FastQCFastQC Report
Fri 27 May 2016
SRR2138961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5686030
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73850.052.338011
GTATCAA131850.036.3375131
ATCAACG160900.029.0895213
TCAACGC162200.028.8825594
CAACGCA166650.028.0603455
TATCAAC170000.027.7831942
AACGCAG172600.027.1885876
GTGGTAT34300.024.2710321
ACGCAGA199050.023.5958277
CGCAGAG209100.022.421128
GTACATG187650.021.1635881
TACATGG185750.021.1514032
TGGTATC35200.021.1164882
ACATGGG190900.020.0905953
GCAGAGT233900.019.953079
GAGTACT134850.018.86398312-13
CATGGGG131600.018.2349074
ATCTCGT113450.018.23337694
CAGAGTA231500.018.22472810-11
TATCTCG126550.018.01956794
Produced by FastQC (version 0.11.5)