FastQCFastQC Report
Fri 27 May 2016
SRR2138963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499837
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77400.049.4897541
GTATCAA138400.034.9091151
ATCAACG165150.028.5122743
TCAACGC165800.028.4004974
CAACGCA170850.027.5857495
TATCAAC174350.027.4203782
AACGCAG176650.026.7498866
ACGCAGA202500.023.2909327
CGCAGAG206200.022.832058
GTGGTAT37500.019.4833581
GCAGAGT244000.019.312269
TGGTATC34250.019.235922
GAGTACT155700.018.46902312-13
AGAGTAC219250.018.13208610-11
GTACATG160750.018.0490651
CAGAGTA237000.017.74508510-11
TACATGG160950.017.6068022
ACATGGG161400.016.77223
GTACTTT176700.016.35883714-15
AGTACTT170150.016.26770212-13
Produced by FastQC (version 0.11.5)