##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5543698
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.033959642101717	30.0	18.0	32.0	18.0	33.0
2	27.168802665657473	29.0	25.0	32.0	18.0	33.0
3	29.57503312770645	31.0	28.0	33.0	25.0	33.0
4	29.583407140865177	31.0	29.0	33.0	25.0	33.0
5	32.09124090092931	33.0	32.0	33.0	32.0	33.0
6	36.08223554024768	38.0	36.0	38.0	33.0	38.0
7	36.58575719673041	38.0	37.0	38.0	34.0	38.0
8	36.90538716214339	38.0	38.0	38.0	35.0	38.0
9	36.99405505133938	38.0	38.0	38.0	35.0	38.0
10-11	37.093403807350256	38.0	38.0	38.0	36.0	38.0
12-13	37.14792968520291	38.0	38.0	38.0	36.0	38.0
14-15	37.15662469348078	38.0	38.0	38.0	36.0	38.0
16-17	37.130440096123564	38.0	38.0	38.0	36.0	38.0
18-19	37.1648277918458	38.0	38.0	38.0	36.0	38.0
20-21	37.1307869907542	38.0	38.0	38.0	36.0	38.0
22-23	37.16621588956389	38.0	38.0	38.0	36.0	38.0
24-25	37.17363724890022	38.0	38.0	38.0	36.0	38.0
26-27	37.11085828812367	38.0	38.0	38.0	36.0	38.0
28-29	37.10002505344836	38.0	38.0	38.0	36.0	38.0
30-31	36.96746017019436	38.0	38.0	38.0	36.0	38.0
32-33	37.06244914312935	38.0	38.0	38.0	36.0	38.0
34-35	37.075566281553485	38.0	38.0	38.0	36.0	38.0
36-37	36.998545090611	38.0	38.0	38.0	36.0	38.0
38-39	37.03902143243353	38.0	38.0	38.0	36.0	38.0
40-41	36.95615989817921	38.0	38.0	38.0	36.0	38.0
42-43	36.94735344356204	38.0	38.0	38.0	36.0	38.0
44-45	36.94940054296573	38.0	38.0	38.0	36.0	38.0
46-47	36.909084523182294	38.0	38.0	38.0	36.0	38.0
48-49	36.877454486520236	38.0	38.0	38.0	35.0	38.0
50-51	36.8969607012386	38.0	38.0	38.0	35.0	38.0
52-53	36.80842619069997	38.0	38.0	38.0	35.0	38.0
54-55	36.79681743462457	38.0	38.0	38.0	35.0	38.0
56-57	36.804498992102054	38.0	38.0	38.0	35.0	38.0
58-59	36.824229015293355	38.0	38.0	38.0	35.0	38.0
60-61	36.819915044192214	38.0	38.0	38.0	35.0	38.0
62-63	36.826271370512266	38.0	38.0	38.0	35.0	38.0
64-65	36.78148412813395	38.0	38.0	38.0	35.0	38.0
66-67	36.78352114386666	38.0	38.0	38.0	35.0	38.0
68-69	36.79766464010302	38.0	38.0	38.0	35.0	38.0
70-71	36.71526447564961	38.0	38.0	38.0	35.0	38.0
72-73	36.66967317062658	38.0	38.0	38.0	34.0	38.0
74-75	36.71906823138217	38.0	38.0	38.0	34.5	38.0
76-77	36.69592548705327	38.0	38.0	38.0	34.0	38.0
78-79	36.71083243821677	38.0	38.0	38.0	34.5	38.0
80-81	36.73081951172219	38.0	38.0	38.0	35.0	38.0
82-83	36.598138855674975	38.0	38.0	38.0	34.0	38.0
84-85	36.624595762934675	38.0	38.0	38.0	34.0	38.0
86-87	36.62712646759806	38.0	38.0	38.0	34.0	38.0
88-89	36.570429406136185	38.0	38.0	38.0	34.0	38.0
90-91	36.569787171039216	38.0	38.0	38.0	34.0	38.0
92-93	36.61651785262278	38.0	38.0	38.0	34.0	38.0
94-95	36.505242834940816	38.0	38.0	38.0	34.0	38.0
96-97	36.44655409898999	38.0	38.0	38.0	34.0	38.0
98-99	36.5225187181	38.0	38.0	38.0	34.0	38.0
100	34.9378466982057	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	18.0
19	29.0
20	70.0
21	189.0
22	570.0
23	1648.0
24	3922.0
25	7843.0
26	13683.0
27	22047.0
28	33124.0
29	46756.0
30	63180.0
31	83308.0
32	110606.0
33	152522.0
34	225581.0
35	405591.0
