##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6261503
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.339880376963805	32.0	28.0	33.0	18.0	34.0
2	31.528018432635104	33.0	31.0	33.0	27.0	34.0
3	32.33032771844076	33.0	33.0	34.0	30.0	34.0
4	32.59907341735682	33.0	33.0	34.0	31.0	34.0
5	32.955514993764275	33.0	33.0	34.0	32.0	34.0
6	36.49750115906676	38.0	37.0	38.0	34.0	38.0
7	36.62140591484185	38.0	37.0	38.0	34.0	38.0
8	36.672899461998185	38.0	38.0	38.0	34.0	38.0
9	36.91807749672882	38.0	38.0	38.0	35.0	38.0
10-11	37.05478077707541	38.0	38.0	38.0	36.0	38.0
12-13	37.11761233684628	38.0	38.0	38.0	36.0	38.0
14-15	37.11913409607885	38.0	38.0	38.0	36.0	38.0
16-17	37.08142733461918	38.0	38.0	38.0	36.0	38.0
18-19	37.121880720970665	38.0	38.0	38.0	36.0	38.0
20-21	37.09394197656711	38.0	38.0	38.0	36.0	38.0
22-23	37.137083700779286	38.0	38.0	38.0	36.0	38.0
24-25	37.14621052161964	38.0	38.0	38.0	36.0	38.0
26-27	37.08068107244471	38.0	38.0	38.0	36.0	38.0
28-29	37.07079160804017	38.0	38.0	38.0	36.0	38.0
30-31	36.941978095484444	38.0	38.0	38.0	36.0	38.0
32-33	37.03880515554789	38.0	38.0	38.0	36.0	38.0
34-35	37.05253409830334	38.0	38.0	38.0	36.0	38.0
36-37	36.978856192921505	38.0	38.0	38.0	36.0	38.0
38-39	37.01146748519602	38.0	38.0	38.0	36.0	38.0
40-41	36.93197597340794	38.0	38.0	38.0	35.5	38.0
42-43	36.916789249768954	38.0	38.0	38.0	35.5	38.0
44-45	36.91580880084126	38.0	38.0	38.0	36.0	38.0
46-47	36.875065117528166	38.0	38.0	38.0	35.0	38.0
48-49	36.83996109545993	38.0	38.0	38.0	35.0	38.0
50-51	36.85680371531952	38.0	38.0	38.0	35.0	38.0
52-53	36.76824211290679	38.0	38.0	38.0	35.0	38.0
54-55	36.754429456970705	38.0	38.0	38.0	35.0	38.0
56-57	36.75743484873516	38.0	38.0	38.0	35.0	38.0
58-59	36.77779065592148	38.0	38.0	38.0	35.0	38.0
60-61	36.77222892414731	38.0	38.0	38.0	35.0	38.0
62-63	36.77608119496738	38.0	38.0	38.0	35.0	38.0
64-65	36.7314252560799	38.0	38.0	38.0	35.0	38.0
66-67	36.733582065242246	38.0	38.0	38.0	35.0	38.0
68-69	36.742908954760715	38.0	38.0	38.0	35.0	38.0
70-71	36.65816801665829	38.0	38.0	38.0	34.0	38.0
72-73	36.61377640761449	38.0	38.0	38.0	34.0	38.0
74-75	36.66204244144784	38.0	38.0	38.0	34.0	38.0
76-77	36.63695732977474	38.0	38.0	38.0	34.0	38.0
78-79	36.65829316231533	38.0	38.0	38.0	34.0	38.0
80-81	36.67893196519131	38.0	38.0	38.0	34.0	38.0
82-83	36.55116400509496	38.0	38.0	38.0	34.0	38.0
84-85	36.56614029887088	38.0	38.0	38.0	34.0	38.0
86-87	36.56508517088919	38.0	38.0	38.0	34.0	38.0
88-89	36.51171289931505	38.0	38.0	38.0	34.0	38.0
90-91	36.508533696728875	38.0	38.0	38.0	34.0	38.0
92-93	36.55434125471909	38.0	38.0	38.0	34.0	38.0
94-95	36.4557999377255	38.0	38.0	38.0	34.0	38.0
96-97	36.415763412295775	38.0	38.0	38.0	34.0	38.0
98-99	36.48725465206731	38.0	38.0	38.0	34.0	38.0
100	34.896227735772705	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	17.0
20	41.0
21	162.0
22	646.0
23	1869.0
24	4651.0
25	9273.0
26	16202.0
27	25583.0
28	38653.0
29	53709.0
30	72761.0
31	95438.0
32	126025.0
33	167459.0
34	241569.0
35	402805.0
36	1005386.0
