FastQCFastQC Report
Fri 27 May 2016
SRR2138974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6591047
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68550.047.4367681
GTATCAA125950.037.9637221
ATCAACG146700.031.8788993
TCAACGC148300.031.59394
CAACGCA150600.031.0533475
AACGCAG157050.029.832986
CTTATAC74000.029.3544751
TATCAAC161650.029.0658072
ACGCAGA174300.026.8052647
CGCAGAG179050.026.0697368
TATACAC105350.023.4403213
TTATACA106150.021.5343282
GCAGAGT217700.021.5216779
GTACATG162050.019.8508191
AGAGTAC196550.019.65707810-11
TACATGG160950.019.4433752
GAGTACT126150.019.43637812-13
ACACATC114650.018.7174726
ACATGGG162150.018.5447563
CAGAGTA212450.018.47393210-11
Produced by FastQC (version 0.11.5)