##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7933794
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.327500562782447	32.0	28.0	33.0	18.0	34.0
2	31.491564565452546	33.0	31.0	33.0	27.0	34.0
3	32.325304765916535	33.0	33.0	34.0	30.0	34.0
4	32.604059923915344	33.0	33.0	34.0	31.0	34.0
5	32.95452226261484	33.0	33.0	34.0	32.0	34.0
6	36.544960582540966	38.0	37.0	38.0	34.0	38.0
7	36.66727847987987	38.0	37.0	38.0	34.0	38.0
8	36.72051669100559	38.0	38.0	38.0	34.0	38.0
9	36.94277958313513	38.0	38.0	38.0	35.0	38.0
10-11	37.072229062161185	38.0	38.0	38.0	36.0	38.0
12-13	37.132038908496995	38.0	38.0	38.0	36.0	38.0
14-15	37.135119969084144	38.0	38.0	38.0	36.0	38.0
16-17	37.100322304814064	38.0	38.0	38.0	36.0	38.0
18-19	37.13759312379424	38.0	38.0	38.0	36.0	38.0
20-21	37.10675436084164	38.0	38.0	38.0	36.0	38.0
22-23	37.14900323562289	38.0	38.0	38.0	36.0	38.0
24-25	37.15453826416072	38.0	38.0	38.0	36.0	38.0
26-27	37.08784250070066	38.0	38.0	38.0	36.0	38.0
28-29	37.076355291284294	38.0	38.0	38.0	36.0	38.0
30-31	36.94722391617681	38.0	38.0	38.0	36.0	38.0
32-33	37.04163364570523	38.0	38.0	38.0	36.0	38.0
34-35	37.05474244170853	38.0	38.0	38.0	36.0	38.0
36-37	36.98150746775951	38.0	38.0	38.0	36.0	38.0
38-39	37.00749425967346	38.0	38.0	38.0	36.0	38.0
40-41	36.93597221972807	38.0	38.0	38.0	36.0	38.0
42-43	36.92038928412706	38.0	38.0	38.0	36.0	38.0
44-45	36.91642236660232	38.0	38.0	38.0	36.0	38.0
46-47	36.874687685192924	38.0	38.0	38.0	35.0	38.0
48-49	36.84119557985188	38.0	38.0	38.0	35.0	38.0
50-51	36.859623523518565	38.0	38.0	38.0	35.0	38.0
52-53	36.77494521870003	38.0	38.0	38.0	35.0	38.0
54-55	36.760735391896354	38.0	38.0	38.0	35.0	38.0
56-57	36.765842022183705	38.0	38.0	38.0	35.0	38.0
58-59	36.78680442281299	38.0	38.0	38.0	35.0	38.0
60-61	36.78266688666599	38.0	38.0	38.0	35.0	38.0
62-63	36.78779167647701	38.0	38.0	38.0	35.0	38.0
64-65	36.74391221562776	38.0	38.0	38.0	35.0	38.0
66-67	36.74954417567772	38.0	38.0	38.0	35.0	38.0
68-69	36.76268238259422	38.0	38.0	38.0	35.0	38.0
70-71	36.67882389944138	38.0	38.0	38.0	34.5	38.0
72-73	36.636279436089936	38.0	38.0	38.0	34.0	38.0
74-75	36.687020919465134	38.0	38.0	38.0	34.5	38.0
76-77	36.66654704790052	38.0	38.0	38.0	34.0	38.0
78-79	36.68431741328465	38.0	38.0	38.0	34.0	38.0
80-81	36.70974819508342	38.0	38.0	38.0	34.5	38.0
82-83	36.58476232204778	38.0	38.0	38.0	34.0	38.0
84-85	36.603219573708714	38.0	38.0	38.0	34.0	38.0
86-87	36.60164384494084	38.0	38.0	38.0	34.0	38.0
88-89	36.54816853500512	38.0	38.0	38.0	34.0	38.0
90-91	36.54825666453159	38.0	38.0	38.0	34.0	38.0
92-93	36.59704012603319	38.0	38.0	38.0	34.0	38.0
94-95	36.50000807836206	38.0	38.0	38.0	34.0	38.0
96-97	36.46317510381352	38.0	38.0	38.0	34.0	38.0
98-99	36.52961075526525	38.0	38.0	38.0	34.0	38.0
100	34.949270354232155	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	14.0
20	55.0
21	232.0
22	907.0
23	2542.0
24	6000.0
25	12085.0
26	20707.0
27	33186.0
28	48961.0
29	67959.0
30	91985.0
31	119602.0
32	156324.0
33	207518.0
34	297187.0
35	499530.0
36	1261400.0