37	3999254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.97262366559595	19.463873130780264	15.234153844532216	26.32934935909158
2	13.135264807826491	20.868152582534897	43.582826679153555	22.41375593048506
3	16.369632019660454	27.263549981529994	31.447146156441992	24.91967184236756
4	11.514615580316738	19.571147694091977	40.09808827049991	28.816148455091373
5	13.047081515870559	37.528115405765014	36.49704284146593	12.927760236898497
6	28.19671251455122	39.478205152980046	19.785728761928244	12.539353570540493
7	24.991012541238103	33.34876626266888	24.690078404498088	16.970142791594924
8	23.33329553623148	37.35202234990545	22.270244061210224	17.04443805265285
9	24.903258036170875	17.201751273097095	22.21173130664176	35.68325938409027
10-11	23.04872328576701	27.408874514633307	30.15036485648893	19.39203734311075
12-13	24.006153155240842	25.002599216194575	30.133188469286047	20.85805915927853
14-15	21.38928944057042	26.377372972591406	27.566712017865356	24.666625568972815
16-17	20.941984696006692	29.375982092478438	28.312020292891336	21.370012918623534
18-19	20.972400715930345	28.710862232278732	29.773913707299986	20.54282334449093
20-21	21.84754799595205	27.851311188106187	29.500213169830996	20.80092764611077
22-23	21.537921708902342	27.819996548604504	29.36349383440377	21.278587908089385
24-25	21.37705540162798	27.9293441290451	29.45178961235647	21.24181085697045
26-27	21.347143323346057	28.12362476616532	29.347456087960015	21.181775822528603
28-29	21.248449657051278	28.14061576594331	29.40439989845184	21.206534678553574
30-31	21.642564464602142	27.917470475245388	29.31121200972938	21.128753050423086
32-33	21.313408798976752	28.126431703187315	29.26744163531688	21.292717862519048
34-35	21.44212178557646	28.09817574212044	29.265069022681907	21.1946334496212
36-37	21.39182256673052	28.08676584416952	29.287737352009025	21.233674237090934
38-39	21.254513973973715	28.098859254291497	29.424347408334086	21.2222793634007
40-41	21.525442534903572	27.965596701331158	29.28214426100072	21.22681650276455
42-43	21.46599734772088	28.0188365614052	29.17526536402852	21.3399007268454
44-45	21.491293299818665	28.13278557970878	29.021517223963595	21.354403896508963
46-47	21.57336459286368	28.02047841015448	28.848451746222043	21.5577052507598
48-49	21.491890962704378	28.14738414952628	28.877924774051078	21.482800113718255
50-51	21.41944082578734	28.253375877438934	28.834562310172856	21.492620986600866
52-53	21.63486075974321	28.170426245206727	28.756519878183674	21.438193116866387
54-55	21.59405320340671	28.09762299445931	28.75713160444935	21.55119219768463
56-57	21.632988925824957	28.20224314802946	28.662355068925987	21.5024128572196
58-59	21.515653384677304	28.30305011204299	28.816107403539686	21.365189099740018
60-61	21.627874899597586	28.372471426197844	28.65618493831681	21.343468735887758
62-63	21.563730336448366	28.397592344411652	28.63964499632671	21.39903232281327
64-65	21.55667149875227	28.340277291991185	28.632453399694285	21.470597809562268
66-67	21.616202473382568	28.370019705640185	28.52293128916556	21.490846531811687
