FastQCFastQC Report
Fri 27 May 2016
SRR2138966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10068075
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122750.051.372861
GTATCAA223150.037.0499571
ATCAACG270450.029.992453
TCAACGC272150.029.7094214
CAACGCA275500.029.3017755
AACGCAG283850.028.4092256
TATCAAC287650.028.2744982
ACGCAGA321750.025.0764227
CGCAGAG326550.024.6549028
GTACATG282500.021.0470771
TACATGG280300.020.7477662
GCAGAGT386700.020.6770829
ACATGGG280400.020.1212523
GAGTACT239550.019.5880112-13
AGAGTAC354650.019.27766210-11
CAGAGTA385050.018.40953610-11
CATGGGG206250.017.21274
AGTACTT264850.016.96294412-13
GTACTTT277500.016.87843914-15
GTGGTAT60200.015.572091
Produced by FastQC (version 0.11.5)