37	5107594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.68787114966686	19.772860248199034	15.26978139336615	26.269487208767963
2	13.024890739537728	20.91805005272383	43.76287057617074	22.294188631567696
3	16.092275650212244	27.646243398807684	31.695869592782472	24.565611358197604
4	11.401228718567687	19.808744719109168	40.24113809861965	28.54888846370349
5	12.851382994559884	37.74930601011688	36.62539722514969	12.773913770173543
6	27.81665717772067	39.69526303496953	19.939290072226488	12.548789715083315
7	24.686184188800468	33.52117536704382	24.925766915551375	16.866873528604348
8	23.198169753336174	37.3753087110656	22.38108274553133	17.045438790066896
9	24.84913271002499	17.0615597027097	22.459594993265515	35.6297125939998
10-11	22.922797088001023	27.489836514535167	30.264127099846554	19.323239297617256
12-13	23.95208395882222	25.084707266157906	30.228148096610525	20.735060678409344
14-15	21.279050098855603	26.470563768103883	27.702187629272956	24.548198503767555
16-17	20.82151994367386	29.50249275441233	28.44460670393005	21.231380597983765
18-19	20.8200263329247	28.780826676366942	30.00215911832347	20.39698787238489
20-21	21.63889281553234	27.86962412138158	29.78332974000817	20.708153323077912
22-23	21.38343323776709	27.892717070769653	29.585714348738847	21.13813534272441
24-25	21.22609748446522	28.022448969172846	29.692520117578315	21.058933428783618
26-27	21.21477629200399	28.197151783876162	29.565247592941823	21.022824331178022
28-29	21.08256667530622	28.114872073960306	29.732074400373186	21.070486850360286
30-31	21.446554818103362	28.06148270850078	29.561691479112184	20.930270994283678
32-33	21.30632815264634	27.9705624018962	29.55423161367485	21.16887783178261
34-35	21.231683089896535	28.183453054222245	29.64179639091924	20.943067464961974
36-37	21.322492473433915	28.101495139524836	29.485332948035392	21.09067943900586
38-39	21.13711768047458	28.1172271537341	29.657938568816604	21.087716596974715
40-41	21.374720220147424	27.97679558958091	29.529094866045302	21.11938932422636
42-43	21.366546231678534	28.036731921740177	29.38628917376629	21.210432672815
44-45	21.324904602069438	28.18928489720296	29.303273511799716	21.18253698892789
46-47	21.446229258321075	28.070007151669568	29.019981948163693	21.463781641845664
48-49	21.38921882730034	28.177882921691072	29.024245313067226	21.408652937941365
50-51	21.312548081995136	28.27204850471498	28.976684751621306	21.438718661668577
52-53	21.44008867284655	28.275285053451537	28.966472036938896	21.318154236763018
54-55	21.405327982930505	28.194887130865133	28.914428656599828	21.485356229604534
56-57	21.51920912467606	28.28367796812841	28.78137331286419	21.415739594331342
58-59	21.342650438985245	28.496328368667857	28.939520384828874	21.221500807518023
60-61	21.54806885833146	28.55420039503133	28.72261270825623	21.175118038380976
62-63	21.384642880389094	28.699036730693177	28.707036170595646	21.20928421832208
64-65	21.390421894197942	28.617894059160022	28.663273831126666	21.328410215515365
66-67	21.427095838644153	28.62361331956856	28.601775777837556	21.34751506394973