36	1128309.0
37	3244700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.273693841186876	22.635360006984506	14.9061510926461	24.18479505918252
2	13.244444412376	23.388521524801675	40.78059446961216	22.586439593210166
3	16.932289601634142	27.649161263835083	31.49810830243639	23.92044083209439
4	12.176673404647945	19.172472959385594	40.61581276613553	28.035040869830937
5	12.68975875468974	38.707520863225774	36.07861551377599	12.524104868308497
6	27.92870390847409	39.848707487312616	19.779486544901978	12.443102059311311
7	24.551756706760848	33.50774762762106	25.2055622809111	16.73493338470699
8	23.16987685837143	37.54986292543353	22.482862522453424	16.797397693741615
9	24.987364030291694	16.969503028483874	22.45365818989418	35.58947475133024
10-11	22.992702704945327	27.605526130752434	30.22510425351453	19.17666691078771
12-13	23.82461129736865	25.2290709198084	30.287571581280222	20.658746201542723
14-15	21.276853464961476	26.552555712811195	27.66696526398083	24.5036255582465
16-17	20.858865327801045	29.438318970477827	28.452181197460614	21.250634504260514
18-19	20.91549720060508	28.85780394242255	29.87036811889825	20.356330738074117
20-21	21.704800107221832	28.057215764279363	29.567025100984967	20.670959027513838
22-23	21.462537929646086	27.97252884330747	29.42003832605723	21.144894900989215
24-25	21.263268413418867	28.08736406866742	29.462570626564972	21.18679689134874
26-27	21.201883329717557	28.281337598894428	29.382279940301387	21.134499131086624
28-29	21.220393243175824	28.286931810236354	29.401963157439365	21.090711789148457
30-31	21.413201209747037	28.053850006132485	29.427066858008686	21.10588192611179
32-33	21.290630862038647	28.2168709669874	29.286606237084946	21.205891933889006
34-35	21.356854259345653	28.21659603982896	29.32381284947132	21.102736851354063
36-37	21.388013446434012	28.173411383120623	29.292206472556064	21.146368697889297
38-39	21.267811940869088	28.20653019565343	29.346635865841584	21.179021997635893
40-41	21.446584888737693	28.09318369758685	29.281981206214002	21.178250207461453
42-43	21.390465441265302	28.147571862331766	29.12930636898815	21.332656327414785
44-45	21.400712018844086	28.243283129548324	29.05365772327098	21.30234712833661
46-47	21.445036018297543	28.15610819759841	28.92342481034126	21.475430973762784
48-49	21.401367608150736	28.306862779875715	28.874978059577515	21.416791552396035
50-51	21.333378219254655	28.41180785816838	28.799180141383253	21.45563378119371
52-53	21.462563547268665	28.259973840655086	28.73302029669176	21.544442315384494
54-55	21.535432676857592	28.129212781391093	28.84504044084806	21.49031410090326
56-57	21.51893805207998	28.024415775762616	28.807012628520244	21.649633543637155
58-59	21.299873299517476	28.060423301497895	28.94631559838869	21.693387800595946
60-61	21.501730536088697	28.172097925097518	28.985369362096442	21.340802176717343
62-63	21.255497924292442	28.299706262945406	28.963100247064617	21.481695565697535
64-65	21.16677136682009	28.428309718467492	28.798754650886078	21.60616426382634
