##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6993479
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47074610505015	34.0	31.0	34.0	31.0	34.0
2	32.604281645801755	34.0	31.0	34.0	31.0	34.0
3	32.65282215046331	34.0	31.0	34.0	31.0	34.0
4	36.175341628966066	37.0	35.0	37.0	35.0	37.0
5	36.093664397934134	37.0	35.0	37.0	35.0	37.0
6	36.109273939336916	37.0	36.0	37.0	35.0	37.0
7	36.06829776710561	37.0	35.0	37.0	35.0	37.0
8	36.06896653296592	37.0	35.0	37.0	35.0	37.0
9	37.80096286840927	39.0	38.0	39.0	35.0	39.0
10-11	37.73344432720825	39.0	38.0	39.0	35.0	39.0
12-13	37.70997853857858	39.0	38.0	39.0	35.0	39.0
14-15	39.13376225194928	41.0	39.0	41.0	36.0	41.0
16-17	39.113677041140754	41.0	39.0	41.0	36.0	41.0
18-19	39.12761960105979	41.0	38.5	41.0	35.5	41.0
20-21	39.11325445235508	41.0	39.0	41.0	35.0	41.0
22-23	39.09851438719135	41.0	39.0	41.0	35.0	41.0
24-25	39.053190718931724	41.0	39.0	41.0	35.0	41.0
26-27	38.99619393323805	41.0	39.0	41.0	35.0	41.0
28-29	38.943146593379794	41.0	38.5	41.0	35.0	41.0
30-31	38.8544952965311	40.0	38.0	41.0	35.0	41.0
32-33	38.748931490731636	40.0	38.0	41.0	35.0	41.0
34-35	38.66788341427377	40.0	38.0	41.0	34.0	41.0
36-37	38.649248508075914	40.0	38.0	41.0	34.0	41.0
38-39	38.60005931189485	40.0	38.0	41.0	34.0	41.0
40-41	38.59404465524016	40.0	38.0	41.0	34.0	41.0
42-43	38.55038681167664	40.0	38.0	41.0	34.0	41.0
44-45	38.46879155169037	40.0	38.0	41.0	34.0	41.0
46-47	38.42336227572143	40.0	38.0	41.0	33.5	41.0
48-49	38.37712245225861	40.0	38.0	41.0	33.0	41.0
50-51	38.327135404818605	40.0	38.0	41.0	33.0	41.0
52-53	38.208390327604526	40.0	37.5	41.0	33.0	41.0
54-55	38.10627751520576	40.0	37.0	41.0	33.0	41.0
56-57	37.99914027925756	40.0	37.0	41.0	33.0	41.0
58-59	37.896076811873876	40.0	37.0	41.0	33.0	41.0
60-61	37.77948636658881	40.0	36.0	41.0	33.0	41.0
62-63	37.58990292843393	39.0	36.0	41.0	32.5	41.0
64-65	37.40520618345815	39.0	35.5	41.0	32.0	41.0
66-67	37.19320833570595	39.0	35.0	41.0	32.0	41.0
68-69	36.879067361371725	38.5	35.0	40.5	31.5	41.0
70-71	36.57622339230585	37.5	35.0	40.0	31.0	41.0
72-73	36.276127773631345	37.0	35.0	39.5	31.0	41.0
74-75	36.01363018777224	36.5	35.0	39.0	31.0	41.0
76-77	35.719841974410016	36.0	35.0	39.0	31.0	41.0
78-79	35.43290859154307	36.0	35.0	38.5	31.0	39.5
80-81	35.14515322581517	35.0	34.5	37.0	31.0	39.0
82-83	34.83122109903104	35.0	34.0	37.0	31.0	39.0
84-85	34.57523915520851	35.0	34.0	36.5	31.0	38.0
86-87	34.39490981181797	35.0	34.0	36.0	31.0	37.0
88-89	34.23322317013134	35.0	34.0	36.0	31.0	37.0
90-91	34.08472918632886	35.0	34.0	35.5	31.0	36.5
92-93	34.016067795511916	35.0	34.0	35.0	31.0	36.0
94-95	33.946807839695914	35.0	34.0	35.0	31.0	36.0
96-97	33.92994122357946	35.0	34.0	35.0	31.0	36.0
98-99	34.05873103027855	35.0	34.0	35.0	31.0	36.0
100	31.32305274836617	33.0	29.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	13.0
19	40.0
20	93.0
21	227.0
22	772.0
23	2531.0
24	6814.0
25	14377.0
26	26520.0
27	43143.0
28	63522.0
29	86901.0
30	112580.0
31	142282.0
32	178273.0
33	227463.0
34	302654.0
35	423132.0
36	605377.0
37	1137122.0
38	1885991.0
39	1565989.0
40	167662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08751581294517	21.39510249476691	14.980970129459173	23.536411562828743
2	14.289516057531982	21.716035223895965	42.21875366438915	21.775695054182908
3	17.33089639125904	27.318479082754983	31.601378086739274	23.7492464392467
4	11.887445433095602	18.989876140330157	40.28502838143934	28.837650045134904
5	12.070005025242045	38.806766715794105	36.616396132079494	12.50683212688436
