##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6499837
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45881612108119	34.0	31.0	34.0	31.0	34.0
2	32.5891966213922	34.0	31.0	34.0	31.0	34.0
3	32.63963419390363	34.0	31.0	34.0	31.0	34.0
4	36.16742989093419	37.0	35.0	37.0	35.0	37.0
5	36.09212815644454	37.0	35.0	37.0	35.0	37.0
6	36.107846089063464	37.0	35.0	37.0	35.0	37.0
7	36.062498028796725	37.0	35.0	37.0	35.0	37.0
8	36.05559139406111	37.0	35.0	37.0	35.0	37.0
9	37.7810806640228	39.0	38.0	39.0	35.0	39.0
10-11	37.7145557650138	39.0	38.0	39.0	35.0	39.0
12-13	37.704113426229	39.0	38.0	39.0	35.0	39.0
14-15	39.135637093668656	41.0	39.0	41.0	36.0	41.0
16-17	39.11119563459822	41.0	39.0	41.0	36.0	41.0
18-19	39.11181734249644	41.0	38.5	41.0	36.0	41.0
20-21	39.09343413444974	41.0	39.0	41.0	35.0	41.0
22-23	39.08254652534917	41.0	39.0	41.0	35.0	41.0
24-25	39.04698749033311	41.0	39.0	41.0	35.0	41.0
26-27	38.99591738169299	41.0	39.0	41.0	35.0	41.0
28-29	38.93919575156089	40.5	38.5	41.0	35.0	41.0
30-31	38.85912532528189	40.0	38.0	41.0	35.0	41.0
32-33	38.768702830019116	40.0	38.0	41.0	35.0	41.0
34-35	38.69098233485688	40.0	38.0	41.0	34.0	41.0
36-37	38.67001006245225	40.0	38.0	41.0	34.0	41.0
38-39	38.61711007395297	40.0	38.0	41.0	34.0	41.0
40-41	38.608918936511685	40.0	38.0	41.0	34.0	41.0
42-43	38.559585251510555	40.0	38.0	41.0	34.0	41.0
44-45	38.47624446058725	40.0	38.0	41.0	34.0	41.0
46-47	38.429393324828	40.0	38.0	41.0	33.5	41.0
48-49	38.38345852009958	40.0	38.0	41.0	33.0	41.0
50-51	38.32792833986973	40.0	38.0	41.0	33.0	41.0
52-53	38.207032027678366	40.0	37.5	41.0	33.0	41.0
54-55	38.089769665428506	40.0	37.0	41.0	33.0	41.0
56-57	37.978942824716256	40.0	37.0	41.0	33.0	41.0
58-59	37.86806249130953	40.0	37.0	41.0	33.0	41.0
60-61	37.74274349637814	40.0	36.0	41.0	33.0	41.0
62-63	37.54371504359173	39.0	36.0	41.0	32.0	41.0
64-65	37.34831320092279	39.0	35.5	41.0	32.0	41.0
66-67	37.12666376311602	39.0	35.0	41.0	32.0	41.0
68-69	36.8006634134493	38.0	35.0	40.5	31.5	41.0
70-71	36.47443681975146	37.0	35.0	40.0	31.0	41.0
72-73	36.16756409751156	37.0	35.0	39.5	31.0	41.0
74-75	35.898332464572974	36.5	35.0	39.0	31.0	41.0
76-77	35.60140285404493	36.0	35.0	39.0	31.0	40.5
78-79	35.31405826047231	35.5	34.5	37.5	31.0	39.5
80-81	35.02385856331518	35.0	34.0	37.0	31.0	39.0
82-83	34.7141278354284	35.0	34.0	37.0	31.0	39.0
84-85	34.470276517368774	35.0	34.0	36.0	31.0	37.5
86-87	34.29843141372231	35.0	34.0	36.0	31.0	37.0
88-89	34.14772658587282	35.0	34.0	36.0	31.0	37.0
90-91	34.00696934893425	35.0	34.0	35.0	31.0	36.0
92-93	33.94582700711334	35.0	34.0	35.0	31.0	36.0
94-95	33.887171217559334	35.0	34.0	35.0	31.0	36.0
96-97	33.874847735465295	35.0	34.0	35.0	31.0	36.0
98-99	34.0118401215755	35.0	34.0	35.0	31.0	36.0
100	31.31062615399352	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	12.0
19	28.0
20	75.0
21	170.0
22	747.0
23	2369.0
24	6426.0
25	13579.0
26	25201.0
27	40558.0
28	59838.0
29	81895.0
30	106829.0
31	134525.0
32	170045.0
33	214808.0
34	284008.0
35	391867.0
36	578574.0
37	1070591.0
38	1811617.0
