##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5564651
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.645343436632412	32.0	30.0	33.0	18.0	34.0
2	31.59106420151057	33.0	31.0	33.0	28.0	34.0
3	32.34094123782426	33.0	33.0	34.0	30.0	34.0
4	32.59684821204421	33.0	33.0	34.0	31.0	34.0
5	32.933379649505426	33.0	33.0	34.0	32.0	34.0
6	36.49267905570358	38.0	37.0	38.0	34.0	38.0
7	36.620379067797785	38.0	37.0	38.0	34.0	38.0
8	36.66841622232913	38.0	38.0	38.0	34.0	38.0
9	36.90555148921289	38.0	38.0	38.0	35.0	38.0
10-11	37.03367048535479	38.0	38.0	38.0	36.0	38.0
12-13	37.092065612021315	38.0	38.0	38.0	36.0	38.0
14-15	37.09635968185606	38.0	38.0	38.0	36.0	38.0
16-17	37.05846215692593	38.0	38.0	38.0	36.0	38.0
18-19	37.09621744472385	38.0	38.0	38.0	36.0	38.0
20-21	37.06743553886754	38.0	38.0	38.0	36.0	38.0
22-23	37.11175611765946	38.0	38.0	38.0	36.0	38.0
24-25	37.11815041838024	38.0	38.0	38.0	36.0	38.0
26-27	37.053823566386036	38.0	38.0	38.0	36.0	38.0
28-29	37.04183400298518	38.0	38.0	38.0	36.0	38.0
30-31	36.91217270523565	38.0	38.0	38.0	35.5	38.0
32-33	37.006561291905044	38.0	38.0	38.0	36.0	38.0
34-35	37.021660670167236	38.0	38.0	38.0	36.0	38.0
36-37	36.94675070390894	38.0	38.0	38.0	36.0	38.0
38-39	36.97585758241122	38.0	38.0	38.0	36.0	38.0
40-41	36.904522203107135	38.0	38.0	38.0	35.5	38.0
42-43	36.889735776474765	38.0	38.0	38.0	35.0	38.0
44-45	36.88806450139307	38.0	38.0	38.0	35.5	38.0
46-47	36.84701653172543	38.0	38.0	38.0	35.0	38.0
48-49	36.81167551718289	38.0	38.0	38.0	35.0	38.0
50-51	36.82864799892707	38.0	38.0	38.0	35.0	38.0
52-53	36.741104046721	38.0	38.0	38.0	35.0	38.0
54-55	36.723630071936796	38.0	38.0	38.0	35.0	38.0
56-57	36.72887421705041	38.0	38.0	38.0	35.0	38.0
58-59	36.75048099401232	38.0	38.0	38.0	35.0	38.0
60-61	36.749141837669484	38.0	38.0	38.0	35.0	38.0
62-63	36.75199660058152	38.0	38.0	38.0	35.0	38.0
64-65	36.709787439485034	38.0	38.0	38.0	35.0	38.0
66-67	36.712023337676314	38.0	38.0	38.0	35.0	38.0
68-69	36.721839461760496	38.0	38.0	38.0	35.0	38.0
70-71	36.63780278389153	38.0	38.0	38.0	34.0	38.0
72-73	36.59219203569498	38.0	38.0	38.0	34.0	38.0
74-75	36.64094127378661	38.0	38.0	38.0	34.0	38.0
76-77	36.617182774463515	38.0	38.0	38.0	34.0	38.0
78-79	36.63470997321256	38.0	38.0	38.0	34.0	38.0
80-81	36.65776065277837	38.0	38.0	38.0	34.0	38.0
82-83	36.528702225456016	38.0	38.0	38.0	34.0	38.0
84-85	36.543423252540464	38.0	38.0	38.0	34.0	38.0
86-87	36.54085086619859	38.0	38.0	38.0	34.0	38.0
88-89	36.48976116777398	38.0	38.0	38.0	34.0	38.0
90-91	36.48725104106856	38.0	38.0	38.0	34.0	38.0
92-93	36.53044580870258	38.0	38.0	38.0	34.0	38.0
94-95	36.43529059284553	38.0	38.0	38.0	34.0	38.0
96-97	36.39761128537008	38.0	38.0	38.0	34.0	38.0
98-99	36.47074823421396	38.0	38.0	38.0	34.0	38.0
100	34.878956563297734	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	8.0
20	36.0
21	156.0
22	605.0
23	1916.0
24	4455.0
25	8873.0
26	15076.0
27	23955.0
28	35343.0
29	49589.0
30	66576.0
31	87667.0
32	114286.0
33	152091.0
34	217515.0
35	361689.0
36	895664.0
37	3529144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.292059825494896	19.436205433188892	15.108782203951336	26.162952537364877
