FastQCFastQC Report
Fri 27 May 2016
SRR2138965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22005426
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA284100.053.1015781
GTATCAA509500.038.983841
ATCAACG644100.030.0916183
TCAACGC647050.029.9748574
CAACGCA660200.029.365335
TATCAAC679700.028.9498522
AACGCAG699000.027.729026
ACGCAGA761150.025.4070387
CGCAGAG811950.023.8285068
TACATGG670300.021.8689772
GTACATG689200.021.787091
ACATGGG676950.021.1790183
GCAGAGT955150.020.2223039
GAGTACT548100.020.06793612-13
AGAGTAC841400.019.42869410-11
CATGGGG519650.019.2129424
CAGAGTA902800.018.86638610-11
AGTACTT585450.018.07634712-13
GTACTTT630500.017.4281814-15
GTGGTAT140400.017.1030791
Produced by FastQC (version 0.11.5)