##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8279705
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.086457911242007	32.0	27.0	33.0	18.0	33.0
2	31.420663658910552	33.0	31.0	33.0	27.0	34.0
3	32.26627760288561	33.0	33.0	34.0	29.0	34.0
4	32.54887945886961	33.0	33.0	34.0	31.0	34.0
5	32.92324460835259	33.0	33.0	34.0	32.0	34.0
6	36.45255380475512	38.0	37.0	38.0	34.0	38.0
7	36.57218813955328	38.0	37.0	38.0	34.0	38.0
8	36.624423333923126	38.0	37.0	38.0	34.0	38.0
9	36.88405178686922	38.0	38.0	38.0	35.0	38.0
10-11	37.01913951040526	38.0	38.0	38.0	36.0	38.0
12-13	37.084316228657904	38.0	38.0	38.0	36.0	38.0
14-15	37.090542778999975	38.0	38.0	38.0	36.0	38.0
16-17	37.04632659013818	38.0	38.0	38.0	36.0	38.0
18-19	37.09070395623999	38.0	38.0	38.0	36.0	38.0
20-21	37.0630933996326	38.0	38.0	38.0	36.0	38.0
22-23	37.109927539643735	38.0	38.0	38.0	36.0	38.0
24-25	37.11719452917152	38.0	38.0	38.0	36.0	38.0
26-27	37.052887045611044	38.0	38.0	38.0	36.0	38.0
28-29	37.038455325579335	38.0	38.0	38.0	36.0	38.0
30-31	36.90534689449754	38.0	38.0	38.0	35.5	38.0
32-33	36.99469541694698	38.0	38.0	38.0	36.0	38.0
34-35	37.00206870123748	38.0	38.0	38.0	36.0	38.0
36-37	36.91758619839004	38.0	38.0	38.0	36.0	38.0
38-39	36.92560778812654	38.0	38.0	38.0	36.0	38.0
40-41	36.89585697005812	38.0	38.0	38.0	35.5	38.0
42-43	36.88649137782473	38.0	38.0	38.0	35.0	38.0
44-45	36.880202304293405	38.0	38.0	38.0	35.0	38.0
46-47	36.83331824798988	38.0	38.0	38.0	35.0	38.0
48-49	36.80015134744491	38.0	38.0	38.0	35.0	38.0
50-51	36.81477808373165	38.0	38.0	38.0	35.0	38.0
52-53	36.72318344837639	38.0	38.0	38.0	35.0	38.0
54-55	36.70469186490351	38.0	38.0	38.0	35.0	38.0
56-57	36.70667498858644	38.0	38.0	38.0	35.0	38.0
58-59	36.72779558422798	38.0	38.0	38.0	35.0	38.0
60-61	36.71784970809932	38.0	38.0	38.0	35.0	38.0
62-63	36.722221382222244	38.0	38.0	38.0	35.0	38.0
64-65	36.678230192921035	38.0	38.0	38.0	34.5	38.0
66-67	36.679932353973264	38.0	38.0	38.0	34.0	38.0
68-69	36.686931610731456	38.0	38.0	38.0	34.5	38.0
70-71	36.601086630979374	38.0	38.0	38.0	34.0	38.0
72-73	36.55346170704706	38.0	38.0	38.0	34.0	38.0
74-75	36.60607525021641	38.0	38.0	38.0	34.0	38.0
76-77	36.58528823322073	38.0	38.0	38.0	34.0	38.0
78-79	36.604923160467806	38.0	38.0	38.0	34.0	38.0
80-81	36.627436955134954	38.0	38.0	38.0	34.0	38.0
82-83	36.49831338516343	38.0	38.0	38.0	34.0	38.0
84-85	36.51517251268161	38.0	38.0	38.0	34.0	38.0
86-87	36.51036701734527	38.0	38.0	38.0	34.0	38.0
88-89	36.46103636603734	38.0	38.0	38.0	34.0	38.0
90-91	36.45686254254265	38.0	38.0	38.0	34.0	38.0
92-93	36.50346893510707	38.0	38.0	38.0	34.0	38.0
94-95	36.40734729755276	38.0	38.0	38.0	34.0	38.0
96-97	36.370694732145964	38.0	37.5	38.0	34.0	38.0
98-99	36.441455196951594	38.0	37.5	38.0	34.0	38.0
100	34.81879436813185	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	16.0
20	72.0
21	231.0
22	957.0
23	2803.0
24	6762.0
25	13136.0
26	22916.0
27	36243.0
28	53435.0
29	75365.0
30	100918.0
31	132217.0
32	172628.0
33	230924.0
34	330557.0
35	555339.0
36	1383782.0
37	5161399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.77936472374318	19.416005763490364	14.932935412553949	26.87169410021251
