##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10068075
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59100672174174	33.0	33.0	33.0	33.0	33.0
2	32.607558843175084	33.0	33.0	33.0	33.0	33.0
3	32.59935687805265	33.0	33.0	33.0	33.0	33.0
4	36.5108186023644	37.0	37.0	37.0	37.0	37.0
5	36.53877995545325	37.0	37.0	37.0	37.0	37.0
6	36.528616940179724	37.0	37.0	37.0	37.0	37.0
7	36.50975633375794	37.0	37.0	37.0	37.0	37.0
8	36.494193378575346	37.0	37.0	37.0	37.0	37.0
9	36.52803281660099	37.0	37.0	37.0	37.0	37.0
10-11	36.485165237644736	37.0	37.0	37.0	37.0	37.0
12-13	36.42097794265537	37.0	37.0	37.0	37.0	37.0
14-15	38.48965671193351	40.0	37.0	40.0	37.0	40.0
16-17	38.45260131653767	40.0	37.0	40.0	37.0	40.0
18-19	38.466669497396474	40.0	37.0	40.0	37.0	40.0
20-21	38.465750941579444	40.0	37.0	40.0	37.0	40.0
22-23	38.46048692433403	40.0	37.0	40.0	37.0	40.0
24-25	38.45984052489308	40.0	37.0	40.0	37.0	40.0
26-27	38.32276843952935	40.0	37.0	40.0	37.0	40.0
28-29	38.25127447428011	40.0	37.0	40.0	37.0	40.0
30-31	38.21483175488386	40.0	37.0	40.0	37.0	40.0
32-33	38.141387723598044	40.0	37.0	40.0	35.0	40.0
34-35	38.11003477425342	40.0	37.0	40.0	33.0	40.0
36-37	38.07963996917062	40.0	37.0	40.0	33.0	40.0
38-39	38.03920679715625	40.0	37.0	40.0	33.0	40.0
40-41	38.01234444515481	40.0	37.0	40.0	33.0	40.0
42-43	37.92488851270447	40.0	37.0	40.0	33.0	40.0
44-45	37.8865818870837	40.0	37.0	40.0	33.0	40.0
46-47	37.858599796945974	40.0	37.0	40.0	33.0	40.0
48-49	37.84126689028794	40.0	37.0	40.0	33.0	40.0
50-51	37.78987241901747	40.0	37.0	40.0	33.0	40.0
52-53	37.70419787600184	40.0	37.0	40.0	33.0	40.0
54-55	37.51546388405802	40.0	37.0	40.0	33.0	40.0
56-57	37.35301032053128	40.0	37.0	40.0	33.0	40.0
58-59	37.301761611658435	40.0	37.0	40.0	33.0	40.0
60-61	37.181862124469845	37.0	37.0	40.0	33.0	40.0
62-63	37.01549548651025	37.0	37.0	40.0	33.0	40.0
64-65	36.881328739424944	37.0	37.0	40.0	33.0	40.0
66-67	36.67759720352534	37.0	37.0	40.0	33.0	40.0
68-69	36.471984740517115	37.0	37.0	40.0	33.0	40.0
70-71	36.2218649833907	37.0	37.0	40.0	33.0	40.0
72-73	36.013819365410654	37.0	37.0	37.0	33.0	40.0
74-75	35.832603207136074	37.0	35.0	37.0	33.0	40.0
76-77	34.05115964127155	35.0	33.0	37.0	27.0	37.0
78-79	35.25290137854792	37.0	33.0	37.0	33.0	37.0
80-81	35.296620054351784	37.0	33.0	37.0	33.0	37.0
82-83	35.16158963399869	37.0	33.0	37.0	33.0	37.0
84-85	34.99750767817645	37.0	33.0	37.0	33.0	37.0
86-87	34.86425249487061	37.0	33.0	37.0	33.0	37.0
88-89	34.77814816526325	37.0	33.0	37.0	33.0	37.0
90-91	34.686039166167205	37.0	33.0	37.0	33.0	37.0
92-93	34.60795190860482	37.0	33.0	37.0	33.0	37.0
94-95	34.59041404693133	37.0	33.0	37.0	33.0	37.0
96-97	34.58872923654597	37.0	33.0	37.0	33.0	37.0
98-99	34.58651769577267	37.0	33.0	37.0	33.0	37.0
100	34.59102671681732	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	10.0
20	72.0
21	356.0
22	1341.0
23	3991.0
24	8888.0
25	17754.0
26	31355.0
27	50127.0
28	74969.0
