##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9916648
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58553999294923	33.0	33.0	33.0	33.0	33.0
2	32.60003238997694	33.0	33.0	33.0	33.0	33.0
3	32.593581117329165	33.0	33.0	33.0	33.0	33.0
4	36.502275466468106	37.0	37.0	37.0	37.0	37.0
5	36.531172125903836	37.0	37.0	37.0	37.0	37.0
6	36.52193493204559	37.0	37.0	37.0	37.0	37.0
7	36.50189751617684	37.0	37.0	37.0	37.0	37.0
8	36.48684515170852	37.0	37.0	37.0	37.0	37.0
9	36.52255348783178	37.0	37.0	37.0	37.0	37.0
10-11	36.47746516766553	37.0	37.0	37.0	37.0	37.0
12-13	36.41430965382658	37.0	37.0	37.0	37.0	37.0
14-15	38.475127734694226	40.0	37.0	40.0	37.0	40.0
16-17	38.439362020311705	40.0	37.0	40.0	37.0	40.0
18-19	38.45217920410203	40.0	37.0	40.0	37.0	40.0
20-21	38.45053474475009	40.0	37.0	40.0	37.0	40.0
22-23	38.44481986743626	40.0	37.0	40.0	37.0	40.0
24-25	38.446941062678555	40.0	37.0	40.0	37.0	40.0
26-27	38.308060614306385	40.0	37.0	40.0	37.0	40.0
28-29	38.23491480930106	40.0	37.0	40.0	37.0	40.0
30-31	38.199622674915815	40.0	37.0	40.0	37.0	40.0
32-33	38.12494552847157	40.0	37.0	40.0	35.0	40.0
34-35	38.09251296690678	40.0	37.0	40.0	33.0	40.0
36-37	38.06176307567722	40.0	37.0	40.0	33.0	40.0
38-39	38.021553578285875	40.0	37.0	40.0	33.0	40.0
40-41	37.99349466278718	40.0	37.0	40.0	33.0	40.0
42-43	37.907064100164305	40.0	37.0	40.0	33.0	40.0
44-45	37.87014363302111	40.0	37.0	40.0	33.0	40.0
46-47	37.84518817658882	40.0	37.0	40.0	33.0	40.0
48-49	37.82760708187273	40.0	37.0	40.0	33.0	40.0
50-51	37.7782289744852	40.0	37.0	40.0	33.0	40.0
52-53	37.690345239427465	40.0	37.0	40.0	33.0	40.0
54-55	37.502398485174254	40.0	37.0	40.0	33.0	40.0
56-57	37.334468897635105	40.0	37.0	40.0	33.0	40.0
58-59	37.28786955465171	40.0	37.0	40.0	33.0	40.0
60-61	37.169471682669005	37.0	37.0	40.0	33.0	40.0
62-63	37.00264838004585	37.0	37.0	40.0	33.0	40.0
64-65	36.869371743005246	37.0	37.0	40.0	33.0	40.0
66-67	36.66483059103619	37.0	37.0	40.0	33.0	40.0
68-69	36.454268683640564	37.0	37.0	40.0	33.0	40.0
70-71	36.20277518310773	37.0	37.0	40.0	33.0	40.0
72-73	35.992187452110926	37.0	37.0	37.0	33.0	40.0
74-75	35.80793884099586	37.0	35.0	37.0	33.0	40.0
76-77	34.03101980090531	35.0	33.0	37.0	27.0	37.0
78-79	35.22986466677645	37.0	33.0	37.0	33.0	37.0
80-81	35.2719958246242	37.0	33.0	37.0	33.0	37.0
82-83	35.13866357225881	37.0	33.0	37.0	33.0	37.0
84-85	34.97429896486537	37.0	33.0	37.0	33.0	37.0
86-87	34.83993118561564	37.0	33.0	37.0	33.0	37.0
88-89	34.75483252240383	37.0	33.0	37.0	33.0	37.0
90-91	34.66736204266998	37.0	33.0	37.0	33.0	37.0
92-93	34.590485779611285	37.0	33.0	37.0	33.0	37.0
94-95	34.57329142472099	37.0	33.0	37.0	33.0	37.0
96-97	34.57036198049846	37.0	33.0	37.0	33.0	37.0
98-99	34.57067351215784	37.0	33.0	37.0	33.0	37.0
100	34.573071032402325	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	14.0
20	70.0
21	346.0
22	1415.0
23	3950.0
24	9195.0
25	17970.0
26	31567.0
27	50722.0
28	75004.0
29	105206.0
30	144031.0
31	191553.0
32	255536.0
33	343866.0
34	474087.0
35	681449.0
36	1157739.0
37	2719676.0
38	3560550.0
39	92702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.715113413322726	21.52080017360705	15.15768231362049	23.606404099449733
2	13.953646433754633	21.89832693466583	42.09819689072356	22.049829740855984
3	16.67499945546116	27.570364502198725	31.5421299616564	24.212506080683717
4	11.814586945104837	19.33226832292525	40.279931283231996	28.57321344873792
5	12.735976914780075	38.33686544082234	36.335554110622866	12.59160353377472
6	28.170729936458343	39.32118567531516	19.76742333394241	12.740661054284091