2	13.175396949529	20.614055694013253	43.77605240766428	22.434494948793464
3	16.34707999862314	27.60908752183804	31.459381705024512	24.58445077451431
4	11.494443340674577	19.850453609156364	39.252268045781825	29.402835004387235
5	13.021582620851552	37.57039918264539	36.37246112124403	13.03555707525902
6	28.0456765121072	39.20119608140581	19.63567779717729	13.117449609309705
7	24.883471089851632	33.134320606833214	24.32939337814572	17.652814925169437
8	23.436475091805807	37.22740121779701	22.106355238501855	17.22976845189533
9	24.873836069502	16.952405544932525	21.834898928753972	36.3388594568115
10-11	23.207765252505975	27.28217973949555	29.782437900867244	19.72761710713123
12-13	24.26338257220517	24.758243198278198	29.706444855221292	21.27192937429534
14-15	21.57092553418268	26.044714153463193	27.03233388146075	25.35202643089337
16-17	21.273819538256497	29.282860923185066	27.7114945520402	21.731824986518237
18-19	21.396172931281974	28.275137821939307	29.250353726370687	21.078335520408036
20-21	22.090985813000948	27.415321113521657	28.993068797207588	21.500624276269804
22-23	21.806738913628674	27.788624793415185	28.820199130673934	21.58443716228221
24-25	21.765018801699238	27.93997975303828	28.97093566884851	21.32406577641397
26-27	21.708646555685647	28.088144478596828	28.997532233896102	21.205676731821427
28-29	21.432434574736998	27.707712462336914	29.281955744847032	21.577897218079052
30-31	21.694456898298682	28.112901322430044	28.887996564187162	21.304645215084108
32-33	22.192267065358422	27.568915022765434	28.940041496488927	21.298776415387216
34-35	21.790769282278088	27.579992626954848	29.079778944509403	21.549459146257657
36-37	21.481989195902017	28.099493129066683	28.95879775820563	21.459719916825673
38-39	21.445763155166357	27.58067104787822	29.30222117980394	21.671344617151487
40-41	21.60294241334621	27.48320765292685	29.277111022569674	21.636738911157263
42-43	21.826905179619803	27.448386324798346	29.162959605417534	21.561748890164317
44-45	21.52416762634583	27.765723571819628	29.054106420543686	21.656002381290858
46-47	21.966983437116607	27.405712613563	28.59279276735308	22.03451118196731
48-49	21.88369647524673	27.4713335496839	28.635601492315782	22.009368482753587
50-51	21.58611150928548	27.639093909134605	28.845780580503423	21.929014001076492
52-53	21.72792087874374	27.652015718439866	29.053380140531072	21.56668326228533
54-55	21.709093683934512	27.676151588029956	28.639888292990705	21.97486643504482
56-57	22.01853223655817	27.983284270466303	28.193354933877856	21.804828559097672
58-59	21.482011140326147	28.278937864635108	28.833775794647465	21.405275200391277
60-61	21.87646318246224	28.717947044461166	28.064673399073612	21.34091637400298
62-63	21.536795569478993	29.091632257503136	28.061861309886993	21.309710863130878
64-65	21.598197232536666	28.907114923314463	28.031886174784397	21.462801669364474
66-67	21.627823087978033	28.8269186260737	27.980771837585223	21.564486448363045
68-69	21.653050211412143	28.638235783243243	28.096470673604173	21.61224333174044