68-69	21.561661443762752	28.32520344910141	28.6214256217908	21.49170948534504
70-71	21.613996978688593	28.092558438674626	28.665588052535668	21.627856530101113
72-73	21.62821067467096	28.090226704071576	28.643869891576312	21.637692729681152
74-75	21.54164025309954	28.17526709180495	28.593808908393033	21.68928374670248
76-77	21.664514048480328	28.152318864735047	28.487653164801152	21.69551392198347
78-79	21.60900319667094	28.19288708803883	28.482465721949808	21.71564399334042
80-81	21.619681150699606	28.19492104672613	28.476955443693008	21.708442358881257
82-83	21.678884985407485	28.19343628521122	28.487722557872225	21.639956171509073
84-85	21.78036097522185	28.153353148784444	28.46255968015525	21.60372619583846
86-87	21.631352418477015	28.16962541613661	28.494886701812373	21.704135463574
88-89	21.739488807230465	28.160950666675642	28.42771957025213	21.671840955841756
90-91	21.733026876348973	28.177569637412603	28.493553170484525	21.5958503157539
92-93	21.709402963378512	28.20417015035035	28.422370529068836	21.664056357202302
94-95	21.644536794606676	28.252904119433886	28.43543707747722	21.66712200848222
96-97	21.727504958996043	28.38178719182782	28.508175238717325	21.382532610458814
98-99	22.758881808896486	30.27456769088851	28.756888922146306	18.209661578068694
100	21.65460980744586	31.214391528103935	22.769413523938393	24.361585140511814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	677.0
1	711.0
2	1222.0
3	1832.5
4	2362.0
5	3376.5
6	4228.0
7	5009.0
8	5948.0
9	6482.0
10	6420.0
11	6479.5
12	7206.0
13	8714.5
14	11621.5
15	15116.5
16	17907.0
17	19537.5
18	19859.0
19	19289.5
20	18583.0
21	18654.0
22	20727.5
23	25358.5
24	32553.0
25	42581.0
26	56121.5
27	71235.0
28	86398.0
29	102310.5
30	117961.5
31	133805.0
32	150661.0
33	166971.5
34	182892.5
35	197978.5
36	212382.5
37	225502.0
38	234488.5
39	241994.0
40	250600.0
41	256824.5
42	260277.5
43	264902.5
44	268026.0
45	267358.5
46	263015.5
47	257059.0
48	250081.5
49	240079.5
50	229381.5
51	216922.5
52	202408.0
53	187065.5
54	170939.5
55	154865.5
56	138752.0
57	122779.5
58	107147.0
59	90991.0
60	74061.5
61	58113.0
62	44277.5
63	32902.5
64	23804.5
65	16891.0
66	12114.0
67	8931.5
68	6455.5
69	4481.5
70	3199.0
71	2226.5
72	1506.5
73	988.5
74	640.0
75	394.0
76	237.0
77	147.5
78	91.5
79	51.5
80	28.5
81	19.0
82	14.0
83	9.5
84	6.0
85	4.5
86	3.5
87	2.5
88	2.5
89	2.5
90	1.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.004088475243084607
6	0.0
7	0.0
8	0.0
9	1.5970606418299248E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.3965104890071663E-5
30-31	0.0
32-33	3.200453184170879E-5
34-35	0.0
36-37	4.5781683696114356E-4
38-39	2.4181997582928733E-5
40-41	2.1851229706277387E-4
42-43	4.063174893349786E-5
44-45	6.697008470817336E-4
46-47	0.0
48-49	4.13220237709074E-5
50-51	1.6620519793460126E-5
52-53	1.252977806171183E-4
54-55	0.0021497220980347172
56-57	0.001047368065409825
58-59	1.9546296465477203E-4
60-61	5.985842115205056E-4
62-63	8.603555970132238E-5
64-65	7.358891311166564E-4
