FastQCFastQC Report
Fri 27 May 2016
SRR2138971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6261503
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.045.6030041
GTATCAA118800.035.964771
ATCAACG136150.030.6084083
TCAACGC137100.030.52434
CAACGCA139800.029.9033975
AACGCAG145050.028.909716
TATCAAC150750.028.1095942
ACGCAGA162400.025.7941347
CGCAGAG164350.025.4880858
CTTATAC42900.020.3484151
GCAGAGT207200.020.1535159
GTACATG145650.019.3356761
AGAGTAC182150.019.1684710-11
GAGTACT119350.019.09267212-13
TACATGG146450.019.0802862
GTGGTAT36700.018.2877461
ACATGGG147650.018.1230453
CAGAGTA203100.017.73114810-11
TGGTATC35750.016.9331512
TATGCCG25250.016.16065442-43
Produced by FastQC (version 0.11.5)