70-71	21.751439677713428	28.21736278896745	28.24148097379932	21.7897165595198
72-73	21.88400692272395	27.922526903216287	28.29936194544737	21.894104228612385
74-75	21.818538730320757	27.982578139358903	28.28313164059908	21.91575148972126
76-77	21.917842641388198	27.931108098214217	28.184847057374217	21.966202203023364
78-79	21.861859048874464	27.938903535402552	28.203323736606528	21.995913679116455
80-81	21.824357905110094	27.982368386811913	28.222782023689412	21.970491684388577
82-83	21.97328776827813	27.85626969459664	28.286831946059245	21.88361059106598
84-85	22.01188653153454	27.919952311745067	28.174527515643554	21.89363364107684
86-87	21.895636189231553	27.92988984763818	28.2585293670845	21.915944596045772
88-89	21.975684834210007	27.907637628302172	28.142820204887148	21.97385733260067
90-91	22.006429439950253	27.962541201901814	28.144571713055242	21.886457645092694
92-93	22.04967302064875	27.91563060474153	28.175263441521896	21.859432933087824
94-95	21.883264869943684	27.98714317547705	28.166684306430128	21.962907648149134
96-97	21.976593723803003	28.09868230807136	28.22406460591726	21.70065936220838
98-99	23.0823858302489	30.00994551219388	28.480064781588677	18.427603875968547
100	21.924668903784003	30.933850205038066	22.46064178197895	24.680839109198978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1075.0
1	1146.0
2	1970.0
3	2897.0
4	3679.5
5	5242.5
6	6480.0
7	7664.5
8	9024.5
9	9551.0
10	9379.5
11	9420.0
12	10479.0
13	12610.0
14	16773.5
15	21949.0
16	26019.5
17	28417.0
18	28540.0
19	26955.0
20	25291.0
21	24865.0
22	26787.5
23	31959.0
24	40515.0
25	52624.0
26	68053.5
27	85465.0
28	104932.5
29	126039.0
30	146563.5
31	167252.0
32	188642.5
33	209093.5
34	229209.5
35	247216.5
36	265224.5
37	282727.5
38	296556.0
39	308271.5
40	317744.0
41	325771.5
42	334295.5
43	356693.0
44	379178.5
45	382459.5
46	370540.5
47	353052.0
48	339181.5
49	326538.5
50	315095.5
51	301338.0
52	283450.5
53	263225.5
54	242598.0
55	221866.5
56	200473.0
57	178567.0
58	156486.0
59	132797.0
60	107497.0
61	85503.5
62	66525.5
63	50347.0
64	37460.5
65	26826.5
66	19383.0
67	14033.0
68	9778.0
69	6650.0
70	4579.0
71	3227.5
72	2207.5
73	1436.5
74	897.0
75	545.0
76	340.0
77	203.5
78	134.5
79	91.5
80	54.0
81	35.5
82	27.5
83	19.0
84	14.5
85	11.0
86	5.5
87	4.0
88	5.0
89	4.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0037682501973198322
6	1.2077724991409718E-5
7	0.0
8	0.0
9	1.2077724991409718E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.2079083207247837E-5
26-27	0.0
28-29	1.812467711642912E-5
30-31	0.0
32-33	1.8161975398151365E-5
34-35	0.0
36-37	4.8662487207848674E-4
38-39	4.887606136658323E-5
40-41	1.6575911504769226E-4
42-43	8.637206184609794E-5
44-45	6.329219568135011E-4
46-47	0.0
48-49	7.547107156091534E-5
50-51	6.328598824171654E-6
52-53	5.7284766968432214E-5
54-55	0.0018244496611612886
56-57	0.0010241902793520856
58-59	9.076564582544807E-5
60-61	2.93696202605999E-4
62-63	1.1829710528297785E-4
64-65	6.156412191258411E-4
66-67	0.0010149785458909862
68-69	2.5626465935008584E-4
70-71	4.362795633086978E-4
72-73	1.793234168863206E-4