39	1370224.0
40	135851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94615557282437	21.38073616307609	14.862772712608024	23.81033555149152
2	14.765742641315166	21.74695980319708	41.1696935932119	22.31760396227585
3	17.70886411693609	26.576396530121432	31.257707901013937	24.457031451928547
4	12.549853173241113	18.808656278611295	39.1298581795205	29.51163236862709
5	12.814123508382142	38.184109360563205	36.17128785157022	12.830479279484432
6	29.17231988306343	38.82569527253145	18.8932415278686	13.108743316536522
7	25.57139509806169	33.192416979071936	23.747164736592623	17.489023186273748
8	23.88232197207407	37.20600993532607	21.58734134409832	17.32432674850154
9	25.15987708614847	16.86186899763794	21.70662125834848	36.27163265786511
10-11	23.670539329075275	27.052617489520888	29.59324352079707	19.683599660606767
12-13	24.499433524168918	24.669768591084452	29.566469061680937	21.26432882306569
14-15	21.66883736229365	26.202020620057137	27.11792827404197	25.011213743607243
16-17	21.280024480674157	29.222512435342452	27.888429542905875	21.609033541077515
18-19	21.307393048789617	28.34887579561414	29.542501820640087	20.801229334956158
20-21	22.051746387695953	27.49682955771513	29.453606919007076	20.997817135581844
22-23	21.76850723122533	27.512278756942628	29.291048163749178	21.42816584808287
24-25	21.629097655725115	27.689269948162043	29.33866781427732	21.34296458183552
26-27	21.585142999248173	27.850185801050248	29.28951356811544	21.27515763158614
28-29	21.531485618246485	27.85485001134454	29.319978751623193	21.293685618785783
30-31	21.896856690426354	27.62257142312736	29.248307084637016	21.232264801809272
32-33	21.64387773226151	27.763868239804097	29.18461290901523	21.407641118919166
34-35	21.75961017278873	27.68602688927851	29.20743397054174	21.34692896739102
36-37	21.650085824224945	27.778490144898587	29.19186719454888	21.379556836327588
38-39	21.506745454737803	27.77245810710366	29.286593371975066	21.434203066183468
40-41	21.827120409540044	27.63966211984124	29.13613366794384	21.39708380267487
42-43	21.794681260306778	27.687419212860714	28.951767481417516	21.566132045414992
44-45	21.76951677628113	27.841515792374967	28.79173038086703	21.59723705047687
46-47	21.918562538897383	27.801530078349142	28.45823523841819	21.821672144335285
48-49	21.851657697507743	27.866715751548238	28.45713986862303	21.824486682320984
50-51	21.734152638035514	28.007951687136917	28.427017733192145	21.83087794163542
52-53	21.853889271891376	27.989389765337545	28.39487686352441	21.761844099246673
54-55	21.892272660776868	27.88317751847132	28.37664983149767	21.847899989254145
56-57	21.92199921243024	27.960692035591133	28.228588917267043	21.88871983471158
58-59	21.76570798250796	28.087743443016183	28.449997868090364	21.69655070638549
60-61	21.894553708636277	28.23586876570266	28.226850418777676	21.642727106883385
62-63	21.751409319170424	28.34036927304922	28.18894955060003	21.719271857180324
64-65	21.687433164255385	28.307804734229215	28.169029742825508	21.835732358689892
