##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6027683
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.433014974410565	32.0	28.0	33.0	18.0	34.0
2	31.554219589849033	33.0	31.0	33.0	27.0	34.0
3	32.34898600341126	33.0	33.0	34.0	30.0	34.0
4	32.61915366153131	33.0	33.0	34.0	31.0	34.0
5	32.965988091941796	33.0	33.0	34.0	32.0	34.0
6	36.52905320336189	38.0	37.0	38.0	34.0	38.0
7	36.65061964937439	38.0	37.0	38.0	34.0	38.0
8	36.702171796360226	38.0	38.0	38.0	34.0	38.0
9	36.93915522763888	38.0	38.0	38.0	35.0	38.0
10-11	37.07049308996508	38.0	38.0	38.0	36.0	38.0
12-13	37.13106512071056	38.0	38.0	38.0	36.0	38.0
14-15	37.13258792806457	38.0	38.0	38.0	36.0	38.0
16-17	37.09328808432693	38.0	38.0	38.0	36.0	38.0
18-19	37.13492995567285	38.0	38.0	38.0	36.0	38.0
20-21	37.1048289443387	38.0	38.0	38.0	36.0	38.0
22-23	37.14660532370566	38.0	38.0	38.0	36.0	38.0
24-25	37.1535933276257	38.0	38.0	38.0	36.0	38.0
26-27	37.08793531766905	38.0	38.0	38.0	36.0	38.0
28-29	37.07925614143297	38.0	38.0	38.0	36.0	38.0
30-31	36.94729726886822	38.0	38.0	38.0	36.0	38.0
32-33	37.04440020947522	38.0	38.0	38.0	36.0	38.0
34-35	37.06031852450961	38.0	38.0	38.0	36.0	38.0
36-37	36.98660023132064	38.0	38.0	38.0	36.0	38.0
38-39	37.021129718891615	38.0	38.0	38.0	36.0	38.0
40-41	36.938820493628874	38.0	38.0	38.0	36.0	38.0
42-43	36.919934582923275	38.0	38.0	38.0	36.0	38.0
44-45	36.91801520377327	38.0	38.0	38.0	36.0	38.0
46-47	36.87692207780178	38.0	38.0	38.0	35.0	38.0
48-49	36.843192243274984	38.0	38.0	38.0	35.0	38.0
50-51	36.858789721174176	38.0	38.0	38.0	35.0	38.0
52-53	36.77019921890688	38.0	38.0	38.0	35.0	38.0
54-55	36.75840026027273	38.0	38.0	38.0	35.0	38.0
56-57	36.761595103934624	38.0	38.0	38.0	35.0	38.0
58-59	36.78026845320046	38.0	38.0	38.0	35.0	38.0
60-61	36.77646265535806	38.0	38.0	38.0	35.0	38.0
62-63	36.779921632950504	38.0	38.0	38.0	35.0	38.0
64-65	36.737721234563736	38.0	38.0	38.0	35.0	38.0
66-67	36.740419924208425	38.0	38.0	38.0	35.0	38.0
68-69	36.75151779494006	38.0	38.0	38.0	35.0	38.0
70-71	36.66724542537872	38.0	38.0	38.0	34.0	38.0
72-73	36.625266288629064	38.0	38.0	38.0	34.0	38.0
74-75	36.67143715393969	38.0	38.0	38.0	34.0	38.0
76-77	36.64910781579312	38.0	38.0	38.0	34.0	38.0
78-79	36.666510243214205	38.0	38.0	38.0	34.0	38.0
80-81	36.688907123912145	38.0	38.0	38.0	34.0	38.0
82-83	36.56023364985389	38.0	38.0	38.0	34.0	38.0
84-85	36.57695728207131	38.0	38.0	38.0	34.0	38.0
86-87	36.57150101083822	38.0	38.0	38.0	34.0	38.0
88-89	36.52033577185473	38.0	38.0	38.0	34.0	38.0
90-91	36.5173570262035	38.0	38.0	38.0	34.0	38.0
92-93	36.56454260479263	38.0	38.0	38.0	34.0	38.0
94-95	36.46806932688968	38.0	38.0	38.0	34.0	38.0
96-97	36.427756708288456	38.0	38.0	38.0	34.0	38.0
98-99	36.49415551441366	38.0	38.0	38.0	34.0	38.0
100	34.88974020070599	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	11.0
20	39.0
21	208.0
22	605.0
23	1955.0
24	4398.0
25	8941.0
26	15725.0
27	24660.0
28	36791.0
29	51774.0
30	69904.0
31	91762.0
32	119872.0
33	160180.0
34	228924.0
35	383483.0
36	959229.0
37	3869220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.035546494399256	19.566589682967734	15.211831810000625	26.18603201263238