74-75	9.728215208132732E-5
76-77	3.499749942866582E-5
78-79	0.0015658859197089877
80-81	0.0
82-83	0.0010248305697068971
84-85	1.877714290171278E-4
86-87	0.0
88-89	7.339363354265199E-6
90-91	0.0
92-93	0.0
94-95	7.5383598865778375E-6
96-97	2.982734555744632E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	71.0
20-21	245.0
22-23	467.0
24-25	730.0
26-27	1467.0
28-29	3611.0
30-31	10918.0
32-33	14200.0
34-35	20417.0
36-37	33575.0
38-39	40018.0
40-41	39089.0
42-43	44245.0
44-45	53338.0
46-47	54468.0
48-49	50479.0
50-51	45950.0
52-53	44175.0
54-55	47531.0
56-57	50115.0
58-59	50467.0
60-61	53216.0
62-63	53691.0
64-65	62592.0
66-67	71244.0
68-69	73616.0
70-71	95485.0
72-73	56097.0
74-75	51586.0
76-77	53928.0
78-79	56195.0
80-81	56140.0
82-83	53804.0
84-85	53750.0
86-87	55763.0
88-89	58105.0
90-91	58891.0
92-93	60435.0
94-95	74586.0
96-97	256994.0
98-99	587124.0
100-101	5730887.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.8836589021469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02724427457217	77.31869139177616
2	7.023606138401488	12.064259877069972
3	1.7131539472959212	4.413957877692884
4	0.5974744569177252	2.0525316984266953
5	0.2527744177811042	1.0854595937950566
6	0.13723490142773226	0.7071741278213466
7	0.07921214855188978	0.47621204029956843
8	0.046946986924061254	0.32255832091757025
9	0.028540938817149617	0.22060802287072098
>10	0.0922432982769475	1.2159926352034498
>50	0.001262335582294816	0.06807419476225879
>100	2.843548817195979E-4	0.039651502733923656
>500	2.1800569861570792E-5	0.014828716630439475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2077724991409718E-5
2	0.0	2.4155449982819437E-5	0.0	0.0	1.2077724991409718E-5
3	0.0	2.4155449982819437E-5	0.0	0.0	1.2077724991409718E-5
4	0.0	3.6233174974229154E-5	0.0	0.0	1.2077724991409718E-5
5	0.0	3.6233174974229154E-5	0.0	0.0	1.2077724991409718E-5
6	0.0	3.6233174974229154E-5	0.0	0.0	1.2077724991409718E-5
7	0.0	4.8310899965638874E-5	0.0	0.0	1.2077724991409718E-5
8	0.0	4.8310899965638874E-5	0.0	1.2077724991409718E-5	2.4155449982819437E-5
9	0.0	1.0869952492268747E-4	0.0	1.2077724991409718E-5	2.4155449982819437E-5
10-11	0.0	1.0869952492268747E-4	0.0	1.2077724991409718E-5	2.4155449982819437E-5
12-13	0.0	1.0869952492268747E-4	0.0	1.2077724991409718E-5	3.0194312478524294E-5
14-15	0.0	1.4493269989691661E-4	0.0	2.4155449982819437E-5	6.642748745275345E-5
16-17	0.0	1.4493269989691661E-4	0.0	2.4155449982819437E-5	8.454407493986803E-5
18-19	0.0	1.5701042488832633E-4	0.0	2.4155449982819437E-5	9.058293743557289E-5
20-21	0.0	1.6908814987973605E-4	0.0	4.8310899965638874E-5	1.2077724991409719E-4
22-23	0.0	1.9928246235826036E-4	0.0	6.642748745275345E-5	1.2077724991409719E-4
24-25	0.0	2.234379123410798E-4	0.0	7.246634994845831E-5	1.2077724991409719E-4
26-27	0.0	2.2947677483678466E-4	0.0	8.454407493986803E-5	1.2077724991409719E-4
28-29	0.0	2.2947677483678466E-4	0.0	1.7512701237544091E-4	1.2681611240980203E-4
30-31	0.0	2.8986539979383323E-4	0.0	5.736919370919616E-4	1.328549749055069E-4
32-33	0.0	2.8986539979383323E-4	0.0	0.001968669173599784	1.328549749055069E-4