68-69	21.41035249264163	28.501958385719934	28.69323622803033	21.39445289360811
70-71	21.47149040438954	28.271076886319857	28.745898706418892	21.511534002871706
72-73	21.492206572582777	28.178278643445203	28.801141998022388	21.528372785949625
74-75	21.445620840305757	28.285167729057086	28.712579338580372	21.55663209205678
76-77	21.542636030424013	28.23703685712867	28.63330833008595	21.58701878236137
78-79	21.478630608065647	28.265224415911256	28.6094038544803	21.6467411215428
80-81	21.506556123551395	28.272711397980206	28.639043234873647	21.58168924359476
82-83	21.536585669556576	28.25535035418015	28.69596688527516	21.512097090988114
84-85	21.65993010153571	28.252943423613868	28.571378396281013	21.515748078569413
86-87	21.50545680673566	28.28700789869987	28.607956634919944	21.59957865964453
88-89	21.600452629800735	28.262654529027348	28.546057530269465	21.590835310902452
90-91	21.624124044821468	28.31388991429529	28.55289507459387	21.509090966289367
92-93	21.644574705020155	28.27952830485312	28.544865731254333	21.531031258872396
94-95	21.5717589125481	28.351978825989004	28.522117072602253	21.554145188860648
96-97	21.61024510625759	28.47194703123968	28.57974780651618	21.338060055986546
98-99	22.636623736211288	30.35225079744578	28.88492146573555	18.12620400060738
100	21.459746740140037	31.34850344311588	22.87404167721886	24.31770813952522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	941.0
1	983.5
2	1740.0
3	2653.0
4	3378.0
5	4814.0
6	5996.5
7	7059.5
8	8377.5
9	9171.0
10	9241.0
11	9282.0
12	10187.5
13	12296.0
14	16414.0
15	21242.0
16	25076.5
17	27556.0
18	28018.0
19	26875.0
20	25756.5
21	25618.0
22	28128.0
23	34486.5
24	44508.0
25	58591.0
26	75753.5
27	94866.0
28	115407.5
29	137865.0
30	159736.0
31	180317.5
32	201646.5
33	221570.0
34	240100.5
35	258032.0
36	275405.5
37	290639.0
38	302214.0
39	310070.5
40	318361.5
41	326379.0
42	329782.0
43	335379.5
44	341788.5
45	340425.5
46	331536.5
47	321160.5
48	311686.5
49	299065.0
50	285108.5
51	270193.5
52	252659.5
53	232856.5
54	212102.0
55	191632.5
56	171898.0
57	153153.5
58	133666.5
59	112481.5
60	90912.5
61	71523.0
62	54668.0
63	40585.5
64	29303.5
65	20918.0
66	14784.0
67	10495.5
68	7507.0
69	5304.5
70	3746.0
71	2552.5
72	1705.5
73	1117.5
74	664.0
75	372.5
76	234.5
77	139.0
78	91.0
79	61.0
80	41.5
81	31.5
82	23.5
83	22.5
84	23.0
85	19.5
86	15.0
87	10.0
88	5.0
89	4.0
90	3.0
91	1.5
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.00404598354835026
6	1.2604310119471213E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.8908531491591944E-5
26-27	0.0
28-29	3.1523541591719446E-5
30-31	0.0
32-33	5.052621473704421E-5
34-35	0.0
36-37	5.200987730996796E-4
38-39	5.094205551321351E-5
40-41	1.854855272358953E-4
42-43	7.068027386164439E-5
44-45	7.107023438381816E-4
46-47	0.0
48-49	7.20023789586008E-5
50-51	1.975633719088988E-5
52-53	1.788183090079723E-4
54-55	0.002271245301819194
56-57	0.0011662556397508718
58-59	2.226514221960798E-4
60-61	5.025991507568566E-4
62-63	1.5041659242696215E-4
