##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5686030
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47633814805761	34.0	31.0	34.0	31.0	34.0
2	32.60365140528629	34.0	31.0	34.0	31.0	34.0
3	32.65702520035948	34.0	31.0	34.0	31.0	34.0
4	36.17720114033869	37.0	35.0	37.0	35.0	37.0
5	36.101011953858844	37.0	35.0	37.0	35.0	37.0
6	36.11769951970004	37.0	36.0	37.0	35.0	37.0
7	36.068689753659406	37.0	35.0	37.0	35.0	37.0
8	36.06296273498381	37.0	35.0	37.0	35.0	37.0
9	37.79621475792425	39.0	38.0	39.0	35.0	39.0
10-11	37.726360307631154	39.0	38.0	39.0	35.0	39.0
12-13	37.712830568955845	39.0	38.0	39.0	35.0	39.0
14-15	39.14483295023065	41.0	39.0	41.0	36.0	41.0
16-17	39.120002268718245	41.0	39.0	41.0	36.0	41.0
18-19	39.118533493491945	41.0	38.5	41.0	36.0	41.0
20-21	39.106647186150894	41.0	39.0	41.0	35.5	41.0
22-23	39.09366717215004	41.0	39.0	41.0	35.0	41.0
24-25	39.05302128796268	41.0	39.0	41.0	35.0	41.0
26-27	38.99679924156546	41.0	39.0	41.0	35.0	41.0
28-29	38.93891362602365	41.0	38.5	41.0	35.0	41.0
30-31	38.849290553991864	40.0	38.0	41.0	35.0	41.0
32-33	38.7550068382392	40.0	38.0	41.0	35.0	41.0
34-35	38.672864706955785	40.0	38.0	41.0	34.0	41.0
36-37	38.64295916192425	40.0	38.0	41.0	34.0	41.0
38-39	38.59022253079365	40.0	38.0	41.0	34.0	41.0
40-41	38.59122623857824	40.0	38.0	41.0	34.0	41.0
42-43	38.54404980348774	40.0	38.0	41.0	34.0	41.0
44-45	38.45985994264373	40.0	38.0	41.0	33.5	41.0
46-47	38.4035224109648	40.0	38.0	41.0	33.0	41.0
48-49	38.347855430075654	40.0	38.0	41.0	33.0	41.0
50-51	38.26939139608329	40.0	38.0	41.0	33.0	41.0
52-53	38.14340463811883	40.0	37.0	41.0	33.0	41.0
54-55	38.03524990242319	40.0	37.0	41.0	33.0	41.0
56-57	37.91503579934255	40.0	37.0	41.0	33.0	41.0
58-59	37.8071298082818	40.0	36.0	41.0	33.0	41.0
60-61	37.67130506597917	39.5	36.0	41.0	32.5	41.0
62-63	37.473511200265676	39.0	35.5	41.0	32.0	41.0
64-65	37.28187447457648	39.0	35.0	41.0	32.0	41.0
66-67	37.05353760751365	39.0	35.0	41.0	32.0	41.0
68-69	36.72783509034407	38.0	35.0	40.5	31.5	41.0
70-71	36.40031356891631	37.0	35.0	40.0	31.0	41.0
72-73	36.099464957083015	37.0	35.0	39.0	31.0	41.0
74-75	35.82359991403392	36.0	35.0	39.0	31.0	41.0
76-77	35.51611576191709	36.0	35.0	39.0	31.0	40.5
78-79	35.223987091037124	35.5	34.0	37.5	31.0	39.5
80-81	34.92082064263532	35.0	34.0	37.0	31.0	39.0
82-83	34.60966716058165	35.0	34.0	36.5	31.0	39.0
84-85	34.38982219585006	35.0	34.0	36.0	31.0	37.0
86-87	34.221411337936075	35.0	34.0	36.0	31.0	37.0
88-89	34.074697236342416	35.0	34.0	35.5	31.0	37.0
90-91	33.94518355785982	35.0	34.0	35.0	31.0	36.0
92-93	33.884363294744425	35.0	34.0	35.0	31.0	36.0
94-95	33.810654418844265	35.0	34.0	35.0	31.0	36.0
96-97	33.8018023043693	35.0	34.0	35.0	31.0	36.0
98-99	33.95616715228361	35.0	34.0	35.0	31.0	36.0
100	31.108816964122735	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	10.0
19	29.0
20	57.0
