038458	28.032616472382514	28.103310096909645	21.61401374666938
98-99	22.19151933950961	28.828797726374493	27.295633167048923	21.684049767066973
100	20.26611730795309	28.501866148213324	28.492937492313075	22.739079051520513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	672.0
1	746.5
2	1224.5
3	1811.5
4	2331.5
5	3172.0
6	3933.0
7	4965.5
8	6006.0
9	6515.5
10	6629.0
11	6695.0
12	7440.0
13	8871.0
14	11734.0
15	15393.0
16	17691.0
17	18462.5
18	18281.0
19	17304.0
20	16305.5
21	16307.5
22	17939.0
23	21625.5
24	27754.5
25	36766.0
26	48253.5
27	61035.5
28	75302.0
29	91260.0
30	106866.5
31	122223.5
32	138497.0
33	154481.5
34	169751.0
35	183595.0
36	198303.0
37	212372.5
38	221463.0
39	228908.0
40	236674.5
41	243155.0
42	245714.0
43	247251.5
44	248322.5
45	249068.0
46	248869.5
47	245400.5
48	240938.0
49	235029.5
50	227844.0
51	218749.0
52	207762.5
53	194166.5
54	179260.5
55	164550.5
56	148651.0
57	131866.0
58	116066.5
59	99580.5
60	81091.5
61	63571.0
62	48584.5
63	36113.5
64	25914.5
65	18602.0
66	13571.0
67	9946.0
68	7244.5
69	5178.0
70	3775.5
71	2642.5
72	1683.0
73	1002.0
74	576.0
75	321.5
76	192.0
77	111.0
78	62.0
79	36.5
80	21.0
81	13.5
82	12.0
83	11.0
84	8.0
85	3.0
86	1.0
87	1.5
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0038592114993020728
6	1.5947154955793688E-5
7	0.0
8	0.0
9	1.5947154955793688E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.5948535988190426E-5
26-27	0.0
28-29	6.380728668047616E-5
30-31	0.0
32-33	7.185230614766735E-5
34-35	0.0
36-37	6.001049463530182E-4
38-39	4.008700483529452E-5
40-41	2.0894051648327717E-4
42-43	7.251043284136078E-5
44-45	6.95076209448769E-4
46-47	0.0
48-49	1.3031957373119032E-4
50-51	8.174388480128593E-6
52-53	8.20065359209129E-5
54-55	0.0022871511713957356
56-57	0.0013045452668950996
58-59	9.93814827783485E-5
60-61	3.323813654043681E-4
62-63	1.0849680184811782E-4
64-65	8.04825060374455E-4
66-67	0.0014238925633461577
68-69	3.303850281704065E-4
70-71	7.667265224505193E-4
72-73	1.1994455134740568E-4
74-75	1.5506801024482654E-4
76-77	0.0
78-79	0.0014301321163025294
80-81	0.0
82-83	7.157036152928721E-4
84-85	1.0672433575440455E-4
86-87	0.0
88-89	7.214925877909927E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.809754606335116E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	23.0
20-21	149.0
22-23	287.0
24-25	403.0
26-27	703.0
28-29	1325.0
30-31	3873.0
32-33	5031.0
34-35	7280.0
36-37	11872.0
38-39	14196.0
40-41	15314.0
42-43	18292.0
44-45	23193.0
46-47	24333.0
48-49	22499.0
50-51	20189.0
52-53	19224.0
54-55	21152.0
56-57	23279.0
58-59	14255.0
60-61	26409.0
62-63	26398.0
64-65	28812.0
66-67	31805.0
68-69	32847.0
70-71	33490.0
72-73	31941.0
74-75	33454.0
76-77	35640.0
78-79	37286.0
80-81	37691.0
82-83	36983.0
84-85	37277.0
86-87	39457.0
88-89	41593.0
90-91	42909.0
92-93	44587.0
94-95	57245.0
96-97	217169.0
98-99	77294.0
100-101	5073552.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.11307315542456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.40647520913042	80.5410543698161
2	6.283932973402955	11.073932915784782
3	1.3584313844488545	3.5908669186369973
4	0.475971406346333	1.67757213389139
5	0.1995171009119301	0.879003245420556
6	0.10009158455697516	0.5291626267386669
7	0.05329783470815567	0.32873652060658126
8	0.034186634406714	0.24098315347332364
9	0.020439589377155017	0.16208955306504888
>10	0.06663511959310055	0.8765999886672987
>50	7.59342817053518E-4	0.044947492904329284
>100	2.3617846335192177E-4	0.0436638004316776
>500	2.5641837053394677E-5	0.011387280563191217
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1894309911587376E-5	0.0	0.0	0.0
3	0.0	4.7841464867381065E-5	0.0	0.0	0.0
4	0.0	9.568292973476213E-5	0.0	0.0	0.0
5	0.0	9.568292973476213E-5	0.0	0.0	0.0
6	1.5947154955793688E-5	9.568292973476213E-5	0.0	1.5947154955793688E-5	0.0
7	1.5947154955793688E-5	9.568292973476213E-5	0.0	1.5947154955793688E-5	0.0
8	1.5947154955793688E-5	9.568292973476213E-5	0.0	1.5947154955793688E-5	0.0
9	1.5947154955793688E-5	1.7541870451373058E-4	0.0	9.568292973476213E-5	0.0
10-11	2.3920732433690532E-5	1.9136585946952426E-4	0.0	1.435243946021432E-4	0.0
12-13	3.1894309911587376E-5	1.9136585946952426E-4	0.0	1.8339228199162742E-4	0.0
