FastQCFastQC Report
Fri 27 May 2016
SRR2138964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4862565
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57650.053.216451
GTATCAA101800.035.000281
TCAACGC124750.028.0516724
ATCAACG127000.027.5546953
CAACGCA130300.026.8243645
TATCAAC132850.026.5651152
AACGCAG133150.026.2792826
ACGCAGA154800.022.6011287
GTGGTAT28700.022.1165081
CGCAGAG158800.022.0051888
TGGTATC27250.021.2745932
GAGTACT105950.019.80547312-13
GTACATG136850.019.7589231
GCAGAGT183900.018.8637339
TACATGG141550.018.5049532
ACATGGG140000.018.1652033
CAGAGTA181750.017.88822410-11
AGAGTAC166700.017.75210610-11
GTACTTT118750.017.74299814-15
TATGCCG23050.017.09468742-43
Produced by FastQC (version 0.11.5)