7	24.798954243409668	33.49310170130068	24.89885695247023	16.80908710281942
8	23.397270932678058	37.24320960066345	22.28559489053156	17.073924576126934
9	25.021821889815993	16.91432427570284	22.441998546282978	35.62185528819819
10-11	23.135766075047098	27.490043215437492	30.00011748120893	19.374073228306475
12-13	24.094894617083046	25.18181217262351	29.984597072702634	20.73869613759081
14-15	21.364742394174357	26.572436391463157	27.710962994128813	24.351858220233673
16-17	20.785687865496485	29.392335999019025	28.512179720405523	21.309796415078967
18-19	20.749849142573176	28.70604058952178	30.132631510163517	20.411478757741527
20-21	21.657713134142444	27.912866222203576	29.872113665626504	20.557306978027476
22-23	21.386353976409563	27.796213122887508	29.657394377507444	21.16003852319548
24-25	21.131967996436426	27.942726621467017	29.771660194443093	21.153645187653463
26-27	21.102901938300178	28.129713709197507	29.66207077103108	21.105313581471236
28-29	21.037314886885277	28.2174019704344	29.698084787838592	21.04719835484174
30-31	21.30380740745232	28.03813470685782	29.62997601991166	21.0280818657782
32-33	21.21748459416252	28.10840339852233	29.498008977293743	21.1761030300214
34-35	21.274526334868465	28.21548868019607	29.453786243550123	21.05619874138534
36-37	21.295945383710567	28.179811580563413	29.391287425209157	21.132955610516866
38-39	21.175791293607684	28.30822389181397	29.431911536135306	21.084073278443032
40-41	21.430218082424975	28.180441761783428	29.293189682821406	21.09615047297019
42-43	21.376222052239342	28.280240176191413	29.043440786847068	21.300096984722177
44-45	21.4592587665264	28.425458239677674	28.84678824230666	21.268494751489268
46-47	21.485263727358145	28.40777502914893	28.582487409149376	21.524473834343546
48-49	21.479483857432076	28.507530488500354	28.558959712292737	21.454025941774834
50-51	21.503415662117103	28.610509013104323	28.40106451898583	21.485010805792744
52-53	21.580311684811754	28.52239175002226	28.330706185741345	21.566590379424646
54-55	21.61549786506559	28.467059186483073	28.351230219762858	21.566212728688477
56-57	21.652942518650434	28.47934486557877	28.27323711100783	21.594475504762958
58-59	21.534699058222735	28.583023100221865	28.35976013980483	21.522517701750576
60-61	21.677409134998683	28.570660892125854	28.29031258258986	21.461617390285603
62-63	21.589897650848126	28.619634977743893	28.235613177159152	21.554854194248833
64-65	21.569536189392235	28.553553264754246	28.215497000466357	21.661413545387163
66-67	21.64604514526642	28.601457486476455	28.092431988804485	21.66006537945264
68-69	21.5828767737124	28.565964502851564	28.170639626568928	21.68051909686711
70-71	21.6244602151247	28.35494108784062	28.222549018992403	21.79804967804228
72-73	21.65470327184028	28.423584702284828	28.161992088721536	21.759719937153353
74-75	21.579950841969957	28.51648638635378	28.12203026756802	21.78153250410824
76-77	21.660499281473374	28.473793809479197	28.02190721611828	21.843799692929146
78-79	21.622318596111427	28.428438379982058	28.08259202493757	21.866650998968947
80-81	21.604640017493345	28.456539728725893	28.100162727839635	21.838657525941123
82-83	21.64745165470835	28.461122851652	28.16481967621064	21.72660581742901
84-85	21.778866237332085	28.469956657945712	28.02071285424446	21.73046425047774
86-87	21.643365334627756	28.461433832991517	28.07568539547739	21.819515436903338
88-89	21.689285846767707	28.40305343963765	28.09989320232127	21.80776751127338
90-91	21.682456574062854	28.447553243437955	28.12484985787898	21.745140324620213
