FastQCFastQC Report
Fri 27 May 2016
SRR2138975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5543698
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.043.8767551
GTATCAA94600.036.742861
ATCAACG113250.030.098013
TCAACGC113850.029.978784
CAACGCA115450.029.4856175
AACGCAG119850.028.5148756
TATCAAC126450.027.4171982
ATCTCGT39900.025.21808494
ACGCAGA140300.024.2626747
CGCAGAG144350.023.6440818
TATCTCG43350.022.65515394
AGAGTAC158550.019.60295510-11
GCAGAGT177500.019.203039
GTACATG137750.018.49351
TACATGG136900.018.182432
ACATGGG134950.018.0131133
CAGAGTA176600.016.8628869
CGCTATC59800.016.82611394
GAGTACT101850.016.60102312-13
AGTACTT118150.016.132812-13
Produced by FastQC (version 0.11.5)