2	13.401972558566566	20.785310705019956	43.07702315922418	22.7356935771893
3	16.488653106906433	27.17133563272881	31.269921509902414	25.070089750462337
4	11.729702365880627	19.425746556253035	39.40985697036526	29.43469410750108
5	13.292318065887049	37.34120571515655	36.1871076868223	13.179368532134097
6	28.63463110887477	38.96223482159705	19.494631288580592	12.90850278094759
7	25.353539691887235	33.07093293002562	24.39137692552507	17.184150452562076
8	23.80278655390967	37.00429730453895	21.838746041755357	17.354170099796015
9	25.13997733191174	16.963148272910555	21.94311916416681	35.953755231010895
10-11	23.406777891371807	27.204410483245038	29.66199497506672	19.726816650316433
12-13	24.319162154104543	24.807979871513954	29.658643462096723	21.214214512284777
14-15	21.705655934217617	26.046476230045695	27.128197258013127	25.119670577723564
16-17	21.31255850546602	29.10779130622927	27.858009424131	21.72164076417371
18-19	21.38605817327987	28.38899510499401	29.311460862505122	20.913485859221
20-21	22.216573411876357	27.485224212694064	29.11236912453713	21.18583325089245
22-23	21.991227504238076	27.515236492029654	28.880612719669575	21.612923284062703
24-25	21.83344748737401	27.64158171311577	29.00252492802544	21.522445871484777
26-27	21.693621253943256	27.773155485001933	28.978774060112332	21.55444920094248
28-29	21.567923313091615	27.67758095375926	29.06838512267505	21.686110610474067
30-31	21.923707778402925	27.44972851079638	29.126156915497663	21.500406795303032
32-33	21.738313862630545	27.671403752502695	28.943910630145282	21.64637175472148
34-35	21.815181586658262	27.707275135629654	29.00801842395511	21.46952485375697
36-37	21.91247176591794	27.64317804656087	28.833630315054066	21.610719872467126
38-39	21.71436109863256	27.687427611901693	29.012954327229707	21.58525696223604
40-41	21.82448109743235	27.53829513876549	28.956660342499035	21.680563421303123
42-43	21.84162894099813	27.62726170401532	28.828647369534306	21.70246198545224
44-45	21.784365975058602	27.719292543849	28.718512450062317	21.77782903103008
46-47	21.963878738913404	27.616127457190785	28.454432654454347	21.965561149441463
48-49	21.940842952603155	27.737116385172516	28.460534755455413	21.86150590676891
50-51	21.831100212347646	27.830429541741054	28.46969423671396	21.868776009197337
52-53	22.020224187018865	27.838443564471543	28.374840559216917	21.766491689292668
54-55	21.9708691432684	27.754205194867264	28.33531419198343	21.939611469880905
56-57	22.05437771279335	27.930947148041884	28.163028918070577	21.85164622109419
58-59	21.829793994863802	27.948285944947166	28.456101977372427	21.765818082816608
60-61	22.006318488337033	28.056760624349792	28.240597835310776	21.696323052002402
62-63	21.924354727783115	28.183114534933296	28.240704971013823	21.65182576626976
64-65	21.97374160972374	28.034187681254103	28.171271505313705	21.82079920370845
66-67	21.986126703788074	28.064313364518906	28.05958027605194	21.889979655641078
68-69	21.946477656518418	28.00682157492692	28.15840325976453	21.88829750879013
