FastQCFastQC Report
Fri 27 May 2016
SRR2138960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6993479
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107800.051.065591
GTATCAA191400.035.9676741
ATCAACG238600.028.1866323
TCAACGC244100.027.568614
CAACGCA245750.027.298735
TATCAAC250250.027.2928662
AACGCAG257550.026.1104776
ACGCAGA287650.023.3462757
TGGTATC45400.023.22252
GTGGTAT49150.023.1463991
CGCAGAG291500.023.052228
GCAGAGT334900.020.0026639
GAGTACT216450.018.93902412-13
TACATGG220550.018.4977912
ACATGGG217400.018.3043373
CAGAGTA332900.018.22693410-11
AGAGTAC317950.018.17988410-11
TGCGCGA7550.017.85649194
GTGATCG8050.017.5954348
GTACATG241550.017.424431
Produced by FastQC (version 0.11.5)