66-67	0.0010809435835493987
68-69	4.3033662423752476E-4
70-71	6.637672134751471E-4
72-73	1.42721226374954E-4
74-75	2.066070416710234E-4
76-77	9.048415265074682E-6
78-79	0.0016320443799366494
80-81	0.0
82-83	0.001000302128290971
84-85	1.9600122043426593E-4
86-87	1.8814277214264042E-5
88-89	7.588087499375169E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.767758109012305E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	33.0
20-21	159.0
22-23	335.0
24-25	508.0
26-27	955.0
28-29	2108.0
30-31	6414.0
32-33	8630.0
34-35	12373.0
36-37	20743.0
38-39	25039.0
40-41	24582.0
42-43	29052.0
44-45	35314.0
46-47	36644.0
48-49	33995.0
50-51	31334.0
52-53	30830.0
54-55	33838.0
56-57	36155.0
58-59	36115.0
60-61	36040.0
62-63	35708.0
64-65	38519.0
66-67	42192.0
68-69	42368.0
70-71	46330.0
72-73	39317.0
74-75	39734.0
76-77	41538.0
78-79	42744.0
80-81	43055.0
82-83	41442.0
84-85	41628.0
86-87	43199.0
88-89	45173.0
90-91	45861.0
92-93	46646.0
94-95	57888.0
96-97	196194.0
98-99	447657.0
100-101	4443114.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.65383330428308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.63541407076899	81.2383072379891
2	6.185941866712125	10.968149181629649
3	1.339624007170574	3.5628841026634746
4	0.41818650077578645	1.482953453195121
5	0.1830519953522406	0.8114130540986969
6	0.08865445262490193	0.47157342388143103
7	0.04602078131696556	0.2855943072784991
8	0.03101988433197486	0.22000253237480336
9	0.016722366121899025	0.13342516727616244
>10	0.05432418277415953	0.6838292654286642
>50	6.35381546530238E-4	0.03849957042373145
>100	3.553677574224933E-4	0.06825375943461334
>500	4.91427463978504E-5	0.03511494432599696
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5970606418299248E-5	0.0	0.0	0.0
3	0.0	3.1941212836598495E-5	0.0	1.5970606418299248E-5	0.0
4	0.0	4.791181925489774E-5	0.0	1.5970606418299248E-5	0.0
5	0.0	4.791181925489774E-5	0.0	1.5970606418299248E-5	0.0
6	0.0	4.791181925489774E-5	0.0	3.1941212836598495E-5	3.1941212836598495E-5
7	0.0	4.791181925489774E-5	0.0	3.1941212836598495E-5	3.1941212836598495E-5
8	0.0	4.791181925489774E-5	0.0	3.1941212836598495E-5	3.1941212836598495E-5
9	0.0	4.791181925489774E-5	0.0	4.791181925489774E-5	4.791181925489774E-5
10-11	0.0	4.791181925489774E-5	0.0	4.791181925489774E-5	4.791181925489774E-5
12-13	0.0	4.791181925489774E-5	0.0	4.791181925489774E-5	4.791181925489774E-5
14-15	0.0	8.783833530064586E-5	0.0	6.388242567319699E-5	5.5897122464047363E-5
16-17	0.0	9.582363850979549E-5	0.0	8.783833530064586E-5	6.388242567319699E-5
18-19	0.0	1.0380894171894511E-4	0.0	1.1977954813724437E-4	6.388242567319699E-5
20-21	0.0	1.1179424492809474E-4	0.0	1.597060641829925E-4	6.388242567319699E-5
22-23	0.0	1.2776485134639398E-4	0.0	1.7567667060129174E-4	6.388242567319699E-5
24-25	0.0	1.4373545776469324E-4	0.0	2.0761788343789023E-4	6.388242567319699E-5
26-27	0.0	1.4373545776469324E-4	0.0	2.315737930653391E-4	6.388242567319699E-5
28-29	0.0	1.4373545776469324E-4	0.0	3.4336803799343385E-4	6.388242567319699E-5
