##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6591047
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.503247966521858	32.0	28.0	33.0	18.0	34.0
2	31.585423378106697	33.0	31.0	33.0	28.0	34.0
3	32.35790216637812	33.0	33.0	34.0	30.0	34.0
4	32.61615036275724	33.0	33.0	34.0	31.0	34.0
5	32.96227109289313	33.0	33.0	34.0	32.0	34.0
6	36.50974526505425	38.0	37.0	38.0	34.0	38.0
7	36.63333033431562	38.0	37.0	38.0	34.0	38.0
8	36.681885897642665	38.0	38.0	38.0	34.0	38.0
9	36.92798913435149	38.0	38.0	38.0	35.0	38.0
10-11	37.059021351236005	38.0	38.0	38.0	36.0	38.0
12-13	37.11927573874075	38.0	38.0	38.0	36.0	38.0
14-15	37.12135090221629	38.0	38.0	38.0	36.0	38.0
16-17	37.07783543342962	38.0	38.0	38.0	36.0	38.0
18-19	37.12123134609721	38.0	38.0	38.0	36.0	38.0
20-21	37.0947526106974	38.0	38.0	38.0	36.0	38.0
22-23	37.13933386660235	38.0	38.0	38.0	36.0	38.0
24-25	37.14586880082429	38.0	38.0	38.0	36.0	38.0
26-27	37.08013348931766	38.0	38.0	38.0	36.0	38.0
28-29	37.067606265430285	38.0	38.0	38.0	36.0	38.0
30-31	36.93719417655925	38.0	38.0	38.0	36.0	38.0
32-33	37.034803750631674	38.0	38.0	38.0	36.0	38.0
34-35	37.047115268209	38.0	38.0	38.0	36.0	38.0
36-37	36.97401786402732	38.0	38.0	38.0	36.0	38.0
38-39	36.99818611910266	38.0	38.0	38.0	36.0	38.0
40-41	36.93145212189056	38.0	38.0	38.0	36.0	38.0
42-43	36.91554963433975	38.0	38.0	38.0	35.5	38.0
44-45	36.91236809165527	38.0	38.0	38.0	36.0	38.0
46-47	36.86763183397943	38.0	38.0	38.0	35.0	38.0
48-49	36.83459899833729	38.0	38.0	38.0	35.0	38.0
50-51	36.85107168158021	38.0	38.0	38.0	35.0	38.0
52-53	36.75926238742889	38.0	38.0	38.0	35.0	38.0
54-55	36.74353338468306	38.0	38.0	38.0	35.0	38.0
56-57	36.74435514466412	38.0	38.0	38.0	35.0	38.0
58-59	36.7601240064845	38.0	38.0	38.0	35.0	38.0
60-61	36.75167951543363	38.0	38.0	38.0	35.0	38.0
62-63	36.755419319474186	38.0	38.0	38.0	35.0	38.0
64-65	36.70702553239479	38.0	38.0	38.0	35.0	38.0
66-67	36.71054192346525	38.0	38.0	38.0	35.0	38.0
68-69	36.71907502719341	38.0	38.0	38.0	35.0	38.0
70-71	36.630721095611506	38.0	38.0	38.0	34.0	38.0
72-73	36.585431820667516	38.0	38.0	38.0	34.0	38.0
74-75	36.6354609788133	38.0	38.0	38.0	34.0	38.0
76-77	36.612577669850026	38.0	38.0	38.0	34.0	38.0
78-79	36.63003840193724	38.0	38.0	38.0	34.0	38.0
80-81	36.65636485901739	38.0	38.0	38.0	34.0	38.0
82-83	36.524653783271894	38.0	38.0	38.0	34.0	38.0
84-85	36.5403610502248	38.0	38.0	38.0	34.0	38.0
86-87	36.53916768999082	38.0	38.0	38.0	34.0	38.0
88-89	36.487470736793696	38.0	38.0	38.0	34.0	38.0
90-91	36.487328173071944	38.0	38.0	38.0	34.0	38.0
92-93	36.535179893363264	38.0	38.0	38.0	34.0	38.0
94-95	36.43509334303464	38.0	38.0	38.0	34.0	38.0
96-97	36.394325659645766	38.0	38.0	38.0	34.0	38.0
98-99	36.46562301545873	38.0	38.0	38.0	34.0	38.0
100	34.83802624355249	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	17.0
20	41.0
21	210.0
22	807.0
23	2247.0
24	5401.0
25	10689.0
26	18045.0
27	28116.0
28	41272.0
29	58143.0
30	78088.0
31	101336.0
32	131411.0
33	175039.0
34	251483.0
35	421241.0
36	1052241.0
37	4215217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.669425358368706	19.838972472810465	15.231570947680998	26.26003122113983