2	13.094749674128517	20.97748006987096	43.564782686813494	22.36298756918703
3	16.306663771137266	27.400545118248587	31.55131084365253	24.741480266961617
4	11.444447227898348	19.708767033701008	40.25719335273604	28.58959238566461
5	12.949585621953632	37.63745201757223	36.556135437987464	12.856826922486677
6	28.01179159554343	39.60958132668888	19.88362692596807	12.495000151799623
7	24.89621634050762	33.463720636934625	24.7530767626632	16.886986259894556
8	23.31114625636418	37.29680210455659	22.325527072342723	17.066524566736504
9	24.992886984932685	17.044791506122667	22.2810987240039	35.68122278494074
10-11	22.936474927430655	27.44489051597438	30.25833475317133	19.360299803423636
12-13	24.00705212931735	25.087757601055	30.13849600252701	20.76669426710064
14-15	21.35766761457097	26.495636880705238	27.654846812614398	24.49184869210939
16-17	20.915441306385887	29.406199695637607	28.40734159377658	21.27101740419992
18-19	20.88866484849983	28.719152948156037	29.928282558986595	20.463899644357543
20-21	21.721210403866397	27.942110527953425	29.67953627502841	20.657142793151763
22-23	21.447117594065794	27.88424751511009	29.469151860743303	21.199483030080817
24-25	21.295057981213628	27.944437121017856	29.572007612639716	21.188497285128797
26-27	21.265010995943694	28.15091012829002	29.46305596054652	21.12102291521977
28-29	21.154673593674808	28.129541004940084	29.549195397510612	21.166590003874493
30-31	21.454955196936947	27.997204567179935	29.502377587516897	21.045462648366218
32-33	21.225241835531058	28.078690792169823	29.457342431796853	21.238724940502266
34-35	21.29874805370796	28.079758154818986	29.441989819209752	21.1795039722633
36-37	21.29976211343433	28.06321939818872	29.471524560744545	21.165493927632404
38-39	21.1773229666493	28.15942593284781	29.547429104421024	21.11582199608187
40-41	21.41481476869037	28.036200258597315	29.407711841202467	21.14127313150985
42-43	21.37243880294201	28.072694856092756	29.261068148237168	21.293798192728065
44-45	21.413406240168253	28.172898902259806	29.134112954333453	21.279581903238487
46-47	21.467992934763185	28.14032043232765	28.92986697360899	21.461819659300176
48-49	21.441713243809197	28.211280268474326	28.951653128255376	21.395353359461105
50-51	21.39538965628323	28.278059765073994	28.908190733233706	21.418359845409068
52-53	21.53914330436859	28.25143191928095	28.854041617952603	21.35538315839785
54-55	21.509003953283813	28.193906851346362	28.811355296449026	21.4857338989208
56-57	21.56020560330284	28.28474486608102	28.722747242761653	21.432302287854483
58-59	21.45697708196235	28.347125439785675	28.8579334756764	21.337964002575575
60-61	21.58439116000201	28.401164369688182	28.756670477794895	21.25777399251492
62-63	21.48699014887314	28.45119965103019	28.71517987248421	21.34663032761246
64-65	21.479361967074258	28.417948352881744	28.664188575364307	21.438501104679695
66-67	21.539306734477197	28.421173212837893	28.612463551884183	21.427056500800727
68-69	21.505981682378483	28.38156751169733	28.668862839907078	21.44358796601711
70-71	21.542697692850613	28.173472925099226	28.70260264653582	21.581226735514342
72-73	21.55381651728243	28.14409260021723	28.740319212160394	21.561771670339947