34-35	0.0	3.381762997594721E-4	0.0	0.003786366784806946	1.328549749055069E-4
36-37	0.0	3.985649247165207E-4	0.0	0.006600476707805411	1.328549749055069E-4
38-39	0.0	4.2272037469934015E-4	0.0	0.010882030217260155	1.328549749055069E-4
40-41	0.0	4.8310899965638875E-4	0.0	0.015652731588866993	1.328549749055069E-4
42-43	0.0	5.133033121349131E-4	0.0	0.020363044335516785	1.3889383740121175E-4
44-45	0.0	5.676530745962568E-4	0.0	0.025610815844284308	1.4493269989691661E-4
46-47	0.0	5.918085245790762E-4	0.0	0.031021636640435862	1.4493269989691661E-4
48-49	0.0	5.918085245790762E-4	0.0	0.03687329439877387	1.5701042488832633E-4
50-51	0.0	6.280416995533054E-4	0.0	0.04259813604470207	1.630492873840312E-4
52-53	0.0	6.401194245447151E-4	0.0	0.04808142319080209	1.6908814987973605E-4
54-55	0.0	6.401194245447151E-4	0.0	0.05334127242456102	1.6908814987973605E-4
56-57	0.0	6.582360120318296E-4	0.0	0.05784022498386114	1.8116587487114577E-4
58-59	6.038862495704859E-6	6.642748745275344E-4	0.0	0.06227878891820421	1.932435998625555E-4
60-61	1.2077724991409718E-5	6.703137370232394E-4	0.0	0.06666300309008594	1.932435998625555E-4
62-63	1.2077724991409718E-5	6.763525995189442E-4	0.0	0.07104721726196767	1.932435998625555E-4
64-65	1.2077724991409718E-5	7.488189494674025E-4	0.0	0.0754374702963451	1.932435998625555E-4
66-67	1.2077724991409718E-5	7.488189494674025E-4	0.0	0.07990018968067099	1.932435998625555E-4
68-69	1.2077724991409718E-5	7.608966744588122E-4	0.0	0.08419382091511715	2.0532132485396522E-4
70-71	1.2077724991409718E-5	7.72974399450222E-4	0.0	0.08825193651223082	2.1739904984537494E-4
72-73	1.2077724991409718E-5	7.72974399450222E-4	0.0	0.09131967866004888	2.4155449982819438E-4
74-75	1.2077724991409718E-5	7.910909869373365E-4	0.0	0.09352386347098116	2.4155449982819438E-4
76-77	1.2077724991409718E-5	8.092075744244511E-4	0.0	0.09601187481921156	2.4155449982819438E-4
78-79	1.2077724991409718E-5	8.092075744244511E-4	0.0	0.09734646343076234	2.4155449982819438E-4
80-81	1.2077724991409718E-5	8.15246436920156E-4	0.0	0.09778730039294878	2.4759336232389924E-4
82-83	1.2077724991409718E-5	8.212852994158609E-4	0.0	0.0981617098676825	2.5363222481960407E-4
84-85	1.2077724991409718E-5	8.212852994158609E-4	0.0	0.09832475915506651	2.5363222481960407E-4
86-87	1.2077724991409718E-5	8.333630244072705E-4	0.0	0.09846365299246773	2.5363222481960407E-4
88	1.2077724991409718E-5	9.058293743557288E-4	0.0	0.09848176957995484	2.777876748024235E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9750	0.0	51.03177	1
CTTATAC	9690	0.0	50.047245	1
TATACAC	14075	0.0	37.172165	3
GTATCAA	18340	0.0	36.654793	1
TTATACA	13820	0.0	36.317314	2
TCAACGC	21635	0.0	30.831259	4
ATCAACG	21600	0.0	30.800743	3
CAACGCA	22100	0.0	30.143219	5
TATCAAC	22990	0.0	29.27873	2
AACGCAG	23045	0.0	28.943289	6
ACATCTC	17660	0.0	27.926811	8
ACACATC	17605	0.0	27.816599	6
TACACAT	18775	0.0	27.589006	5
ACGCAGA	25680	0.0	26.041128	7
CACATCT	19120	0.0	25.612513	7
CGCAGAG	26735	0.0	25.094772	8
CATCTCC	20050	0.0	24.359486	9
ATACACA	23165	0.0	23.486149	4
GCAGAGT	31135	0.0	21.534422	9
GTACATG	25125	0.0	21.100538	1
>>END_MODULE