70-71	21.970030648895385	27.822862239295542	28.21254406954285	21.994563042266225
72-73	22.04614934073819	27.735702270901097	28.192867795972187	22.025280592388523
74-75	21.968861301163567	27.83447473712454	28.12555006079001	22.071113900921876
76-77	22.066337376509765	27.83548529684388	28.037402492286212	22.060774834360142
78-79	21.98514905124348	27.805037066256023	28.044735572356323	22.165078310144175
80-81	22.003046558655857	27.822288810170004	28.079839292674002	22.094825338500137
82-83	22.1105409255768	27.794942706485188	28.110600241512618	21.983916126425395
84-85	22.12394490226441	27.786871316851812	28.068068265321433	22.021115515562347
86-87	22.008750806331008	27.81328795071675	28.14132843640582	22.03663280654642
88-89	22.1228713080078	27.750913449330998	28.04316343964779	22.083051803013415
90-91	22.109391670755567	27.8739074019009	28.00821995886115	22.008480968482385
92-93	22.15164498646439	27.817533840116926	28.055209664382307	21.975611509036376
94-95	22.0021371200982	27.9368622308178	28.012102987994155	22.048897661089843
96-97	22.119739567694193	28.048231074221686	28.066838499860836	21.765190858223285
98-99	23.239489339916418	29.88990355996199	28.3147927450817	18.55581435503989
100	21.988153510609425	30.826964204975262	22.381828064298134	24.80305422011718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	718.0
1	734.5
2	1163.5
3	1711.0
4	2213.5
5	3117.5
6	3847.0
7	4619.5
8	5497.5
9	5877.0
10	5833.0
11	5952.0
12	6704.5
13	8028.0
14	10650.0
15	13757.5
16	16087.5
17	17585.0
18	17834.5
19	17093.5
20	16334.0
21	16400.0
22	18062.5
23	21866.5
24	27686.0
25	35734.0
26	45908.5
27	57412.0
28	70190.5
29	84258.5
30	98399.0
31	112069.0
32	125620.5
33	138864.5
34	151745.0
35	164242.0
36	176271.5
37	186812.5
38	195998.5
39	203686.0
40	210870.5
41	217121.5
42	220946.0
43	226946.5
44	233089.5
45	235950.5
46	235614.5
47	233453.0
48	229225.0
49	222243.5
50	215303.0
51	207867.0
52	196493.0
53	181631.5
54	167162.5
55	153086.0
56	139065.0
57	124393.0
58	109412.0
59	93703.0
60	76812.0
61	62042.0
62	49036.0
63	37523.0
64	28647.5
65	21106.0
66	15409.0
67	11145.5
68	7616.5
69	5291.0
70	3801.0
71	2716.5
72	1853.5
73	1165.5
74	730.0
75	474.5
76	289.0
77	163.0
78	98.5
79	60.5
80	38.0
81	23.5
82	17.5
83	13.0
84	10.0
85	9.0
86	7.0
87	5.0
88	4.0
89	3.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0039355567851425
6	1.7970578927591327E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.7972293911705713E-5
26-27	0.0
28-29	4.49449514234965E-5
30-31	0.0
32-33	5.4046472940687505E-5
34-35	0.0
36-37	4.7973043856142057E-4
38-39	5.455101378967802E-5
40-41	2.0098917737230952E-4
42-43	6.426334605104951E-5
44-45	6.92468089223867E-4
46-47	0.0
48-49	1.1228251928662799E-4
50-51	9.414241205098187E-6
52-53	1.0413994135974574E-4
54-55	0.0021326742354386666
56-57	0.0012165274157863687
58-59	1.446108036417628E-4
60-61	3.39629481702122E-4
62-63	1.4651548092339134E-4
64-65	6.783848306073077E-4
66-67	0.0012575202433529411
68-69	2.8935335312643305E-4
70-71	7.342564848426327E-4
72-73	1.9266542988981058E-4
74-75	1.6335885407031046E-4
76-77	0.0
78-79	0.0016677102803980338