92-93	21.75435688544501	28.386558381463338	28.125058780444483	21.73402595264717
94-95	21.553826615317558	28.510715637802885	28.174266129138463	21.76119161774109
96-97	21.619595728107193	28.64397247229415	28.240269963674724	21.49616183592393
98-99	22.690535966149504	30.49086741889986	28.504005641748943	18.314590973201692
100	21.59531645986485	31.62652243777892	22.338095822480796	24.44006527987543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1862.0
1	1833.5
2	3031.5
3	4513.0
4	6296.0
5	10434.0
6	13649.0
7	15140.5
8	15335.0
9	14252.0
10	12795.0
11	11447.0
12	12493.5
13	16692.5
14	26675.0
15	40797.5
16	52124.0
17	58005.5
18	59413.0
19	57301.0
20	52983.0
21	49068.0
22	48114.5
23	52372.0
24	62448.5
25	78471.0
26	98952.5
27	121619.0
28	145542.0
29	171689.0
30	196886.5
31	221673.0
32	247713.0
33	271889.5
34	296505.5
35	319591.5
36	341056.0
37	361088.0
38	373397.5
39	381340.5
40	391936.5
41	399381.0
42	401714.5
43	402935.0
44	404220.5
45	404696.0
46	401911.0
47	396962.0
48	389172.5
49	377108.0
50	362019.5
51	344176.0
52	322980.5
53	297841.5
54	271608.5
55	245033.0
56	219350.5
57	193595.5
58	167344.0
59	142626.5
60	116897.0
61	92980.5
62	72882.5
63	55679.0
64	41098.0
65	30527.0
66	23012.5
67	17049.5
68	12676.0
69	9047.5
70	6261.0
71	4414.5
72	3136.0
73	2235.5
74	1625.0
75	1117.5
76	723.5
77	484.0
78	318.5
79	207.0
80	141.0
81	101.5
82	83.5
83	64.0
84	43.0
85	30.0
86	22.5
87	17.5
88	14.0
89	12.0
90	8.5
91	5.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.033621038076576E-5
7	0.0
8	0.0
9	0.0
10-11	0.0016991628622897575
12-13	2.672273937725731E-4
14-15	0.007684048077535878
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	144.0
20-21	628.0
22-23	1425.0
24-25	3178.0
26-27	4301.0
28-29	7349.0
30-31	17040.0
32-33	21827.0
34-35	31035.0
36-37	43605.0
38-39	49856.0
40-41	54149.0
42-43	64554.0
44-45	77778.0
46-47	75451.0
48-49	66309.0
50-51	56113.0
52-53	54414.0
54-55	57526.0
56-57	61546.0
58-59	62195.0
60-61	61012.0
62-63	60713.0
64-65	65493.0
66-67	69732.0
68-69	70293.0
70-71	69282.0
72-73	67470.0
74-75	69967.0
76-77	69312.0
78-79	73976.0
80-81	76086.0
82-83	74170.0
84-85	76078.0
86-87	80579.0
88-89	86386.0
90-91	90685.0
92-93	96831.0
94-95	118494.0
96-97	336238.0
98-99	621969.0
100-101	6771459.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.18089633313127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51309453655568	69.36697464493957
2	8.769977701314586	14.063693458259555
3	2.4870943269979224	5.982523572112179
4	1.0151201482763497	3.255729734984753
5	0.4652542327590921	1.8652250702703648
6	0.25067865421075236	1.2059783511720716
7	0.1463211010794363	0.8212509925899933
8	0.09411277129853689	0.6036837087289342
9	0.06668636136718459	0.48122750048543605
>10	0.18932151574032852	2.1860580708216593
>50	0.002016849604817932	0.10521156804938701
>100	2.754718003441078E-4	0.04301545783331671
>500	4.632899487712445E-5	0.019427869752856104
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.016810519038288E-5	0.0	0.0	0.0
3	0.0	2.016810519038288E-5	0.0	0.0	0.0
4	0.0	8.067242076153152E-5	0.0	1.008405259519144E-5	0.0
5	0.0	8.067242076153152E-5	0.0	1.008405259519144E-5	0.0
6	0.0	8.067242076153152E-5	0.0	1.008405259519144E-5	2.016810519038288E-5
7	0.0	8.067242076153152E-5	0.0	1.008405259519144E-5	2.016810519038288E-5
8	0.0	1.2100863114229727E-4	0.0	1.008405259519144E-5	2.016810519038288E-5
9	0.0	1.3109268373748872E-4	0.0	5.04202629759572E-5	2.016810519038288E-5
10-11	0.0	1.4117673633268015E-4	0.0	5.04202629759572E-5	2.016810519038288E-5
12-13	0.0	1.4117673633268015E-4	0.0	5.04202629759572E-5	2.016810519038288E-5