64-65	8.19197303670589E-4
66-67	0.0013388148436413406
68-69	3.9871250834585723E-4
70-71	7.90281513092742E-4
72-73	2.349374482603665E-4
74-75	2.0800669408577434E-4
76-77	0.0
78-79	0.0017417831924480944
80-81	0.0
82-83	9.783476834209202E-4
84-85	1.6440340470483687E-4
86-87	2.2607325451666104E-5
88-89	1.1403922447549369E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.0958889690380146E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	59.0
20-21	217.0
22-23	459.0
24-25	627.0
26-27	1266.0
28-29	2972.0
30-31	8867.0
32-33	11923.0
34-35	17696.0
36-37	28868.0
38-39	34882.0
40-41	35025.0
42-43	40338.0
44-45	49226.0
46-47	50816.0
48-49	47106.0
50-51	43521.0
52-53	41894.0
54-55	45910.0
56-57	49253.0
58-59	48744.0
60-61	49156.0
62-63	48965.0
64-65	53823.0
66-67	58669.0
68-69	60453.0
70-71	66607.0
72-73	52528.0
74-75	52896.0
76-77	56029.0
78-79	57190.0
80-81	57779.0
82-83	55339.0
84-85	55474.0
86-87	57697.0
88-89	59925.0
90-91	60407.0
92-93	61442.0
94-95	74630.0
96-97	245276.0
98-99	553866.0
100-101	5535974.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.27766701654066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7585978301137	76.54403817628025
2	7.110012672097119	12.126505862689651
3	1.7778754632488156	4.548392152554315
4	0.6297055778885501	2.147992903585523
5	0.29120369808858787	1.2416585999791916
6	0.15535878517231172	0.7949180850011204
7	0.08065019454152296	0.4814362304451854
8	0.05535620171957719	0.3776518190034047
9	0.03653020182652376	0.2803689348668386
>10	0.10314766710326943	1.3108734348111302
>50	0.0011982444666296158	0.06887524085033064
>100	2.941991623161458E-4	0.04052910894043677
>500	6.926457108751172E-5	0.03675945099260379
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2604310119471213E-5	0.0
2	0.0	2.5208620238942427E-5	0.0	1.2604310119471213E-5	0.0
3	0.0	2.5208620238942427E-5	0.0	1.2604310119471213E-5	0.0
4	0.0	8.823017083629849E-5	0.0	3.7812930358413644E-5	0.0
5	0.0	8.823017083629849E-5	0.0	3.7812930358413644E-5	0.0
6	1.2604310119471213E-5	8.823017083629849E-5	0.0	3.7812930358413644E-5	0.0
7	1.2604310119471213E-5	8.823017083629849E-5	0.0	5.0417240477884854E-5	0.0
8	1.2604310119471213E-5	8.823017083629849E-5	0.0	7.562586071682729E-5	0.0
9	1.2604310119471213E-5	1.2604310119471214E-4	0.0	1.2604310119471214E-4	0.0
10-11	1.2604310119471213E-5	1.2604310119471214E-4	0.0	1.3864741131418336E-4	0.0
12-13	1.2604310119471213E-5	1.2604310119471214E-4	0.0	2.0166896191153942E-4	0.0
14-15	2.5208620238942427E-5	2.3317973721021746E-4	0.0	2.0166896191153942E-4	0.0
16-17	5.0417240477884854E-5	2.3948189226995307E-4	0.0	2.0166896191153942E-4	0.0
18-19	5.0417240477884854E-5	2.520862023894243E-4	0.0	2.772948226283667E-4	0.0
20-21	5.0417240477884854E-5	2.646905125088955E-4	0.0	3.277120631062516E-4	0.0
22-23	5.0417240477884854E-5	3.1510775298678037E-4	0.0	3.781293035841364E-4	0.0
24-25	5.0417240477884854E-5	3.5292068334519396E-4	0.0	3.9703576876334325E-4	0.0
26-27	5.0417240477884854E-5	3.655249934646652E-4	0.0	4.3484869912175685E-4	0.0