21	159.0
22	676.0
23	2285.0
24	6008.0
25	12439.0
26	23185.0
27	37025.0
28	54203.0
29	73932.0
30	96401.0
31	120536.0
32	150888.0
33	190242.0
34	250859.0
35	348129.0
36	513638.0
37	960225.0
38	1606257.0
39	1131064.0
40	107781.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.59498454985289	21.056026788462248	14.451629695938994	23.89735896574587
2	14.843807023631781	21.52035698035648	41.257008163123885	22.378827832887858
3	17.849093038716006	26.618284845990694	31.010144178177356	24.522477937115944
4	12.401112903027244	18.842672303874583	38.41791197021472	30.33830282288345
5	12.764324322612996	38.4342801241125	35.9050332891448	12.896362264129705
6	29.318306936004433	38.45517144339598	18.804196603670707	13.422325016928882
7	25.985441511916047	33.04117283939761	23.15346559902076	17.819920049665583
8	24.30803214193383	36.96864068603226	21.356464879713965	17.366862292319947
9	25.401730205433314	16.525431628042764	21.102210153657296	36.97062801286663
10-11	23.870878899350892	26.955607751740164	29.275144954037764	19.89836839487118
12-13	24.832556707628758	24.475752368150747	29.238961915340045	21.45272900888045
14-15	21.893018669706162	25.974662987590904	26.657283434776414	25.475034907926524
16-17	21.494104394729877	29.2152412542	27.351783278798948	21.938871072271176
18-19	21.60461985769901	28.133419838564215	29.085024277274474	21.1769360264623
20-21	22.40427821519309	27.216962519624772	28.97756147819398	21.401197786988156
22-23	22.069002578495837	27.4377832446936	28.81399833787856	21.679215838932006
24-25	21.97672807441597	27.521847396589138	28.933083426769706	21.568341102225187
26-27	21.895425900811244	27.61891375540807	28.959079482198174	21.526580861582513
28-29	21.838669835001216	27.482140244692456	29.02262093279849	21.65656898750784
30-31	21.888288370936028	27.104849728884815	29.232215205561502	21.774646694617655
32-33	21.658776063842772	27.503176112099155	29.006288188966906	21.831759635091167
34-35	21.970190678045086	27.41946879843664	29.000224214331148	21.610116309187124
36-37	21.83018354767254	27.419588343484243	29.142047607022498	21.608180501820723
38-39	21.702676341946457	27.384858538671164	29.046895979263365	21.865569140119014
40-41	21.98678221815158	27.238419151919885	29.136153760602262	21.638644869326274
42-43	22.008674301280514	27.217753717132492	28.86985703479872	21.90371494678827
44-45	21.743746154464233	27.482202759576644	28.728715865309297	22.045335220649825
46-47	22.107565548126722	27.231015207710524	28.520061462001305	22.141357782161446
48-49	21.995329937770283	27.50878153975674	28.26938778798116	22.226500734491815
50-51	21.826237121754875	27.58618570615242	28.315613367224675	22.27196380486803
52-53	21.843344271650736	27.450809825057753	28.505589697744238	22.200256205547277
54-55	21.98134655084872	27.262047098419917	28.493863766961482	22.262742583769878