14-15	0.0	1.6638686782065876E-4	0.0	5.04202629759572E-5	2.016810519038288E-5
16-17	0.0	1.7142889411825448E-4	0.0	5.546228927355292E-5	2.016810519038288E-5
18-19	0.0	1.9159699930863734E-4	0.0	6.554634186874436E-5	2.016810519038288E-5
20-21	0.0	2.1176510449902024E-4	0.0	8.067242076153152E-5	2.016810519038288E-5
22-23	0.0	2.319332096894031E-4	0.0	9.579849965431867E-5	2.016810519038288E-5
24-25	0.0	2.4705928858219026E-4	0.0	1.1092457854710584E-4	2.016810519038288E-5
26-27	0.0	2.52101314879786E-4	0.0	1.563028152254673E-4	2.016810519038288E-5
28-29	0.0	2.52101314879786E-4	0.0	3.630258934268918E-4	2.016810519038288E-5
30-31	0.0	2.9243752526055173E-4	0.0	0.0012504225218037384	2.016810519038288E-5
32-33	0.0	3.025215778557432E-4	0.0	0.003831939986172747	2.016810519038288E-5
34-35	0.0	3.630258934268918E-4	0.0	0.007426904736358495	2.016810519038288E-5
36-37	0.0	4.1344615640284904E-4	0.0	0.013356327662331063	2.016810519038288E-5
38-39	0.0	4.235302089980405E-4	0.0	0.02812946471428652	2.016810519038288E-5
40-41	0.0	4.840345245691891E-4	0.0	0.044334537234759164	2.016810519038288E-5
42-43	0.0	5.092446560571677E-4	0.0	0.05434800146178427	2.016810519038288E-5
44-45	0.0	5.798330242235078E-4	0.0	0.06650432686528754	2.016810519038288E-5
46-47	0.0	6.252112609018693E-4	0.0	0.07875140874214755	2.016810519038288E-5
48-49	0.0	6.352953134970607E-4	0.0	0.09008084183284513	2.016810519038288E-5
50-51	0.0	7.361358394489751E-4	0.0	0.1009514505304615	2.016810519038288E-5
52-53	0.0	7.361358394489751E-4	0.0	0.1265044398066766	2.5210131487978597E-5
54-55	0.0	7.411778657465708E-4	0.0	0.15729105237979607	3.0252157785574317E-5
56-57	0.0	7.56303944639358E-4	0.0	0.1888238848449597	3.0252157785574317E-5
58-59	0.0	7.56303944639358E-4	0.0	0.21857688202707204	3.0252157785574317E-5
60-61	0.0	7.56303944639358E-4	0.0	0.2305315263786715	3.0252157785574317E-5
62-63	0.0	7.613459709369537E-4	0.0	0.24508785629983035	3.0252157785574317E-5
64-65	0.0	8.067242076153152E-4	0.0	0.2506492113060784	3.0252157785574317E-5
66-67	0.0	8.168082602105066E-4	0.0	0.25520720307910494	3.0252157785574317E-5
68-69	0.0	8.420183916984852E-4	0.0	0.2598156151151074	3.0252157785574317E-5
70-71	0.0	8.621864968888681E-4	0.0	0.2641164635469566	3.0252157785574317E-5
72-73	0.0	8.672285231864638E-4	0.0	0.2674895791400481	4.033621038076576E-5
74-75	0.0	8.773125757816552E-4	0.0	0.2700257183677388	4.033621038076576E-5
76-77	0.0	8.974806809720382E-4	0.0	0.27265261406878616	4.033621038076576E-5
78-79	0.0	8.974806809720382E-4	0.0	0.2742963146418024	4.033621038076576E-5
80-81	0.0	8.974806809720382E-4	0.0	0.27476522308747875	4.033621038076576E-5
82-83	0.0	8.974806809720382E-4	0.0	0.27514337505979847	4.033621038076576E-5
84-85	0.0	8.974806809720382E-4	0.0	0.2753248880065119	4.033621038076576E-5
86-87	0.0	8.974806809720382E-4	0.0	0.2754307705587614	4.033621038076576E-5
88	0.0	9.88237154328761E-4	0.0	0.275435812585059	4.033621038076576E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12430	0.0	48.46826	1
GTATCAA	22100	0.0	36.647396	1
ATCAACG	26970	0.0	29.132568	3
TCAACGC	27090	0.0	29.019472	4
CAACGCA	27370	0.0	28.723175	5
TATCAAC	28720	0.0	27.643343	2
AACGCAG	28425	0.0	27.626839	6
ACGCAGA	31795	0.0	24.657976	7
CGCAGAG	32035	0.0	24.500349	8
GCAGAGT	38255	0.0	20.451551	9
AGAGTAC	35275	0.0	19.324158	10-11
GTACATG	25065	0.0	19.225292	1
GAGTACT	24635	0.0	18.879293	12-13
TACATGG	25110	0.0	18.485168	2
CAGAGTA	37895	0.0	18.022335	10-11
ACATGGG	25225	0.0	17.852636	3
AGTACTT	27105	0.0	17.477818	12-13
GTACTTT	28090	0.0	16.611631	14-15
CATGGGG	18145	0.0	16.291645	4
GTGGTAT	6230	0.0	14.914759	1
>>END_MODULE