74-75	21.520524956990926	28.22107343702426	28.64784729445135	21.61055431153347
76-77	21.582178965137526	28.2038255242682	28.553766230230277	21.660229280363993
78-79	21.583927893635067	28.239502729709105	28.544659897276524	21.631909479379306
80-81	21.561264465172904	28.252358084509815	28.561683739977372	21.62469371033991
82-83	21.62510105096882	28.242275909003467	28.546489451082806	21.586133588944904
84-85	21.73522718064027	28.205694234394844	28.517489741063766	21.54158884390112
86-87	21.558830972743387	28.222739524602197	28.549894070476427	21.668535432177986
88-89	21.664713507022242	28.24174530268746	28.467030453405723	21.626510736884576
90-91	21.71115736585679	28.25449548047952	28.49384375601	21.540503397653694
92-93	21.66312947964236	28.259499818373413	28.503811328283636	21.57355937370059
94-95	21.59885283369446	28.310259421675767	28.466513073028278	21.6243746716015
96-97	21.634358160374763	28.43631772182085	28.56696274499307	21.362361372811318
98-99	22.676459033031367	30.306323397798057	28.854543736762178	18.162673832408395
100	21.552293237089017	31.272062145107732	22.886089307657702	24.289555310145545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	668.0
1	695.5
2	1202.5
3	1865.0
4	2482.0
5	3550.0
6	4387.0
7	5142.5
8	5967.5
9	6392.5
10	6497.0
11	6626.0
12	7363.5
13	8945.0
14	11943.5
15	15529.5
16	18422.5
17	20226.5
18	20541.5
19	19856.0
20	19122.0
21	19090.0
22	20999.5
23	25629.5
24	32480.5
25	41987.0
26	55007.5
27	69287.0
28	84131.0
29	100012.5
30	115669.0
31	131477.5
32	148049.0
33	163756.5
34	178227.0
35	192010.5
36	206023.5
37	218582.0
38	227413.5
39	234158.5
40	241538.0
41	248534.0
42	252443.0
43	254603.0
44	257047.5
45	260120.0
46	257346.5
47	251302.5
48	244987.0
49	235065.5
50	224196.5
51	211013.5
52	195546.5
53	179668.5
54	163802.0
55	146968.5
56	130309.5
57	113803.0
58	97584.5
59	82305.5
60	66857.5
61	52584.0
62	39843.0
63	29643.5
64	21765.5
65	15685.5
66	11208.0
67	8196.0
68	6058.0
69	4251.5
70	3040.0
71	2243.0
72	1581.5
73	1007.0
74	625.0
75	382.5
76	240.5
77	139.5
78	85.5
79	59.5
80	42.0
81	24.0
82	14.0
83	8.5
84	4.0
85	2.5
86	4.0
87	6.0
88	6.5
89	5.0
90	4.0
91	3.5
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0035834664828923485
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.6591775539823785E-5
26-27	0.0
28-29	3.319333215662971E-5
30-31	0.0
32-33	3.325055607398714E-5
34-35	0.0
36-37	5.675857772352696E-4
38-39	4.189437656810652E-5
40-41	2.1036441933235548E-4
42-43	5.9160015672333293E-5
44-45	6.711987592848453E-4
46-47	0.0
48-49	3.441712575527307E-5
50-51	8.654931099391756E-6
52-53	1.4794780297077448E-4
54-55	0.0023103243406583742
56-57	0.0012767275201962887
58-59	1.5064924062591745E-4
60-61	5.083856881401646E-4
62-63	4.487778432993429E-5
64-65	7.227161987440638E-4
66-67	0.0013465843297071698
68-69	4.309319664782607E-4
70-71	8.317546168619394E-4
72-73	1.7698786953087689E-4
74-75	2.1580726272132206E-4
76-77	0.0
78-79	0.0015247143328579085
80-81	0.0
82-83	8.814337032545922E-4
84-85	1.7574163948203862E-4
86-87	0.0
88-89	7.942357545875057E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.2780248571859622E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	28.0
20-21	155.0
22-23	331.0