2	13.081730413999477	20.912656213800325	43.65564378466729	22.349969587532907
3	16.15474749307659	27.655044790304178	31.607224163323366	24.582983553295858
4	11.450441788686987	19.838790407654507	39.93966360731459	28.77110419634392
5	12.936622718643639	37.68169222141841	36.54509547831407	12.836589581623883
6	27.885315927092602	39.55980583355055	19.842783679555566	12.712094559801281
7	24.78273937357752	33.40915335606012	24.756521991119165	17.05158527924319
8	23.306752326299602	37.23846909299843	22.36191002734467	17.092868553357306
9	24.81772999308456	17.09607245951553	22.274962122855765	35.811235424544144
10-11	22.943433721531648	27.44894703375655	30.126905482543215	19.480713762168588
12-13	23.998296477024063	25.003280965831376	30.029257870562905	20.969164686581664
14-15	21.358002757376788	26.422145070426595	27.42134140448399	24.798510767712624
16-17	21.036384659372022	29.41995406799557	28.198956857688923	21.344704414943482
18-19	21.015242343136077	28.618222567673996	29.677796259076892	20.688738830113028
20-21	21.74979985842729	27.75148292141683	29.452669220619548	21.046047999536334
22-23	21.495240390673583	27.849759043758425	29.327958806318488	21.3270417592495
24-25	21.37600043214769	28.003193158713596	29.385792643914627	21.235013765224085
26-27	21.29199781235944	28.100156485498385	29.38983402703715	21.21801167510503
28-29	21.056741647986225	27.926735626834937	29.469778965919254	21.546743759259588
30-31	21.338915882854543	27.744937017876765	29.530494828624388	21.38565227064431
32-33	21.197351577769787	27.933804496072046	29.49867760574688	21.370166320411286
34-35	21.295497236454285	28.167737592977954	29.501472951972403	21.035292218595362
36-37	21.35848080708402	28.023861027809748	29.20950822983181	21.408149935274416
38-39	21.287869941901572	27.94785086078045	29.45974468810213	21.30453450921585
40-41	21.447279894567792	27.817470796546246	29.416166374585963	21.319082934300003
42-43	21.54625499764487	27.835379790097946	29.28962441203894	21.32874080021824
44-45	21.42181270025646	28.08194903701328	29.139674480999584	21.356563781730674
46-47	21.5972743628057	27.918488845704214	28.825231452077194	21.659005339412886
48-49	21.542634375470687	27.97427579391219	28.88328268744256	21.599807143174562
50-51	21.399782388878968	28.080299816953808	28.940799664303242	21.579118129863982
52-53	21.514889691629605	28.158234907027648	28.943039648190155	21.383835753152592
54-55	21.515979058984414	28.135329083036815	28.760052008819052	21.588639849159716
56-57	21.687479613472238	28.264275954933492	28.501149522852764	21.547094908741503
58-59	21.362274139337188	28.428261913383295	28.900421014124422	21.30904293315509
60-61	21.616581225215914	28.625962773020717	28.544778336534645	21.212677665228725
62-63	21.397690438663894	28.806617918949325	28.478405106512426	21.317286535874352
64-65	21.398259945965545	28.706930824113737	28.479674862492033	21.41513436742869
66-67	21.498589015594092	28.686418175295376	28.33381760471447	21.481175204396063
68-69	21.435192873858234	28.569923588569317	28.49741241203459	21.497471125537857
70-71	21.5159930968241	28.268879480050597	28.530296717029557	21.684830706095745