30-31	0.0	2.2358848985618948E-4	0.0	8.06515624124112E-4	6.388242567319699E-5
32-33	0.0	2.2358848985618948E-4	0.0	0.001972369892659957	7.985303209149625E-5
34-35	0.0	2.8747091552938647E-4	0.0	0.00361734235374478	9.582363850979549E-5
36-37	0.0	3.3538273478428426E-4	0.0	0.006611831057175889	9.582363850979549E-5
38-39	0.0	3.912798572483316E-4	0.0	0.011187409796018623	9.582363850979549E-5
40-41	0.0	4.631475861306782E-4	0.0	0.01614628308890054	9.582363850979549E-5
42-43	0.0	4.7911819254897747E-4	0.0	0.02079372955662562	9.582363850979549E-5
44-45	0.0	5.509859214313241E-4	0.0	0.02651120665437675	9.582363850979549E-5
46-47	0.0	6.068830438953714E-4	0.0	0.03304318467946114	9.582363850979549E-5
48-49	0.0	6.068830438953714E-4	0.0	0.03922380936334295	1.1179424492809474E-4
50-51	0.0	6.547948631502692E-4	0.0	0.04542838995685221	1.1179424492809474E-4
52-53	0.0	6.547948631502692E-4	0.0	0.05125766129953144	1.1179424492809474E-4
54-55	0.0	6.707654695685684E-4	0.0	0.05718275628072046	1.1179424492809474E-4
56-57	0.0	6.78750772777718E-4	0.0	0.06147086410403381	1.1179424492809474E-4
58-59	0.0	6.867360759868677E-4	0.0	0.06566314828883736	1.1179424492809474E-4
60-61	0.0	6.867360759868677E-4	0.0	0.07007902096349711	1.1179424492809474E-4
62-63	0.0	6.867360759868677E-4	0.0	0.07429526105792811	1.1179424492809474E-4
64-65	0.0	7.506185016600647E-4	0.0	0.07851150115235911	1.1179424492809474E-4
66-67	0.0	8.06515624124112E-4	0.0	0.0830790945879927	1.2776485134639398E-4
68-69	0.0	8.145009273332617E-4	0.0	0.08729533468242369	1.5172076097384285E-4
70-71	0.0	8.145009273332617E-4	0.0	0.09131194219662594	1.7567667060129174E-4
72-73	0.0	8.224862305424113E-4	0.0	0.09454598999633154	1.7567667060129174E-4
74-75	0.0	8.544274433790098E-4	0.0	0.09685374262377579	1.7567667060129174E-4
76-77	0.0	9.023392626339075E-4	0.0	0.09958471632130497	1.7567667060129174E-4
78-79	0.0	9.262951722613564E-4	0.0	0.10127760060164467	1.7567667060129174E-4
80-81	1.5970606418299248E-5	9.342804754705061E-4	0.0	0.10195635137442241	1.7567667060129174E-4
82-83	1.5970606418299248E-5	9.422657786796557E-4	0.0	0.10238755774771649	1.7567667060129174E-4
84-85	1.5970606418299248E-5	9.582363850979549E-4	0.0	0.10272294048250077	1.7567667060129174E-4
86-87	1.5970606418299248E-5	9.821922947254038E-4	0.0	0.10281876412101056	1.7567667060129174E-4
88	1.5970606418299248E-5	0.001086001236444349	0.0	0.10281876412101056	1.7567667060129174E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6695	0.0	45.603004	1
GTATCAA	11880	0.0	35.96477	1
ATCAACG	13615	0.0	30.608408	3
TCAACGC	13710	0.0	30.5243	4
CAACGCA	13980	0.0	29.903397	5
AACGCAG	14505	0.0	28.90971	6
TATCAAC	15075	0.0	28.109594	2
ACGCAGA	16240	0.0	25.794134	7
CGCAGAG	16435	0.0	25.488085	8
CTTATAC	4290	0.0	20.348415	1
GCAGAGT	20720	0.0	20.153515	9
GTACATG	14565	0.0	19.335676	1
AGAGTAC	18215	0.0	19.16847	10-11
GAGTACT	11935	0.0	19.092672	12-13
TACATGG	14645	0.0	19.080286	2
GTGGTAT	3670	0.0	18.287746	1
ACATGGG	14765	0.0	18.123045	3
CAGAGTA	20310	0.0	17.731148	10-11
TGGTATC	3575	0.0	16.933151	2
TATGCCG	2525	0.0	16.160654	42-43
>>END_MODULE