29	105654.0
30	144658.0
31	193786.0
32	259481.0
33	347691.0
34	478926.0
35	690350.0
36	1176554.0
37	2755114.0
38	3633501.0
39	93493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.77736558378836	21.560169148521442	15.156700759579165	23.505764508111035
2	13.957653275328202	21.89543681388945	42.23962376124532	21.90728614953703
3	16.701921668243433	27.76307288136014	31.43008966460818	24.104915785788247
4	11.803686404799327	19.30414701916702	40.34402802919128	28.548138546842374
5	12.807850557330969	38.384845166528855	36.17207857510001	12.63522570104017
6	27.982442322136293	39.41156968055658	19.869122932548965	12.736865064758165
7	24.864236708606164	33.43975884168523	24.93780588642814	16.75819856328047
8	23.451275442425686	37.30101335160892	22.268457475733943	16.97925373023145
9	25.190058675566085	17.008037782793632	22.458662653983012	35.343240887657274
10-11	23.20380110800592	27.517915467331765	29.904404654532694	19.37387877012962
12-13	24.138439985121245	25.20382556891631	29.98982920715988	20.667905238802568
14-15	21.476727428568505	26.51302022661264	27.6791357968833	24.33111654793555
16-17	20.82905619991905	29.430635945798972	28.444325255820996	21.295982598460977
18-19	20.838387675896335	28.6733710267355	30.072888809429806	20.41535248793836
20-21	21.752419396291316	27.822929467828416	29.84449265620449	20.58015847967578
22-23	21.530439370905547	27.77643099489277	29.619187578513014	21.07394205568867
24-25	21.244238447848566	27.93254926201912	29.705077378622118	21.1181349115102
26-27	21.179896238457758	28.111402788274674	29.656034611694217	21.052666361573348
28-29	21.149382570045876	28.17455316754762	29.66698148741644	21.00908277499006
30-31	21.419481708474464	27.962544908679572	29.580185287282458	21.03778809556351
32-33	21.343829237565185	28.064445556317473	29.423758511808888	21.16796669430845
34-35	21.361197654887835	28.13953808929453	29.453724427745648	21.045539828071988
36-37	21.357584742909903	28.160170952570258	29.36376292824304	21.118481376276794
38-39	21.214248036027055	28.22812379677323	29.43414499378686	21.12348317341285
40-41	21.413164141482813	28.122697924066458	29.285074728411274	21.179063206039455
42-43	21.400029665111983	28.216033764365005	29.035640462298097	21.348296108224915
44-45	21.484507287335063	28.31644496857091	28.87643633876135	21.322611405332673
46-47	21.54720953070993	28.327110092525725	28.593676091874524	21.532004284889815
48-49	21.53240828741757	28.40784899799788	28.555709166245286	21.50403354833926
50-51	21.522152385066065	28.555709631244802	28.430936017911634	21.491201965777503
52-53	21.635970856543924	28.468989903268966	28.328078637841646	21.566960602345468
54-55	21.689234688517843	28.436638023577693	28.277582201354782	21.596545086549682
56-57	21.688847685724568	28.494664419008515	28.238219414618175	21.578268480648745
58-59	21.62102955289067	28.542897269400292	28.318293607772503	21.517779569936533