72-73	21.62963714996166	28.081868385472735	28.582970327760854	21.70552413680475
74-75	21.553395283501345	28.21795231472088	28.506575236122355	21.72207716565542
76-77	21.62388830663338	28.177042760549753	28.41245074018635	21.786618192630517
78-79	21.577890234589557	28.152810305985394	28.45893734083571	21.81036211858934
80-81	21.588123785243262	28.205586871537786	28.44960938236976	21.756679960849194
82-83	21.659736202022405	28.162209319174075	28.481587317898626	21.696467160904888
84-85	21.738104950436846	28.182695085695116	28.36426893361254	21.714931030255496
86-87	21.607109941702237	28.19095318469151	28.467894666459447	21.734042207146807
88-89	21.702133826416432	28.149304138691562	28.383592743059733	21.764969291832273
90-91	21.720332070206734	28.143982082955738	28.41712907108132	21.718556775756213
92-93	21.705682842964418	28.15668782027252	28.433450436635454	21.704178900127612
94-95	21.582578812937914	28.236843509127606	28.444455570090778	21.736122107843702
96-97	21.66781774855994	28.359939533468314	28.504977755160027	21.467264962811715
98-99	22.688200177817723	30.252847024387396	28.790348634050595	18.268604163744286
100	21.590649188324722	31.153010717455416	22.723132040179063	24.5332080540408
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	965.0
1	951.5
2	1469.5
3	2134.5
4	2731.5
5	3813.0
6	4627.0
7	5378.5
8	6264.5
9	6655.5
10	6710.5
11	6858.5
12	7605.5
13	9341.5
14	12527.5
15	16330.5
16	19443.5
17	21569.0
18	21940.0
19	20865.0
20	19972.5
21	19923.5
22	22171.5
23	27189.0
24	34741.5
25	45288.0
26	58736.0
27	73443.5
28	88904.5
29	106948.5
30	124941.0
31	142400.0
32	160849.0
33	178200.0
34	194508.5
35	209421.0
36	224958.5
37	239275.5
38	248891.5
39	255445.0
40	263480.0
41	270048.0
42	274050.0
43	282436.0
44	290159.0
45	290820.5
46	284286.5
47	274067.0
48	261855.0
49	250231.5
50	240728.0
51	228736.0
52	213603.0
53	196771.5
54	179738.0
55	162327.5
56	145020.0
57	128128.5
58	110300.5
59	92567.5
60	75642.5
61	60225.0
62	46926.0
63	35418.5
64	25675.0
65	18505.0
66	13399.0
67	9885.5
68	7337.5
69	5267.5
70	3676.5
71	2528.0
72	1713.5
73	1093.5
74	681.5
75	424.0
76	266.0
77	161.5
78	98.5
79	63.5
80	36.0
81	21.0
82	17.5
83	13.5
84	11.5
85	10.0
86	8.0
87	8.0
88	6.5
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.003504754252245508
6	1.5172096330067134E-5
7	0.0
8	0.0
9	1.5172096330067134E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.5173722814473697E-5
26-27	0.0
28-29	3.7945229400540505E-5
30-31	0.0
32-33	3.0407722710152142E-5
34-35	0.0
36-37	4.885213884968633E-4
38-39	5.363306144180534E-5
40-41	2.0005487659153275E-4
42-43	5.409644561622498E-5
44-45	7.147109895204724E-4
46-47	0.0
48-49	5.505697531764335E-5
50-51	0.0
52-53	8.747524649331617E-5
54-55	0.0021901672680301122
56-57	0.0011172777519535683
58-59	1.132061435356866E-4
60-61	3.337227579467325E-4
62-63	1.3931958445721212E-4
64-65	7.756397625288101E-4
66-67	0.0013721296191808085
68-69	3.103418398590813E-4
70-71	6.179838201677242E-4
72-73	1.7946214681849505E-4
74-75	1.2051765777375477E-4
76-77	8.67383784884658E-6
78-79	0.0016787216953969978
80-81	0.0
82-83	0.001004490963537067