56-57	22.04701039042926	27.198175439518042	28.292258930840823	22.462555239211873
58-59	21.49185700286698	27.2759000794276	28.707121817773217	22.5251210999322
60-61	21.916194065979667	27.80011104195004	28.459811651335958	21.823883240734336
62-63	21.221722430094207	28.242072662444656	28.493333942828038	22.0428709646331
64-65	21.09368521939775	28.467750869988922	28.15127439896603	22.287289511647298
66-67	21.212605483816787	28.95427038641538	27.689945408304734	22.143178721463094
68-69	21.408499386760347	28.752308586946384	27.519766378755904	22.319425647537365
70-71	21.44091524790516	28.045513331543614	27.586399079689162	22.927172340862068
72-73	21.678636386688733	27.44594775630627	27.59718444833984	23.278231408665153
74-75	21.785033359635726	27.46955579577342	27.810710872508	22.93469997208285
76-77	22.149355596815244	27.369053592635247	27.134705966996446	23.34688484355306
78-79	22.282829081321985	27.43650825868011	26.74211040202712	23.538552257970785
80-81	22.12827236792992	27.83178470698502	26.91046989056028	23.129473034524786
82-83	22.119506189489023	27.561775025872095	27.00702744488812	23.311691339750766
84-85	22.218969866393913	28.012751253720392	26.94782014851424	22.820458731371463
86-87	22.674263232378127	27.945718579125234	26.989644494097938	22.3903736943987
88-89	22.40144938181373	27.981351056893967	27.337001965452334	22.28019759583996
90-91	22.36982142515192	27.604615477255876	27.23846101846795	22.787102079124246
92-93	22.352100815432028	27.165320342272242	27.512786479756755	22.969792362538975
94-95	21.600448518038334	27.698837395286795	28.114114659574668	22.586599427100207
96-97	21.683320097457408	27.583716050222485	28.301152223401232	22.431811628918876
98-99	22.778896545877274	29.36889674441166	28.745539012811903	19.10666769689916
100	21.992629043577665	30.535409241703874	21.928347107269058	25.543614607449406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1580.0
1	1229.0
2	1348.0
3	1821.5
4	2216.5
5	3593.0
6	4875.5
7	6094.5
8	6952.5
9	6691.5
10	5587.0
11	4292.5
12	4412.5
13	6202.0
14	11093.0
15	18429.5
16	24455.5
17	27496.0
18	28323.0
19	27597.0
20	25562.5
21	23076.0
22	21950.0
23	23790.5
24	28387.0
25	35393.0
26	44316.0
27	54867.0
28	66969.0
29	80289.5
30	92954.5
31	105899.5
32	120643.0
33	134992.5
34	148466.0
35	161656.5
36	175769.5
37	188116.0
38	196853.0
39	204311.5
40	211373.5
41	217632.0
42	222185.5
43	227294.0
44	233780.0
45	241768.0
46	252208.5
47	257421.5
48	255291.5
49	247420.0
50	237234.5
51	227451.0
52	214453.5
53	200460.0
54	186520.5
55	172017.0
56	157098.5
57	140779.5
58	123774.0
59	106728.5
60	88853.0
61	73252.5
62	59606.5
63	47232.0
64	36743.5
65	27357.0
66	20585.0
67	15672.5
68	11505.5
69	8365.0
70	6315.0
71	4873.0
72	3730.0
73	2872.0
74	2220.0
75	1729.5
76	1316.0
77	966.5
78	718.5
79	566.0
80	444.5
81	340.0
82	272.5
83	184.0
84	113.0
85	86.0
86	70.0
87	56.0
88	39.5