60-61	21.714601187775095	28.560257285129865	28.26506049301984	21.460081034075206
62-63	21.665285290797488	28.586095085516767	28.214363772612305	21.534255851073443
64-65	21.658272133669115	28.51690172888915	28.185059785641087	21.63976635180065
66-67	21.75745488106697	28.479871834877883	28.08176085053034	21.6809124335248
68-69	21.678576876793166	28.513475018891327	28.156409934074023	21.651538170241487
70-71	21.682968455914875	28.362610023667063	28.204155380540353	21.75026613987771
72-73	21.70950037820282	28.39411976249899	28.160217252128533	21.73616260716966
74-75	21.663607970189577	28.461077607590973	28.133452902020167	21.74186152019928
76-77	21.70771609431711	28.49066751355458	28.040169558209282	21.761446833919027
78-79	21.722463216291242	28.406341486095886	28.06405302198573	21.80714227562714
80-81	21.674658241490945	28.464388071291847	28.11124332587577	21.74971036134144
82-83	21.708544769483748	28.43914548436624	28.20431932887185	21.64799041727816
84-85	21.83266198742669	28.397719000586996	28.065707862362423	21.703911149623895
86-87	21.68509304105036	28.490037158194543	28.077422868080955	21.747446932674144
88-89	21.75558110078158	28.412186699258214	28.099547830223	21.732684369737207
90-91	21.805364435284876	28.426077012819846	28.103406136079258	21.66515241581602
92-93	21.80491019338961	28.452535320465728	28.061459441674565	21.6810950444701
94-95	21.628664186555977	28.535482047989607	28.098078668293486	21.737775097160934
96-97	21.700673733778157	28.605759342050668	28.18072073161008	21.512846192561096
98-99	22.77908308072672	30.519174474283833	28.450121884851203	18.251620560138246
100	21.727682441951217	31.619985396280033	22.286222276895558	24.366109884873193
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1612.0
1	1608.5
2	2814.5
3	4305.5
4	6123.5
5	10217.0
6	13337.5
7	14777.5
8	14980.0
9	13987.0
10	12644.5
11	11383.5
12	12484.0
13	16745.5
14	26692.0
15	40603.0
16	51589.0
17	57168.0
18	57978.0
19	55180.0
20	50661.0
21	46552.0
22	45723.0
23	50132.0
24	60082.5
25	75435.0
26	95718.5
27	119168.0
28	144486.5
29	173123.0
30	200710.0
31	225604.5
32	252316.0
33	278160.0
34	303065.0
35	324862.0
36	345395.5
37	365387.5
38	378504.5
39	386413.0
40	393522.0
41	399748.0
42	402194.0
43	403682.0
44	404499.0
45	404688.5
46	401869.5
47	396455.0
48	388600.5
49	376612.5
50	364094.5
51	347679.0
52	327731.5
53	305624.0
54	279646.0
55	251270.5
56	223950.0
57	198749.0
58	174525.0
59	148797.0
60	120790.0
61	95650.5
62	74932.0
63	57314.5
64	42573.5
65	31630.5
66	23848.0
67	17748.0
68	13212.5
69	9466.0
70	6599.0
71	4716.5
72	3310.5
73	2286.5
74	1487.5
75	951.5
76	625.0
77	402.5
78	271.5
79	179.0
80	127.5
81	104.5
82	85.5
83	66.5
84	48.0
85	32.0
86	23.0
87	17.5
88	12.5
89	11.0
90	9.0
91	6.0
92	5.5
93	4.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	9.932385287157673E-6
7	0.0
8	0.0
9	0.0
10-11	0.0016736069208860681
12-13	2.979715586147302E-4
14-15	0.007146351214109947