66-67	21.76091923227308	28.37107326200592	28.004936214024355	21.86307129169664
68-69	21.7610381214992	28.274783055035396	28.0439722615413	21.9202065619241
70-71	21.774847993187866	27.948363753401136	28.200913461877875	22.075874791533128
72-73	21.8807539176617	27.936470207418022	28.133495784512665	22.049280090407613
74-75	21.790827251447315	28.098077652204168	28.05742645271642	22.053668643632097
76-77	21.921011178344134	28.036266951591287	27.936822476096406	22.10589939396817
78-79	21.897291110176695	28.090693622208978	27.948230929094986	22.06378433851934
80-81	21.88754361254128	28.06545647593927	27.950804814298458	22.096195097220996
82-83	21.916342589491187	28.077703787201436	28.026793047407107	21.979160575900273
84-85	22.046849945520243	28.103345300953265	27.9556724921753	21.89413226135119
86-87	21.871645709796194	28.18696185693208	28.039032173317736	21.902360259953987
88-89	21.917701948395376	28.168834336198916	28.01024226240607	21.903221452999638
90-91	21.95870258195485	28.174919899885047	28.061770844373424	21.80460667378668
92-93	21.99746105445162	28.11524822676696	28.053155461644657	21.834135257136765
94-95	21.87011865273001	28.148282622979583	28.110880544429534	21.870718179860873
96-97	21.872016547355493	28.25978055362539	28.238169879719255	21.63003301929986
98-99	22.799835622502236	30.17669876131828	28.51821533405215	18.50525028212733
100	21.882702553414322	31.208151656503947	22.01949850462004	24.889647285461695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1475.0
1	1229.5
2	1500.5
3	2041.5
4	2507.5
5	3999.5
6	5415.0
7	6810.0
8	7787.0
9	7570.0
10	6457.0
11	5134.0
12	5288.0
13	7266.0
14	12741.0
15	20609.5
16	27037.0
17	30695.5
18	31969.0
19	31497.5
20	29745.0
21	27588.5
22	27215.0
23	30346.0
24	36598.5
25	45589.5
26	57717.0
27	71292.5
28	84985.0
29	100444.0
30	116133.0
31	132347.0
32	149880.0
33	167030.5
34	183671.0
35	199451.0
36	217216.5
37	232838.5
38	242354.0
39	250225.5
40	259255.5
41	266818.5
42	270574.0
43	274973.5
44	281058.0
45	286765.5
46	286336.0
47	282310.5
48	277099.5
49	267665.0
50	257948.5
51	246483.5
52	232062.5
53	216177.0
54	199643.0
55	182051.5
56	164537.5
57	147402.5
58	129777.5
59	112090.5
60	92945.0
61	75187.5
62	60283.5
63	46837.0
64	35606.5
65	27199.0
66	21034.0
67	16511.5
68	12924.5
69	9851.0
70	7575.0
71	5873.0
72	4593.5
73	3525.0
74	2659.0
75	1972.5
76	1468.0
77	1123.0
78	842.5
79	631.0
80	479.5
81	355.0
82	269.0
83	196.0
84	139.5
85	100.0
86	73.5
87	59.0
88	41.5
89	30.5
90	21.5
91	12.0
92	7.5
93	3.5
94	1.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005230900405656326
3	2.0000501551038896E-4
4	0.0
5	0.00926177071825032
6	0.009030995700353718
7	0.0
8	0.0
9	0.0
10-11	3.077000238621368E-5
12-13	1.538500119310684E-5
14-15	7.69250059655342E-6
16-17	2.4616001908970946E-4
18-19	9.615625745691777E-4
20-21	0.0
22-23	1.231028693740065E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.102631962693953E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.3829544409294983E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.004467596080516962
52-53	4.9830439472086356E-5