14-15	3.1894309911587376E-5	2.3920732433690533E-4	0.0	2.0731301442531797E-4	0.0
16-17	5.581504234527791E-5	2.3920732433690533E-4	0.0	2.2326016938111165E-4	0.0
18-19	7.17621973010716E-5	2.55154479292699E-4	0.0	2.631280567705959E-4	1.5947154955793688E-5
20-21	7.973577477896845E-5	2.55154479292699E-4	0.0	3.10969521637977E-4	1.5947154955793688E-5
22-23	7.973577477896845E-5	2.8704878920428643E-4	0.0	3.348902540716675E-4	1.5947154955793688E-5
24-25	8.770935225686529E-5	2.9502236668218324E-4	0.0	3.4286383154956434E-4	1.5947154955793688E-5
26-27	9.568292973476213E-5	3.029959441600801E-4	0.0	3.5083740902746115E-4	1.5947154955793688E-5
28-29	9.568292973476213E-5	3.10969521637977E-4	0.0	3.907052964169454E-4	1.5947154955793688E-5
30-31	9.568292973476213E-5	3.5083740902746115E-4	0.0	6.059918883201602E-4	2.3920732433690532E-5
32-33	1.0365650721265898E-4	3.5083740902746115E-4	0.0	0.0011960366216845267	3.1894309911587376E-5
34-35	1.275772396463495E-4	4.5449391624012017E-4	0.0	0.001993394369474211	3.1894309911587376E-5
36-37	1.4352439460214321E-4	5.581504234527791E-4	0.0	0.0033568761181945713	3.1894309911587376E-5
38-39	1.4352439460214321E-4	5.980183108422634E-4	0.0	0.009289217761749824	3.1894309911587376E-5
40-41	1.7541870451373058E-4	6.618069306654381E-4	0.0	0.015213585827827178	3.1894309911587376E-5
42-43	1.9136585946952426E-4	7.096483955328192E-4	0.0	0.01890535220009342	3.1894309911587376E-5
44-45	1.9136585946952426E-4	8.451992126570656E-4	0.0	0.023713419419265216	3.1894309911587376E-5
46-47	1.9136585946952426E-4	9.408821423918277E-4	0.0	0.028944086244765545	3.1894309911587376E-5
48-49	1.9136585946952426E-4	9.408821423918277E-4	0.0	0.03375215346393734	3.1894309911587376E-5
50-51	1.993394369474211E-4	0.001068459382038177	0.0	0.03930973696603145	4.7841464867381065E-5
52-53	2.0731301442531797E-4	0.0010923801144718677	0.0	0.05980980466170423	5.581504234527791E-5
54-55	2.0731301442531797E-4	0.0011242744243834552	0.0	0.08446410622336128	6.378861982317475E-5
56-57	2.0731301442531797E-4	0.0011641423117729392	0.0	0.11502682869613988	6.378861982317475E-5
58-59	2.0731301442531797E-4	0.001180089466728733	0.0	0.22243091732341036	6.378861982317475E-5
60-61	2.0731301442531797E-4	0.0012119837766403204	0.0	0.3144141071084284	6.378861982317475E-5
62-63	2.0731301442531797E-4	0.0012438780865519077	0.0	0.3189191783834401	6.378861982317475E-5
64-65	2.0731301442531797E-4	0.0013475345937645667	0.0	0.32253120898092735	6.378861982317475E-5
66-67	2.0731301442531797E-4	0.0013874024811540509	0.0	0.326191081043282	6.378861982317475E-5
68-69	2.0731301442531797E-4	0.0014033496361098446	0.0	0.32939645918939653	6.378861982317475E-5
70-71	2.0731301442531797E-4	0.0014033496361098446	0.0	0.3324423657859531	6.378861982317475E-5
72-73	2.0731301442531797E-4	0.0014033496361098446	0.0	0.3349939105788801	6.378861982317475E-5
74-75	2.0731301442531797E-4	0.0014272703685435351	0.0	0.33718664438530177	6.378861982317475E-5
76-77	2.2326016938111165E-4	0.0015468740307119878	0.0	0.33969832129083927	6.378861982317475E-5
78-79	2.3920732433690533E-4	0.0016186362280130595	0.0	0.3411016709269491	6.378861982317475E-5
80-81	2.3920732433690533E-4	0.0016266098054909563	0.0	0.34147642906841025	7.17621973010716E-5
82-83	2.3920732433690533E-4	0.0016266098054909563	0.0	0.3419309229846504	7.973577477896845E-5
84-85	2.55154479292699E-4	0.0016266098054909563	0.0	0.34213823599907567	7.973577477896845E-5
86-87	2.870487892042864E-4	0.0016266098054909563	0.0	0.3422578396612442	7.973577477896845E-5
88	3.029959441600801E-4	0.001690398425314131	0.0	0.3422578396612442	7.973577477896845E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6305	0.0	49.41168	1
GTATCAA	11255	0.0	36.800724	1
TCAACGC	13755	0.0	29.42775	4
ATCAACG	13830	0.0	29.203339	3
CAACGCA	14080	0.0	28.652977	5
TATCAAC	14775	0.0	27.69958	2
AACGCAG	14575	0.0	27.64778	6
ACGCAGA	16885	0.0	23.8388	7
CGCAGAG	17515	0.0	22.981339	8
AGAGTAC	18765	0.0	19.396196	10-11
CGAGACC	3105	0.0	19.389914	86-87
GCAGAGT	21205	0.0	18.918814	9
GAGTACT	12060	0.0	18.36068	12-13
GTACATG	15655	0.0	18.354174	1
AGACCGA	3395	0.0	18.042679	88-89
GAGACCG	3330	0.0	17.45953	88-89
TACATGG	15780	0.0	17.4418	2
CAGAGTA	20740	0.0	17.116919	10-11
GACCGAG	3465	0.0	17.087412	90-91
TCTCCGA	3440	0.0	17.053259	74-75
>>END_MODULE