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	515.0
22-23	1188.0
24-25	2825.0
26-27	3919.0
28-29	6572.0
30-31	15302.0
32-33	19817.0
34-35	27575.0
36-37	39017.0
38-39	47409.0
40-41	49781.0
42-43	60593.0
44-45	74402.0
46-47	72446.0
48-49	63800.0
50-51	56334.0
52-53	54679.0
54-55	58841.0
56-57	62370.0
58-59	62033.0
60-61	61210.0
62-63	60403.0
64-65	64268.0
66-67	68794.0
68-69	68250.0
70-71	67875.0
72-73	65402.0
74-75	68479.0
76-77	67803.0
78-79	72641.0
80-81	74664.0
82-83	73479.0
84-85	74740.0
86-87	79141.0
88-89	85610.0
90-91	89638.0
92-93	95901.0
94-95	118920.0
96-97	339193.0
98-99	633622.0
100-101	6958501.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.4253878043184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.84153841829242	70.71105943233076
2	8.812536069625871	14.35128334017661
3	2.369129893722092	5.78721961065375
4	0.9467172985487727	3.0834729270156203
5	0.4245689142908766	1.7285344247896524
6	0.21287043848395057	1.0399834803378587
7	0.1306809722945089	0.7448524193408043
8	0.07650136110021338	0.4983322396114458
9	0.05175949434078334	0.37930832093283373
>10	0.13211685847435997	1.5459090460801612
>50	0.0012642426175353187	0.06569209369698309
>100	2.9512444087181113E-4	0.04865181961278784
>500	2.0913767766620473E-5	0.015700845420804972
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.932385287157675E-6	9.932385287157675E-6
2	0.0	2.9797155861473023E-5	0.0	2.9797155861473023E-5	9.932385287157675E-6
3	0.0	2.9797155861473023E-5	0.0	2.9797155861473023E-5	9.932385287157675E-6
4	0.0	4.966192643578837E-5	0.0	3.97295411486307E-5	1.986477057431535E-5
5	0.0	4.966192643578837E-5	0.0	7.94590822972614E-5	1.986477057431535E-5
6	0.0	4.966192643578837E-5	0.0	7.94590822972614E-5	2.9797155861473023E-5
7	0.0	4.966192643578837E-5	0.0	7.94590822972614E-5	2.9797155861473023E-5
8	0.0	6.952669701010373E-5	0.0	7.94590822972614E-5	2.9797155861473023E-5
9	0.0	1.887153204559958E-4	0.0	7.94590822972614E-5	2.9797155861473023E-5
10-11	0.0	1.887153204559958E-4	0.0	8.939146758441907E-5	2.9797155861473023E-5
12-13	0.0	1.986477057431535E-4	0.0	9.932385287157674E-5	2.9797155861473023E-5
14-15	0.0	2.4830963217894184E-4	0.0	9.932385287157674E-5	4.4695733792209536E-5
16-17	0.0	2.5824201746609953E-4	0.0	1.0429004551515557E-4	4.966192643578837E-5
18-19	0.0	2.7314059539683606E-4	0.0	1.0925623815873442E-4	6.952669701010373E-5
20-21	0.0	2.8803917332757253E-4	0.0	1.2912100873304976E-4	6.952669701010373E-5
22-23	0.0	3.476334850505186E-4	0.0	1.3905339402020745E-4	6.952669701010373E-5
24-25	0.0	4.3205875999135883E-4	0.0	1.3905339402020745E-4	6.952669701010373E-5
26-27	0.0	4.56889723209253E-4	0.0	1.5395197195094395E-4	6.952669701010373E-5
28-29	0.0	4.56889723209253E-4	0.0	2.2347866896104768E-4	6.952669701010373E-5
30-31	0.0	5.164840349321991E-4	0.0	5.164840349321991E-4	6.952669701010373E-5
32-33	0.0	5.164840349321991E-4	0.0	0.001618978801806701	6.952669701010373E-5