6	27.888118664483986	39.80682213241011	19.301255051216117	13.003804151889783
7	24.607108993964236	33.8581841741428	24.31146214923931	17.223244682653654
8	23.428811325521963	37.41005013384612	21.871474841062653	17.28966369956927
9	24.877689630583006	17.10403362904214	22.165934865894357	35.8523418744805
10-11	23.346590456558566	27.594637170973563	29.69724770511018	19.361524667357692
12-13	24.29041744322353	25.047086728520483	29.731815843275694	20.93067998498029
14-15	21.462734383573835	26.45718099471972	27.845331596476857	24.23475302522958
16-17	20.686608975118386	29.41720592671163	28.440807489115006	21.455377609054977
18-19	20.550964289746144	28.512550705760702	30.484592533987172	20.45189247050599
20-21	21.78409354969023	27.689464219960463	30.23912942446635	20.287312805882962
22-23	21.22341169535008	27.514695917293828	30.22170233226803	21.04019005508806
24-25	21.007892808979086	27.594124533084262	30.46864170240793	20.929340955528723
26-27	21.062429326920704	27.88608263553899	30.151957899687503	20.899530137852803
28-29	20.919677675641186	28.09793433920692	30.13319933590485	20.84918864924705
30-31	21.361717055197822	27.81582504046004	30.13922127337213	20.683236630970008
32-33	21.216494553133487	28.03490708358481	29.85024867185438	20.898349691427327
34-35	21.190373991104448	28.00298617485963	30.04789347287688	20.758746361159044
36-37	21.125239919741748	27.92831280860044	30.073335383048505	20.8731118886093
38-39	21.01098178344479	27.973736775408796	30.072417173100447	20.94286426804597
40-41	21.443399285692337	27.858094591570566	29.839863248346916	20.85864287439018
42-43	21.32310237961526	27.885472353380198	29.664742157627522	21.12668310937702
44-45	21.30936700724051	28.312034326719605	29.315252832149607	21.063345833890278
46-47	21.38231557944505	27.992528064972184	28.955336486333266	21.669819869249494
48-49	21.463935921618766	28.096288246620425	29.06078520388139	21.378990627879414
50-51	21.165437022762283	28.262510910011073	28.97824026746212	21.593811799764527
52-53	21.350632625708887	28.255828802352884	28.827618558379587	21.565920013558646
54-55	21.43445587645301	28.066841543472208	28.91114711913938	21.587555460935405
56-57	21.551051474823222	28.25031639386343	28.58551509942621	21.61311703188714
58-59	21.361279709606045	28.453096222988812	28.93027358910713	21.255350478298013
60-61	21.398996692273045	28.56487761459144	28.612754646626037	21.42337104650948
62-63	21.26875882535802	28.53516510005585	28.601659891777402	21.59441618280873
64-65	21.160239187453524	28.596335314359916	28.683442357184802	21.55998314100176
66-67	21.436495790601974	28.75107389473004	28.24973506108071	21.56269525358728
68-69	21.40406418110837	28.504979047359107	28.563032936190215	21.527923835342307
70-71	21.404386218801758	28.023835010889137	28.637554113636828	21.93422465667228
72-73	21.538292654109288	28.12460786912746	28.696545199393146	21.640554277370104
74-75	21.244840508609254	28.42062522509773	28.621049330834413	21.713484935458602
76-77	21.35510490979613	28.388594491730352	28.39879528857055	21.857505309902976
78-79	21.53268120172114	28.45046017757145	28.348441361128817	21.66841725957859
80-81	21.355128187888088	28.24271095160979	28.46973230105719	21.932428559444933
82-83	21.510935974322305	28.22386455176762	28.62129541072442	21.64390406318565
84-85	21.874368814380933	28.179531447197864	28.377861519434816	21.568238218986384
86-87	21.275610079590717	28.520434668763446	28.47845030872138	21.725504942924452
88-89	21.570245745399465	28.379243586984416	28.502797562045405	21.547713105570715