66-67	21.259958077816577	28.690783687411447	28.555487735904418	21.493770498867555
68-69	21.243761972074815	28.6924614324384	28.454049050889985	21.609727544596797
70-71	21.262406026475183	28.335408957223585	28.453679814708355	21.948505201592877
72-73	21.396046328420574	28.081084847006572	28.467970318333833	22.054898506239017
74-75	21.403764171246078	28.165392749560787	28.517896320222697	21.91294675897044
76-77	21.599336717457117	28.0411448057066	28.25006788976768	22.1094505870686
78-79	21.66244224019581	28.119363049837375	27.991212355610916	22.2269823543559
80-81	21.64339907708341	28.306227587077192	27.990399864972776	22.059973470866623
82-83	21.619112306218604	28.2031112519618	28.08088434946224	22.096892092357354
84-85	21.70285623561929	28.372988905981693	28.02724050377131	21.896914354627704
86-87	21.820159068134377	28.36132606645797	28.05051441397871	21.76800045142894
88-89	21.80215727082049	28.375262182456613	28.15233334757409	21.67024719914881
90-91	21.788033593762417	28.216319709902816	28.12882292104112	21.866823775293643
92-93	21.811794176974907	27.979397097897913	28.237988172607213	21.970820552519964
94-95	21.407490390179877	28.301674161202893	28.468921239648537	21.821914208968693
96-97	21.49247571929457	28.24780525279075	28.62768315220353	21.632035875711146
98-99	22.602468990689403	30.129932615971413	28.9749264821988	18.292671911140392
100	21.496995463782156	31.189947404275348	22.838687561641287	24.474369570301207
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	510.0
1	606.5
2	1093.5
3	1669.5
4	2209.0
5	3033.5
6	3685.0
7	4567.0
8	5523.0
9	5832.0
10	5733.0
11	5645.5
12	6186.0
13	7605.5
14	10269.0
15	13425.0
16	15599.5
17	16314.0
18	15829.5
19	14903.0
20	14216.0
21	14301.0
22	15904.5
23	19622.0
24	25730.5
25	34184.5
26	45215.5
27	58179.5
28	72276.5
29	87691.0
30	102835.5
31	117380.5
32	133031.0
33	148463.5
34	162432.0
35	174754.0
36	187213.0
37	199037.5
38	206632.5
39	212207.0
40	218297.5
41	222667.5
42	224107.5
43	223573.5
44	223313.5
45	225187.0
46	222876.0
47	216529.0
48	210335.0
49	202883.5
50	193814.0
51	183015.0
52	171088.0
53	158246.5
54	144805.5
55	131188.0
56	117209.0
57	102828.5
58	89338.0
59	74984.5
60	59763.0
61	46427.0
62	35095.5
63	25779.0
64	18531.5
65	13246.5
66	9585.5
67	6940.0
68	4895.0
69	3444.0
70	2440.5
71	1721.0
72	1186.5
73	764.0
74	492.5
75	308.0
76	164.5
77	86.0
78	52.5
79	34.5
80	26.5
81	19.5
82	13.0
83	7.5
84	3.5
85	2.5
86	2.0
87	1.0
88	0.0
89	1.0
90	1.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0038241621387023607
6	0.0
7	1.8038500654256417E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.0199947124791E-6
26-27	0.0
28-29	4.510971042452929E-5
30-31	0.0
32-33	3.612443676358642E-5
34-35	0.0
36-37	6.698730653907455E-4
38-39	5.440557287164039E-5
40-41	2.544139225474975E-4
42-43	8.199867581249528E-5
44-45	7.769678218319549E-4
46-47	0.0
48-49	1.0135198013206347E-4
50-51	0.0
52-53	1.1113495367478375E-4
54-55	0.0024833596371774506
56-57	0.0013164868750430525
