FastQCFastQC Report
Fri 27 May 2016
SRR2138970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5564651
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.050.6040231
GTATCAA108950.038.1185871
TCAACGC129400.031.4201834
ATCAACG129400.031.386473
CAACGCA132650.030.6832565
TATCAAC138350.029.7028962
AACGCAG137800.029.5352046
CTTATAC41450.028.5214651
ACGCAGA155250.026.2435677
CGCAGAG165100.024.651438
GTACATG153800.021.6702581
TACATGG152350.021.217922
GCAGAGT193750.021.1412899
ACATGGG151600.020.488393
AGAGTAC171200.019.70897910-11
CGTACTA29600.019.68532828-29
TATACAC69400.019.6748733
TTATACA68800.019.6562352
GAGTACT105700.019.64443412-13
TATGCCG31200.019.50490842-43
Produced by FastQC (version 0.11.5)