FastQCFastQC Report
Fri 27 May 2016
SRR2138972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6027683
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63000.048.1215481
GTATCAA119700.038.776461
ATCAACG136150.033.320233
TCAACGC136500.033.2347954
CAACGCA140150.032.4628875
AACGCAG145950.031.2604086
TATCAAC150650.030.5487542
ACGCAGA164950.027.4747
CGCAGAG166750.027.1774278
GCAGAGT206400.021.9565729
AGAGTAC183800.020.0630310-11
GAGTACT123200.019.67041612-13
GTACATG144400.019.5103721
TACATGG144700.019.4094562
ACATGGG143700.018.8747773
CAGAGTA201850.018.5398310-11
CTTATAC39700.017.8513641
TATGCCG20600.017.26743142-43
AGTACTT138300.017.23807512-13
CATGGGG100000.016.886294
Produced by FastQC (version 0.11.5)