80-81	0.0
82-83	9.254883055087378E-4
84-85	1.4832158237939602E-4
86-87	1.0674577809773047E-5
88-89	5.3795573592613655E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.910900471386744E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	111.0
22-23	315.0
24-25	399.0
26-27	992.0
28-29	2373.0
30-31	7362.0
32-33	9866.0
34-35	14014.0
36-37	22996.0
38-39	26793.0
40-41	26094.0
42-43	29336.0
44-45	35520.0
46-47	36280.0
48-49	33374.0
50-51	30226.0
52-53	29341.0
54-55	31149.0
56-57	33459.0
58-59	33309.0
60-61	34047.0
62-63	33202.0
64-65	35291.0
66-67	37686.0
68-69	39008.0
70-71	42635.0
72-73	33889.0
74-75	33852.0
76-77	35623.0
78-79	36651.0
80-81	36419.0
82-83	34862.0
84-85	35080.0
86-87	36574.0
88-89	37696.0
90-91	38451.0
92-93	39662.0
94-95	49572.0
96-97	173873.0
98-99	400292.0
100-101	3916941.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.75572536050511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.83921949437831	81.51256542766188
2	6.0198452252354135	10.685914590474848
3	1.2901899156034145	3.435352254365698
4	0.42249110923411176	1.4999401943375197
5	0.17101015320066737	0.7589065095668168
6	0.0908322220960777	0.48371278549463265
7	0.054162998105843234	0.3365093329208647
8	0.025977809077298464	0.1844543430345871
9	0.020902275810250535	0.16696769861017133
>10	0.0643992195514767	0.8477718248191388
>50	7.37921391184838E-4	0.04319532259749677
>100	2.3165631595445848E-4	0.04470971611634649
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.5941157855182654E-5	0.0
4	0.0	5.391173678277398E-5	0.0	3.5941157855182654E-5	0.0
5	0.0	5.391173678277398E-5	0.0	3.5941157855182654E-5	0.0
6	0.0	5.391173678277398E-5	0.0	3.5941157855182654E-5	0.0
7	0.0	7.188231571036531E-5	0.0	3.5941157855182654E-5	0.0
8	0.0	7.188231571036531E-5	0.0	3.5941157855182654E-5	0.0
9	0.0	1.4376463142073062E-4	0.0	3.5941157855182654E-5	0.0
10-11	0.0	1.4376463142073062E-4	0.0	3.5941157855182654E-5	0.0
12-13	0.0	1.6173521034832194E-4	0.0	5.391173678277398E-5	0.0
14-15	0.0	2.3361752605868726E-4	0.0	1.1680876302934363E-4	0.0
16-17	0.0	2.605733944500742E-4	0.0	1.5274992088452628E-4	0.0
18-19	0.0	3.0549984176905256E-4	0.0	2.0666165766730025E-4	8.985289463795664E-6
20-21	0.0	3.6839686801562216E-4	0.0	2.3361752605868724E-4	1.7970578927591327E-5
22-23	1.7970578927591327E-5	4.133233153346005E-4	0.0	2.515881049862786E-4	1.7970578927591327E-5
24-25	1.7970578927591327E-5	4.312938942621918E-4	0.0	2.785439733776656E-4	1.7970578927591327E-5
26-27	1.7970578927591327E-5	4.4926447318978315E-4	0.0	3.1448513123284825E-4	1.7970578927591327E-5
28-29	1.7970578927591327E-5	4.5824976265357884E-4	0.0	4.4027918372598746E-4	1.7970578927591327E-5
30-31	1.7970578927591327E-5	5.750585256829225E-4	0.0	0.0010422935778002969	1.7970578927591327E-5
32-33	1.7970578927591327E-5	5.930291046105137E-4	0.0	0.0025518222077179683	3.5941157855182654E-5
34-35	1.7970578927591327E-5	6.469408413932877E-4	0.0	0.0043668506794046925	3.5941157855182654E-5
36-37	1.7970578927591327E-5	7.098378676398574E-4	0.0	0.007511701991733175	3.5941157855182654E-5
38-39	1.7970578927591327E-5	8.086760517416096E-4	0.0	0.012318831854863856	3.5941157855182654E-5
