FastQCFastQC Report
Fri 27 May 2016
SRR2138969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8279705
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97500.051.031771
CTTATAC96900.050.0472451
TATACAC140750.037.1721653
GTATCAA183400.036.6547931
TTATACA138200.036.3173142
TCAACGC216350.030.8312594
ATCAACG216000.030.8007433
CAACGCA221000.030.1432195
TATCAAC229900.029.278732
AACGCAG230450.028.9432896
ACATCTC176600.027.9268118
ACACATC176050.027.8165996
TACACAT187750.027.5890065
ACGCAGA256800.026.0411287
CACATCT191200.025.6125137
CGCAGAG267350.025.0947728
CATCTCC200500.024.3594869
ATACACA231650.023.4861494
GCAGAGT311350.021.5344229
GTACATG251250.021.1005381
Produced by FastQC (version 0.11.5)