54-55	1.6738085286905008E-5
56-57	0.0
58-59	8.510616568638549E-6
60-61	7.730005674683055E-5
62-63	8.756016293472538E-4
64-65	0.0012255258118495742
66-67	0.0012387276886341159
68-69	0.007077827921091614
70-71	8.145822532576049E-4
72-73	4.666012327055626E-4
74-75	4.345231007272622E-4
76-77	1.2150156115486673E-4
78-79	0.0
80-81	8.608910949807752E-5
82-83	0.0
84-85	2.9387615001084403E-5
86-87	1.0909256801921617E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	191.0
20-21	709.0
22-23	1451.0
24-25	2486.0
26-27	3778.0
28-29	6239.0
30-31	14541.0
32-33	18531.0
34-35	25267.0
36-37	36543.0
38-39	40395.0
40-41	43196.0
42-43	51313.0
44-45	61286.0
46-47	61301.0
48-49	54165.0
50-51	47039.0
52-53	44912.0
54-55	47773.0
56-57	50531.0
58-59	52783.0
60-61	54416.0
62-63	54572.0
64-65	59748.0
66-67	63016.0
68-69	63878.0
70-71	60680.0
72-73	56564.0
74-75	57938.0
76-77	60153.0
78-79	61496.0
80-81	62801.0
82-83	60795.0
84-85	61698.0
86-87	65034.0
88-89	68478.0
90-91	71798.0
92-93	76094.0
94-95	96673.0
96-97	232608.0
98-99	511178.0
100-101	3935789.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.3045462226454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.33443126612521	78.86606529969812
2	7.179357947352144	12.535811755270437
3	1.546485124549214	4.050455461165211
4	0.49005262456065546	1.7113528804993776
5	0.1986208411114981	0.8670251201799749
6	0.09036430192415013	0.47335286245284297
7	0.055548129510204915	0.33947229682836244
8	0.032852605691157714	0.22945454656784176
9	0.016549921968672755	0.13003950847456486
>10	0.05471295195195882	0.6851931504148665
>50	6.913401627862643E-4	0.03897443094712483
>100	3.08257379582978E-4	0.05594785394442465
>500	2.4687712759088427E-5	0.016854833556910524
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.538500119310684E-5	0.0	0.0	0.0
3	0.0	1.538500119310684E-5	0.0	0.0	0.0
4	0.0	3.077000238621368E-5	0.0	0.0	0.0
5	0.0	3.077000238621368E-5	0.0	0.0	0.0
6	0.0	3.077000238621368E-5	0.0	0.0	1.538500119310684E-5
7	0.0	3.077000238621368E-5	0.0	3.077000238621368E-5	3.077000238621368E-5
8	0.0	4.615500357932053E-5	0.0	3.077000238621368E-5	4.615500357932053E-5
9	0.0	1.2308000954485473E-4	0.0	3.077000238621368E-5	7.692500596553422E-5
10-11	0.0	1.2308000954485473E-4	0.0	3.077000238621368E-5	7.692500596553422E-5
12-13	0.0	1.2308000954485473E-4	0.0	3.077000238621368E-5	7.692500596553422E-5
14-15	0.0	1.6923501312417528E-4	0.0	3.077000238621368E-5	7.692500596553422E-5
16-17	0.0	1.8462001431728212E-4	0.0	3.077000238621368E-5	9.231000715864106E-5
18-19	0.0	1.9231251491383554E-4	0.0	5.3847504175873943E-5	1.076950083517479E-4
20-21	0.0	2.0000501551038896E-4	0.0	9.231000715864106E-5	1.2308000954485473E-4
22-23	0.0	2.3077501789660264E-4	0.0	1.3846501073796157E-4	1.46157511334515E-4
24-25	0.0	2.4616001908970946E-4	0.0	1.8462001431728212E-4	1.8462001431728212E-4
26-27	0.0	2.4616001908970946E-4	0.0	3.846250298276711E-4	1.9231251491383554E-4
28-29	0.0	2.4616001908970946E-4	0.0	0.0010615650823243721	2.0769751610694238E-4
30-31	0.0	2.615450202828163E-4	0.0	0.0034231627654662724	2.153900167034958E-4
