FastQCFastQC Report
Fri 27 May 2016
SRR2138962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4653784
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62700.051.925551
GTATCAA117450.038.7643281
ATCAACG142500.031.4636463
TCAACGC145400.030.984124
CAACGCA146550.030.7396625
TATCAAC148750.030.4631562
AACGCAG155250.029.2100546
ACGCAGA170050.026.5162897
CGCAGAG171750.026.203828
GTGGTAT27750.025.0744511
GCAGAGT198400.022.640719
TGGTATC29000.021.772022
CTTATAC60100.021.6544691
TATACAC72600.020.34913
TACATGG143900.020.23712
GAGTACT119400.020.10494412-13
ACATGGG143850.019.5847193
CAGAGTA197650.019.4891710-11
AGAGTAC186950.019.28381510-11
GTATAAT42850.018.8445781
Produced by FastQC (version 0.11.5)