84-85	1.7922566626245304E-4
86-87	0.0
88-89	8.203056641194656E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.949856717846681E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	51.0
20-21	189.0
22-23	371.0
24-25	519.0
26-27	1014.0
28-29	2384.0
30-31	7138.0
32-33	9543.0
34-35	14243.0
36-37	23141.0
38-39	27329.0
40-41	27768.0
42-43	31892.0
44-45	38877.0
46-47	40034.0
48-49	37722.0
50-51	33519.0
52-53	31764.0
54-55	34178.0
56-57	36561.0
58-59	39008.0
60-61	42398.0
62-63	41219.0
64-65	45758.0
66-67	50090.0
68-69	51958.0
70-71	60592.0
72-73	42998.0
74-75	43240.0
76-77	45109.0
78-79	47142.0
80-81	47218.0
82-83	45174.0
84-85	45562.0
86-87	47370.0
88-89	49700.0
90-91	49969.0
92-93	50984.0
94-95	62406.0
96-97	206801.0
98-99	469744.0
100-101	4608370.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.44192217186706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85270026094429	76.77187422627702
2	7.085777371476955	12.108448774018216
3	1.7125033253691295	4.389587275357581
4	0.6292444729968381	2.1505542915549336
5	0.2870713859781266	1.2263965509256554
6	0.1511054522700606	0.7746444175561975
7	0.08564207104990659	0.512219621849854
8	0.0532761076022769	0.3641610431499007
9	0.03713721313007333	0.28557673865457883
>10	0.10422278562065444	1.301900651537958
>50	0.001065045479995994	0.05869525294414511
>100	2.2691951889780478E-4	0.043636468631766526
>500	2.7588562800659674E-5	0.012304687542080632
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5172096330067136E-5	0.0	0.0	0.0
3	0.0	1.5172096330067136E-5	0.0	0.0	0.0
4	0.0	1.5172096330067136E-5	0.0	0.0	0.0
5	0.0	1.5172096330067136E-5	0.0	0.0	0.0
6	1.0620467431046994E-4	1.5172096330067136E-5	0.0	0.0	0.0
7	1.0620467431046994E-4	1.5172096330067136E-5	0.0	0.0	0.0
8	1.0620467431046994E-4	1.5172096330067136E-5	0.0	0.0	0.0
9	1.0620467431046994E-4	4.551628899020141E-5	0.0	3.034419266013427E-5	0.0
10-11	1.1379072247550352E-4	4.551628899020141E-5	0.0	6.068838532026854E-5	0.0
12-13	1.2137677064053709E-4	4.551628899020141E-5	0.0	6.82744334853021E-5	0.0
14-15	1.2137677064053709E-4	1.2896281880557064E-4	0.0	7.586048165033567E-5	1.5172096330067136E-5
16-17	1.5172096330067134E-4	1.365488669706042E-4	0.0	7.586048165033567E-5	3.034419266013427E-5
18-19	1.5172096330067134E-4	1.896512041258392E-4	0.0	1.0620467431046994E-4	3.034419266013427E-5
20-21	1.5172096330067134E-4	2.730977339412084E-4	0.0	1.0620467431046994E-4	3.034419266013427E-5
22-23	1.5172096330067134E-4	2.8068378210624196E-4	0.0	1.2137677064053707E-4	3.034419266013427E-5
24-25	1.5930701146570493E-4	3.0344192660134274E-4	0.0	1.365488669706042E-4	3.034419266013427E-5
26-27	1.668930596307385E-4	3.1861402293140986E-4	0.0	1.5172096330067134E-4	3.034419266013427E-5
28-29	1.668930596307385E-4	3.1861402293140986E-4	0.0	2.80683782106242E-4	3.034419266013427E-5
30-31	1.668930596307385E-4	3.5654426375657765E-4	0.0	8.572234426487931E-4	3.034419266013427E-5
32-33	1.668930596307385E-4	3.6413031192161126E-4	0.0	0.002419949364645708	3.034419266013427E-5
34-35	1.668930596307385E-4	3.9447450458174555E-4	0.0	0.004475768417369805	3.034419266013427E-5
36-37	1.668930596307385E-4	4.248186972418798E-4	0.0	0.008602578619148066	3.034419266013427E-5