58-59	1.6696719215261618E-4
60-61	4.3753108099780174E-4
62-63	1.2159137292373379E-4
64-65	8.740600682274372E-4
66-67	0.0016159274885141574
68-69	3.8977867226173355E-4
70-71	7.558103157201466E-4
72-73	2.0221963980252577E-4
74-75	1.6468028340120583E-4
76-77	9.747273663188222E-6
78-79	0.001658277808828239
80-81	9.88196780020084E-6
82-83	9.455574872576175E-4
84-85	1.5034695566959873E-4
86-87	0.0
88-89	8.137567201047143E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.64538227361083E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	126.0
22-23	234.0
24-25	326.0
26-27	649.0
28-29	1356.0
30-31	3509.0
32-33	4678.0
34-35	6906.0
36-37	11047.0
38-39	8313.0
40-41	14655.0
42-43	16838.0
44-45	21128.0
46-47	22073.0
48-49	20501.0
50-51	11734.0
52-53	2979.0
54-55	2794.0
56-57	2834.0
58-59	13520.0
60-61	25442.0
62-63	25312.0
64-65	28045.0
66-67	31260.0
68-69	32221.0
70-71	35085.0
72-73	30814.0
74-75	31431.0
76-77	33406.0
78-79	35582.0
80-81	36458.0
82-83	35339.0
84-85	34857.0
86-87	36990.0
88-89	39450.0
90-91	40540.0
92-93	41676.0
94-95	52110.0
96-97	184968.0
98-99	435731.0
100-101	4130754.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.32138587189225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.03583910115204	77.7201840935015
2	7.238179075816439	12.496192980268146
3	1.6160575801612433	4.185009899048853
4	0.5509899006947656	1.9024884731755385
5	0.22857532873747946	0.9865469576371294
6	0.11565575830680896	0.5990139204665018
7	0.07058590179440019	0.4265151006136938
8	0.03657227603475762	0.2525575641447736
9	0.026407420664460463	0.20515726341524457
>10	0.07930531197356783	1.0206067453098475
>50	0.0011082772373775447	0.06647397768527154
>100	6.621853505290932E-4	0.11083493316753211
>500	6.188207610931411E-5	0.028418091565948923
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8038500654256417E-5	0.0
2	0.0	0.0	0.0	1.8038500654256417E-5	0.0
3	0.0	0.0	0.0	1.8038500654256417E-5	0.0
4	0.0	0.0	0.0	1.8038500654256417E-5	0.0
5	0.0	0.0	0.0	1.8038500654256417E-5	0.0
6	1.8038500654256418E-4	0.0	0.0	1.8038500654256417E-5	0.0
7	1.8038500654256418E-4	0.0	0.0	1.8038500654256417E-5	0.0
8	1.8038500654256418E-4	0.0	0.0	3.6077001308512835E-5	0.0
9	1.8038500654256418E-4	3.6077001308512835E-5	0.0	3.6077001308512835E-5	0.0
10-11	1.984235071968206E-4	3.6077001308512835E-5	0.0	7.215400261702567E-5	0.0
12-13	1.984235071968206E-4	3.6077001308512835E-5	0.0	8.117325294415388E-5	0.0
14-15	2.074427575239488E-4	3.6077001308512835E-5	0.0	9.019250327128209E-5	0.0
16-17	3.6077001308512836E-4	5.4115501962769256E-5	0.0	9.019250327128209E-5	0.0
18-19	3.6077001308512836E-4	8.117325294415388E-5	0.0	1.0823100392553851E-4	0.0
20-21	3.6077001308512836E-4	1.0823100392553851E-4	0.0	1.1725025425266672E-4	0.0
22-23	3.6077001308512836E-4	1.4430800523405134E-4	0.0	1.4430800523405134E-4	0.0
24-25	3.6978926341225656E-4	1.4430800523405134E-4	0.0	1.4430800523405134E-4	0.0
26-27	3.968470143936412E-4	1.4430800523405134E-4	0.0	1.7136575621543596E-4	0.0