89	24.5
90	17.5
91	14.5
92	12.5
93	7.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005961980503092667
3	1.758696313596657E-4
4	0.0
5	0.009549720982829848
6	0.008441742305263953
7	0.0
8	0.0
9	0.0
10-11	6.155437097588299E-5
12-13	8.793481567983285E-6
14-15	8.793481567983285E-6
16-17	2.6380444703949854E-4
18-19	9.496960093421949E-4
20-21	0.0
22-23	1.6710897774512996E-4
24-25	0.0
26-27	2.6403904398151165E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.213378237713733E-5
36-37	0.0
38-39	8.967891629845251E-6
40-41	0.0
42-43	6.361719750976843E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.004197478903414309
52-53	2.8458842679422807E-5
54-55	4.750580734741919E-5
56-57	0.0
58-59	0.0
60-61	1.0566264357752194E-4
62-63	6.114320320506848E-4
64-65	9.518441440585693E-4
66-67	0.0011031515250175387
68-69	0.00681778517450962
70-71	7.757662069299196E-4
72-73	4.751300901022243E-4
74-75	3.6540637312563497E-4
76-77	8.44571322517401E-5
78-79	0.0
80-81	1.4056775749769494E-4
82-83	0.0
84-85	2.215836182344705E-5
86-87	1.907252980166925E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	169.0
20-21	675.0
22-23	1276.0
24-25	2190.0
26-27	3563.0
28-29	6429.0
30-31	14393.0
32-33	18565.0
34-35	25479.0
36-37	35555.0
38-39	24246.0
40-41	40881.0
42-43	47769.0
44-45	56852.0
46-47	57112.0
48-49	50350.0
50-51	27665.0
52-53	8237.0
54-55	8326.0
56-57	8020.0
58-59	29540.0
60-61	53339.0
62-63	54871.0
64-65	62781.0
66-67	66577.0
68-69	67023.0
70-71	60424.0
72-73	51627.0
74-75	52488.0
76-77	55026.0
78-79	56253.0
80-81	56840.0
82-83	54905.0
84-85	55581.0
86-87	58289.0
88-89	62187.0
90-91	65192.0
92-93	69706.0
94-95	87193.0
96-97	219141.0
98-99	485316.0
100-101	3423979.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.36604949647477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.10789473119681	77.82262896377682
2	7.14745988018642	12.34595747572496
3	1.6313885119368254	4.226897429097486
4	0.552367553378603	1.9082321382137237
5	0.2306262626222313	0.9959139606409309
6	0.11727090760069991	0.6076935006203141
7	0.06350863195277871	0.383949275548096
8	0.04402590591573601	0.30418748555564784
9	0.02647318608404403	0.20577460496975403
>10	0.07733215926667888	1.0039326553975056
>50	9.768480632747645E-4	0.05859257034826433
>100	6.754217959077386E-4	0.13623994010644414
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.758696313596657E-5	0.0	1.758696313596657E-5	0.0
5	0.0	1.758696313596657E-5	0.0	1.758696313596657E-5	0.0
6	0.0	1.758696313596657E-5	0.0	1.758696313596657E-5	0.0
7	0.0	1.758696313596657E-5	0.0	1.758696313596657E-5	0.0
8	0.0	3.517392627193314E-5	0.0	1.758696313596657E-5	0.0
9	0.0	1.0552177881579943E-4	0.0	3.517392627193314E-5	0.0
10-11	0.0	1.0552177881579943E-4	0.0	3.517392627193314E-5	0.0
12-13	0.0	1.0552177881579943E-4	0.0	3.517392627193314E-5	0.0
14-15	0.0	1.0552177881579943E-4	0.0	3.517392627193314E-5	0.0
