FastQCFastQC Report
Fri 27 May 2016
SRR2138968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10790987
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106150.047.212641
GTATCAA192450.036.6295781
TCAACGC237100.029.2724934
ATCAACG238450.029.0337243
CAACGCA243400.028.4790465
AACGCAG252900.027.5630556
TATCAAC256050.027.1230722
ACGCAGA295150.023.5437157
CGCAGAG304100.022.8651128
GCAGAGT368000.018.9539419
AGAGTAC328950.018.7023910-11
GTACATG291800.017.9657021
TACATGG296350.017.601712
ACATGGG293650.017.4225143
GAGTACT200400.017.14210312-13
CAGAGTA362350.016.46179410-11
AGTACTT226700.015.70077512-13
CATGGGG204850.015.4100834
GTACTTT227400.015.10704214-15
GTGGTAT61000.014.7755431
Produced by FastQC (version 0.11.5)