28-29	4.1488551504789765E-4	1.5332725556117956E-4	0.0	2.5253900915958984E-4	0.0
30-31	4.1488551504789765E-4	1.8038500654256418E-4	0.0	4.8703951766492333E-4	0.0
32-33	4.2390476537502585E-4	1.984235071968206E-4	0.0	0.0014430800523405134	0.0
34-35	4.690010170106669E-4	2.074427575239488E-4	0.0	0.0030394873602422063	0.0
36-37	5.050780183191797E-4	2.3450050850533344E-4	0.0	0.007107169257777029	0.0
38-39	5.050780183191797E-4	2.3450050850533344E-4	0.0	0.06459587084289223	0.0
40-41	5.231165189734362E-4	2.886160104681027E-4	0.0	0.12613421582488799	0.0
42-43	5.231165189734362E-4	2.886160104681027E-4	0.0	0.13839137701945525	0.0
44-45	5.231165189734362E-4	2.886160104681027E-4	0.0	0.1556993183972143	0.0
46-47	5.231165189734362E-4	3.066545111223591E-4	0.0	0.17596557388227135	0.0
48-49	5.231165189734362E-4	3.3371226210374377E-4	0.0	0.19597929035816886	0.0
50-51	5.682127706090772E-4	4.1488551504789765E-4	0.0	0.21806743440930584	0.0
52-53	5.952705215904619E-4	4.1488551504789765E-4	0.0	0.45434473522908353	0.0
54-55	5.952705215904619E-4	4.2390476537502585E-4	0.0	0.7731481765420845	0.0
56-57	5.952705215904619E-4	4.3292401570215405E-4	0.0	1.1302653932447257	0.0
58-59	5.952705215904619E-4	4.4194326602928224E-4	0.0	1.5167492890124967	0.0
60-61	6.223282725718465E-4	4.599817666835387E-4	0.0	1.6265045462433199	0.0
62-63	6.313475228989746E-4	4.690010170106669E-4	0.0	1.63165453818011	0.0
64-65	6.313475228989746E-4	5.050780183191797E-4	0.0	1.6358575088325518	0.0
66-67	6.674245242074875E-4	5.050780183191797E-4	0.0	1.6393750164601317	0.0
68-69	6.854630248617439E-4	5.140972686463079E-4	0.0	1.642766254583132	0.0
70-71	7.395785268245131E-4	5.231165189734362E-4	0.0	1.6456704531884672	0.0
72-73	7.576170274787696E-4	5.501742699548207E-4	0.0	1.6481597662787548	0.0
74-75	7.576170274787696E-4	5.772320209362054E-4	0.0	1.6506039831174064	0.0
76-77	7.576170274787696E-4	6.133090222447183E-4	0.0	1.6526333144410104	0.0
78-79	7.75655528133026E-4	6.313475228989746E-4	0.0	1.6540763944933508	0.0
80-81	7.75655528133026E-4	6.313475228989746E-4	0.0	1.6544822607580716	0.0
82-83	7.75655528133026E-4	6.313475228989746E-4	0.0	1.6548340115208295	0.0
84-85	7.75655528133026E-4	6.403667732261028E-4	0.0	1.6550324350280263	0.0
86-87	7.75655528133026E-4	6.854630248617439E-4	0.0	1.6551767430332605	0.0
88	8.297710300957953E-4	7.215400261702567E-4	0.0	1.6551767430332605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5530	0.0	43.876755	1
GTATCAA	9460	0.0	36.74286	1
ATCAACG	11325	0.0	30.09801	3
TCAACGC	11385	0.0	29.97878	4
CAACGCA	11545	0.0	29.485617	5
AACGCAG	11985	0.0	28.514875	6
TATCAAC	12645	0.0	27.417198	2
ATCTCGT	3990	0.0	25.218084	94
ACGCAGA	14030	0.0	24.262674	7
CGCAGAG	14435	0.0	23.644081	8
TATCTCG	4335	0.0	22.655153	94
AGAGTAC	15855	0.0	19.602955	10-11
GCAGAGT	17750	0.0	19.20303	9
GTACATG	13775	0.0	18.4935	1
TACATGG	13690	0.0	18.18243	2
ACATGGG	13495	0.0	18.013113	3
CAGAGTA	17660	0.0	16.862886	9
CGCTATC	5980	0.0	16.826113	94
GAGTACT	10185	0.0	16.601023	12-13
AGTACTT	11815	0.0	16.1328	12-13
>>END_MODULE