32-33	0.0	2.769300214759232E-4	0.0	0.009584855743305563	2.153900167034958E-4
34-35	0.0	3.615475280380108E-4	0.0	0.018946628969311076	2.3077501789660264E-4
36-37	0.0	4.307800334069916E-4	0.0	0.03287005504907277	2.4616001908970946E-4
38-39	0.0	4.4616503460009844E-4	0.0	0.06687090768583889	2.6923752087936977E-4
40-41	0.0	4.769350369863121E-4	0.0	0.10200255791029836	2.769300214759232E-4
42-43	0.0	5.000125387759724E-4	0.0	0.12534929722083799	2.769300214759232E-4
44-45	0.0	5.461675423552929E-4	0.0	0.15721163469176228	2.9231502266903003E-4
46-47	0.0	5.692450441449532E-4	0.0	0.18855857462271747	3.077000238621369E-4
48-49	0.0	5.692450441449532E-4	0.0	0.21290533901080905	3.230850250552437E-4
50-51	0.0	6.154000477242737E-4	0.0	0.2365982408481936	3.230850250552437E-4
52-53	0.0	6.154000477242737E-4	0.0	0.3101923940554202	3.538550274414574E-4
54-55	0.0	6.154000477242737E-4	0.0	0.39494836562824576	3.923175304242245E-4
56-57	0.0	6.615550513035943E-4	0.0	0.5149513749344792	4.0770253161733134E-4
58-59	0.0	6.692475519001477E-4	0.0	0.5682219415656116	4.1539503221388476E-4
60-61	0.0	7.384800572691285E-4	0.0	0.5871224155313433	4.4616503460009844E-4
62-63	0.0	7.538650584622353E-4	0.0	0.5992765664738977	4.769350369863121E-4
64-65	0.0	8.7694506800709E-4	0.0	0.6056613419690371	4.769350369863121E-4
66-67	0.0	8.923300692001969E-4	0.0	0.6115076424224177	4.8462753758286555E-4
68-69	0.0	9.077150703933037E-4	0.0	0.6176385653978708	4.923200381794189E-4
70-71	0.0	9.231000715864106E-4	0.0	0.6224079157677338	5.000125387759724E-4
72-73	1.538500119310684E-5	9.384850727795174E-4	0.0	0.6267234086024003	6.000150465311669E-4
74-75	1.538500119310684E-5	9.384850727795174E-4	0.0	0.6301850338708495	6.154000477242737E-4
76-77	1.538500119310684E-5	9.538700739726242E-4	0.0	0.6333004966124536	6.230925483208271E-4
78-79	1.538500119310684E-5	9.538700739726242E-4	0.0	0.6359159468152817	6.307850489173806E-4
80-81	1.538500119310684E-5	9.538700739726242E-4	0.0	0.6367621218809025	6.538625507070408E-4
82-83	1.538500119310684E-5	9.538700739726242E-4	0.0	0.6373929069298199	6.92325053689808E-4
84-85	1.538500119310684E-5	9.615625745691776E-4	0.0	0.6376006044459268	7.077100548829148E-4
86-87	1.538500119310684E-5	9.846400763588378E-4	0.0	0.6376929144530855	7.230950560760216E-4
88	1.538500119310684E-5	0.0010615650823243721	0.0	0.6376929144530855	7.384800572691285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7740	0.0	49.489754	1
GTATCAA	13840	0.0	34.909115	1
ATCAACG	16515	0.0	28.512274	3
TCAACGC	16580	0.0	28.400497	4
CAACGCA	17085	0.0	27.585749	5
TATCAAC	17435	0.0	27.420378	2
AACGCAG	17665	0.0	26.749886	6
ACGCAGA	20250	0.0	23.290932	7
CGCAGAG	20620	0.0	22.83205	8
GTGGTAT	3750	0.0	19.483358	1
GCAGAGT	24400	0.0	19.31226	9
TGGTATC	3425	0.0	19.23592	2
GAGTACT	15570	0.0	18.469023	12-13
AGAGTAC	21925	0.0	18.132086	10-11
GTACATG	16075	0.0	18.049065	1
CAGAGTA	23700	0.0	17.745085	10-11
TACATGG	16095	0.0	17.606802	2
ACATGGG	16140	0.0	16.7722	3
GTACTTT	17670	0.0	16.358837	14-15
AGTACTT	17015	0.0	16.267702	12-13
>>END_MODULE