24-25	462.0
26-27	979.0
28-29	2252.0
30-31	6559.0
32-33	8779.0
34-35	13017.0
36-37	21258.0
38-39	25694.0
40-41	25109.0
42-43	29517.0
44-45	36252.0
46-47	37390.0
48-49	34679.0
50-51	32186.0
52-53	31354.0
54-55	34099.0
56-57	36354.0
58-59	36208.0
60-61	35705.0
62-63	35416.0
64-65	38370.0
66-67	41660.0
68-69	42224.0
70-71	44331.0
72-73	38887.0
74-75	39362.0
76-77	41512.0
78-79	42676.0
80-81	42483.0
82-83	40930.0
84-85	41345.0
86-87	42958.0
88-89	44294.0
90-91	44728.0
92-93	45652.0
94-95	56119.0
96-97	188186.0
98-99	426755.0
100-101	4241428.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.67187845089806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48436937707277	81.12090881560857
2	6.358188128599131	11.275849698141705
3	1.3171516998730466	3.503829462976097
4	0.44033614801442106	1.5618173357708562
5	0.17670849411754952	0.7834537055816295
6	0.08977971065138211	0.4776561354141692
7	0.04833336888993159	0.3000067427931302
8	0.02426217077874785	0.1721097806598448
9	0.015945125487148923	0.12724958061727076
>10	0.04400961723372444	0.55799265894215
>50	5.279729445978421E-4	0.030378907062554292
>100	3.3768161358783066E-4	0.06413865224859697
>500	5.050472397494362E-5	0.024608524183364865
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6590122605983094E-5	0.0
2	0.0	1.6590122605983094E-5	0.0	1.6590122605983094E-5	0.0
3	0.0	1.6590122605983094E-5	0.0	1.6590122605983094E-5	0.0
4	0.0	1.6590122605983094E-5	0.0	1.6590122605983094E-5	0.0
5	0.0	3.318024521196619E-5	0.0	1.6590122605983094E-5	0.0
6	8.295061302991547E-5	3.318024521196619E-5	0.0	1.6590122605983094E-5	3.318024521196619E-5
7	8.295061302991547E-5	3.318024521196619E-5	0.0	1.6590122605983094E-5	3.318024521196619E-5
8	8.295061302991547E-5	3.318024521196619E-5	0.0	4.977036781794928E-5	3.318024521196619E-5
9	1.3272098084786475E-4	9.954073563589856E-5	0.0	1.1613085824188167E-4	4.977036781794928E-5
10-11	1.3272098084786475E-4	1.0783579693889012E-4	0.0	1.2442591954487322E-4	5.806542912094083E-5
12-13	1.3272098084786475E-4	1.1613085824188167E-4	0.0	1.410160421508563E-4	6.636049042393238E-5
14-15	1.3272098084786475E-4	1.6590122605983094E-4	0.0	1.4931110345384785E-4	1.0783579693889012E-4
16-17	1.6590122605983094E-4	1.9078640996880557E-4	0.0	1.6590122605983094E-4	1.1613085824188167E-4
18-19	1.6590122605983094E-4	2.073765325747887E-4	0.0	2.073765325747887E-4	1.1613085824188167E-4
20-21	1.6590122605983094E-4	2.1567159387778025E-4	0.0	2.4055677778675488E-4	1.1613085824188167E-4
22-23	1.6590122605983094E-4	2.3226171648376334E-4	0.0	2.820320843017126E-4	1.3272098084786475E-4
24-25	1.741962873628225E-4	2.7373702299872106E-4	0.0	3.152123295136788E-4	1.3272098084786475E-4
26-27	1.8249134866581403E-4	2.986222069076957E-4	0.0	3.152123295136788E-4	1.3272098084786475E-4
28-29	1.8249134866581403E-4	2.986222069076957E-4	0.0	4.2304812645256894E-4	1.3272098084786475E-4
30-31	1.9078640996880557E-4	2.986222069076957E-4	0.0	8.129160076931716E-4	1.3272098084786475E-4
32-33	1.9908147127179713E-4	3.318024521196619E-4	0.0	0.0016756023832042925	1.6590122605983094E-4
34-35	2.1567159387778025E-4	3.815728199376112E-4	0.0	0.002953041823864991	1.6590122605983094E-4