90-91	21.700384050355982	28.24973972417358	28.606382339448533	21.443493886021905
92-93	21.644264371490557	28.367681402632318	28.45034933451918	21.537704891357947
94-95	21.378234431578615	28.52341065042822	28.50478018727955	21.59357473071361
96-97	21.34771313439835	28.6426399110682	28.678530508919998	21.331116445613446
98-99	22.202192924985365	30.586416507280223	28.9672489684637	18.244141599270712
100	21.448258526989697	31.76324932347087	22.347412658946666	24.441079490592767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2582.0
1	1957.5
2	2133.5
3	2959.0
4	3776.5
5	6453.5
6	8864.5
7	10666.5
8	11566.0
9	10685.5
10	8709.0
11	6755.0
12	7168.0
13	10373.0
14	18911.0
15	31317.5
16	41430.5
17	46518.5
18	47644.0
19	46222.5
20	42809.5
21	39071.5
22	38056.0
23	41629.5
24	49522.0
25	63020.5
26	83507.5
27	105107.5
28	119574.0
29	132570.0
30	143222.5
31	152155.0
32	167194.5
33	186240.5
34	208208.5
35	227121.0
36	249050.0
37	268975.0
38	272870.5
39	275571.5
40	287521.5
41	293993.5
42	291282.5
43	294901.0
44	302142.0
45	305928.0
46	303510.0
47	299716.0
48	292081.0
49	278457.5
50	266665.5
51	255333.5
52	241669.0
53	223643.5
54	203618.0
55	183522.0
56	164338.0
57	145606.5
58	127735.0
59	113254.5
60	96003.0
61	74616.5
62	56148.0
63	41408.0
64	29885.0
65	21897.5
66	16475.0
67	12576.5
68	9663.0
69	7219.5
70	5737.5
71	4507.0
72	3434.5
73	2536.5
74	1932.0
75	1529.0
76	1159.0
77	876.0
78	601.5
79	377.5
80	278.5
81	209.5
82	169.0
83	129.0
84	86.5
85	57.0
86	36.0
87	24.5
88	16.0
89	10.5
90	7.0
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007135218394164049
3	2.1448552287066283E-4
4	0.0
5	0.01116754622413251
6	0.012239973838485822
7	0.0
8	0.0
9	0.0
10-11	8.579420914826513E-5
12-13	1.4299034858044187E-5
14-15	2.144855228706628E-5
16-17	3.217282843059942E-4
18-19	9.651848529179827E-4
20-21	0.0
22-23	2.00226198396703E-4
24-25	0.0
26-27	7.156314080319894E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.332811325853264E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.2294187860636356E-5
44-45	0.0
46-47	7.599298706318186E-6
48-49	0.0
50-51	0.0062616473632871065
52-53	1.1758755490946855E-4
54-55	3.163376952347285E-5
56-57	0.0
58-59	8.062198247036236E-6
60-61	1.140509926876206E-4
62-63	0.0011443197081079167
64-65	0.0015474364136746788
66-67	0.0012203200285066758
68-69	0.010212693176626271
70-71	9.917932130124305E-4
72-73	6.719081904648549E-4
74-75	5.029105728830796E-4
76-77	9.817030191294651E-5
78-79	0.0
80-81	1.6466041670611657E-4
82-83	0.0
84-85	9.381299542642886E-6
86-87	2.470221010673825E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	260.0
20-21	804.0
22-23	1568.0
24-25	3012.0
26-27	4801.0
28-29	8402.0
30-31	19419.0
32-33	24008.0
34-35	32021.0
36-37	47124.0
38-39	53031.0
40-41	55735.0
42-43	65813.0
44-45	77798.0
46-47	77991.0
48-49	69837.0
50-51	59616.0
52-53	55712.0
54-55	57966.0
56-57	61075.0
58-59	63162.0
60-61	64581.0
62-63	63551.0
64-65	67241.0
66-67	71244.0
68-69	72241.0
70-71	69640.0
72-73	63122.0
74-75	63891.0
76-77	66009.0
78-79	69055.0
80-81	71400.0
82-83	66249.0
84-85	66455.0
86-87	68803.0
88-89	74357.0
90-91	77328.0
92-93	81399.0
94-95	100485.0
96-97	232673.0
98-99	530956.0
100-101	4113644.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.27983938974359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55624474845604	70.2900126597513
2	8.00716160372382	12.856272950293398
3	2.308384850347022	5.5595029510672855
4	0.9379414317849074	3.01191150002714
5	0.4803129428431559	1.9279722954131822
6	0.23559448696536003	1.1348092544812887
7	0.14370846925905398	0.807582498174393