40-41	1.7970578927591327E-5	8.985289463795663E-4	0.0	0.017449432138691177	3.5941157855182654E-5
42-43	1.7970578927591327E-5	9.344701042347489E-4	0.0	0.022481194238416748	3.5941157855182654E-5
44-45	1.7970578927591327E-5	0.0010512788672640927	0.0	0.028007147258651083	3.5941157855182654E-5
46-47	1.7970578927591327E-5	0.0011321464724382536	0.0	0.03394642359422001	5.391173678277398E-5
48-49	1.7970578927591327E-5	0.0011321464724382536	0.0	0.04031699382405114	5.391173678277398E-5
50-51	1.7970578927591327E-5	0.0013208375511779625	0.0	0.04596874089677861	5.391173678277398E-5
52-53	1.7970578927591327E-5	0.001329822840641758	0.0	0.05183613491663718	5.391173678277398E-5
54-55	1.7970578927591327E-5	0.0013388081301055539	0.0	0.057784396541669905	5.391173678277398E-5
56-57	1.7970578927591327E-5	0.0013657639984969408	0.0	0.06356193766689053	6.289702624656965E-5
58-59	1.7970578927591327E-5	0.0013747492879607366	0.0	0.06907890539766107	7.188231571036531E-5
60-61	1.7970578927591327E-5	0.0014017051563521234	0.0	0.0739399469975745	7.188231571036531E-5
62-63	1.7970578927591327E-5	0.0014196757352797147	0.0	0.07864823867660344	7.188231571036531E-5
64-65	1.7970578927591327E-5	0.0016173521034832192	0.0	0.08341942738187894	8.086760517416097E-5
66-67	1.7970578927591327E-5	0.0016353226824108106	0.0	0.08797496914002334	8.985289463795663E-5
68-69	2.6955868391386992E-5	0.0016622785508021978	0.0	0.0928180401610092	8.985289463795663E-5
70-71	3.5941157855182654E-5	0.0017161902875849717	0.0	0.09651099413062922	8.985289463795663E-5
72-73	3.5941157855182654E-5	0.0017431461559763587	0.0	0.09964686015349392	8.985289463795663E-5
74-75	3.5941157855182654E-5	0.0018060431822229284	0.0	0.10203694715086355	8.985289463795663E-5
76-77	3.5941157855182654E-5	0.0018869107873970893	0.0	0.1046157252269729	8.985289463795663E-5
78-79	3.5941157855182654E-5	0.0019048813663246806	0.0	0.10612525385689058	8.985289463795663E-5
80-81	3.5941157855182654E-5	0.0019048813663246806	0.0	0.10651162130383379	8.985289463795663E-5
82-83	3.5941157855182654E-5	0.0019048813663246806	0.0	0.10678117998774767	8.985289463795663E-5
84-85	3.5941157855182654E-5	0.0019408225241798632	0.0	0.10696987106648737	8.985289463795663E-5
86-87	3.5941157855182654E-5	0.0019587931031074543	0.0	0.10706870925058912	8.985289463795663E-5
88	3.5941157855182654E-5	0.0020666165766730026	0.0	0.10706870925058912	8.985289463795663E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5750	0.0	50.604023	1
GTATCAA	10895	0.0	38.118587	1
TCAACGC	12940	0.0	31.420183	4
ATCAACG	12940	0.0	31.38647	3
CAACGCA	13265	0.0	30.683256	5
TATCAAC	13835	0.0	29.702896	2
AACGCAG	13780	0.0	29.535204	6
CTTATAC	4145	0.0	28.521465	1
ACGCAGA	15525	0.0	26.243567	7
CGCAGAG	16510	0.0	24.65143	8
GTACATG	15380	0.0	21.670258	1
TACATGG	15235	0.0	21.21792	2
GCAGAGT	19375	0.0	21.141289	9
ACATGGG	15160	0.0	20.48839	3
AGAGTAC	17120	0.0	19.708979	10-11
CGTACTA	2960	0.0	19.685328	28-29
TATACAC	6940	0.0	19.674873	3
TTATACA	6880	0.0	19.656235	2
GAGTACT	10570	0.0	19.644434	12-13
TATGCCG	3120	0.0	19.504908	42-43
>>END_MODULE