16-17	0.0	1.0552177881579943E-4	0.0	3.517392627193314E-5	0.0
18-19	0.0	1.23108741951766E-4	0.0	3.517392627193314E-5	0.0
20-21	0.0	1.4069570508773256E-4	0.0	4.396740783991643E-5	0.0
22-23	0.0	2.0225007606361557E-4	0.0	7.914133411184956E-5	0.0
24-25	0.0	2.46217483903532E-4	0.0	2.1104355763159883E-4	0.0
26-27	0.0	2.46217483903532E-4	0.0	3.2535881801538154E-4	0.0
28-29	0.0	2.46217483903532E-4	0.0	0.0013981635693093423	0.0
30-31	0.0	2.8139141017546513E-4	0.0	0.005073838864726355	0.0
32-33	0.0	2.8139141017546513E-4	0.0	0.012099830637545	0.0
34-35	0.0	3.2535881801538154E-4	0.0	0.022555280221877128	0.0
36-37	0.0	3.6932622585529796E-4	0.0	0.04173386352164867	0.0
38-39	0.0	3.6932622585529796E-4	0.0	0.2102785247351843	0.0
40-41	0.0	4.0450015212723113E-4	0.0	0.3860426343160342	0.0
42-43	0.0	4.1329363369521443E-4	0.0	0.42780287828238683	0.0
44-45	0.0	4.6605452310311414E-4	0.0	0.48962105370530934	0.0
46-47	0.0	5.100219309430305E-4	0.0	0.5559326982094712	0.0
48-49	0.0	5.100219309430305E-4	0.0	0.6078054459790048	0.0
50-51	0.0	6.1554370975883E-4	0.0	0.6579107039533735	0.0
52-53	0.0	6.1554370975883E-4	0.0	1.1721886799753078	0.0
54-55	0.0	6.1554370975883E-4	0.0	1.8764674122366571	0.0
56-57	0.0	6.1554370975883E-4	0.0	2.6371387417934833	0.0
58-59	0.0	6.1554370975883E-4	0.0	3.4166193284242254	0.0
60-61	0.0	6.419241544627798E-4	0.0	3.624215841281175	0.0
62-63	0.0	6.507176360307631E-4	0.0	3.6310307894963625	0.0
64-65	0.0	7.034785254386629E-4	0.0	3.636825693849663	0.0
66-67	0.0	7.210654885746294E-4	0.0	3.641996261011637	0.0
68-69	0.0	7.210654885746294E-4	0.0	3.6474394261022187	0.0
70-71	0.0	7.210654885746294E-4	0.0	3.652442917114401	0.0
72-73	0.0	7.210654885746294E-4	0.0	3.6563296359674498	0.0
74-75	0.0	7.650328964145458E-4	0.0	3.659240278366452	0.0
76-77	0.0	7.738263779825291E-4	0.0	3.662660942696398	0.0
78-79	0.0	7.738263779825291E-4	0.0	3.6648065521989857	0.0
80-81	0.0	7.738263779825291E-4	0.0	3.665677106874216	0.0
82-83	0.0	7.826198595505124E-4	0.0	3.6664421397706306	0.0
84-85	0.0	7.914133411184957E-4	0.0	3.6666971507361024	0.0
86-87	0.0	7.914133411184957E-4	0.0	3.6667587051070782	0.0
88	0.0	8.090003042544623E-4	0.0	3.6667762920702143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7385	0.0	52.33801	1
GTATCAA	13185	0.0	36.337513	1
ATCAACG	16090	0.0	29.089521	3
TCAACGC	16220	0.0	28.882559	4
CAACGCA	16665	0.0	28.060345	5
TATCAAC	17000	0.0	27.783194	2
AACGCAG	17260	0.0	27.188587	6
GTGGTAT	3430	0.0	24.271032	1
ACGCAGA	19905	0.0	23.595827	7
CGCAGAG	20910	0.0	22.42112	8
GTACATG	18765	0.0	21.163588	1
TACATGG	18575	0.0	21.151403	2
TGGTATC	3520	0.0	21.116488	2
ACATGGG	19090	0.0	20.090595	3
GCAGAGT	23390	0.0	19.95307	9
GAGTACT	13485	0.0	18.863983	12-13
CATGGGG	13160	0.0	18.234907	4
ATCTCGT	11345	0.0	18.233376	94
CAGAGTA	23150	0.0	18.224728	10-11
TATCTCG	12655	0.0	18.019567	94
>>END_MODULE