FastQCFastQC Report
Fri 27 May 2016
SRR2138973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7933794
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87100.049.7681271
GTATCAA162600.037.2158621
ATCAACG188950.031.564793
TCAACGC190500.031.422314
CAACGCA194500.030.7312985
AACGCAG200650.029.7880556
TATCAAC206550.029.318112
CTTATAC57250.027.0146431
ACGCAGA225700.026.4433357
CGCAGAG227600.026.2417288
GCAGAGT272200.021.9100079
GTACATG195700.020.3693261
TACATGG192650.020.3299852
TATACAC92200.020.081883
GAGTACT167350.020.03134512-13
AGAGTAC248450.019.9627910-11
ACATGGG194600.019.588973
CAGAGTA271150.018.92617610-11
TATGCCG45450.018.26406542-43
TTATACA98950.017.699322
Produced by FastQC (version 0.11.5)