34-35	0.0	5.860107319423028E-4	0.0	0.0029151550817807775	7.94590822972614E-5
36-37	0.0	6.555374289524065E-4	0.0	0.004991023606796732	7.94590822972614E-5
38-39	0.0	6.903007774574584E-4	0.0	0.008209116439835818	7.94590822972614E-5
40-41	0.0	7.548612818239832E-4	0.0	0.011601026015400164	7.94590822972614E-5
42-43	0.0	7.747260523982986E-4	0.0	0.014843949811657143	7.94590822972614E-5
44-45	0.0	8.442527494084023E-4	0.0	0.01879207296330232	8.442527494084023E-5
46-47	0.0	8.939146758441907E-4	0.0	0.02276502707816539	8.939146758441907E-5
48-49	0.0	8.939146758441907E-4	0.0	0.026534367294641724	9.932385287157674E-5
50-51	0.0	9.634413728542944E-4	0.0	0.030472558060999744	9.932385287157674E-5
52-53	0.0	9.634413728542944E-4	0.0	0.03438591786413987	1.0925623815873442E-4
54-55	0.0	9.634413728542944E-4	0.0	0.03870650546405345	1.2415481608947092E-4
56-57	0.0	9.634413728542944E-4	0.0	0.04319594361384872	1.2912100873304976E-4
58-59	0.0	9.833061434286097E-4	0.0	0.04762082125927747	1.2912100873304976E-4
60-61	0.0	0.0010180694919336617	0.0	0.05203080032677548	1.2912100873304976E-4
62-63	0.0	0.00106773141836945	0.0	0.056480508935422113	1.2912100873304976E-4
64-65	0.0	0.0011918862344589209	0.0	0.06102954139694033	1.2912100873304976E-4
66-67	0.0	0.0012117510050332363	0.0	0.06563816817018149	1.2912100873304976E-4
68-69	0.0	0.001251480546181867	0.0	0.07027659209928414	1.2912100873304976E-4
70-71	0.0	0.0012862438946869188	0.0	0.07423961382886003	1.2912100873304976E-4
72-73	0.0	0.0013011424726176552	0.0	0.07745770666189912	1.2912100873304976E-4
74-75	0.0	0.0013508043990534437	0.0	0.08003019445127296	1.2912100873304976E-4
76-77	0.0	0.001460060637212178	0.0	0.08247356123191374	1.2912100873304976E-4
78-79	9.932385287157675E-6	0.0014997901783608087	0.0	0.08415213434544339	1.2912100873304976E-4
80-81	9.932385287157675E-6	0.0014997901783608087	0.0	0.08467855076566275	1.340872013766286E-4
82-83	9.932385287157675E-6	0.0015047563710043877	0.0	0.08515033906680274	1.3905339402020745E-4
84-85	9.932385287157675E-6	0.001524621141578703	0.0	0.085324155809328	1.3905339402020745E-4
86-87	9.932385287157675E-6	0.001589181645945228	0.0	0.08540361489162526	1.3905339402020745E-4
88	9.932385287157675E-6	0.0017183026546782776	0.0	0.08540858108426884	1.3905339402020745E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12275	0.0	51.37286	1
GTATCAA	22315	0.0	37.049957	1
ATCAACG	27045	0.0	29.99245	3
TCAACGC	27215	0.0	29.709421	4
CAACGCA	27550	0.0	29.301775	5
AACGCAG	28385	0.0	28.409225	6
TATCAAC	28765	0.0	28.274498	2
ACGCAGA	32175	0.0	25.076422	7
CGCAGAG	32655	0.0	24.654902	8
GTACATG	28250	0.0	21.047077	1
TACATGG	28030	0.0	20.747766	2
GCAGAGT	38670	0.0	20.677082	9
ACATGGG	28040	0.0	20.121252	3
GAGTACT	23955	0.0	19.58801	12-13
AGAGTAC	35465	0.0	19.277662	10-11
CAGAGTA	38505	0.0	18.409536	10-11
CATGGGG	20625	0.0	17.2127	4
AGTACTT	26485	0.0	16.962944	12-13
GTACTTT	27750	0.0	16.878439	14-15
GTGGTAT	6020	0.0	15.57209	1
>>END_MODULE