8	0.08927157098743112	0.5733365904752851
9	0.05869955740610241	0.42411519367298406
>10	0.17457618693346982	2.1271815493367714
>50	0.0040472571480060894	0.22747165152733703
>100	0.003383291182341784	0.5591344084519201
>500	5.052022360771533E-4	0.27162745471571476
>1k	1.6840072736334593E-4	0.22906904261200461
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	7.1495174290220935E-6	0.0	0.0	0.0
18-19	0.0	1.4299034858044187E-5	0.0	7.1495174290220935E-6	0.0
20-21	0.0	1.4299034858044187E-5	0.0	2.8598069716088374E-5	0.0
22-23	0.0	2.144855228706628E-5	0.0	7.149517429022093E-5	0.0
24-25	0.0	2.8598069716088374E-5	0.0	1.5728938343848606E-4	0.0
26-27	0.0	2.8598069716088374E-5	0.0	2.073360054416407E-4	0.0
28-29	0.0	2.8598069716088374E-5	0.0	9.508858180599384E-4	0.0
30-31	0.0	2.8598069716088374E-5	0.0	0.003832141341955842	0.0
32-33	0.0	2.8598069716088374E-5	0.0	0.010581285794952698	0.0
34-35	0.0	2.8598069716088374E-5	0.0	0.019503883546372273	0.0
36-37	0.0	2.8598069716088374E-5	0.0	0.03434628172902214	0.0
38-39	0.0	2.8598069716088374E-5	0.0	0.06847092841774459	0.0
40-41	0.0	2.8598069716088374E-5	0.0	0.10224524875244495	0.0
42-43	0.0	2.8598069716088374E-5	0.0	0.12556697460591504	0.0
44-45	0.0	2.8598069716088374E-5	0.0	0.15525892048864376	0.0
46-47	0.0	2.8598069716088374E-5	0.0	0.18460054001735046	0.0
48-49	0.0	5.004662200315466E-5	0.0	0.20799376104511075	0.0
50-51	0.0	7.149517429022093E-5	0.0	0.23109385185828113	0.0
52-53	0.0	7.149517429022093E-5	0.0	0.29411684799511084	0.0
54-55	0.0	7.149517429022093E-5	0.0	0.36217310440197215	0.0
56-57	0.0	7.149517429022093E-5	0.0	0.4626452728320196	0.0
58-59	1.4299034858044187E-5	7.149517429022093E-5	0.0	0.5194195907358841	0.0
60-61	1.4299034858044187E-5	7.149517429022093E-5	0.0	0.5429915496993699	0.0
62-63	1.4299034858044187E-5	7.149517429022093E-5	0.0	0.5593210475072564	0.0
64-65	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.565984397751105	0.0
66-67	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5717397592814677	0.0
68-69	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5774736722595435	0.0
70-71	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5830574453716098	0.0
72-73	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5875187442473195	0.0
74-75	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5910863534444015	0.0
76-77	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5945395703626193	0.0
78-79	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5967988178701902	0.0
80-81	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5975566667176666	0.0
82-83	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5982716184605688	0.0
84-85	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5985075525357264	0.0
86-87	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5985647486751586	0.0
88	1.4299034858044187E-5	8.579420914826513E-5	0.0	0.5985718981925877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10780	0.0	51.06559	1
GTATCAA	19140	0.0	35.967674	1
ATCAACG	23860	0.0	28.186632	3
TCAACGC	24410	0.0	27.56861	4
CAACGCA	24575	0.0	27.29873	5
TATCAAC	25025	0.0	27.292866	2
AACGCAG	25755	0.0	26.110477	6
ACGCAGA	28765	0.0	23.346275	7
TGGTATC	4540	0.0	23.2225	2
GTGGTAT	4915	0.0	23.146399	1
CGCAGAG	29150	0.0	23.05222	8
GCAGAGT	33490	0.0	20.002663	9
GAGTACT	21645	0.0	18.939024	12-13
TACATGG	22055	0.0	18.497791	2
ACATGGG	21740	0.0	18.304337	3
CAGAGTA	33290	0.0	18.226934	10-11
AGAGTAC	31795	0.0	18.179884	10-11
TGCGCGA	755	0.0	17.856491	94
GTGATCG	805	0.0	17.595434	8
GTACATG	24155	0.0	17.42443	1
>>END_MODULE