38-39	1.7447910779577205E-4	4.399907935719469E-4	0.0	0.02107404180246325	3.034419266013427E-5
40-41	1.8206515596080563E-4	4.8550708256214835E-4	0.0	0.03441031447659226	3.034419266013427E-5
42-43	1.9723725229087275E-4	4.930931307271819E-4	0.0	0.04257290230216838	3.793024082516784E-5
44-45	1.9723725229087275E-4	5.08265227057249E-4	0.0	0.05246510910937215	4.551628899020141E-5
46-47	1.9723725229087275E-4	5.158512752222826E-4	0.0	0.063502809189496	4.551628899020141E-5
48-49	1.9723725229087275E-4	5.234373233873161E-4	0.0	0.07384259283843675	4.551628899020141E-5
50-51	2.4275354128107417E-4	6.068838532026854E-4	0.0	0.08493339525571583	4.551628899020141E-5
52-53	2.4275354128107417E-4	6.372280458628197E-4	0.0	0.11500449018190889	6.068838532026854E-5
54-55	2.4275354128107417E-4	6.751582866879875E-4	0.0	0.15106097711031344	7.586048165033567E-5
56-57	2.4275354128107417E-4	6.979164311830882E-4	0.0	0.19255666057304704	8.344652981536924E-5
58-59	2.4275354128107417E-4	7.130885275131553E-4	0.0	0.2301075989899632	9.103257798040281E-5
60-61	2.9585587843630913E-4	7.282606238432224E-4	0.0	0.2436714531090432	9.103257798040281E-5
62-63	3.33786119261477E-4	7.586048165033568E-4	0.0	0.26004214504918566	9.103257798040281E-5
64-65	3.33786119261477E-4	8.572234426487931E-4	0.0	0.2665358022784544	9.103257798040281E-5
66-67	3.33786119261477E-4	9.027397316389946E-4	0.0	0.2718536220421429	9.103257798040281E-5
68-69	3.33786119261477E-4	9.406699724641624E-4	0.0	0.2771410976131713	9.103257798040281E-5
70-71	3.33786119261477E-4	9.634281169592631E-4	0.0	0.2817003125603565	9.103257798040281E-5
72-73	3.793024082516784E-4	9.710141651242967E-4	0.0	0.28499265746398106	9.103257798040281E-5
74-75	3.793024082516784E-4	0.001016530454114498	0.0	0.2875719138400925	9.103257798040281E-5
76-77	3.793024082516784E-4	0.0010544606949396658	0.0	0.2903256493239996	9.103257798040281E-5
78-79	3.793024082516784E-4	0.0010620467431046994	0.0	0.2920780264501224	9.103257798040281E-5
80-81	3.793024082516784E-4	0.0010620467431046994	0.0	0.29262422191800486	9.103257798040281E-5
82-83	3.793024082516784E-4	0.0010620467431046994	0.0	0.2930945569042369	9.103257798040281E-5
84-85	3.793024082516784E-4	0.0010923909357648336	0.0	0.29328420810836275	9.103257798040281E-5
86-87	3.793024082516784E-4	0.0010923909357648336	0.0	0.29335248254184804	9.103257798040281E-5
88	3.793024082516784E-4	0.0011530793210851023	0.0	0.29335248254184804	9.103257798040281E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6855	0.0	47.436768	1
GTATCAA	12595	0.0	37.963722	1
ATCAACG	14670	0.0	31.878899	3
TCAACGC	14830	0.0	31.5939	4
CAACGCA	15060	0.0	31.053347	5
AACGCAG	15705	0.0	29.83298	6
CTTATAC	7400	0.0	29.354475	1
TATCAAC	16165	0.0	29.065807	2
ACGCAGA	17430	0.0	26.805264	7
CGCAGAG	17905	0.0	26.069736	8
TATACAC	10535	0.0	23.440321	3
TTATACA	10615	0.0	21.534328	2
GCAGAGT	21770	0.0	21.521677	9
GTACATG	16205	0.0	19.850819	1
AGAGTAC	19655	0.0	19.657078	10-11
TACATGG	16095	0.0	19.443375	2
GAGTACT	12615	0.0	19.436378	12-13
ACACATC	11465	0.0	18.717472	6
ACATGGG	16215	0.0	18.544756	3
CAGAGTA	21245	0.0	18.473932	10-11
>>END_MODULE