FastQCFastQC Report
Fri 27 May 2016
SRR2138967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9916648
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124300.048.468261
GTATCAA221000.036.6473961
ATCAACG269700.029.1325683
TCAACGC270900.029.0194724
CAACGCA273700.028.7231755
TATCAAC287200.027.6433432
AACGCAG284250.027.6268396
ACGCAGA317950.024.6579767
CGCAGAG320350.024.5003498
GCAGAGT382550.020.4515519
AGAGTAC352750.019.32415810-11
GTACATG250650.019.2252921
GAGTACT246350.018.87929312-13
TACATGG251100.018.4851682
CAGAGTA378950.018.02233510-11
ACATGGG252250.017.8526363
AGTACTT271050.017.47781812-13
GTACTTT280900.016.61163114-15
CATGGGG181450.016.2916454
GTGGTAT62300.014.9147591
Produced by FastQC (version 0.11.5)