36-37	2.1567159387778025E-4	4.147530651495774E-4	0.0	0.0049853318430979204	1.741962873628225E-4
38-39	2.1567159387778025E-4	4.479333103615436E-4	0.0	0.008104274893022742	1.8249134866581403E-4
40-41	2.3226171648376334E-4	4.977036781794929E-4	0.0	0.01184534754067193	1.8249134866581403E-4
42-43	2.3226171648376334E-4	5.059987394824845E-4	0.0	0.01538733871704932	1.8249134866581403E-4
44-45	2.4055677778675488E-4	5.391789846944506E-4	0.0	0.019443623694212188	1.8249134866581403E-4
46-47	2.4885183908974644E-4	5.640641686034252E-4	0.0	0.02407226790128147	1.8249134866581403E-4
48-49	2.4885183908974644E-4	5.806542912094083E-4	0.0	0.028908288640925546	1.8249134866581403E-4
50-51	2.986222069076957E-4	6.304246590273576E-4	0.0	0.0336613587675397	1.8249134866581403E-4
52-53	2.986222069076957E-4	6.470147816333407E-4	0.0	0.03875452640757651	1.8249134866581403E-4
54-55	3.0691726821068725E-4	6.636049042393238E-4	0.0	0.04378962861849238	1.8249134866581403E-4
56-57	3.152123295136788E-4	6.9678514945129E-4	0.0	0.0484846333159856	1.8249134866581403E-4
58-59	3.152123295136788E-4	6.9678514945129E-4	0.0	0.05359439107862839	1.9078640996880557E-4
60-61	3.152123295136788E-4	7.382604559662477E-4	0.0	0.058364051327848526	1.9908147127179713E-4
62-63	3.4009751342265344E-4	7.631456398752224E-4	0.0	0.06314200663837166	1.9908147127179713E-4
64-65	3.48392574725645E-4	7.963258850871885E-4	0.0	0.06753009406765419	1.9908147127179713E-4
66-67	3.48392574725645E-4	8.295061302991547E-4	0.0	0.07239099999120724	1.9908147127179713E-4
68-69	3.48392574725645E-4	8.295061302991547E-4	0.0	0.07730167628257822	1.9908147127179713E-4
70-71	3.649826973316281E-4	8.460962529051379E-4	0.0	0.08159022297622487	2.073765325747887E-4
72-73	3.8986788124060275E-4	8.62686375511121E-4	0.0	0.08533129562387405	2.3226171648376334E-4
74-75	3.9816294254359425E-4	8.709814368141125E-4	0.0	0.08791935475040741	2.3226171648376334E-4
76-77	4.064580038465858E-4	9.207518046320618E-4	0.0	0.09039128301869889	2.3226171648376334E-4
78-79	4.147530651495774E-4	9.290468659350534E-4	0.0	0.09203370515669121	2.3226171648376334E-4
80-81	4.147530651495774E-4	9.290468659350534E-4	0.0	0.09259776932529465	2.4055677778675488E-4
82-83	4.147530651495774E-4	9.622271111470195E-4	0.0	0.09317842361650405	2.57146900392738E-4
84-85	4.313431877555605E-4	0.0010119974789649688	0.0	0.09346045570080577	2.654419616957295E-4
86-87	4.313431877555605E-4	0.0010119974789649688	0.0	0.0935351112525327	2.654419616957295E-4
88	4.313431877555605E-4	0.001061767846782918	0.0	0.0935351112525327	2.654419616957295E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6300	0.0	48.121548	1
GTATCAA	11970	0.0	38.77646	1
ATCAACG	13615	0.0	33.32023	3
TCAACGC	13650	0.0	33.234795	4
CAACGCA	14015	0.0	32.462887	5
AACGCAG	14595	0.0	31.260408	6
TATCAAC	15065	0.0	30.548754	2
ACGCAGA	16495	0.0	27.474	7
CGCAGAG	16675	0.0	27.177427	8
GCAGAGT	20640	0.0	21.956572	9
AGAGTAC	18380	0.0	20.06303	10-11
GAGTACT	12320	0.0	19.670416	12-13
GTACATG	14440	0.0	19.510372	1
TACATGG	14470	0.0	19.409456	2
ACATGGG	14370	0.0	18.874777	3
CAGAGTA	20185	0.0	18.53983	10-11
CTTATAC	3970	0.0	17.851364	1
TATGCCG	2060	0.0	17.267431	42-43
AGTACTT	13830	0.0	17.238075	12-13
CATGGGG	10000	0.0	16.88629	4
>>END_MODULE