28-29	5.0417240477884854E-5	3.718271485244008E-4	0.0	6.239133509138252E-4	0.0
30-31	5.0417240477884854E-5	4.2854654406202126E-4	0.0	0.0010650642050953176	0.0
32-33	5.0417240477884854E-5	4.411508541814925E-4	0.0	0.001909552983099889	0.0
34-35	5.0417240477884854E-5	5.29381025017791E-4	0.0	0.003100660289389919	0.0
36-37	5.0417240477884854E-5	5.92402575615147E-4	0.0	0.005199277924281875	0.0
38-39	5.0417240477884854E-5	6.365176610332963E-4	0.0	0.008552024416061219	0.0
40-41	5.0417240477884854E-5	7.310499869293304E-4	0.0	0.012169461420349456	0.0
42-43	5.0417240477884854E-5	7.310499869293304E-4	0.0	0.015641948858263775	0.0
44-45	5.0417240477884854E-5	7.940715375266865E-4	0.0	0.01972574533697245	0.0
46-47	5.0417240477884854E-5	8.444887780045714E-4	0.0	0.024118347413608167	0.0
48-49	5.0417240477884854E-5	8.444887780045714E-4	0.0	0.02880715077805146	0.0
50-51	5.0417240477884854E-5	9.327189488408698E-4	0.0	0.033666112329107614	0.0
52-53	5.0417240477884854E-5	9.45323258960341E-4	0.0	0.0389032031837479	0.0
54-55	6.302155059735607E-5	9.516254140200767E-4	0.0	0.04369284102914696	0.0
56-57	7.562586071682729E-5	9.579275690798123E-4	0.0	0.04879758662753281	0.0
58-59	7.562586071682729E-5	9.642297241395478E-4	0.0	0.05410400118783018	0.0
60-61	8.192801577656288E-5	9.894383443784902E-4	0.0	0.05910791230526026	0.0
62-63	8.823017083629849E-5	0.0010146469646174327	0.0	0.06378411135958408	0.0
64-65	8.823017083629849E-5	0.0010965749803939957	0.0	0.06886995049279071	0.0
66-67	9.45323258960341E-5	0.0010965749803939957	0.0	0.07403141548671417	0.0
68-69	1.0083448095576971E-4	0.0011469922208718806	0.0	0.07894709643330794	0.0
70-71	1.0713663601550532E-4	0.0011658986860510873	0.0	0.08337751144030209	0.0
72-73	1.1974094613497653E-4	0.001172200841110823	0.0	0.08673656008714116	0.0
74-75	1.2604310119471214E-4	0.001216315926528972	0.0	0.08968596865509743	0.0
76-77	1.2604310119471214E-4	0.0012415245467679145	0.0	0.09214380912839432	0.0
78-79	1.2604310119471214E-4	0.00124782670182765	0.0	0.09371304573826847	0.0
80-81	1.2604310119471214E-4	0.00124782670182765	0.0	0.09422352029810707	0.0
82-83	1.2604310119471214E-4	0.0012604310119471215	0.0	0.09459534744663146	0.0
84-85	1.3864741131418336E-4	0.0012604310119471215	0.0	0.09482852718384169	0.0
86-87	1.4494956637391897E-4	0.001329754717604213	0.0	0.09502389399069348	0.0
88	1.5125172143365458E-4	0.0014368913536197184	0.0	0.09504910261093243	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8710	0.0	49.768127	1
GTATCAA	16260	0.0	37.215862	1
ATCAACG	18895	0.0	31.56479	3
TCAACGC	19050	0.0	31.42231	4
CAACGCA	19450	0.0	30.731298	5
AACGCAG	20065	0.0	29.788055	6
TATCAAC	20655	0.0	29.31811	2
CTTATAC	5725	0.0	27.014643	1
ACGCAGA	22570	0.0	26.443335	7
CGCAGAG	22760	0.0	26.241728	8
GCAGAGT	27220	0.0	21.910007	9
GTACATG	19570	0.0	20.369326	1
TACATGG	19265	0.0	20.329985	2
TATACAC	9220	0.0	20.08188	3
GAGTACT	16735	0.0	20.031345	12-13
AGAGTAC	24845	0.0	19.96279	10-11
ACATGGG	19460	0.0	19.58897	3
CAGAGTA	27115	0.0	18.926176	10-11
TATGCCG	4545	0.0	18.264065	42-43
TTATACA	9895	0.0	17.69932	2
>>END_MODULE